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Affiliation |
Faculty of Agriculture Department of Biochemistry and Applied Biosciences |
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Title |
Associate Professor |
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External Link |
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HIRANO Tomonari
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Degree 【 display / non-display 】
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Doctor (Agriculture) ( 2006.3 Chiba University )
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修士（農学） ( 2003.3 千葉大学 )
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学士（農学） ( 2001.3 千葉大学 )
Research Interests 【 display / non-display 】
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Mutation
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DNA double strand break
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Flower development
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Double fertilization
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Male gamete
Research Areas 【 display / non-display 】
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Environmental Science/Agriculture Science / Horticultural science
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Environmental Science/Agriculture Science / Science in plant genetics and breeding
Papers 【 display / non-display 】
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Genomic view of heavy-ion-induced deletions associated with distribution of essential genes in Arabidopsis thaliana Reviewed International journal
Kotaro Ishii , Yusuke Kazama, Tomonari Hirano, Jeffrey A. Fawcett, Muneo Sato, Masami Yokota Hirai, Fujiko Sakai, Yuki Shirakawa, Sumie Ohbu, Tomoko Abe
Frontiers in Plant Science 15 1352564 2024.4
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Tomonari Hirano, Muneaki Murata, Yurie Watarikawa, Yoichiro Hoshino, Tomoko Abe, Hisato Kunitake
Plant Reproduction 2024.4
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Plant Reproduction
Key message: In Cyrtanthus mackenii, development of embryo and endosperm were differentially affected by fertilization of male gametes with DNA damage and mutations. Abstract: Pollen irradiation with ionizing radiations has been applied in plant breeding and genetic research, and haploid plant induction has mainly been performed by male inactivation with high-dose irradiation. However, the fertilization process of irradiated male gametes and the early development of embryo and endosperm have not received much attention. Heavy-ion beams, a type of radiation, have been widely applied as effective mutagens for plants and show a high mutation rate even at low-dose irradiation. In this study, we analyzed the effects of male gametes of Cyrtanthus mackenii irradiated with a carbon-ion beam at low doses on fertilization. In immature seeds derived from the pollination of irradiated pollen grains, two types of embryo sacs were observed: embryo sac with a normally developed embryo and endosperm and embryo sac with an egg cell or an undivided zygote and an endosperm. Abnormalities in chromosome segregation, such as chromosomal bridges, were observed only in the endosperm nuclei, irrespective of the presence or absence of embryogenesis. Therefore, in Cyrtanthus, embryogenesis is strongly affected by DNA damage or mutations in male gametes. Moreover, various DNA contents were detected in the embryo and endosperm nuclei, and endoreduplication may have occurred in the endosperm nuclei. As carbon-ion irradiation causes chromosomal rearrangements even at low doses, pollen irradiation can be an interesting tool for studying double fertilization and mutation heritability.
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Characterization of a novel mutant with inhibition of storage root formation in sweet potato Reviewed
Hyungjun Park, Tomoko Abe, Hisato Kunitake, Tomonari Hirano
Breeding Science 73 ( 2 ) 212 - 218 2023.6
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：日本育種学会
Sweet potato is a widely cultivated crop with storage roots. Although many studies have been conducted on the mechanism of its storage root formation, the details have not been fully elucidated. We screened mutant lines with inhibition of storage root formation to clarify parts of the mechanism. In this study, the process of storage root formation in one of the mutant lines, C20-8-1, was investigated. The inhibition of storage root formation was observed during the early stages of growth. The roots in C20-8-1 did not show histological differences compared to those in wild type. The transition from fibrous roots to pencil roots, which are the developmental stages prior to mature storage root formation, was delayed or inhibited in C20-8-1. The upregulation of starch biosynthesis-related genes and downregulation of lignin biosynthesis genes with storage root swelling were not confirmed in the root of C20-8-1 during the developmental transition stage, suggesting that most of the roots in C20-8-1 are in the pre-transition state toward the storage root swelling. C20-8-1 showed a mutant phenotype during the critical period of storage root swelling initiation, and further clarification of this mutation is expected to provide new insights into storage root formation.
DOI： 10.1270/jsbbs.22090
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Effect of Growth Stages on Anthocyanins and Polyphenols in the Root System of Sweet Potato. Reviewed
Setoguchi Y, Nakagawa S, Ohmura R, Toshima S, Park H, Narasako Y, Hirano T, Otani M, Kunitake H
Plants (Basel, Switzerland) 12 ( 9 ) 2023.5
Language：English Publishing type：Research paper (scientific journal)
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Amphidiploid production of hybrid between black raspberry and Rubus parvifolius L., a wild Asian species, using colchicine treatment Reviewed
Saki Toshima, Itsuki Katsumi, Arisa Kai, Masaki Yahata, Tomonari Hirano, Hisato Kunitake
Scientia Horticulturae 312 111863 2023.3
Language：English Publishing type：Research paper (scientific journal)
Books 【 display / non-display 】
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國武 久登 , 執行 正義, 平野 智也, 神戸 敏成, 貴島 祐治 , 三吉 一光, 大谷 基泰, 八幡 昌紀, 星野 洋一郎, 中野 優 , 佐々 英徳, 三柴 啓一郎, 山本 伸一 (農学)（ Role： Joint editor）
講談社 2023.5 （ ISBN:9784065308158 ）
Language：Japanese Book type：Textbook, survey, introduction
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最新農業技術 花卉〈vol.5〉切り花で新境地をひらくアジサイ、ダリア、ラナンキュラス、ユーストマ
阿部 知子， 平野 智也（ Role： Joint author , 突然変異（イオンビーム）育種）
農山漁村文化協会 2013.3
Language：Japanese Book type：Scholarly book
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Pollen: Structure, Types and Effects
Tomonari Hirano, Yoichiro Hoshino（ Role： Joint author , Capture of male gamete dynamics in pollen tubes）
Nova Science Publishers 2010.7
Language：English Book type：Scholarly book
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日本の植物園における生物多様性保全
平野 智也， 神戸 敏成（ Role： Joint author , 日本産希少植物の繁殖および長期保存）
日本植物園協会・国立博物館筑波実験植物園・植物園自然保護国際機構 2007.3
Language：Japanese Book type：Scholarly book
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Floriculture, Ornamental and Plant Biotechnology: advances and topical issues
Tomonari Hirano, Keiko Ishikawa, Masahiro Mii（ Role： Joint author , Advances in Orchid Cryopreservation）
Global Science Book 2006.6
Language：English Book type：Scholarly book
MISC 【 display / non-display 】
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Development of breeding technology by using heavy-ion beam Invited Reviewed
Ion-Beam Breeding Research Group, Abe Tomoko, Kazama Yusuke, Hirano Tomonari
Breeding Research 25 ( 2 ) 170 - 176 2023.12
Authorship：Last author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Japanese Society of Breeding
DOI： 10.1270/jsbbr.23j13
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イオンビームを用いた花き植物の品種改良 Invited Reviewed
平野智也
アグリバイオ 6 ( 12 ) 14 - 18 2022.10
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
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Mutagenic Effects of Heavy-Ion Beam Irradiation to Plant Genome Reviewed
Tomonari Hirano, Yusuke Kazama, Hisato Kunitake, Tomoko Abe
CYTOLOGIA 87 ( 1 ) 3 - 6 2022.3
Authorship：Lead author, Corresponding author Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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Chromosomal rearrangement: From induction by heavy-ion irradiation to in vivo engineering by genome editing Reviewed
Yusuke Kazama, Tomonari Hirano, Tomoko Abe, Sachihiro Matsunaga
Cytologia 83 ( 2 ) 125 - 128 2018.6
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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重イオンビームで広がる花きの新品種作出 Invited Reviewed
平野 智也，市田 裕之，阿部 知子
化学と生物 55 ( 11 ) 775 - 782 2017.10
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
Presentations 【 display / non-display 】
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サツマイモ基腐病抵抗性品種選抜のためのポリフェノールマーカーの開発
瀬戸口優乃、福留春香、白石麗依奈、奈良迫洋介、竹下稔、 大谷基泰、平野智也、國武久登
日本作物学会第257回講演会 2024.3.28
Event date： 2024.3.28 - 2024.3.29
Language：Japanese Presentation type：Poster presentation
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オオカナダモの異型細胞形成の研究：細胞小器官の構造について
藤原誠、小林永実、河野杏奈、金澤美加子、平野智也、阿部知子、伊藤竜一
日本生物教育学会第108回全国大会
Event date： 2024.1.6 - 2024.1.8
Language：Japanese Presentation type：Oral presentation (general)
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Phylogenetic classification of a Japanese raspberry wild species, Rubus parvifolius International conference
Saki Toshima, Yuichi Saeki, Tomonari Hirano, Hisato Kunitake
The 4th Asian Horticultural Congress (AHC2023) 2023.9.29
Event date： 2023.9.29 - 2023.9.30
Language：English Presentation type：Oral presentation (general)
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大学発サツマイモ育種の試み
瀬戸口優乃、奈良迫洋介、大谷基泰、平野智也、國武久登
第23回 分析講演会 2023.9.22
Event date： 2023.9.22
Language：Japanese Presentation type：Poster presentation
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Effects of fertilization of male gametes with argon-ion irradiation on embryo and endosperm development in Cyrtanthus mackenii International conference
Makiko Shii, Yuki Kajiya Tomoko Abe, Hisato Kunitake, Tomonari Hirano
15th JKTC International Student Seminar 2023.9.19
Event date： 2023.9.18 - 2023.9.21
Language：English Presentation type：Oral presentation (general)
Awards 【 display / non-display 】
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日本育種学会賞
2023.3 日本育種学会 重イオンビームによる育種技術の開発
重イオン育種技術開発グループ（阿部知子、風間裕介、平野智也）
Award type：Award from Japanese society, conference, symposium, etc.
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平成28年度園芸学会年間優秀論文賞
2017.3 園芸学会
澤田 倫平, 平野 智也, 飯牟禮 和彦, 阿部 知子, 尾崎 行生
Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
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RIKEN Accelerator Progress Report 2015 Vol. 49 HIGHLIGHTS OF THE YEAR
2017.3 仁科加速器研究センター加速器年次報告編集委員会
A. Hisamura, D. Mine, T. Takeda, T. Abe, Y. Hayashi, T. Hirano
Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
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RIKEN Accelerator Progress Report 2015 Vol. 49 HIGHLIGHTS OF THE YEAR
2017.3 仁科加速器研究センター加速器年次報告編集委員会
T. Hirano, Y. Kazama, K. Ishii, S. Ohbu, Y. Shirakawa, T. Abe
Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
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第125回講演会日本育種学会優秀発表賞 (講演番号P28)
2014.5 日本育種学会
平野 智也， 風間 裕介， 石井 公太郎， 大部 澄江， 白川 侑希， 阿部 知子
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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人為誘発非還元性雄性配偶子を活用した倍数性多様化法の開発
Grant number：20K06035 2020.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
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染色体微細加工で逆位・転座が植物ゲノムに与える影響を見る
Grant number：20H03297 2020.04 - 2023.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
Authorship：Coinvestigator(s)
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Establishment of analysis method for double fertilization process using unreduced male gamete
2017.04 - 2020.03
Grant-in-Aid for Scientific Research Grant-in-Aid for Young Scientists(B)
Authorship：Principal investigator
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高等植物における異型精細胞形成機構の解明
2010.04 - 2013.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
キルタンサスでは、花粉管伸長過程で一組の精細胞が異型化し、雄原細胞分裂直後の表層微小管構成時に蓄積の差異が生じる事が明らかとなった。精細胞形成の分子基盤情報を得るために遺伝子解析を行い、異型化特異的ステージの前後で発現量が変化する遺伝子の候補リストが得られた。花粉に重イオンビーム照射することで、雄性配偶子の研究に有用な新たな材料を作出するとともに、雄性配偶子のDNA損傷応答機構を明らかにした。
Other research activities 【 display / non-display 】
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Chrysanthemum x morifolium Ramat. ‘長崎SYC1’
2021.03
品種登録 出願番号35321
育成者：久村麻子、諸岡淳司、阿部知子、林依子、平野智也、石井公太郎 -
Chrysanthemum x morifolium Ramat. ‘長崎SWC1’
2019.03
品種登録 登録番号28537
育成者：久村麻子、諸岡淳司、阿部知子、林依子、平野智也 -
Prunus L. '仁科知花'
2016.06
品種登録 出願番号31199
育成者：石井重久、阿部知子、林 依子、平野智也 -
Chrysanthemum x morifolium Ramat. ‘白涼’
2015.03
品種登録 登録番号26306
育成者：峯大樹、岳田司、久村麻子、阿部知子、平野智也、林依子 -
Prunus L. ‘仁科小町’
2012.09
品種登録 出願番号27386
育成者：石井重久、阿部知子、林依子、福西暢尚、平野智也
Available Technology 【 display / non-display 】
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微細操作技術を活用した植物の受精機構に関する研究
突然変異育種法の効率化に関する研究
ラン科植物の長期遺伝資源保存法の開発Home Page： 植物遺伝育種学研究室
Related fields where technical consultation is available：・花き植物を中心とした突然変異育種
・植物遺伝資源の保存、植物組織培養による増殖
・突然変異遺伝子の同定、単離Message：・共同研究の希望テーマ：花き園芸植物の品種改良
・少数細胞解析技術の開発ニーズがあれば、ぜひご連絡ください。