Graduating School 【 display / non-display


    University of Miyazaki   Faculty of Agriculture   Graduated

Graduate School 【 display / non-display


    University of Miyazaki  Graduate School, Division of Agricultural Science  Master's Course  Completed


    The University of Tokyo  Graduate School, Division of Agricultural Science  Doctor's Course  Completed

Field of expertise (Grants-in-aid for Scientific Research classification) 【 display / non-display

  • Veterinary medical science


Papers 【 display / non-display

  • Establishment of a novel diagnostic test for bovine leukaemia virus infection using direct filter PCR

    Daous H.E., Mitoma S., Elhanafy E., Thi Nguyen H., Thi Mai N., Hara A., Duangtathip K., Takezaki Y., Kaneko C., Norimine J., Sekiguchi S.

    Transboundary Emerging Diseases     2020.02  [Refereed]

    Joint Work

  • Meteorological factor affecting the risk of transmission of HPAI in Miyazaki, Japan

    Arikawa G., Fujii Y., Abe M., Mai N.T., Mitoma S., Notsu K., Nguyen H.T., Elhanafy E., Daous H.E., Kabali E., Norimine J., Sekiguchi S.

    Veterinary Record Open     2019.09  [Refereed]

    Joint Work

  • Slaughterhouse survey for detection of bovine viral diarrhea infection among beef cattle in Kyushu, Japan.

    Agah MA, Notsu K, El-Khaiat HM, Arikawa G, Kubo M, Mitoma S, Okabayashi T, Mekata H, Elhanafy E, El Daous H, Mai TN, Nguyen TH, Isoda N, Sakoda Y, Norimine J, Sekiguchi S

    The Journal of veterinary medical science     2019.08  [Refereed]

    Joint Work

     View Summary

    <p>Bovine viral diarrhea virus (BVDV) footprint has spread across the globe and is responsible for one of the most economically important diseases in cattle. In Japan, some regional surveillance and preventive measures to control bovine viral diarrhea (BVD) have been implemented. However, BVDV infection is poorly understood in cattle industries, and there is no systematic BVD surveillance system and control program. Kyushu is the center for raising beef cattle in Japan. Therefore, this study aimed to determine the BVDV infection using a slaughterhouse survey among beef cattle in Kyushu, Japan. A total of 1,075 blood samples were collected at two regional slaughterhouses in Miyazaki prefecture from December 2015 to June 2016. Antigen ELISA was used for detection of BVDV antigen in blood samples. Two samples showed positive results (2/1,075; 0.18%). BVDV RNA was extracted from positive blood samples; the sequence was determined and analyzed by the neighbor-joining method for construction of the phylogenetic tree. Phylogenetic analysis based on the 5'-UTR revealed that the two positive samples were grouped into the same subtype BVDV-1b in the BVDV-1 genotype, but the infected cattle belonged to two different farms. In conclusion, this is the first study to identify the presence of BVDV in a slaughterhouse survey in Kyushu. These findings suggest that a slaughterhouse survey is a useful tool for developing a surveillance system for monitoring infectious diseases in cattle.</p>

    DOI PubMed CiNii

  • Teat papillomatosis in dairy herds: First detection of bovine papillomavirus type 10 in China

    Zhu W., Yuan D., Norimine J., Gao N., Fan S., DU Y., Li F., Yang M., Hu S., Dong J.

    The Journal of veterinary medical science   81   1063 - 1066   2019.07  [Refereed]

    Joint Work

     View Summary

    Teat papillomatosis is one important infectious disease affecting cattle health and results in significant economic losses especially in the dairy industry. Although there is a large number of commercial cattle herds in China, limited information is available for molecular epidemiological investigation of bovine papillomaviruses (BPVs). In October 2017, an outbreak of teat papillomatosis occurred in the Shandong Province of China. Samples were collected and diagnosed with PCR, and 3 full-length viral genomes were amplified from tissue samples collected from 3 outbreak farms. Analysis results revealed that the outbreak was associated with BPV type 10. This is the first report of BPV-10 infection in China and will contribute to the molecular epidemiological study of the disease.

    DOI PubMed CiNii

  • An outbreak of strangles associated with a novel genotype of Streptococcus equi subspecies equi in donkeys in China during 2018

    Dong J., Gao N., Waller A., Cook F., Fan S., Yuan D., Du Y., Li F., Norimine J., Zhu W.

    Equine Veterinary Journal     2019.03  [Refereed]

    Joint Work

     View Summary

    © 2019 EVJ Ltd Background: Strangles is a highly contagious respiratory disease of equids caused by the bacterium Streptococcus equi subspecies equi. Objectives: To identify the cause of an outbreak of strangles that occurred on donkey farms within the Shandong Province of China and determine the prevalence of the disease. Study design: Cross-sectional. Methods: Samples were taken from clinically affected animals to measure the prevalence of strangles within the population of donkeys at six intensive farms in China and identify the SeM type of isolate recovered from affected animals. Diagnosis was confirmed by bacterial isolation, biochemical tests and PCR. Epidemiological data were analysed using Chi-square test and a Fisher's exact two-sided test. The SeM gene of S. equi isolates recovered from affected animals was determined and compared with the SeM database PubMLST-seM. Results: In July and August 2018, an outbreak of strangles occurred on six donkey farms within the Shandong Province of China. The overall prevalence of disease within the different donkey herds was 13.4%. Younger animals were worst affected with 40.3% (83/206) of donkey foals aged under 1 year exhibiting clinical signs compared with 12.5% (191/1525) of donkeys aged one to 2 years and 3.8% (17/442) of donkeys over 2 years of age. Analysis of SeM sequencing data identified that the farms were affected by the same strain of S. equi, SD201807, which contains the novel 136 allele of SeM. Main limitations: Healthy donkeys were not sampled in this study. Conclusions: The number of intensive donkey breeding farms in China has risen recently. The higher numbers of animals that are in closer proximity to one another raise the potential for the transmission of infectious diseases such as strangles. This is the first description of a strangles outbreak among donkey herds in China.


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Books 【 display / non-display

  • Feline Immunology and Immunodeficiency

    Mikami, T., J. Norimine, T. Miyazawa (Part: Joint Work )

    Oxford University Press  1995.04

Review Papers 【 display / non-display

  • The major histocompatibility complex of ruminants.

    Amills M., V. Ramiya, J. Norimine, and H. A. Lewin.

    Rev. Sci. Tech.     1998  [Refereed]  [Invited]

    Introduction and explanation (scientific journal)   Joint Work

Other external funds procured 【 display / non-display

  • Analysis of antigen-specific CD4T cell responses against foot-and-mouth disease virus using bovine MHC II tetramer

    Project Year: 2017.04  -  2019.03 

Presentations 【 display / non-display

  • Tetramer-guided epitope mapping of FMDV capsid to evaluate CD4+T-cell responses following vaccination

    Mitoma S., Carr V., Moffat K., Harvey Y., Charleston B., Norimine J., Seago J.  [Invited]

    GFRA Scientific Meeting 2019  2019.10  -  2019.10 

  • Direct filter PCR as a novel diagnostic test for bovine leukemia virus infection

    Daous H.E.、三苫修也、Elhanafy E.、NgyenThi H.、Mai T.N.、原彰宏、Duangtathip K.、竹崎由佳、兼子千穂、乗峰潤三、関口敏

    第162回日本獣医学会学術集会  2019.09  -  2019.09 

  • Development of pooled testing system for porcine epidemic diarrhea using real-time fluorescent reverse-transcription loop-mediated isothermal amplification assay

    Mai T.N., Nguyen V.D., 山崎渉、岡林環樹、三苫修也、野津昂亮、山口良二、乗峰潤三、関口敏

    第162回日本獣医学会学術集会  2019.09  -  2019.09 

  • The role of BoLA-DRB3 alleles on disease progression during bovine leukemia virus infection

    Norimine J., Hayashi T., Mitoma S., Mekata H., Sekiguchi S.  [Invited]

    HTLV 19th International conference on human retrovirology  2019.04  -  2019.04 

  • Diversity of MHC-B locus in Vietnamese native chicken.

    Thi Huyen Nguyen, 三苫修也, 関口敏, 乗峰潤三

    平成30年第161回日本獣医学会学術集会(茨城県)  2018.09  -  2018.09 

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