NISHINO Koichiro




Research Fields, Keywords

Epigenetics, iPS cells, Regenerative medicine

Mail Address

E-mail address

Laboratory Phone number


Laboratory Fax number


Graduating School 【 display / non-display


    Saitama University   Faculty of Science   Department of Regulatory Biology   Graduated

Graduate School 【 display / non-display


    The University of Tokyo    Animal Resource Sciences  Doctor's Course  Completed

Degree 【 display / non-display

  • The University of Tokyo -  Ph.D

Field of expertise (Grants-in-aid for Scientific Research classification) 【 display / non-display

  • Molecular biology

  • Cell biology

  • Developmental biology

  • Genetics/Chromosome dynamics

  • Epigenetics


Papers 【 display / non-display

  • Epigenetic-scale comparison of human iPSCs generated by retrovirus, Sendai virus or episomal vectors

    Nishino K, Arai Y, Takasawa k, Toyoda M, Yamazaki-Inoue M, Sugawara T, Akutsu H, Nishimura K, Ohtaka M, Nakanishi M, Umezawa A.

    Regenerative Therapy   9   71 - 78   2018.12  [Refereed]

    Joint Work

     View Summary

    Human induced pluripotent stem cells (iPSCs) are established by introducing several reprogramming factors, such as OCT3/4, SOX2, KLF4, c-MYC. Because of their pluripotency and immortality, iPSCs are considered to be a powerful tool for regenerative medicine. To date, iPSCs have been established all over the world by various gene delivery methods. All methods induced high-quality iPSCs, but epigenetic analysis of abnormalities derived from differences in the gene delivery methods has not yet been performed. Here, we generated genetically matched human iPSCs from menstrual blood cells by using three kinds of vectors, i.e., retrovirus, Sendai virus, and episomal vectors, and compared genome-wide DNA methylation profiles among them. Although comparison of aberrant methylation revealed that iPSCs generated by Sendai virus vector have lowest number of aberrant methylation sites among the three vectors, the iPSCs generated by non-integrating methods did not show vector-specific aberrant methylation. However, the differences between the iPSC lines were determined to be the number of random aberrant hypermethylated regions compared with embryonic stem cells. These random aberrant hypermethylations might be a cause of the differences in the properties of each of the iPSC lines.


  • DNA hypermethylation enhanced telomerase reverse transcriptase expression in human-induced pluripotent stem cells

    Takasawa K., Arai Y., Yamazaki-Inoue M., Toyoda M., Akutsu H., Umezawa A., Nishino K.

    Human Cell   31   78 - 86   2018.01  [Refereed]

    Joint Work

     View Summary

    During reprogramming into human induced pluripotent stem cells (iPSCs), several stem cell marker genes are induced, such as OCT-4, NANOG, SALL4, and TERT. OCT-4, NANOG, and SALL4 gene expression can be regulated by DNA methylation. Their promoters become hypomethylated in iPSCs during reprogramming, leading to their induced expression. However, epigenetic regulation of the TERT gene remains unclear. In this study, we focused on epigenetic regulation of the human TERT gene and identified a differentially methylated region (DMR) at a distal region in the TERT promoter between human iPSCs and their parental somatic cells. Interestingly, the TERT-DMR was highly methylated in iPSCs, but low-level methylation was observed in their parental somatic cells. Region-specific, methylated-promoter assays showed that the methylated TERT-DMR up-regulated the promoter activity in iPSCs. In addition, Lamin B1 accumulated at the TERT-DMR in iPSCs, but not in their parent somatic cells. These results suggested that the TERT transcription was enhanced by DNA methylation at the TERT-DMR via binding to nuclear lamina during reprogramming. Our findings shed light on a new functional aspect of DNA methylation in gene expression.

    DOI PubMed

  • The differentially DNA-methylated region responsible for expression of runt-related transcription factor 2

    Wakitani S., Wakitani S., Yokoi D., Hidaka Y., Nishino K.

    Journal of Veterinary Medical Science   79   230 - 237   2017.02  [Refereed]

    Joint Work

    DOI PubMed CiNii

  • DNA methylation dynamics in human induced pluripotent stem cells

    Nishino K., Umezawa A.

    Human Cell   29   97 - 100   2016.07  [Refereed]  [Invited]

    Joint Work

    DOI PubMed

  • Tetraploid Embryonic Stem Cells Maintain Pluripotency and Differentiation Potency into Three Germ Layers

    Imai H, Kano K, Fujii W, Takasawa K, Wakitani S, Hiyama M, Nishino K, Kusakabe KT, Kiso Y.

    PLoS ONE   10   e0130585   2015.06  [Refereed]

    Joint Work


display all >>

Review Papers 【 display / non-display

  • Induced Pluripotent Stem Cells from Human Extra-Embryonic Amnion Cells: Role of DNA Methylation in Maintaining Stemness

    Nishino K, Umezawa A

    Stem Cells and Cancer Stem Cells ( Springer )  4   59 - 65   2012.12

    Introduction and explanation (commerce magazine)   Joint Work

  • DNA methylation dynamics in induced pluripotent stem cells

      30 ( 10 ) 1556 - 1561   2012.06

    Introduction and explanation (scientific journal)   Joint Work

  • Extra-Embryonic Amnion Cells: Role of DNA Methylation in Maintaining Stemness

    1. Nishino K, Umezawa K

    Stem Cells and Cancer Stem Cells ( Springer )  4   59 - 65   2012.03

    Introduction and explanation (commerce magazine)   Joint Work

  • DNA methylation Dynamics in induced pluripotent stem cells

      239 ( 14 ) 1259 - 1264   2011.12

    Introduction and explanation (commerce magazine)   Joint Work

  • Regenerative medicine and Epigenetics

      28 ( 15 ) 2546 - 2551   2010.09

    Introduction and explanation (scientific journal)   Joint Work

Presentations 【 display / non-display

  • イヌSF-1遺伝子のmRNA全長配列の同定DNA methylation induced human telomerase reverse transcriptase gene expression via lamin B1

    関谷麻杜、高澤建、新井良和、西野光一郎Ken Takaswa, Yoshikazu Arai, Mayu Yamazaki-Inoue, Masashi Toyoda, Hidenori Akutsu, Akihiro Umezawa, Koichiro Nishino

    The 72th Fujihara seminar 2017  2017.09  -  2017.09  藤原科学財団

  • DNA methylation dynamics in human induced pluripotent stem cells over time

    Koichiro Nishino, Akihiro Umezawa

    慶応大学GCOEファイナルシンポジウム  2012.12  -  2012.12  慶応大学医学部GCOE

  • DNA methylation dynamics in human Embryonal Carcinoma cells

    2012.05  -  2012.05 

  • DNA methylation dynamics in human induced pluripotent stem cells over time

    2011.05  -  2011.05 

  • Defining hypo-methylated region of stem cell-specific promoters despite general hyper-methylation status in human iPS cells

    Koichiro Nishino, Masashi Toyoda, Mayu Yamazaki-Inoue, Hatsune Makino, Yoshihiro Fukawatase, Emi Chikazawa, Yoriko Takahashi, Hidenori Akutsu, Akihiro Umezawa

    Japan Society of Gene Therapy, The 16th Annual Meeting 2010  2010.07  -  2010.07  Japan Society of Gene Therapy

display all >>