GONDO Takahiro

写真a

Title

Assistant Professor

Research Fields, Keywords

Genetic Transformation

Graduating School 【 display / non-display

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    -
    1997.03

    University of Miyazaki   Faculty of Agriculture   Graduated

Graduate School 【 display / non-display

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    -
    1999.03

    University of Miyazaki  Graduate School, Division of Agriculture  Master's Course  Completed

  •  
    -
    2003.03

    Kagoshima University  Graduate School, Division of Agricltural Sciences  Doctor's Course  Accomplished credits for doctoral program

 

Papers 【 display / non-display

  • Field trial of insect-resistant and herbicide-tolerant genetically modified cotton (Gossypium hirsutum L.) for environmental risk assessment in Japan.

    Asanuma Y, Gondo T, Ishigaki G, Inoue K, Zaita N, Muguerza M, Akashi R

    GM crops & food   8   106 - 116   2017.04  [Refereed]

    Joint Work

    DOI PubMed

  • Sequencing and comparative analyses of the genomes of zoysiagrasses

    Tanaka H., Hirakawa H., Kosugi S., Nakayama S., Ono A., Watanabe A., Hashiguchi M., Gondo T., Ishigaki G., Muguerza M., Shimizu K., Sawamura N., Inoue T., Shigeki Y., Ohno N., Tabata S., Akashi R., Sato S.

    DNA Research   23   171 - 180   2016.04  [Refereed]

    Joint Work

     View Summary

    © The Author 2016. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. Zoysia is a warm-season turfgrass, which comprises 11 allotetraploid species (2n = 4x = 40), each possessing different morphological and physiological traits. To characterize the genetic systems of Zoysia plants and to analyse their structural and functional differences in individual species and accessions, we sequenced the genomes of Zoysia species using HiSeq and MiSeq platforms. As a reference sequence of Zoysia species, we generated a high-quality draft sequence of the genome of Z. japonica accession 'Nagirizaki' (334 Mb) in which 59,271 protein-coding genes were predicted. In parallel, draft genome sequences of Z. matrella 'Wakaba' and Z. pacifica 'Zanpa' were also generated for comparative analyses. To investigate the genetic diversity among the Zoysia species, genome sequence reads of three additional accessions, Z. japonica 'Kyoto', Z. japonica 'Miyagi' and Z. matrella 'Chiba Fair Green', were accumulated, and aligned against the reference genome of 'Nagirizaki' along with those from 'Wakaba' and 'Zanpa'. As a result, we detected 7,424,163 single-nucleotide polymorphisms and 852,488 short indels among these species. The information obtained in this study will be valuable for basic studies on zoysiagrass evolution and genetics as well as for the breeding of zoysiagrasses, and is made available in the 'Zoysia Genome Database' at http://zoysia.kazusa.or.jp.

    DOI PubMed

  • Arabidopsis galactinol synthase AtGolS2 improves drought tolerance in the monocot model Brachypodium distachyon

    Himuro Y., Ishiyama K., Mori F., Gondo T., Takahashi F., Shinozaki K., Kobayashi M., Akashi R.

    Journal of Plant Physiology   171   1127 - 1131   2014.08  [Refereed]

    Joint Work

     View Summary

    Brachypodium distachyon (purple false brome) is a herbaceous species belonging to the grass subfamily Pooideae, which also includes major crops like wheat, barley, oat and rye. The species has been established as experimental model organism for understanding and improving cereal crops and temperate grasses. The complete genome of Bd21, the community standard line of B. distachyon, has been sequenced and protocols for Agrobacterium-mediated transformation have been published. Further improvements to the experimental platform including better evaluation systems for transgenic plants are still needed. Here we describe the growth conditions for Bd21 plants yielding highly responsive immature embryos that can generate embryogenic calli for transformation. A prolonged 20-h photoperiod produced seeds with superior immature embryos. In addition, osmotic treatment of embryogenic calli enhanced the efficiency of transfection by particle bombardment. We generated transgenic plants expressing Arabidopsis thaliana galactinol synthase 2 (AtGolS2) in these experiments. AtGolS2-expressing transgenics displayed significantly improved drought tolerance, increasing with increased expression of AtGolS2. These results demonstrate that AtGolS2 can confer drought tolerance to monocots and confirm that Brachypodium is a useful model to further explore ways to understand and improve major monocot crop species. © 2014 Elsevier GmbH.

    DOI PubMed

  • Spontaneous appearance of polyploids in plants regenerated from embryogenic calli derived from seedling-meristems of ruzigrass (Brachiaria ruziziensis Germain et Everard)

    Ishigaki G., Gondo T., Rahman M., Umami N., Akashi R.

    Grassland Science   60   24 - 30   2014.03  [Refereed]

    Joint Work

     View Summary

    Ruzigrass (Brachiaria ruziziensis Germain et Everard cv. Kennedy) is an important forage grass in tropical and sub-tropical areas. Previously, we reported transgenic ruzigrass plants generated by our transformation system were sterile and tetraploid in spite of beginning with diploid plants. This study analyzed ploidy variation in embryogenic calli and the regenerants of diploid ruzigrass. The morphological traits and fertility were also investigated to develop a methodology for the production of stable transgenic lines. Embryogenic calli at different stages (2, 4, 6 and 12-month-old) were regenerated via somatic embryogenesis. An approach of flow cytometry (FCM) analysis was used to determine the ploidy level of embryogenic calli and regenerants of ruzigrass. FCM analysis revealed that embryogenic calli were spontaneously reduplicated at a high frequency and resulting regenerants were polyploids (tetraploid or octoploid), including 15 tetraploid regenerants (68%) and seven octoploid regenerants (32%) derived from 12-month-old embryogenic calli. These regenerants exhibited the morphological variations among different ploidy levels. The viability of pollen grains was significantly (P  <  0.01) decreased in tetraploid and octoploid regenerants. Our findings indicated that clarification and resolution of ploidy variation in ruzigrass combined with ploidy level checking using FCM analysis before transformation steps is crucial for plant regeneration in transformed ruzigrass. © 2014 Japanese Society of Grassland Science.

    DOI

  • Lignin content and digestibility in transgenic bahiagrass (Paspalum notatum Flügge) obtained by genetic manipulation of cinnamyl alcohol dehydrogenase gene

    Muguerza M., Gondo T., Ishigaki G., Akashi R.

    Asian Journal of Plant Sciences   13   8 - 17   2014  [Refereed]

    Joint Work

     View Summary

    Lignin is generally known as one of the major impediments for utilizing the potential of many forage plants because it limits digestibility and concomitantly, livestock productivity. Warm-season grasses yield high biomass but the digestibility is low due to their high lignin content. Cinnamyl alcohol dehydrogenase, a key enzyme that catalyzes the last stage in the lignin biosynthesis pathway, has been genetically altered in few grasses to increase their digestibility and forage quality. The aim of this study was to reduce the lignin content in a warm-season grass, bahiagrass (Paspalum notatum Flügge), by suppression of cinnamyl alcohol dehydrogenase gene expression. Using particle bombardment, cinnamyl alcohol dehydrogenase gene constructs with the antisense and RNAi vector under the control of the maize ubiquitin promoter were introduced into bahiagrass calli. The lignin content in most of the transgenic lines was significantly reduced, although the agronomic characteristics (plant height, leaf length, leaf width, tiller number, heading tiller and dry matter) differed between individuals. The in vitro dry matter digestibility of four of the nine transgenic plant significantly increased by 5.6-10.4% units. These results suggest that the molecular modification of the cinnamyl alcohol dehydrogenase gene function in the monolignol pathway significantly improved the feeding characteristics of the bahiagrass and that this approach could be used to improve the forage quality of other warm-season grasses. By utilizing their potential, novel cultivars could be developed that are amenable for intensified and sustainable grass forage production. © 2014 Asian Network for Scientific Information.

    DOI

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Review Papers 【 display / non-display