津山 濯 (ツヤマ タク)

TSUYAMA Taku

写真a

職名

助教

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  • 京都大学 -  博士(農学)

専門分野(科研費分類) 【 表示 / 非表示

  • 木質科学

 

研究経歴 【 表示 / 非表示

  • リグニン前駆物質の輸送メカニズム

    (選択しない)  

    研究期間: 2007年04月  -  継続中

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  • Down-regulation of pyruvate decarboxylase gene of white-rot fungus Phlebia sp. MG-60 modify the metabolism of sugars and productivity of extracellular peroxidase activity.

    Motoda T, Yamaguchi M, Tsuyama T, Kamei I

    Journal of bioscience and bioengineering     2018年07月

    共著

    DOI PubMed

  • Lignification in developing culms of bamboo Sinobambusa tootsik

    Tsuyama T., Shimada N., Motoda T., Matsushita Y., Kijidani Y., Fukushima K., Kamei I.

    Journal of Wood Science   63 ( 6 ) 551 - 559   2017年12月  [査読有り]

    共著

     概要を見る

    © 2017, The Japan Wood Research Society. Bamboos are among the largest woody grasses and grow very rapidly. Although lignin is a crucial factor for the utilization of bamboo biomass, the lignification mechanism of bamboo shoots is poorly understood. We studied lignification in the bamboo Sinobambusa tootsik during culm development. Elongation growth began in May and ended in late-June, when the lignin content was approximately half that in mature culms. Thioacidolysis analysis indicated that p-hydroxyphenyl units in lignin formed even at late stages of lignification. The syringyl/guaiacyl ratio varied during culm development. Various lignin precursors were detected in developing culms by liquid chromatography–mass spectrometry. The ferulic acid content decreased from May to June, indicating that ferulic acid was utilized in early stages of cell wall formation. Monolignol glucosides were detected at early stages of lignification, whereas the contents of monolignols, coniferaldehyde, sinapaldehyde, p-coumaric acid, and ferulic acid peaked at later stages of lignification. Therefore, lignin precursors may be supplied differentially during the lignification process. In August, the rate of lignification decreased, although the contents of various lignin precursors peaked, implying that the rate-limiting step in the cessation of lignification in bamboo is transport or polymerization of lignin precursors, rather than their biosynthesis.

    DOI

  • Accumulation of sugar from pulp and xylitol from xylose by pyruvate decarboxylase-negative white-rot fungus Phlebia sp. MG-60

    Tsuyama T., Yamaguchi M., Kamei I.

    Bioresource Technology   238   241 - 247   2017年08月  [査読有り]

    共著

     概要を見る

    © 2017Phlebia sp. MG-60 is a white-rot fungus that produces ethanol with high efficiency from lignocellulosic biomass without additional enzymes. Through engineering of this powerful metabolic pathway for fermentation in Phlebia sp. MG-60, chemical compounds other than ethanol could be produced. Here, we demonstrate sugar accumulation from unbleached hardwood kraft pulp and conversion of xylose to xylitol by pyruvate decarboxylase (pdc)-negative Phlebia sp. MG-60. We isolated Phlebia sp. strain MG-60-P2 from protoplasts to unify the protoplast phenotypes of the regenerated strains. Homologous recombination achieved a stable pdc-knockout line, designated KO77. The KO77 line produced traces of ethanol, but accumulated xylitol from xylose or glucose from unbleached hardwood kraft pulp. These metabolic changes in the pdc-knockout strain reflect the potential of metabolic engineering in Phlebia sp. MG-60 for direct production of chemical compounds from lignocellulosic biomass.

    DOI

  • Seasonal variations of tracheid formation and amount of auxin (IAA) and gibberellin A4 (GA4) in cambial-region tissues of mature sugi (Cryptomeria japonica) cultivar grown in a Nelder plot with different tree densities

    Kijidani Y., Nagai T., Suwashita T., Tsuyama T.

    Journal of Wood Science   63 ( 4 ) 315 - 321   2017年08月  [査読有り]

    共著

     概要を見る

    © 2017, The Japan Wood Research Society. The role of phytohormones in tracheid formation remains unclear in conifers. In this study, to obtain information on the role of auxin (IAA) and gibberellin A4 (GA4), we examined seasonal variation in the amount of phytohormones in cambial-region tissues and tracheid formation of sugi cultivar planted in a Nelder plot with different tree densities. We demonstrated that the amount of IAA was positively correlated with the number of tracheids formed in early and mid-season, but not in late season, and had no relation to tracheid differentiation. Crown length and height at the crown base had a positive and negative effect, respectively, on IAA amounts in early and mid-season, but not in late season. Height-to-diameter ratio was negatively correlated with IAA amounts in early and mid-season, but not in late season. Sugi trees with wider spacing continued tracheid formation in late season with smaller amounts of IAA, although the trees with narrower spacing ceased tracheid formation with larger amounts of IAA. Cambial growth cessation in late season might be controlled not by IAA amount, but by short-day-induced insensitivity to IAA. GA4 had no relation with the indexes of growth traits or tracheid formation.

    DOI

  • Coniferin β-glucosidase is ionically bound to cell wall in differentiating xylem of poplar

    Tsuyama T., Takabe K.

    Journal of Wood Science   61 ( 4 ) 438 - 444   2015年08月  [査読有り]

    共著

     概要を見る

    © 2015, The Japan Wood Research Society. Lignin is crucial for the growth and persistence of vascular plants in diverse environments. Although biosynthesis and polymerization of lignin precursors have been subjected to in-depth studies, only limited information exists on the ways in which these precursors are transported. Recent research has demonstrated that V-ATPase-dependent transport of coniferyl alcohol glucoside (coniferin) is a major metabolic function in differentiating xylem of both angiosperms and gymnosperms. Localization of β-glucosidase involved in the cleavage of coniferin to coniferyl alcohol and glucose is important to clarify the role of coniferin in lignification; nevertheless, only little has been reported in angiosperms. Here, we demonstrated the presence of coniferin β-glucosidase activity in a cell wall ionically bound protein fraction extracted from differentiating xylem of poplar. Coniferin β-glucosidase localization is very likely in cell wall similar to previous reports in conifers. We identified a putative poplar coniferin β-glucosidase through phylogenetic analysis and named this protein “PtrBGL6”. Immunoprecipitation assays showed that the anti-PtrBGL6 antibody recognizes a coniferin β-glucosidase in a cell wall ionically bound protein fraction. Conserved coniferin β-glucosidases localized in cell wall in both angiosperm and gymnosperm implies their important roles in the formation of lignified cell wall.

    DOI

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学術関係受賞 【 表示 / 非表示

  • 第28回 日本木材学会奨励賞

    2017年03月   日本木材学会  

    受賞者:  津山 濯

  • Best Poster Presentation

    2009年08月   Pacific Regional Wood Anatomy Conference  

    受賞者:  Taku Tsuyama, Keiji Takabe

科研費(文科省・学振)獲得実績 【 表示 / 非表示

  • ポプラ分化中木部におけるコニフェリン輸送体の同定

    若手研究(B)

    研究期間:  2017年04月  -  2020年03月  代表者:  津山 濯

  • 分化中木部におけるリグニン前駆物質輸送メカニズムの解明

    特別研究員奨励費

    研究期間:  2011年04月  -  2013年03月 

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  • Shikonin production of Lithospermum erythrorhizon, a model system of secondary metabolic lipids in plants

    Kazufumi Yazaki, Kanade Tatsumi, Takuji Ichino, Hideaki Matsuoka, Fumihiko Sato, Mayuko Sato, Taku Tsuyama, Kiminori Toyooka

    23rd International Symposium on Plant Lipid  2018年07月  -  2018年07月   

  • 樹幹傾斜による引張あて材形成と内生植物ホルモンの偏差分布について

    冨家 梓、津山 濯、雉子谷佳男

    第68回日本木材学会大会  2018年03月  -  2018年03月   

  • ポプラ分化中木部におけるp-グルコクマリルアルコールの輸送

    津山 濯、松下泰幸、福島和彦、矢崎一史、高部圭司、亀井一郎

    第68回日本木材学会大会  2018年03月  -  2018年03月   

  • タケにおけるチオグリコール酸リグニン法の迅速化

    島田菜津美、津山 濯、亀井一郎

    第68回日本木材学会大会  2018年03月  -  2018年03月   

  • 白色腐朽菌カワラタケ(Trametes versicolor)が生成する細胞外ナノベシクルの探索

    小川美香,津山 濯,亀井一郎

    第68回日本木材学会大会  2018年03月  -  2018年03月   

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