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Affiliation |
Faculty of Medicine College Hospital A |
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Title |
Assistant Professor |
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External Link |
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IKENOUE Makoto
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Papers 【 display / non-display 】
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Spatiotemporal expression of HMGB2 regulates cell proliferation and hepatocyte size during liver regeneration. Reviewed
Yano K, Choijookhuu N, Ikenoue M, Fukaya T, Sato K, Lee D, Taniguchi N, CHosa Em Nanashima A, Hisikawa Y
Scientific Reports 2022.7
Publishing type：Research paper (scientific journal)
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Knowledge survey regarding blast wound education of student doctors at a local academic medical university in Japan.
Kwano F, Munakata S, Tashiro K, Ikenoue M, Furukawa K, Ochiai H, Nakamura K, Nanashima A
Turkish Journal of Surgery 2022.5
Publishing type：Research paper (scientific journal)
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Yamaguma Y., Sugita N., Choijookhuu N., Yano K., Lee D., Ikenoue M., Fidya , Shirouzu S., Ishizuka T., Tanaka M., Yamashita Y., Chosa E., Taniguchi N., Hishikawa Y.
Histochemistry and Cell Biology 157 ( 3 ) 359 - 369 2022.3
Language：English Publishing type：Research paper (scientific journal) Publisher：Histochemistry and Cell Biology
High-mobility group box 2 (HMGB2) is a chromatin-associated protein that is an important regulator of gene transcription, recombination, and repair processes. The functional importance of HMGB2 has been reported in various organs, including the testis, heart, and cartilage. However, its role in the ovary is largely unknown. In this study, ovary tissues from wild-type (WT) and HMGB2-knock-out (KO) mice were examined by histopathological staining and immunohistochemistry. The ovary size and weight were significantly lower in HMGB2-KO mice than in age-matched WT littermates. Histopathological analysis revealed ovarian atrophy and progressive fibrosis in 10-month-old HMGB2-KO mouse ovaries. Compared to age-matched WT mice, the numbers of oocytes and developing follicles were significantly decreased at 2 months of age and were completely depleted at 10 months of age in HMGB2-KO mice. Immunohistochemistry revealed the expression of HMGB2 in the granulosa cells of developing follicles, oocytes, some corpora lutea, and stromal cells. Importantly, HMGB2-positive cells were co-localized with estrogen receptor beta (ERβ), but not ERα. Estrogen response element-binding activity was demonstrated by southwestern histochemistry, and it was decreased in HMGB2-KO mouse ovaries. Cell proliferation activity was also decreased in HMGB2-KO mouse ovaries in parallel with the decreased folliculogenesis. These results indicated that the depletion of HMGB2 induced ovarian atrophy that was characterized by a decreased ovarian size and weight, progressive fibrosis, as well as decreased oocytes and folliculogenesis. In conclusion, we demonstrated the crucial role of HMGB2 in mouse ovarian folliculogenesis through ERβ expression.
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Sugita N, Choijookhuu N, Yano K, Lee D, Ikenoue M, Fidya, Taniguchi N, Chosa E, Hishikawa Y
Biology of Reproduction 105 ( 6 ) 1510 - 1520 2021.10
Language：English Publishing type：Research paper (scientific journal) Publisher：Biology of reproduction
High-mobility group box 2, a chromatin-associated protein that interacts with deoxyribonucleic acid, is implicated in multiple biological processes, including gene transcription, replication, and repair. High-mobility group box 2 is expressed in several tissues, including the testis; however, its functional role is largely unknown. Here, we elucidated the role of high-mobility group box 2 in spermatogenesis. Paraffin-embedded testicular tissues were obtained from 8-week-old and 1-year-old wild-type and knock-out mice. Testis weight and number of seminiferous tubules were decreased, whereas atrophic tubules were increased in high-mobility group box 2-depleted mice. Immunohistochemistry revealed that atrophic tubules contained Sertoli cells, but not germ cells. Moreover, decreased cell proliferation and increased apoptosis were demonstrated in high-mobility group box 2-depleted mouse testis. To elucidate the cause of tubule atrophy, we examined the expression of androgen and estrogen receptors, and the results indicated aberrant expression of androgen receptor and estrogen receptor alpha in Sertoli and Leydig cells. Southwestern histochemistry detected decreased estrogen response element-binding sites in high-mobility group box 2-depleted mouse testis. High-mobility group box 1, which has highly similar structure and function as high-mobility group box 2, was examined by immunohistochemistry and western blotting, which indicated increased expression in testis. These findings indicate a compensatory increase in high-mobility group box 1 expression in high-mobility group box 2 knock-out mouse testis. In summary, depletion of high-mobility group box 2 induced aberrant expression of androgen receptor and estrogen receptor alpha, leading to decreased germ cell proliferation and increased apoptosis which resulted in focal seminiferous tubule atrophy.
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Kawano F., Tashiro K., Ikenoue M., Munakata S., Nakao H., Mizuno T., Mori H., Ikeda T., Takeno S., Furukawa K., Tomita M., Endo G., Ochiai H., Nakamura K., Nanashima A.
Surgery Today 51 ( 6 ) 1001 - 1009 2021.6
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Surgery Today
Purposes: Balancing scheduled surgery and trauma surgery is difficult with a limited number of surgeons. To address the issues and systematize education, we analyzed the current situation and the effectiveness of having a trauma team in the ER of a regional hospital. Methods: This retrospective study analyzed the demographics, traumatic variables, procedures, postoperative morbidities, and outcomes of 110 patients who underwent trauma surgery between 2012 and 2019. The trauma team was established in 2016 and our university hospital Emergency Room (ER) opened in 2012. Results: Blunt trauma accounted for 82% of the trauma injuries and 39% of trauma victims were transported from local centers to our institute. The most frequently injured organs were in the digestive tract and about half of the interventions were for hemostatic surgery alone. Concomitant treatments for multiple organ injuries were performed in 31% of the patients. The rates of postoperative severe complications (over Clavien–Dindo IIIb) and mortality were 10% and 13%, respectively. Fourteen (12.7%) of 24 patients who underwent damage-control surgery died, with multiple organ injury being the predominant cause of death. Conclusion: Systematic education or training of medical students and general surgeons, as well as the co-operation of the team at the regional academic institute, are necessary to overcome the limited human resources and save trauma patients.
Grant-in-Aid for Scientific Research 【 display / non-display 】
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脂肪肝マウスの肝再生過程でのミトコンドリア脂質代謝へのエストロゲンの関与
Grant number：21K06738 2021.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
菱川 善隆,
Authorship：Coinvestigator(s)