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Affiliation |
Faculty of Agriculture Department of Agricultural and Environmental Sciences |
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Title |
Associate Professor |
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External Link |
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INABA Takehito
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Degree 【 display / non-display 】
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博士（農学） ( 2002.3 名古屋大学 )
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修士（農学） ( 1999.3 名古屋大学 )
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学士（農学） ( 1997.3 名古屋大学 )
Research Areas 【 display / non-display 】
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Life Science / Applied molecular and cellular biology
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Life Science / Applied biochemistry
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Life Science / Plant nutrition and soil science
Papers 【 display / non-display 】
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Inhibition of in vitro pollen tube growth in ‘Hyuganatsu’ (Citrus tamurana hort. ex Tanaka) by recombinant S15-RNase protein Reviewed
Honsho, C., Inaba, T., Sugimoto, Y., Ishimura, S. and Tetsumura, T.
Acta Horticulturae in press 2024.2
Publishing type：Research paper (scientific journal)
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Tachibana R, Abe S, Marugami M, Yamagami A, Akema R, Ohashi T, Nishida K, Nosaki S, Miyakawa T, Tanokura M, Kim JM, Seki M, Inaba T, Matsui M, Ifuku K, Kushiro T, Asami T, Nakano T
Nature Communications 15 ( 1 ) 370 2024.1
Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Nature
Chloroplast development adapts to the environment for performing suitable photosynthesis. Brassinosteroids (BRs), plant steroid hormones, have crucial effects on not only plant growth but also chloroplast development. However, the detailed molecular mechanisms of BR signaling in chloroplast development remain unclear. Here, we identify a regulator of chloroplast development, BPG4, involved in light and BR signaling. BPG4 interacts with GOLDEN2-LIKE (GLK) transcription factors that promote the expression of photosynthesis-associated nuclear genes (PhANGs), and suppresses their activities, thereby causing a decrease in the amounts of chlorophylls and the size of light-harvesting complexes. BPG4 expression is induced by BR deficiency and light, and is regulated by the circadian rhythm. BPG4 deficiency causes increased reactive oxygen species (ROS) generation and damage to photosynthetic activity under excessive high-light conditions. Our findings suggest that BPG4 acts as a chloroplast homeostasis factor by fine-tuning the expression of PhANGs, optimizing chloroplast development, and avoiding ROS generation.
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Kitawaki K., Mihara R., Kamimura S., Sato A., Ushiyama M., Ito-Inaba Y., Inaba T.
Plant Physiology 193 ( 1 ) 234 - 245 2023.8
Language：English Publishing type：Research paper (scientific journal) Publisher：Plant physiology
The identification of chemical compounds that affect intracellular processes has greatly contributed to our understanding of plant growth and development. In most cases, these compounds have been identified in germinated seedlings. However, chemical screening using mature plants would benefit and advance our understanding of environmental responses. In this study, we developed a high-throughput screening method using single leaves of mature plants to identify small molecules that affect cold-regulated gene expression. A single excised leaf of Arabidopsis (Arabidopsis thaliana) grown in submerged cultures responded to low temperatures in terms of COLD-REGULATED (COR) gene expression. We used transgenic Arabidopsis harboring a COLD-REGULATED 15A (COR15A) promoter::luciferase (COR15Apro::LUC) construct to screen natural compounds that affect the cold induction of COR15Apro::LUC. This approach allowed us to identify derivatives of 1,4-naphthoquinone as specific inhibitors of COR gene expression. Moreover, 1,4-naphthoquinones appeared to inhibit the rapid induction of upstream C-REPEAT BINDING FACTOR (CBF) transcription factors upon exposure to low temperature, suggesting that 1,4-naphthoquinones alter upstream signaling processes. Our study offers a chemical screening scheme for identifying compounds that affect environmental responses in mature plants. This type of analysis is likely to reveal an unprecedented link between certain compounds and plant environmental responses.
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Koyamatsu D., Otsubo M., Ohira T., Sato M.P., Suzuki-Masuko H., Shiota T., Takenaka Takano K., Ozeki M., Otsuka K., Ogura Y., Hayashi T., Watanabe M., Inaba T., Ito-Inaba Y.
Physiologia Plantarum 175 ( 4 ) e13957 2023.7
Language：English Publishing type：Research paper (scientific journal) Publisher：Physiologia Plantarum
In floral thermogenesis, sugars play an important role not only as energy providers but also as growth and development facilitators. Yet, the mechanisms underlying sugar translocation and transport in thermogenic plants remain to be studied. Asian skunk cabbage (Symplocarpus renifolius) is a species that can produce durable and intense heat in its reproductive organ, the spadix. Significant morphological and developmental changes in the stamen are well-characterized in this plant. In this study, we focused on the sugar transporters (STPs), SrSTP1 and SrSTP14, whose genes were identified by RNA-seq as the upregulated STPs during thermogenesis. Real-time PCR confirmed that mRNA expression of both STP genes was increased from the pre-thermogenic to the thermogenic stage in the spadix, where it is predominantly expressed in the stamen. SrSTP1 and SrSTP14 complemented the growth defects of a hexose transporter-deficient yeast strain, EBY4000, on media containing 0.02, 0.2, and 2% (w/v) glucose and galactose. Using a recently developed transient expression system in skunk cabbage leaf protoplasts, we revealed that SrSTP1 and SrSTP14-GFP fusion proteins were mainly localized to the plasma membrane. To dig further into the functional analysis of SrSTPs, tissue-specific localization of SrSTPs was investigated by in situ hybridization. Using probes for SrSTP14, mRNA expression was observed in the microspores within the developing anther at the thermogenic female stage. These results indicate that SrSTP1 and SrSTP14 transport hexoses (e.g., glucose and galactose) at the plasma membrane and suggest that SrSTP14 may play a role in pollen development through the uptake of hexoses into pollen precursor cells.
DOI： 10.1111/ppl.13957
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Maekawa H, Otsubo M, Sato MP, Takahashi T, Mizoguchi K, Koyamatsu D, Inaba T, Ito-Inaba Y
Plant Cell Reports 41 ( 1 ) 263 - 275 2022.1
Language：English Publishing type：Research paper (scientific journal) Publisher：Plant Cell Reports
Key message: Floral thermogenesis is an important reproductive strategy for attracting pollinators. We developed essential biological tools for studying floral thermogenesis using two species of thermogenic aroids,Symplocarpus renifolius and Alocasia odora. Abstract: Aroids contain many species with intense heat-producing abilities in their inflorescences. Several genes have been proposed to be involved in thermogenesis of these species, but biological tools for gene functional analyses are lacking. In this study, we aimed to develop a protoplast-based transient expression (PTE) system for the study of thermogenic aroids. Initially, we focused on skunk cabbage (Symplocarpus renifolius) because of its ability to produce intense as well as durable heat. In this plant, leaf protoplasts were isolated from potted and shoot tip-cultured plants with high efficiency (ca. 1.0 × 105/g fresh weight), and more than half of these protoplasts were successfully transfected. Using this PTE system, we determined the protein localization of three mitochondrial energy-dissipating proteins, SrAOX, SrUCPA, and SrNDA1, fused to green fluorescent protein (GFP). These three GFP-fused proteins were localized in MitoTracker-stained mitochondria in leaf protoplasts, although the green fluorescent particles in protoplasts expressing SrUCPA-GFP were significantly enlarged. Finally, to assess whether the PTE system established in the leaves of S. renifolius is applicable for floral tissues of thermogenic aroids, inflorescences of S. renifolius and another thermogenic aroid (Alocasia odora) were used. Although protoplasts were successfully isolated from several tissues of the inflorescences, PTE systems worked well only for the protoplasts isolated from the female parts (slightly thermogenic or nonthermogenic) of A. odora inflorescences. Our developed system has a potential to be widely used in inflorescences as well as leaves in thermogenic aroids and therefore may be a useful biological tool for investigating floral thermogenesis.
Books 【 display / non-display 】
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温度と生命システムの相関学
中山克大, 大川久美子, 稲葉丈人ほか（ Role： Joint author）
東海大学出版会 2009.3
Language：Japanese Book type：General book, introductory book for general audience
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翻訳版 人工光合成 -生物学的基礎から工業技術的応用まで-
河野智謙・監訳（ Role： Joint translator）
エヌ・ティー・エス出版 2008.10
Language：Japanese Book type：Scholarly book
MISC 【 display / non-display 】
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Integration of cyanobacterial bicarbonate transporters into plant to improve photosynthesis Invited
2 ( 12 ) 82 - 86 2018.11
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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Energetic manipulation of chloroplast protein import and the use of chemical cross-linkers to map protein-protein interactions.
Inoue, H. Wang, F., Inaba, T. and Schnell, D.J.
Methods in Molecular Biology 774 2011.8
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Springer
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葉緑体機能の構築とプラスチドシグナル伝達経路.
稲葉丈人, 矢津芙美子, 柿崎智博
バイオサイエンスとインダストリー 68 394 - 397 2010.11
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution) Publisher：バイオインダストリー協会
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ストレス応答における親水性タンパク質の役割.
中山克大, 大川久美子, 稲葉丈人
化学と生物 46 128 - 134 2008.2
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution) Publisher：日本農芸化学会
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クラークと新島襄ゆかりの街、Amherstでの研究生活 - 葉緑体タンパク質輸送の一端を解き明かす -
稲葉丈人
化学と生物 44 491 - 495 2006.7
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution) Publisher：日本農芸化学会
Presentations 【 display / non-display 】
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Targeting of a polytopic membrane protein to the inner envelope membrane of chloroplasts involves multiple transmembrane segments.
Okawa, K., Inoue, H., Adachi, F., Nakayama, K., Ito-Inaba, Y., Schnell D.J., Uehara, S. and Inaba, T.
日本植物生理学会2014年度年会 (富山) 日本植物生理学会
Event date： 2014.3
Language：English Presentation type：Poster presentation
Venue：富山
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ザゼンソウの花成ホルモン(SrFT)が熱産生に及ぼす影響の検討
稲葉靖子, 増子(鈴木)潤美, 片山陽子, 渡辺正夫, 稲葉丈人
日本植物生理学会2014年度年会 (富山) 日本植物生理学会
Event date： 2014.3
Language：Japanese Presentation type：Poster presentation
Venue：富山
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植物細胞における葉緑体形成の分子機構 ～タンパク質輸送からシグナル伝達まで～
稲葉丈人
日本動物学会九州支部・九州沖縄植物学会・日本生態学会九州地区会学術講演会 (宮崎) 日本動物学会九州支部・九州沖縄植物学会・日本生態学会九州地区会
Event date： 2013.11
Language：Japanese Presentation type：Oral presentation (invited, special)
Venue：宮崎
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ポリトピック型膜タンパク質の葉緑体内包膜への輸送機構
稲葉丈人, 井上仁志, 大川久美子, 安達ふみ, 中山克大, 稲葉靖子, Danny J. Schnell
第37回蛋白質と酵素の構造と機能に関する九州シンポジウム (雲仙) 蛋白質と酵素の構造と機能に関する九州シンポジウム
Event date： 2013.9
Language：Japanese Presentation type：Poster presentation
Venue：雲仙
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プラスチドシグナル伝達因子GLK1の細胞内局在と組織特異的発現の解析
戸田真, 稲葉靖子, 矢津芙美子, 榊原陽一, 水光正仁, 稲葉丈人
平成24年度日本農芸化学会西日本支部大会 (鹿児島) 日本農芸化学会西日本支部
Event date： 2012.9
Language：Japanese Presentation type：Oral presentation (general)
Venue：鹿児島
Awards 【 display / non-display 】
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Excellent Scholar Award
2017.4 International Botanical Congress 2017
Takehito Inaba
Award type：Award from international society, conference, symposium, etc. Country：Japan
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宮崎大学農学部表彰論文 最優秀賞
2016.12 宮崎大学農学部
稲葉丈人
Country：Japan
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日本農芸化学会 農芸化学奨励賞
2009.3 日本農芸化学会
稲葉丈人
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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2008 Plant and Cell Physiology Cover Design Award 第3位
2009.3 Oxford University Press
Ito-Inaba, Y., Hida, Y., Mori, H. and Inaba, T.
Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
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日本農芸化学会東北支部奨励賞
2007.11 日本農芸化学会東北支部
稲葉丈人
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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ヒュウガナツ高精度参照ゲノム構築とそれを利用した枝変わり変異形質の原因遺伝子探索
Grant number：23H02205 2023.04 - 2027.03
日本学術振興会 科学研究費補助金 基盤研究(B)
Authorship：Coinvestigator(s)
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葉緑体電子伝達反応を利用した光合成発電デバイスの開発
Grant number：20K21282 2020.07 - 2023.03
独立行政法人日本学術振興会 科学研究費補助金 挑戦的研究（萌芽）
Authorship：Principal investigator
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カンキツ属植物の自家不和合性はS-RNaseに支配されているのか？
Grant number：19H02949 2019.04 - 2023.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
Authorship：Coinvestigator(s)
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合成生物学的手法を用いた高効率CO2流入経路の構築とそれに基づく光合成能の改良
Grant number：18H02169 2018 - 2022.03
科学研究費補助金 基盤研究(B)
Authorship：Principal investigator
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ハイブリッド型葉緑体を持つ植物の創出とその機能解析
2015.04 - 2018.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
ハイブリッド型葉緑体を持つ植物の創出とその機能解析