Date of Birth


Laboratory Address

gakuennkibanadai1-1, Miyazaki, 889-2192, JAPAN

Research Fields, Keywords

Life science

Mail Address

E-mail address

Laboratory Phone number


Laboratory Fax number


Graduating School 【 display / non-display


    Saga University   Faculty of Agriculture   Department of Horticulture   Graduated

Graduate School 【 display / non-display


    Chiba University  Graduate School, Division of Agricultural Science  Doctor's Course  Completed

Degree 【 display / non-display

  • Chiba University -  Doctor (Ph.D.)


Papers 【 display / non-display

  • Blueberry Leaf Polyphenols Prevent Body Fat Accumulation in Mice Fed High-fat, High-sucrose Diet.

    Fujii K, Ota Y, Nishiyama K, Kunitake H, Yamasaki Y, Tari H, Araki K, Arakawa T, Yamasaki M

    Journal of oleo science     2019.04  [Refereed]

    Joint Work

    DOI PubMed

  • Subchronic toxicity evaluation of leaves from rabbiteye blueberry (Vaccinium virgatum Aiton) in rats

    Tanaka , Daigo Yokoyama ⁠, Yasushi Matsuura , Masahiko Nozaki ⁠, Naoya Hirozawa ⁠,Naoya Hirozawa ,Hisato Kunitake ⁠, Masanobu Sakono ⁠, Hiroyuki Sakakibara

    Toxicology Reports   6   272 - 278   2019.03  [Refereed]

    Joint Work

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    Blueberry leaf may contain multiple compounds with beneficial effects. We conducted a 90-day toxicity study in rats to evaluate the safety of consuming the leaves of rabbiteye blueberry (Vaccinium virgatum Aiton; RB species). Powdered leaves were administered daily by oral gavage at doses of 500, 1000, and 2500 mg/kg body weight to male and female Sprague-Dawley rats for 90 days. Treatment did not result in death or changes in the behavior and external appearance of the animals. No alterations were observed in hematological and serum chemical parameters, urinalysis, food consumption, body weight gain, or absolute and relative organ weights at the end of the treatment period, with the exception of some leukocyte percentages in male rats treated with 500 and 1000 mg/kg blueberry leaf powder. The findings indicate that rabbiteye blueberry leaf is safe for consumption and should be investigated as a candidate functional food.

  • Characteristics in autotetraploid kumquats (Fortunella spp.) induced by colchicine treatment to nucellar embryos and their utilization for triploid breeding

    Nukaya T., Sudo M., Yahata M., Nakajo Y., Ohta T., Yasuda K., Tominaga A., Mukai H., Kunitake H.

    Scientia Horticulturae   245   210 - 217   2019.02  [Refereed]

    Joint Work

     View Summary

    © 2018 Elsevier B.V. Three Fortunella (kumquat) species, the Meiwa kumquat (F. crassifolia Swingle), the Round kumquat [F. japonica (Thunb.) Swingle] and the Changshou kumquat (F. obovata hort. ex Tanaka), showing tetraploid, which had been induced by treating the seeds with colchicine, were examined for origin and horticultural characteristics (e. g. morphology of leaves, flowers, pollen and fruits). Additionally, these tetraploid kumquats were crossed to produce triploid kumquats. All of the tetraploids were confirmed to be derived from the nucellar embryo of each original kumquat by examining their chromosomal composition with chromomycin A3 staining. All of the tetraploid kumquats had the typical morphological characteristics of tetraploid Citrus plants, such as round and thick leaves, and large flowers and pollen grains. On the other hand, the fruits of these tetraploid plants showed desirable traits for kumquats such as thicker pericarp and higher soluble solids content. Furthermore, when these tetraploids were crossed with some diploid cultivars, triploid progenies were obtained from almost all of the cross combinations.


  • Effects of blueberry leaf and stem extracts on hepatic lipid levels in rats consuming a high-sucrose diet

    Yasushi Matsuura, Hiroyuki Sakakibara, Maho Kawaguchi, Emi Murayama, Daigo Yokoyama, Chizuko Yukizaki, Hisato Kunitake, Masanobu Sakono

    Functional Foods in Health and Disease   8   447 - 461   2018.09  [Refereed]

    Joint Work

     View Summary

    Background: Blueberry stems, a by-product of blueberry leaf tea production, are typically discarded. We evaluated the effects of hot-water extracts of rabbiteye blueberry (Vaccinium virgatum Aiton; RB species) leaves and stems on hepatic lipid levels in rats consuming a high-sucrose diet. Methods: Male Sprague-Dawley rats were divided into groups that received a control high-sucrose diet alone or supplementation with 2.0% blueberry leaf extract or 0.5% or 2.0% blueberry stem extract. Blood and hepatic lipid levels, hepatic lipogenic enzyme activity, and hepatic quercetin metabolites were evaluated after 28 days of ad libitum consumption. Results: Supplementation with the extracts did not affect body weight gain, food intake, liver and white adipose tissue weights, or serum lipid levels. Hepatic triglyceride and total cholesterol levels were reduced in the groups that received 2.0% supplementation of either extract. Hepatic malic enzyme activity was also reduced in those groups. Quercetin and its glycosides, the major polyphenols identified in the extracts, accumulated in the liver as quercetin aglycone and quercetin metabolites. Conclusion: We demonstrated how daily consumption of blueberry leaf and stem extracts can decrease hepatic lipid levels, potentially downregulating malic enzyme activity. These effects were intensive in leaf extracts. The active compounds existed in both extracts may be quercetin and its glycosides. Therefore, blueberry stems and leaves may be an attractive candidate novel functional food.


  • Morphological Characterization and Evaluation of Reproductive Function in a Haploid Pummelo [<i>Citrus maxima</i> (Burm.) Merr.]

    YAHATA Masaki, KUNITAKE Hisato, KOMATSU Haruki

    Japan Agricultural Research Quarterly: JARQ   51   293 - 298   2017.10  [Refereed]

    Joint Work

     View Summary

    <p>To obtain basic information about haploid plants in <i>Citrus</i>, the morphological characteristics and reproductive potential of a haploid plant obtained from the 'Banpeiyu' pummelo [<i>C. maxima</i> (Burm.) Merr.] were examined. The leaves, flowers and fruits of the haploid were significantly smaller than those of the 'Banpeiyu' pummelo. Although the haploid showed remarkably reduced pollen fertility compared with the 'Banpeiyu' pummelo, it produced slightly fertile pollen grains. In crosses with diploid cultivars, when the haploid was the seed parent, there was no seed. But when diploid cultivars were pollinated with pollen of the haploid, diploid progenies were obtained. During female gamete formation, no embryo-sac mother cell was formed in the ovules, and consequently no normal embryo sac was observed at anthesis, owing to female sterility caused by the lack of embryo-sac mother cell (EMC) formation in the haploid. During male gamete formation, however, univalents were not distributed to opposite poles at anaphase I in some dividing cells; instead, they remained near the equatorial plate. The nine univalents then performed mitosis, and the univalents were equally distributed to opposite poles. And at the tetrad stage, dyads were also produced in the haploid at a ratio of 24.7%. These results suggest that fertile pollen grains in the haploid would be dyad derivation, produced by abnormalities in the first meiotic division such as first division restitution (FDR).</p>

    DOI CiNii

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Books 【 display / non-display

  • Flowering and Fruiting Haploid and Doubled Haploid Pummelos

    Masaki Yahata、Hisato Kunitake (Part: Joint Work ,  執筆 )

    IntechOpen  2019.02 ISBN: 978-1-78985-428-2

     View Summary

    Haploid and doubled haploid (DH) plants are of great value for genetic analyses and premeditated breeding. This is especially true for woody species, which are generally characterized by a long reproductive cycle, a high degree of heterozygosity, a large plant size, and self-incompatibility. In Citrus and related genera, some haploid and DH plants have been produced by techniques such as anther culture, interploid hybridization, and the pollination of irradiated pollen. However, there are few reports of the characteristics of haploid and DH plants’ flowers, fruits, or reproductive potential. We selected a haploid progeny among small seed-derived seedlings obtained from ‘Banpeiyu’ pummelo [C. maxima (Burm.) Merr.], and we produced the DH plant of this haploid using colchicine-treated axillary shoot buds. Both this haploid pummelo and the DH pummelo showed normal growth and produced many flowers and fruit. In this chapter, we describe about the morphological characteristics and the reproductive potential of the haploid pummelo and the DH pummelo.

  • 植物育種学辞典(共著)

    監修者:鵜飼保雄、大澤良、堤伸浩、長峰司、二宮正司、平田豊、藤垣順三、三位正洋共著者:國武久登ほか、日本育種学会関係者350名 (Part: Joint Work )

    培風館(東京)全785頁、分担 10語  2005.08 ISBN: 4-563-07788-7

  • Biotechnology in Agriculture and Forestry, Vol. 49 Somatic hybridization in crop improvement Ⅱ

    編集者:Bajaj, Y.P.S. 共著者:Kunitake, H., M. Mii (Part: Joint Work )

    Springer-Verlag (Germany)全377頁分担 95-111頁  2001.07

  • Biotechnology in Agrculture and Forestry, Vol. 34 Plant protoplasts and genetic engineering Ⅵ

    編集者:Bajaj, Y.P.S. 共著者:Takahata, Y., H. Jyomori, S. Miyano, H. Kunitake, M. Mii (Part: Joint Work )

    Springer-Verlag (Germany)全451頁分担 55-62頁  1995.12

  • Biotechnology in Agriculture and Forestry, Vol. 30 Somatic embryogenesis and synthetic seed Ⅰ

    編集者:Bajaj, Y.P.S. 共著者:Kunitake, H., M. Mii (Part: Joint Work )

    Springer-Verlag (Germany)全472頁分担 280-298頁  1995.02

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Review Papers 【 display / non-display

  • The phenomenon of root elongation and high respiration actibity in the rolB-gene-enhanced FSL#35 variant of Lotus corniculatus FOX-SR line.

    Yano Tsubasa, Yamamoto Akihiro, Kunitake Hisato, Saeki Yuichi, Akashi Ryo

    Plant Root   8 ( 0 ) 82 - 91   2014.08

    Introduction and explanation (bulletin of university, research institution)   Joint Work

    DOI CiNii

  • Comparison of cultivars and seasonal variation in blueberry (Vaccinium species) leaf extract on Adult T-cell leukemia cell line growth suppression.

    Kai Hisahiro, Fuse Takuichi, Kunitake Hisato, Morishita Kazuhiro and Matsuno Koji

    Medicines   1 ( 1 ) 3 - 11   2014.06

    Introduction and explanation (bulletin of university, research institution)   Joint Work


  • Comparison of cultivars and seasonal variation in blueberry (Vaccinium species) leaf extract on Adult T-cell leukemia cell line growth suppression.

    Kai Hisahiro, Fuse Takuichi, Kunitake Hisato, Morishita Kazuhiro and Matsuno Koji

    Medicines   1 ( 1 ) 3 - 11   2014.06

    Introduction and explanation (bulletin of university, research institution)   Joint Work

  • Gardening plants, Citrus species.

    Hisato Kunitake

      57 ( 4 ) 4 - 7   2007.04

    Introduction and explanation (commerce magazine)   Single Work

Presentations 【 display / non-display

  • アジア野生種を利用したブルーベリーの品種改良


    Modern Blueberry Breeding Techniques Global Collaboration Training Program  2018.12  -  2018.12  中国昆明市ブルーベリー研究所

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  • SSRマーカーによる半数体ブンタンの遺伝子型解析


    園芸学会平成29年度秋季大会  2017.09  -  2017.09  園芸学会

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  • みやだいCOC事業の事例紹介

    國武 久登

    平成28年度COC/COC+全国シンポジウム  2017.03  -  2017.03  高知大学

  • Effect of temperature on self-incompatibility in Citrus pistil and mature pollen culture systems.

    Kawano S., Kunitake H., Li. Y., Yahata M.

    The Third International Symposuim on Citrus Biotechnology  2014.11  -  2014.11  ISHS

  • Self-incompatibility-like response induces alteration of actin cytoskeleton and cytosolic calsium in the Citrus pollen tube of Banpeiyu and Hyuganatsu.

    Li Y., Fuse T., Kawano S. Kunitake H

    The Third International Symposium on Citrus Biotechnology   2014.11  -  2014.11  ISHS

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Preferred joint research theme 【 display / non-display

  • Production of new blueberry cultivars

  • Production of new kumquat cultivars

  • Production of new raspberry cultivars

  • Biotechnology of fruit crops