田岡 洋介 (タオカ ヨウスケ)

TAOKA Yosuke










出身大学院 【 表示 / 非表示


    鹿児島大学大学院  連合農学研究科  博士課程  単位取得満期退学

取得学位 【 表示 / 非表示

  • 鹿児島大学 -  博士(水産学)

専門分野(科研費分類) 【 表示 / 非表示

  • 応用微生物学

  • 水圏生産科学


論文 【 表示 / 非表示

  • Production of anserine-rich fish sauce from giant masu salmon, Oncorhynchus masou masou and γ-aminobutyric acid (GABA)-enrichment by Lactobacillus plantarum strain N10

    Taoka Y., Nakamura M., Nagai S., Nagasaka N., Tanaka R., Uchida K.

    Fermentation   5 ( 2 )   2019年06月  [査読有り]



    © 2019 by the authors. Previously, we developed a novel production technique for giant masu salmon (GMS). This study aimed to develop a fish sauce from GMS to explore ways to efficiently utilize the salmon and to enrich the fish sauce with γ-aminobutyric acid (GABA) by microbial fermentation. The minced bodies of GMS were autolyzed by endogenous protease at 55 °C and 60 °C. During autolysis, the changes in total free amino acids and protein size was monitored by LC-MS and SDS-PAGE analysis, respectively. After 96 h, fish sauce was prepared by heating, and the amino acid composition was analyzed by LC-MS. To enrich the fish sauce with GABA, Lactobacillus plantarum strain N10 was added and incubated at 28 °C for 48 h. The total free amino acids content significantly increased for 96 h. SDS-PAGE analysis showed that major bands at 200 kDa and 48 kDa detected at 0 h gradually disappeared over time. The ratio of anserine to total amino acids in the fish sauce was approximately 36%. The concentration of GABA in the fish sauce significantly increased through the addition of strain N10. Thus, anserine-rich fish sauce could be quickly produced from GMS, and the fish sauce was enriched with GABA by microbial fermentation.


  • Effect of Lactococcus lactis K-C2 on the growth performance, amino acid content and gut microflora of amberjack Seriola dumerili

    Linh N., Nagai S., Nagasaka N., Okane S., Taoka Y.

    Fisheries Science   84 ( 6 ) 1051 - 1062   2018年11月  [査読有り]



    © 2018, Japanese Society of Fisheries Science. This study aimed to evaluate the effect of Lactococcus lactis K-C2 on the growth performance, microbial diversity, and release of free amino acids in the intestinal tract and the edible parts of young amberjack, Seriola dumerili. Fish were fed a diet with or without strain K-C2 (2 × 1010 cfu/g feed) for 25 days. The results indicated that the growth performance of fish in the treated group was significantly higher than those in the control group (p < 0.05). The amount of five amino acids (aspartate, sarcosine, taurine, alanine, and arginine) in the gut content and 13 of 21 amino acids in the edible parts of fish in the treated group were significantly higher (p < 0.05) than those in the control group. Sphingomonas, Propionibacterium, and Mycobacterium were observed in gut microflora of fish in both the control and treated groups. Staphylococcus and Kocuria were detected in one sample from the control and treated groups; Acinetobacter and Acidobacteria were found in one sample from the control group. L. lactis was only found in one sample in the treated group. In conclusion, the dietary administration of probiotic L. lactis stimulated growth, reduced feed consumption, and improved the nutritional value of cultured amberjack.


  • Effect of seawater reared on the nutritional composition and antioxidant activity of edible muscle in smoltified-landlocked masu salmon (Oncorhynchus masou masou)

    Tanaka R., Uchida K., Ishimaru M., Ito M., Matsumoto N., Taoka Y., Hatate H.

    Journal of Food Measurement and Characterization   12 ( 1 ) 200 - 208   2018年03月  [査読有り]



    © 2017, Springer Science+Business Media, LLC. A new system of aquaculture for landlocked masu salmon (Oncorhynchus masou masou) has recently been developed in the southern Kyushu Island, Miyazaki, Japan. In this system, moderately silvering fish (smolt salmon) are artificially transported from the freshwater aquaculture ponds to floating seawater fish preserve, and they are then aquacultured during winter season, starting from December to the following April. As a result, their growth and body mass were greatly increased (total length: 21.25 ± 1.04–37.80 ± 3.71 cm; body weight: 81.75 ± 19.91–632.00 ± 172.42 g; condition factor: 8.45 ± 1.44–11.4 ± 0.74). In addition, There was a marked increase in chemical components such as the lipid contents (35.0 ± 9.4–79.2 ± 6.7 mg/g muscle), α-tocopherol (29.0 ± 17.0–178.6 ± 15.0 μg/g muscle), astaxanthin (0.1 ± 0.1–15.2 ± 8.8 μg/g muscle), and anserine (4.75 ± 0.59–10.32 ± 2.68 mg/g muscle) in the seawater-reared landlocked masu salmon. Furthermore, the antioxidant activity of the water soluble extract from the seawater-reared landlocked masu salmon increased from 480.96 ± 79.295–848.99 ± 182.79 μmol Trolox equivalent/g tissue. This new system that involves a short period of aquaculture in seawater not only promotes the growth of landlocked masu salmon, but may also improve its nutritional components and antioxidant activity.


  • Screening of lactic acid bacteria isolated from fermented food as potential probiotics for aquacultured carp and amberjack

    Linh N., Sakai K., Taoka Y.

    Fisheries Science   84 ( 1 ) 101 - 111   2018年01月  [査読有り]



    © 2017, Japanese Society of Fisheries Science. This study aimed to isolate lactic acid bacteria from fermented foods and evaluate their probiotic properties for application to aquaculture. Sixty-five bacteria strains were isolated using MRS (de Man, Rogosa & Sharpe) media. Using a double-layer agar method, three strains, GYP 31, L 15 and K-C2, showed antagonistic activities against all test pathogens belonging to Edwardsiella tarda, Streptococcus dysgalactiae, S. iniae and Lactococcus garvieae. These strains were able to survive in a pH range from 2.0 to 9.0 and at NaCl concentrations of 0, 3 and 5%. In the tolerance test, strain K-C2 displayed higher tolerance than strains GYP 31 and L 15 under the stimulation of acidic pH buffers and artificial gastric-intestinal juices. Thus, strain K-C2 was selected as a probiotic candidate and identified as L. lactis based on the sequences determined in 16S rRNA gene (1438 bp) analysis. It was observed to be ovoid in shape and to be catalase-negative in morphological and biochemical investigations. Strain K-C2 adhered to carp and amberjack intestinal mucus at bacterial densities of 1010cfu/ml in in vitro tests with adhesion rates of 62 and 58%, respectively. Thus, L. lactis strain K-C2 was considered to be a potential probiotic candidate for use in sustainable aquaculture.


  • Degradation of distillery lees (Shochu kasu) by cellulase-producing thraustochytrids

    Taoka Y., Nagano N., Kai H., Hayashi M.

    Journal of Oleo Science   66 ( 1 ) 31 - 40   2017年



    © 2017 by Japan Oil Chemists’ Society. Single cell oils produced by oleaginous microorganisms have attracted increasing interests as a petroleum alternative energy. Marine eukaryotes, thraustochytrids were heterotrophic, and can grow rapidly and accumulate large amount of lipids containing functional fatty acids, such as docosahexaenoic acid (DHA) in their cells body. In this investigation, thraustochytrids isolated from marine environment were cultured in the medium containing an industrial waste and an unused resource, distillery lees (Shochu kasu) to produce biofuel or functional fatty acids by microorganisms. Sixty-nine thraustochytrids and Schizochytrium aggregatum ATCC 28209 were screened for cellulase production, and the activities were detected using sodium carboxymethyl cellulose (CMC) as a substrate. Based on the screening test, strain TM02Bc identified to Schizochytrium sp. was selected for the Shochu kasu degradation test and compared with S. aggregatum ATCC 28209 previously known as a cellulase-producing thraustochytrid. Strains TM02Bc and ATCC 28209 were cultured in artificial seawater containing Shochu kasu for 15 days. The two strains could degrade Schochu kasu, especially that from sweet potato Shochu (Imo Shochu). Cellulase (CMCase) and protease activities were detected in culture supernatant of both strains, and the ratio of polyunsaturated fatty acids (PUFAs) significantly increased as a result of incubation of Shochu kasu with two strains. This preliminary study indicated that strain TM02Bc was a potent candidate for Shochu kasu treatment and fatty acid production.

    DOI CiNii

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総説・解説記事 【 表示 / 非表示

  • Effects of cold shock treatment on total lipid content and fatty acid composition of Aurantiochytrium limacinum strain mh0186

    Taoka Y., Nagano N., Okita Y., Izumida H., Sugimoto S., Hayashi M.

    Journal of Oleo Science ( Journal of Oleo Science )  60 ( 5 ) 217 - 220   2011年

    総説・解説(大学・研究所紀要)   共著


    To examine the effect of cold shock treatment on the fatty acid composition of Aurantiochytrium limacinum strain mh0186, a marine thraustochytrid, we cultivated this strain at 28°C for 72 h with shaking and stored the obtained biomass at 10°C for 72 h. A growth experiment was carried out for comparison, wherein strain mh0186 was grown at 10 and 15°C for 72 h with shaking, and it was found that the unsaturation of fatty acids was accelerated relative to that at 28°C. In the cold shock experiment, the total lipid content signifi cantly increased during storage at 10°C for 72 h. Overall, the percentage of unsaturated fatty acids such as docosahexaenoic acid was almost stable while that of n-6 docosapentaenoic acid decreased slightly, but signifi cantly, relative to that in the growth experiment. © 2011 by Japan Oil Chemists' Society.

    DOI CiNii

工業所有権 【 表示 / 非表示

  • ヤブレツボカビ類を用いたタンナーゼ活性を有するタンパク質の製造方法

    特願 2015-003492 


  • ヤブレツボカビ類を用いたリグニン分解活性を有するタンパク質の製造方法

    特願 2015-003488  特許 特許第6682109号


  • ヤブレツボカビ類を用いたカタラーゼ活性を有するタンパク質の製造方法

    特願 2014-023848  特開 2015-149912 


  • 酵素の製造方法

    特願 2007-201014  特開 2009-34037 


その他研究活動 【 表示 / 非表示

  • 平成30年度拡充(ソフト)事業"細島いわがき"成分検査事業





  • TECH PLANTER 2018 宮崎大会



  • 宮崎県日向市沿岸の水質調査とブランド化に関わる研究





  • 宮崎県高鍋町沿岸海水質調査





  • 養殖の餌と水





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学術関係受賞 【 表示 / 非表示

  • 平成23年度日本水産学会論文賞

    2012年03月28日   公益社団法人日本水産学会  

    受賞者:  田岡洋介、前田広人、Jae-Yoon JO、Min-Jee JEON、Sungchul C. BAI、Won-Jae LEE、弓削寿哉、越塩俊介

  • 平成18年度日本防菌防黴学会論文賞

    2006年05月30日   鹿児島大学  

    受賞者:  塩崎靖志、前田広人、田岡洋介、越塩俊介、吉川毅、坂田泰造、C. Saclauso

その他競争的資金獲得実績 【 表示 / 非表示

  • ラビリンチュラを利用したハマグリの高付加価値化

    提供機関:  宮崎大学  みやざき地域志向教育研究経費

    研究期間: 2016年07月  -  継続中  代表者:  田岡洋介



  • 県産食材を活かしたロコモ食の開発

    提供機関:  文部科学省  「ロコモティブシンドローム(ロコモ)の病態解明・対策―地方創生・ロコモザワールド宮崎の構築―」に関する事業

    研究期間: 2016年04月  -  継続中  代表者:  田岡洋介



  • GABA高生産乳酸菌を用いたGABA強化糠床及び、GABA強化漬物作製のための漬物容器の開発

    提供機関:  総務省  産学官共同研究開発支援事業(宮崎県単事業)

    研究期間: 2015年10月  -  継続中 



  • ロコモ滞在型リゾートの拡充に関する研究

    提供機関:  地方自治体  宮崎市地域貢献学術助成金

    研究期間: 2015年07月  -  2016年03月 



  • 日向市沿岸の水質調査及び水産資源の利用とブランド化推進に 関する研究

    提供機関:  総務省  みやざき地域志向教育研究経費

    研究期間: 2015年07月  -  2016年03月 



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研究発表 【 表示 / 非表示

  • 宮崎県日向市沿岸における養殖牡蠣の成長、グリコーゲン、ミネラル 及びアミノ酸組成の季節的変動

    小原有紀,濵㟢菜々子,松本明子,境健太郎, 黒木信介,田岡洋介

    平成31年度日本水産学会春季大会  (東京海洋大学)  2019年03月  -  2019年03月    日本水産学会

  • Comprehensive Production and Application Technology of both Lipid and Protein Derived from Eukaryotic microbes, Thraustochytrids

    Yousuke Taoka, Noriyuki Tai  [招待有り]

    [IMT-GT 2018  (Penang, Malaysia)  2018年12月  -  2018年12月    IMT-GT

  • Development of Fishmeal-Free Aquafeeds with Thraustochytrids-Protein

    Noriyuki Tai, Kenya Horii and Yousuke Taoka  [招待有り]

    BIT’s 8th Annual World Congress of Aquaculture and Fisheries-2018  (Weihai, China)  2018年10月  -  2018年10月    BIT'S



  • Production of Anserine-Rich Fish Sauce from Giant Masu Salmon, Oncorhynchus masou masou

    Yousuke Taoka

    BIT’s 6th Annual World Congress of Aquaculture and Fisheries-2017  (Shenzhen, China)  2017年11月  -  2017年11月   


    Recently, Dr. Katsuhisa Uchida who belong to University of Miyazaki, Japan, succeeded in establishing a production technique for giant masu salmon by transferring landlocked yamame to the sea for rearing during the winter season. The production rate and yield of the fish-mass significantly increased by culturing in seawater. The purpose of this study was to produce a fish sauce from newly developed fishery source, giant mass salmon by rapid fermentation under no-salt conditions. The whole body of giant masu salmon except for eggs was minced well, and the activity of protease contained in the mixtures was analyzed at 55oC and 60oC according to the adequate temperature indicated by our previous study. The protease activity was comparable to our previous study, and the activity at 55 and 60oC was 33 and 49 Unit/ g-protein, respectively. Giant masu salmon mixtures were incubated to induce autolysis at 55 or 60oC for 72 hrs. During incubation, to evaluate the degree of autolysis, the amino acid composition was analyzed with liquid chromatograph-mass spectrometry (LC-MS). The distribution of protein molecular size was analyzed by SDS-PAGE. The content of total amino acids was increased as time passed, and the low molecular weight bands appeared by incubation. Giant masu salmon mixtures were incubated at 60oC for 24 h, and the centrifugal supernatant was heated in boiling water for 20 min. The supernatant of the heated solution was used as giant masu salmon fish sauce (GMSFS). The ratio of anserine which well known as a functional amino acid, in GMSFS was significantly high (≒48% to total amino acid). Thus, the anserine-rich fish sauce could be produced quickly from giant masu salmon. At present, to add more functionality to GMSFA and modify the taste, we are carrying out the fermentation trial of GMSFS with lactic acid bacteria after autolysis.

  • Rearing Trial of Eel, Anguilla japonica in a Closed Recirculating System Using Hot Spring Water Welled up in Saito City, Miyazaki, Japan

    Seika Okane, Kaho Umezu, Mitsuki Horoiwa, Yukie Kamata, Yuki Hayami1, Setsuko Nagai1, Noriko Nagasaka1, Kenya Horii, Nguyen Thi Hue Linh, Ryusuke Tanaka, Katsuhisa Uchida Yousuke Taoka

    International Symposium Fisheries Science for Future Generation  (東京海洋大学)  2017年09月  -  2017年09月    日本水産学会

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共同研究希望テーマ 【 表示 / 非表示

  • プロバイオティクスを用いた水産増養殖における魚病予防技術の確立

  • 陸上養殖技術への微生物の応用

  • 発酵微生物を用いた新規発酵食品の開発

  • 油糧微生物を用いたバイオ燃料生産に関する研究


提供可能な資源 【 表示 / 非表示

  • みやざきサクラマス加工残渣を用いた魚醤


  • 油糧微生物ラビリンチュラ分離株


  • 水産養殖用プロバイオティクス