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Affiliation |
Faculty of Agriculture Department of Agricultural and Environmental Sciences |
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Professor |
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TAKESHITA Minoru
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Research Areas 【 display / non-display 】
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Environmental Science/Agriculture Science / Plant protection science
Papers 【 display / non-display 】
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Ota E., Masuta C., Takeshita M.
Plant Pathology Journal 39 ( 6 ) 592 - 599 2023.12
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Plant Pathology Journal
A defective RNA3 (D3Yα) of strain Y of cucumber mosaic virus (CMV-Y) was examined on host-specific maintenance, experimental conditions, and a viral factor required for its generation in plants. D3Yα was stably maintained in cucumber but not in tomato plants for 28 days post inoculation (dpi). D3Yα was generated in Nicotiana tabacum or N. benthamiana after prolonged infection in the second and the third passages, but not in plants of N. benthamiana grown at low temperature at 28 dpi or infected with CMV-Y mutant that had the 2b gene deleted. Collectively, we suggest that generation and retention of D3Yα depends on potential host plants and experimental conditions, and that the 2b protein has a role for facilitation of generation of D3Yα.
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Host-specific adaptation drove the coevolution of leek yellow stripe virus and Allium plants Reviewed
Kawakubo S., Kim H., Takeshita M., Masuta C.
Microbiology Spectrum 11 ( 5 ) 2023.10
Language：English Publishing type：Research paper (scientific journal) Publisher：Microbiology Spectrum
Host adaptation plays a crucial role in virus evolution and is a consequence of long-term interactions between virus and host in a complex arms race between host RNA silencing and viral RNA silencing suppressor (RSS) as counterdefense. Leek yellow stripe virus (LYSV), a potyvirus causing yield loss of garlic, infects several species of Allium plants. The unexpected discovery of an interspecific hybrid of garlic, leek, and great-headed (GH) garlic motivated us to explore the host-adaptive evolution of LYSV. Here, using Bayesian phylogenetic comparative methods and a functional assay of viral RSS activity, we show that the evolutionary context of LYSV has been shaped by the host adaptation of the virus during its coevolution with Allium plants. Our phylogenetic analysis revealed that LYSV isolates from leek and their taxonomic relatives (Allium ampeloprasum complex; AAC) formed a distinct monophyletic clade separate from garlic isolates and are likely to be uniquely adapted to AAC. Our comparative studies on viral accumulation indicated that LYSV accumulated at a low level in leek, whereas LYSVs were abundant in other Allium species such as garlic and its relatives. When RSS activity of the viral P1 and HC-Pro of leek LYSV isolate was analyzed, significant synergism in RSS activity between the two proteins was observed in leek but not in other species, suggesting that viral RSS activity may be important for the viral host-specific adaptation. We thus consider that LYSV may have undergone host-specific evolution at least in leek, which must be driven by speciation of its Allium hosts.
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Kobayashi Y., Hyodo A., Kuwata S., Masuta C., Takeshita M.
Journal of General Plant Pathology 89 ( 2 ) 109 - 121 2023.3
Authorship：Corresponding author Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Journal of General Plant Pathology
Chemically induced host resistance is useful phenomenon for comprehending host innate immunity against plant pathogens. To evaluate immune priming against cucumber mosaic virus (CMV) infection, we sprayed benzothiadiazole (BTH), a salicylic acid (SA) analog, on either one-leaf or entire plants of Nicotiana benthamiana (Nb). The chemical immune priming downregulated transcript level of Salicylic acid glucosyltransferase (SAGT) and upregulated that of Pathogenesis-related protein 1a (PR1a) in the treated and untreated upper leaves of the one-leaf-treated Nb. Fluorescent-protein-expressing CMV vectors revealed inhibitory effect on initial infection and delayed systemic infection in the one-leaf-treated Nb inoculated with the virus vectors, and the BTH was more effective than SA. However, the BTH-induced host defensive response was attenuated when wild-type Nb was infected with the virus before BTH spray of one leaf and by constitutive expression of the 2b protein of CMV (2b) in a transgenic Nb line after BTH spray of one leaf. SAGT was downregulated and PR1a upregulated in the upper, middle, and lower leaves when the entire plant was treated with BTH. Viral levels were reduced in BTH-treated middle and -untreated upper leaves, but not in the emerging and uppermost leaves after the whole plant spray. Taken together, these results suggest that the chemical immune priming is more effective than SA to retard systemic infection of CMV. However, the BTH-mediated antiviral responses can be attenuated by prior CMV infection and by constitutive expression of 2b in Nb, and does not efficiently suppress CMV infection in later-emerging leaves.
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A next-generation-sequencing approach to diagnose viral diseases of greenhouse ranunculus Reviewed
Hayahi S., Ota E., Kurogi S., Matsushita Y., Kanno Y., Takeshita M.
Journal of General Plant Pathology 88 ( 2 ) 134 - 137 2022.3
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of General Plant Pathology
Viruses that infect Ranunculus asiaticus L., a globally important greenhouse ornamental, have consistently caused serious damage in Japan. We coupled nextgeneration-sequencing (NGS) with visual assessment of symptoms on leaf tissues, and found almost all of the diseases were caused by ranunculus mild mosaic virus (RanMMV) and ranunculus leaf distortion virus (RanLDV). These results were confirmed by reverse transcription-polymerase chain reaction specific to viral genes of ranunculus-infecting viruses. NGS with symptom assessment should be able to provide a rapid and practical diagnostic method focusing on RanMMV and RanLDV in Japan.
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Plant viruses and viroids in Japan Reviewed
Fuji S.i., Mochizuki T., Okuda M., Tsuda S., Kagiwada S., Sekine K.T., Ugaki M., Natsuaki K.T., Isogai M., Maoka T., Takeshita M., Yoshikawa N., Mise K., Sasaya T., Kondo H., Kubota K., Yamaji Y., Iwanami T., Ohshima K., Kobayashi K., Hataya T., Sano T., Suzuki N.
Journal of General Plant Pathology 88 ( 2 ) 105 - 127 2022.3
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of General Plant Pathology
An increasing number of plant viruses and viroids have been reported from all over the world due largely to metavirogenomics approaches with technological innovation. Herein, the official changes of virus taxonomy, including the establishment of megataxonomy and amendments of the codes of virus classification and nomenclature, recently made by the International Committee on Taxonomy of Viruses were summarized. The continued efforts of the plant virology community of Japan to index all plant viruses and viroids occurring in Japan, which represent 407 viruses, including 303 virus species and 104 unclassified viruses, and 25 viroids, including 20 species and 5 unclassified viroids, as of October 2021, were also introduced. These viruses and viroids are collectively classified into 81 genera within 26 families of 3 kingdoms (Shotokuvirae, Orthornavirae, Pararnavirae) across 2 realms (Monodnaviria and Riboviria). This review also overviewed how Japan’s plant virus/viroid studies have contributed to advance virus/viroid taxonomy.
Books 【 display / non-display 】
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紫外線（UV）照射によるイチゴうどんこ病に 対する抵抗性誘導の評価法開発
太田江美、 竹下 稔、西村文宏、森 充隆、神頭武嗣、田中雅也、細川宗孝、刑部正博、佐藤 衛（ Role： Joint author）
日本植物防疫協会 2021.12
Language：Japanese Book type：Scholarly book
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サツマイモ病害診断・観察簡易マニュアル
小倉李来, 竹下 稔（ Role： Joint author）
国立大学法人宮崎大学農学部植物病理学研究室 2021.2
Language：Japanese Book type：Report
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（株）共立電機製作所・（株）共立電照との共同研究報告書
太田江美、松浦昌平、竹下 稔（ Role： Joint author）
宮崎大学 2020.4
Language：Japanese Book type：Report
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Cucumber mosaic virus
Minoru Takeshita（ Role： Joint author）
APS Press 2018.12
Language：English Book type：Scholarly book
MISC 【 display / non-display 】
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植物成長と防御応答の調節へのサリチル酸配糖化酵素遺伝子の関与 Invited
竹下 稔、小林優大
植物感染生理談話会論文集 ( 56 ) 77 - 86 2022.9
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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トマトモザイクウイルス感染によるホオズキの生育への影響と乾熱処理種子由来の地下茎導入によるウイルス病害抑制効果 Invited
早日早貴、黒木修一、竹下 稔
植物防疫 76 ( 7 ) 13 - 16 2022.7
Authorship：Last author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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紫外光照射によるイチゴうどんこ病抑制効果と抵抗性遺伝子の解析 Reviewed
太田江美、西村文宏、佐藤 衛、竹下 稔
日本植物病理学会報 2021.1
Language：Japanese Publishing type：Research paper, summary (national, other academic conference)
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サツマイモのウイルス病害診断技術の改良と栽培ほ場での疫学的調査 Reviewed
小倉李来、奈良迫洋介、山崎修一、竹下 稔
日本植物病理学会報 2021.1
Language：Japanese Publishing type：Research paper, summary (national, other academic conference)
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ユズ圃場由来のカンキツトリステザウイルスの遺伝子型解析 Reviewed
高田佑哉、山名宏美、金丸俊徳、山口秀一、竹下 稔
日本植物病理学会報 2020.8
Language：Japanese Publishing type：Research paper, summary (national, other academic conference)
Presentations 【 display / non-display 】
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Polyphenol markers for selection of sweet potato cultivars resistant to sweet potato foot rot.
2024.3.28
Event date： 2024.3.28 - 2024.3.29
Presentation type：Oral presentation (general)
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キュウリモザイクウイルス媒介試験におけるUV-B照射がおよぼすモモアカアブラムシ定住パターンへの影響 International coauthorship
太田江美, 松浦昌平, Alex Murphy, John Carr, 竹下 稔
令和6年度日本植物病理学会大会 2024.3.13
Event date： 2024.3.13 - 2024.3.15
Language：Japanese Presentation type：Oral presentation (general)
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UV-B treatment suppresses symptoms induced by cucumber mosaic virus but does not affect vectors’ preferences on tomato plants
Emi Ota, Shohei Matsuura, Minoru Takeshita
令和５年度日本植物病理学会九州部会 2023.11.28
Event date： 2023.11.28 - 2023.11.29
Language：English Presentation type：Oral presentation (general)
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First report of sweet potato virus 2 infecting sweet potato in Japan
2023.11.28
Event date： 2023.11.28 - 2023.11.29
Language：Japanese Presentation type：Oral presentation (general)
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Host-adaptive evolution of leek yellow stripe virus determined by viral RNA silencing suppressor activity in a host-dependent manner
Shusuke Kawakubo, Hangil Kim, Minoru Takeshita, Chikara Masuta
The 70th Annual Meeting of the Japanese Society for Virology
Event date： 2023.9.26 - 2023.9.28
Language：English Presentation type：Oral presentation (general)
Awards 【 display / non-display 】
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令和4年度公募型卒業研究テーマ成果発表会ベストポスター賞
2023.3 宮崎県高等教育コンソーシアム 宮崎市内のビロウ並木の天狗巣・黄化・枯死症状の原因究明と対策
青山実樹、安達鉄矢、山本昭洋、佐伯雄一、櫛間義幸、土屋健一、竹下 稔
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平成17年度日本植物病理学会学術奨励賞
2005.3 日本植物病理学会
竹下 稔
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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植物の病害抵抗性反応とバイオマス生産の制御に関与する分子機構の解明
Grant number：21J15403 2021.04 - 2023.03
独立行政法人日本学術振興会 科学研究費補助金 特別研究員奨励費
Authorship：Principal investigator
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サリチル酸配糖化酵素を介した植物ウイルス間の病原性相乗作用に関する分子機構の解明
2020.04 - 2021.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
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サリチル酸配糖化酵素を介した植物ウイルス間の病原性相乗作用に関する分子機構の解明
2019.04 - 2020.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
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サリチル酸配糖化酵素を介した植物ウイルス間の病原性相乗作用に関する分子機構の解明
2018.04 - 2019.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
Other research activities 【 display / non-display 】
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Effects of salicylic acid treatment on accumulation level of cucumber mosaic virus and aphids’ settlement on pepper plants
2023.04 - 2024.03