KONO Tomoya

写真a

Affiliation

Faculty of Agriculture Department of Biochemistry and Applied Biosciences

Title

Associate Professor

External Link

Degree 【 display / non-display

  • 博士(農学) ( 2003.3   鹿児島大学 )

Research Areas 【 display / non-display

  • Life Science / Aquatic bioproduction science

 

Papers 【 display / non-display

  • Comprehensive analysis of diel rhythmic expression of the medaka tlr gene family Reviewed

    Takahiko Hata, Suzuka Setoguchi, Natuki Morimoto, Hidetoshi Shimawaki, Jun-ichi Hikima, Masahiro Sakai, Tomoya Kono

    Developmental and Comparative Immunology   in press   2024

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

  • Susceptibility f Japanese Medaka to Edwardsiella piscicida and E. anguillarum isolated from aquacultured fish. Reviewed

    Aki Nishihara, Takechiyo Sumiyoshi, Koushirou Arakawa, Natsuki Morimoto, Shinya Yasumoto, Masakazu Kondo, Tomoya Kono, Masahiro Sakai, Jun-ichi Hikima

    Fish Pathology   58 ( 4 )   175 - 179   2023.12

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Interleukin-22 induces immune-related gene expression in the gills of Japanese medaka Oryzias latipes

    Watanabe M., Okamura Y., Kono T., Sakai M., Hikima J.i.

    Developmental and Comparative Immunology   148   2023.11

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    Publishing type:Research paper (scientific journal)   Publisher:Developmental and Comparative Immunology  

    The cytokine interleukin (IL)-22 has been identified in several fish species; however, its functional significance in the gills of these fish species remains unclear. In this study, we analyzed the expression of proinflammatory cytokines, antimicrobial peptides, and IL-22 binding protein in the gills of wild-type and IL-22-knockout (IL-22 KO) medaka under dextran sulfate sodium-induced inflammation. We also produced medaka recombinant IL-22 (rIL-22) and analyzed the expression of immune-related genes in rIL-22-stimulated primary cell cultures from gills. The il1b, il6, tnfa, and hamp genes were significantly upregulated in wild-type gills upon dextran sulfate sodium stimulation compared with the naïve state but not in IL-22 KO gills. il22bp transcripts were barely detectable in the IL-22 KO medaka gills. However, the expression of il1b, il6, hamp, and il22bp was upregulated in rIL-22-stimulated gill cell culture. These results suggest IL-22 could be involved in immune responses through inflammatory cytokine and antimicrobial peptide production in fish gills.

    DOI: 10.1016/j.dci.2023.104916

    Scopus

  • Transcriptomic analysis of cultured kuruma shrimp primary lymphoid organ cells for identifying key cascades hindering sustainable culture

    Tsuchiya A., Okamura Y., Kono T., Hikima J.i., Sakai M.

    Fisheries Science   89 ( 4 )   449 - 462   2023.7

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    Publishing type:Research paper (scientific journal)   Publisher:Fisheries Science  

    The progress of virology research in shrimp has been hampered by a lack of immortalized shrimp cell lines, and even long-term and stable primary cell cultures have not been perfectly achieved. Here, transcriptomic analysis of primary lymphoid cells in kuruma shrimp Marsupenaeus japonicus was performed to understand gene expression changes and stressors under existing culture conditions. In our optimized culture, the primary cells showed dead cell increases on days 3–4 and 6–7 after seeding. Next, we performed transcriptomic analysis at four time points (days 1, 3, 4, and 6 after tissue seeding), and RNA sequencing revealed that detected genes could be divided into three groups according to their expression changes: (1) downregulated on days 3–6, (2) upregulated on days 3–4, and (3) upregulated on day 6. Group 1 included platelet-derived growth factor receptor alpha and vascular endothelial growth factor 3 (MjVEGF3). Consistently, quantitative real-time polymerase chain reaction (qPCR) results showed a significant decrease in MjVEGF3 and its receptor (MjVEGFR) expression. These results suggest the importance of supplementation to recover VEGF signaling in the proper time-dependent manner for long and stable culture of shrimp lymphoid organ cells.

    DOI: 10.1007/s12562-023-01692-8

    Scopus

  • Evolutional perspective and functional characteristics of interleukin-17 in teleosts Reviewed

    Yo Okamura, Tomoya Kono, Masahiro Sakai (Review article)

    Fish and Shellfish Immunology   132   108496   2023.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Fish and Shellfish Immunology  

    Interleukin (IL)-17 is a proinflammatory cytokine and plays essential roles in adaptive and innate immune responses against bacterial and fungal infections. Especially in mammalian mucosal tissues, it is well known that innate immune responses via IL-17A and IL-17F, such as the production of antimicrobial peptides, are very important for microbiota control. In contrast, interesting insights into the functions of IL-17 have recently been reported in several teleost species, although little research has been conducted on teleost IL-17. In the present review, we focused on current insights on teleost IL-17 and speculated on the different or consensus parts of teleost IL-17 signaling compared to that of mammals. This review focuses on the role of teleost IL-17 in intestinal immunity. We expect that this review will encourage a further understanding of the roles and importance of IL-17 signaling in teleosts.

    DOI: 10.1016/j.fsi.2022.108496

    Scopus

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Books 【 display / non-display

  • 月刊「養殖」サイトカインを用いた魚類免疫賦活剤の効果判定

    酒井正博・高山博章・河野智哉( Role: Joint author)

    緑書房  2012.2 

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    Responsible for pages:54-57   Language:Japanese

MISC 【 display / non-display

  • Susceptibility of Japanese Medaka to <i>Edwardsiella piscicida</i> and <i>E. anguillarum</i> Isolated from Aquacultured Fish Reviewed

    Nishihara Aki, Sumiyoshi Takechiyo, Arakawa Koushiro, Morimoto Natsuki, Yasumoto Shinya, Kondo Masakazu, Kono Tomoya, Sakai Masahiro, Hikima Jun-ichi

    Fish Pathology   58 ( 4 )   175 - 179   2023

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    Language:English   Publishing type:Rapid communication, short report, research note, etc. (scientific journal)   Publisher:The Japanese Society of Fish Pathology  

    <i>Edwardsiella</i> bacteria infect Japanese medaka <i>Oryzias latipes</i>; however, the difference in susceptibility between <i>E. piscicida</i> and <i>E. anguillarum</i> is unknown. ​In this study, we performed challenge tests with medaka using four <i>Edwardsiella</i> strains, which were difficult to be identified by specific primers. ​The four strains were reclassified as three <i>E. piscicida</i> strains and one <i>E. anguillarum</i> strain using phylogenetic analysis. ​The immersion challenge demonstrated that medaka were more susceptible to <i>E. piscicida</i> than to <i>E. anguillarum</i>. ​Increases in the bacterial copy number in the infected medaka kidneys were correlated with the worsening of the external symptoms. ​Medaka is considered a valuable model organism for understanding the etiology of edwardsiellosis.

    DOI: 10.3147/JSFP.58.175

    Scopus

    CiNii Research

  • サイトカイン遺伝子を用いた魚類免疫賦活剤の効果判定

    酒井正博、高山博章、河野智哉

    養殖   2012.2

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)   Publisher:緑書房  

  • Genomics of fish IL-17 ligand and receptors: A review.

    T. Kono, H. Korenaga and M. Sakai

    Fish and Shellfish Immunology   ( 31 )   635 - 643   2010.12

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    Language:English   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Publisher:Elsevier  

Presentations 【 display / non-display

  • Edwardsiella piscicida由来ivyの宿主魚類に対する病原性

    西原輝、住吉竹千代、河野智哉、酒井正博、引間順一

    「集え、多分野研究者!」感染症キャンプin宮崎  2024.1.27 

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    Event date: 2024.1.26 - 2024.1.28

    Presentation type:Poster presentation  

  • Edwardsiella piscicida由来T6SS関連病原因子EvpPによる組織内増殖能および宿主免疫応答の阻害

    住吉竹千代、山本樹奈、出口華、西原輝、河野智哉、酒井正博、引間順一

    「集え、多分野研究者!」感染症キャンプin宮崎  2024.1.27 

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    Event date: 2024.1.26 - 2024.1.28

    Presentation type:Poster presentation  

  • 養殖メダカに発生した不明病の病原体検出

    平瀬楓香、川口航世、西原輝、楠ひかり、吉田恵史郎、加藤豪司、宮西弘、河野智哉、酒井正博、引間順一

    令和5年度日本魚病学会秋季大会  2023.9.3 

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    Event date: 2023.9.2 - 2023.9.3

    Presentation type:Oral presentation (general)  

  • Edwardsiella piscicida Ivyのリゾチーム阻害作用およびivy欠損株の宿主に対する感受性

    西原輝、住吉竹千代、河野智哉、酒井正博、引間順一

    第23回マリンバイオテクノロジー学会大会  2023.5.27 

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    Event date: 2023.5.27 - 2023.5.28

    Presentation type:Oral presentation (general)  

  • 炎症を誘導したIL-22遺伝子KOメダカの腸管における機能的変化

    高橋良枝、岡村洋、原田七輝、渡邊美香、宮西弘、河野智哉、酒井正博、引間順一

    令和3年度日本水産学会秋季大会 

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    Event date: 2021.9.13 - 2021.9.16

    Presentation type:Oral presentation (general)  

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Awards 【 display / non-display

  • 2022年 宮崎大学学生農学特別賞表

    2023.2   宮崎大学農学部   Diel rhythm of the inflammatory cytokine il1b in the Japanese medaka (Oryzias latipes) regulated by core components of the circadian clock

    Tomoya Takeuchi, Takahiko Hata, Hiroshi Miyanishi, Takumi Yuasa, Suzuka Setoguchi, Ayaka Takeda, Natsuki Morimoto, Jun-ichi Hikima, Masahiro Sakai and Tomoya Kono

  • 日本水産学会 水産学進歩賞

    2021.3   日本水産学会   魚類サイトカインによる自然免疫応答の概日リズム制御に関する研究

    河野智哉

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  • 宮崎大学農学部論文表彰

    2018.1   宮崎大学農学部   Identification and immunoregulatory function of neuromedin U (Nmu) in the Japanese pufferfish Takifugu rubripes

    河野智哉

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    Country:Japan

  • 北里大学同窓会研究奨励賞

    2012.9   北里大学   魚類生理活性ペプチドのクロストークによる免疫制御機構の解析

    河野智哉

  • 宮崎銀行ふるさと振興助成事業 学術研究部門

    2012.1   財団法人みやぎん経済研究所   宮崎県産養殖魚の健康を守る -生理活性ペプチドを用いた魚類の健康診断法の確立-

    河野智哉

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    Award type:Award from publisher, newspaper, foundation, etc. 

Grant-in-Aid for Scientific Research 【 display / non-display

  • 生物時計が調節する魚類の免疫リズムの解明 ~感染症の防除を目指して~

    Grant number:20H03064  2020.04 - 2024.03

    独立行政法人日本学術振興会  科学研究費補助金  基盤研究(B)

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    Authorship:Principal investigator 

  • フグはなぜ疾病に強いのか?フグの耐病性のメカニズムを探る

    2017.04 - 2021.03

    科学研究費補助金  基盤研究(A)

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    Authorship:Coinvestigator(s) 

  • 魚類の免疫応答における概日リズムの解明~免疫増強剤の効果向上を目指して~

    2017.04 - 2020.03

    科学研究費補助金  基盤研究(C)

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    Authorship:Principal investigator 

  • 魚類免疫細胞をマーカーとした疾病診断法の確立

    2014.04 - 2017.03

    科学研究費補助金  基盤研究(C)

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    Authorship:Principal investigator 

    魚類におけるヘルパーT(Th)細胞のサブセットを同定し、病原体感染時にこれらのThサブセットがどのような挙動を示すのかを解析し、新たな疾病診断技術の構築を目指すものである。

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