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Affiliation |
Faculty of Agriculture Region of Veterinary Science |
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Associate Professor |
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MARUYAMA Keisuke
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Research Areas 【 display / non-display 】
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Life Science / Metabolism and endocrinology / 比較内分泌
Papers 【 display / non-display 】
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Distribution of neuromedin U (NMU)-like immunoreactivity in the goldfish brain, and effect of intracerebroventricular administration of NMU on emotional behavior in goldfish. Reviewed International journal
Kouhei Matsuda, Keisuke Watanabe, Yoshiki Miyagawa, Keisuke Maruyama, Norifumi Konno, Tomoya Nakamachi
Peptides 156 170846 - 170846 2022.10
Language：English Publishing type：Research paper (scientific journal)
Neuromedin U (NMU) is a multifunctional neuropeptide implicated in regulation of smooth muscle contraction in the circulatory and digestive systems, energy homeostasis and the stress response, but especially food intake in vertebrates. Recent studies have indicated the possible involvement of NMU in the regulation of psychomotor activity in rodents. We have identified four cDNAs encoding three putative NMU variants (NMU-21, -25 and -38) from the goldfish brain and intestine. Recently, we have also purified these NMUs and the truncated C-terminal form NMU-9 from these tissues, and demonstrated their anorexigenic action in goldfish. However, there is no information on the brain localization of NMU-like immunoreactivity and the psychophysiological roles of NMU in fish. Here, we investigated the brain distribution of NMU-like immunoreactivity and found that it was localized throughout the fore- and mid-brains. We subsequently examined the effect of intracerebroventricular (ICV) administration of NMU-21, which is abundant only in the brain on psychomotor activity in goldfish. As goldfish prefer the lower to the upper area of a tank, we developed an upper/lower area preference test in a tank for evaluating the psychomotor activity of goldfish using a personal tablet device without an automatic behavior-tracking device. ICV administration of NMU-21 at 10 pmol g-1 body weight (BW) prolonged the time spent in the upper area of the tank, and this action mimicked that of ICV administration of the central-type benzodiazepine receptor (CBR) agonist tofisopam at 100 pmol g-1 BW. These results suggest that NMU-21 potently induces anxiolytic-like action in the goldfish brain.
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Tanaka Y., Taguchi S., Maruyama K., Mori K., Miyazato M., Kangawa K., Murakami N., Nakahara K.
Biochemical and Biophysical Research Communications 534 653 - 658 2021.1
Language：English Publishing type：Research paper (scientific journal) Publisher：Biochemical and Biophysical Research Communications
© 2020 Elsevier Inc. Two novel peptides, neuromedin U precursor-related peptide (NURP) and neuromedin S precursor-related peptide (NSRP), are produced from neuromedin U (NMU) and neuromedin S (NMS) precursors, respectively, as these precursors have multiple consensus sequences for proteolytic processing. Our group has shown previously that one of these two novel peptides, NURP, stimulates body temperature and locomotor activity, but not food intake. However, the physiological function of the other peptide, NSRP, has remained unclear. Therefore, the aim of this study was to characterize differences in the regions of the rat brain targeted by the NMU/NMS peptide family, including NURP and NSRP, and their physiological functions. First, we explored the regions of c-Fos expression after intracerebroventricular (i.c.v.) injection of NURP and NSRP and found that these were fewer than after i.c.v. injection of NMU and NMS in the hypothalamus, possibly because NURP and NSRP cannot activate NMU/NMS receptors. In the ventral subiculum, which is one region of the hippocampus, c-Fos expression was evident only after i.c.v. injection of NURP. We also examined the effects of NSRP on food intake, body temperature and locomotor activity. Like NURP, NSRP increased both body temperature and locomotor activity, but not food intake, indicating that NSRP is also a functional peptide. However, these effects of NSRP were distinctly weaker than those of NURP. These findings suggest differences in the affinity of NURP and/or NSRP for specific receptors, or in their respective biological activities.
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Neuromedin U suppresses prolactin secretion via dopamine neurons of the arcuate nucleus. Reviewed
Nakahara K, Maruyama K, Ensho T, Mori K, Miyazato M, Kangawa K, Uemura R, Sakoda H, Nakazato M, Murakami N
Biochemical and biophysical research communications 521 ( 2 ) 521 - 526 2020.1
Language：English Publishing type：Research paper (scientific journal)
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Liver-expressed antimicrobial peptide 2 antagonizes the effect of ghrelin in rodents. Reviewed
Islam MN, Mita Y, Maruyama K, Tanida R, Zhang W, Sakoda H, Nakazato M
The Journal of endocrinology 244 ( 1 ) 13 - 23 2020.1
Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1530/JOE-19-0102
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Purification and identification of native forms of goldfish neuromedin U from brain and gut. Reviewed
Maruyama K, Kaiya H, Miyazato M, Murakami N, Nakahara K, Matsuda K
Biochemical and biophysical research communications 517 ( 3 ) 433 - 438 2019.9
Language：English Publishing type：Research paper (scientific journal)
Books 【 display / non-display 】
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肥満外科モデル動物の作製と意義 最新肥満症学
宮澤崇、丸山圭介、山下貴裕（ Role： Joint author）
日本臨牀社 2014.5
Language：Japanese
Presentations 【 display / non-display 】
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ニューロメジンS前駆体中の新規ペプチド（NSRP）の生理機能の探索
田口 史門、延生 卓也、森 健二、宮里 幹也、寒川 賢治、丸山 圭介、村上 昇、中原 桂子
第162回日本獣医学会学術集会 (茨城県つくば市 つくば国際会議場) 日本獣医学会
Event date： 2019.9.10 - 2019.9.12
Language：Japanese Presentation type：Oral presentation (general)
Venue：茨城県つくば市 つくば国際会議場
Grant-in-Aid for Scientific Research 【 display / non-display 】
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「生殖」と「摂食」の統合的制御における、NURPとNMUの生理的役割の解明
Grant number：25K09479 2025.04 - 2028.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
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スプライシングバリアントの探索を基盤とした、NURPとNMUの生理的役割の解明
Grant number：22K06045 2022.04 - 2026.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
Authorship：Principal investigator
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摂食・エネルギー代謝を制御する新規生理活性ペプチドの発見と応用
Grant number：21K05958 2021.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship：Coinvestigator(s)
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新規ペプチド"NURP"と"NSRP"のトランスレーショナルリサーチ
Grant number： 20H03153 2020.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
中原 桂子、
Authorship：Coinvestigator(s)
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NMUとの機能的連関に着目した、新規ペプチドホルモンNURPの生理機能の解明
Grant number：19K09009 2019 - 2022.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator