Date of Birth


Laboratory Address

gakuennkibanadai1-1, Miyazaki, 889-2192, JAPAN

Research Fields, Keywords

Life science

Mail Address

E-mail address

Laboratory Phone number


Laboratory Fax number


Graduating School 【 display / non-display


    Saga University   Faculty of Agriculture   Department of Horticulture   Graduated

Graduate School 【 display / non-display


    Chiba University  Graduate School, Division of Agricultural Science  Doctor's Course  Completed

Degree 【 display / non-display

  • Chiba University -  Doctor (Ph.D.)


Papers 【 display / non-display

  • Morphological Characterization and Evaluation of Reproductive Function in a Haploid Pummelo [<i>Citrus maxima</i> (Burm.) Merr.]

    YAHATA Masaki, KUNITAKE Hisato, KOMATSU Haruki

    Japan Agricultural Research Quarterly: JARQ   51   293 - 298   2017.10  [Refereed]

    Joint Work

     View Summary

    <p>To obtain basic information about haploid plants in <i>Citrus</i>, the morphological characteristics and reproductive potential of a haploid plant obtained from the 'Banpeiyu' pummelo [<i>C. maxima</i> (Burm.) Merr.] were examined. The leaves, flowers and fruits of the haploid were significantly smaller than those of the 'Banpeiyu' pummelo. Although the haploid showed remarkably reduced pollen fertility compared with the 'Banpeiyu' pummelo, it produced slightly fertile pollen grains. In crosses with diploid cultivars, when the haploid was the seed parent, there was no seed. But when diploid cultivars were pollinated with pollen of the haploid, diploid progenies were obtained. During female gamete formation, no embryo-sac mother cell was formed in the ovules, and consequently no normal embryo sac was observed at anthesis, owing to female sterility caused by the lack of embryo-sac mother cell (EMC) formation in the haploid. During male gamete formation, however, univalents were not distributed to opposite poles at anaphase I in some dividing cells; instead, they remained near the equatorial plate. The nine univalents then performed mitosis, and the univalents were equally distributed to opposite poles. And at the tetrad stage, dyads were also produced in the haploid at a ratio of 24.7%. These results suggest that fertile pollen grains in the haploid would be dyad derivation, produced by abnormalities in the first meiotic division such as first division restitution (FDR).</p>

    DOI CiNii

  • Growth Characteristics and Fruit Qualities of Interspecific Hybrid between `Indian Summer' Raspberry and Rubus parvifolius L.

    Horticultural research   16   345 - 352   2017.08  [Refereed]

    Single Work

     View Summary

    In order to develop new raspberry varieties adaptable to Japan with high quality and functionality, we aimed to produce interspecific hybrids between Rubus parvifolius L., a wild species native to Japan, and the `Indian Summer' raspberry (R. idaeus L.). The morphological characteristics, such as the leaf, flower, and fruit, and random amplified polymorphic DNA (RAPD) analysis revealed that `07RUBIXP01' was an interspecific hybrid. Although `07RUBIXP01' had small thorns, it showed vigorous growth in the warm environment and had a significantly larger fruit compared with the parents. Also, `07RUBIXP01' showed values intermediate between those of the parents for total polyphenol, anthocyanin, and ellagitannin contents. In particular, it showed an ellagitannin content that was about four times higher than that of `Indian Summer'. In sugar and organic acid analyses, a higher sugar acid ratio was noted compared with the parents. `07RUBIXP01' was registered as a new variety in 2012 (variety registration no. 21801), and it would be a useful raspberry for growing in warm regions of Japan.


  • Cryopreservation of blueberry shoot tips derived from in vitro and current shoots using D cryo-plate technique

    Dhungana Sabitri Adhikari, Kunitake Hisato, Niino Takao, Yamamoto Shin-ichi, Fukui Kuniaki, Tanaka Daisuke, Maki Shinya, Matsumoto Toshikazu

    Plant Biotechnology   34   1 - 5   2017.03  [Refereed]

    Joint Work

     View Summary

    Cryopreservation is an important tool for long-term storage of plant germplasm that is currently used for plant germplasm storage at many institutes worldwide. Recently, novel cryogenic procedures (V and D cryo-plate methods) have been developed. In this study, the most suitable conditions for preserving blueberry shoot tips derived from in vitro and current shoots using the D cryo-plate method were investigated. The D cryo-plate method has advantages such as higher regrowth after cryopreservation and a more user-friendly process compared with conventional cryogenic methods. The optimum duration of desiccation for regrowth of shoot tips from each shoot type was 1 h. To induce dehydration tolerance for the shoot tips, the effects of two cryoprotection treatments (sucrose preculture and loading solution [LS] treatment) on shoot regrowth after cryopreservation were investigated. The combined effect of both treatments significantly increased percentage regrowth (approximately 90%). No regrowth of shoot tips was attained without the two treatments. Thus, preculture and LS treatment were effective to induce dehydration tolerance for cryopreservation of blueberry shoot tips. The optimized conditions for blueberry shoot tips using the D cryo-plate technique were: preculture with 0.3 M sucrose for 1 day, LS treatment (2 M glycerol +0.4–1.0 M sucrose) for 30 min, and air dehydration for 1 h. This optimized procedure was applied to additional blueberry cultivars shoot tips derived from in vitro shoots (regrowth 46.7–100%) and current shoots (regrowth 17.2–62.7%). Furthermore, in vitro shoot tips were suitable material for the D cryo-plate method in blueberry.

    DOI CiNii

  • Phylogeny and Classification of Kumquats (Fortunella spp.) Inferred from CMA Karyotype Composition

    Kiichi Yasuda, Masaki Yahata and Hisato Kunitake

    The Horticulture Journal   85   115 - 121   2016.03  [Refereed]

    Joint Work


  • In Vitro Self-incompatible-like Response Applied for Protein Identification and Gene Expression Analysis in Citrus Cultivars, Banpeiyu and Hyuganatsu

    Li, Y., Abe, A., Fuse, T., Hirano, T., Hoshino, Y., Kunitake, H.

    Journal of the American Society for Horticultural Science   140   339 - 345   2015.08

    Joint Work

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Books 【 display / non-display

  • 植物育種学辞典(共著)

    監修者:鵜飼保雄、大澤良、堤伸浩、長峰司、二宮正司、平田豊、藤垣順三、三位正洋共著者:國武久登ほか、日本育種学会関係者350名 (Part: Joint Work )

    培風館(東京)全785頁、分担 10語  2005.08 ISBN: 4-563-07788-7

  • Biotechnology in Agriculture and Forestry, Vol. 49 Somatic hybridization in crop improvement Ⅱ

    編集者:Bajaj, Y.P.S. 共著者:Kunitake, H., M. Mii (Part: Joint Work )

    Springer-Verlag (Germany)全377頁分担 95-111頁  2001.07

  • Biotechnology in Agrculture and Forestry, Vol. 34 Plant protoplasts and genetic engineering Ⅵ

    編集者:Bajaj, Y.P.S. 共著者:Takahata, Y., H. Jyomori, S. Miyano, H. Kunitake, M. Mii (Part: Joint Work )

    Springer-Verlag (Germany)全451頁分担 55-62頁  1995.12

  • Biotechnology in Agriculture and Forestry, Vol. 30 Somatic embryogenesis and synthetic seed Ⅰ

    編集者:Bajaj, Y.P.S. 共著者:Kunitake, H., M. Mii (Part: Joint Work )

    Springer-Verlag (Germany)全472頁分担 280-298頁  1995.02

  • Biotechnology in Agriculture and Forestry, Vol. 29 Plant protoplasts and geneticengineering.Ⅴ

    編集者:Bajaj, Y.P.S.共著者:Kunitake, H., M. Mii (Part: Joint Work )

    Springer-Verlag (Germany)全414頁分担 72-82頁  1994.12

Review Papers 【 display / non-display

  • The phenomenon of root elongation and high respiration actibity in the rolB-gene-enhanced FSL#35 variant of Lotus corniculatus FOX-SR line.

    Yano Tsubasa, Yamamoto Akihiro, Kunitake Hisato, Saeki Yuichi, Akashi Ryo

    Plant Root   8 ( 0 ) 82 - 91   2014.08

    Introduction and explanation (bulletin of university, research institution)   Joint Work

    DOI CiNii

  • Comparison of cultivars and seasonal variation in blueberry (Vaccinium species) leaf extract on Adult T-cell leukemia cell line growth suppression.

    Kai Hisahiro, Fuse Takuichi, Kunitake Hisato, Morishita Kazuhiro and Matsuno Koji

    Medicines   1 ( 1 ) 3 - 11   2014.06

    Introduction and explanation (bulletin of university, research institution)   Joint Work


  • Gardening plants, Citrus species.

    Hisato Kunitake

      57 ( 4 ) 4 - 7   2007.04

    Introduction and explanation (commerce magazine)   Single Work

Presentations 【 display / non-display

  • SSRマーカーによる半数体ブンタンの遺伝子型解析


    園芸学会平成29年度秋季大会  2017.09  -  2017.09  園芸学会

     View Summary


  • みやだいCOC事業の事例紹介

    國武 久登

    平成28年度COC/COC+全国シンポジウム  2017.03  -  2017.03  高知大学

  • Effect of temperature on self-incompatibility in Citrus pistil and mature pollen culture systems.

    Kawano S., Kunitake H., Li. Y., Yahata M.

    The Third International Symposuim on Citrus Biotechnology  2014.11  -  2014.11  ISHS

  • Self-incompatibility-like response induces alteration of actin cytoskeleton and cytosolic calsium in the Citrus pollen tube of Banpeiyu and Hyuganatsu.

    Li Y., Fuse T., Kawano S. Kunitake H

    The Third International Symposium on Citrus Biotechnology   2014.11  -  2014.11  ISHS

  • Protein identification and gene expression analysis of in vitro pollen tubes in self-incompatibility-like response of citrus cultivars Banpeiytu and Hyuganatsu.

    Li. Y., Fuse T., Abe A., Uchida A., Hoshino Y., Kunitake H.

    園芸学会平成26年度秋季大会  2014.09  -  2014.09  園芸学会

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Preferred joint research theme 【 display / non-display

  • Production of new blueberry cultivars

  • Production of new kumquat cultivars

  • Production of new raspberry cultivars

  • Biotechnology of fruit crops