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Faculty of Medicine School of Medicine Department of Anatomy, Histochemistry and Cell Biology |
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Assistant Professor |
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Related SDGs |
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PHYU Synn oo
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Papers 【 display / non-display 】
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Oo Phyu Synn, Yamaguchi Yuya, Tin Htwe Kyaw Myat, Noor Ali Mohmand, Choijookhuu Narantsog, Sawaguchi Akira, Hino Shin-ichiro, Srisowanna Naparee, Hishikawa Yoshitaka
ACTA HISTOCHEMICA ET CYTOCHEMICA 51 ( 1 ) 21 - 31 2018
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
Estrogen affects mitochondrial function in various tissues, but the precise mechanism remains unclear. We, therefore investigated the effect on estrogen-regulated mitochondrial morphology by dynamin-related protein 1 (Drp1) and its Ser616-phosphorylated derivative (pDrp1<sup>Ser616</sup>) are involved in mitochondrial fission. MCF7 human breast cancer cells were treated with 17β-estradiol (E<sub>2</sub>), an estrogen receptor (ER) α and β antagonist (ICI 182, 780), an ERα antagonist (MPP), and an ERβ antagonist (PHTPP) for 24 hr. The expression of Drp1 and pDrp1<sup>Ser616</sup> was analyzed by western blotting and immunohistochemistry. Mitochondrial morphology was analyzed by transmission electron microscopy (TEM). In control cells, Drp1 was detected in the cytoplasm of all cells while pDrp1 was observed in the cytoplasm of 3.4 ± 1.0% of the total population. After E<sub>2</sub> treatment, pDrp1<sup>Ser616</sup>-positive cells comprised 30.6 ± 5.6% of the total population, 10.5 ± 1.7% after E<sub>2</sub> + ICI treatment, 12.4 ± 4.2% after E<sub>2</sub> + MPP treatment, and 24.0 ± 2.2% after E<sub>2</sub> + PHTPP treatment. In ERα knockdown MCF7 cells, pDrp1 expression was decreased after E<sub>2</sub> treatment compared to E<sub>2</sub>-treated wild type cells. Tubular pattern mitochondria were found in the control cells but the number of short and small pattern mitochondria (< 0.5 μm<sup>2</sup>) was significantly increased after E<sub>2</sub> treatment (as observed by TEM). We, therefore concluded that the phosphorylation of Drp1 is important for E<sub>2</sub>-dependent mitochondrial morphological changes through ERα.
DOI： 10.1267/ahc.17034
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Kyaw M.T., Oo P.S., Sumera A., Sivakumar A., Santiago C., Aung Y.N.
International Journal of Hematology Oncology and Stem Cell Research 19 ( 3 ) 261 - 285 2025
Language：English Publishing type：Research paper (scientific journal) Publisher：International Journal of Hematology Oncology and Stem Cell Research
The combined use of plant-based antioxidants and iron chelators presents a synergistic treatment approach that effectively tackles both iron overload and the accompanying oxidative stress in individuals with transfusion-dependent Thalassemia (TDT). Plant-based antioxidants counteract reactive oxygen species (ROS) and oxidative damage, whereas iron chelators effectively bind excess iron, reducing the body's iron concentration. This combined therapy can be beneficial in improving TDT patients with iron overload. We systematically reviewed the literature exploring the plant-based antioxidants with iron chelators for iron overload in transfusion-dependent Thalassemia Patients. All fourteen included studies were randomized clinical trials, employing various randomization methods including simple randomization, double-blinded, triple-blinded, and crossover designs. The included studies enrolled participants across different age groups, including both young and adult patients. Despite the variability in plant-based antioxidants with iron-chelating properties, the key findings were as follows: Nine studies reported a significant reduction in iron overload, eight studies observed a marked decrease in oxidative stress markers, and five studies demonstrated reduced liver enzyme levels, suggesting potential hepatoprotective effects. All included studies reported significant effects of various supplements on key biomarkers, including total iron (Fe), ferritin, total iron-binding capacity (TIBC), total antioxidant capacity (TAC), malondialdehyde (MDA), and liver enzymes (AST, ALT). Silymarin, green tea, and grape seed extract (GSE) supplementation demonstrated notable reductions in total Fe, Ferritin, ASL, and ALT levels. Additionally, these supplements increased TIBC levels, suggesting improved iron metabolism. In contrast, quercetin and curcumin supplementation did not show a statistically significant difference compared to control groups in these outcomes.
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Natural fixatives alternative to formalin in histopathology: A systematic review Reviewed
Lum A.Y.W., Oo P.S., Aye S.N., Wei-Jet L., Xian V.C.C., Krishnappa P.
Medical Journal of Malaysia 78 ( 1 ) 98 - 108 2023.1
Authorship：Corresponding author Publishing type：Research paper (scientific journal) Publisher：Medical Journal of Malaysia
Introduction: Since constant long-term exposure to formaldehyde endangers the health of laboratory personnel, sugar-based natural products have become interesting alternative fixatives to formaldehyde because of their preservative and antibacterial properties. However, there are controversial findings on the fixative effects of natural fixatives. This study systematically reviews the evidence comparing natural fixatives’ types, dilutions, fixative properties and staining quality in normal tissues and histopathological specimens. Materials and methods: A comprehensive search was performed for studies comparing the natural fixatives- and formaldehyde-fixed tissues using databases from inception to January 2022: PubMed, Ovid Medline and Google Scholar. Two independent reviewers did data extraction. The data were pooled for the type of natural fixatives, their concentrations and fixative qualities compared to formaldehyde. Results: Fifteen studies were included in this systematic review. Nine studies used one natural fixative with different dilutions, while six used several natural fixatives to compare their fixative properties with formaldehyde. The most used natural fixative was honey (n = 12) followed by jaggery (n = 8), sugar (n = 3) and others (n = 1). Honey showed the most promising results in fixation and staining, which are compatible with formalin. Jaggery and sugar also showed the possibility of replacing formaldehyde in tissue fixation and staining in smaller tissue samples. Conclusion: Natural fixatives showed promising results in tissue fixation. However, optimising the concentrations and conditions of natural fixatives is difficult because of the different chemical constituents and production steps. More comprehensive studies are necessary for application.
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Antimicrobial Effects of Quebrachitol: A Systematic Review. Reviewed
Doris Evelyn Yah Hui Jong, Siang Yin Lee, Yun Khoon Liew, Phyu Synn Oo, Amar Harris Arifin, Zi Ni Ngai, Beek Yoke Chin, Shamala Salvamani and Rhun Yian Koh
Microbiology Research 17 ( 3 ) 52 2026.2
Language：English Publishing type：Research paper (scientific journal)
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Limitation of cane sugar solution as a tissue fixative for glycogen preservation in mouse liver Reviewed
Babar I.M., Oo P.S., Cheoh J.S., Aye L.M., Kyaw M.T., Aye S.N., Krishnappa P.
Medical Journal of Malaysia 80 ( 4 ) 403 - 409 2025.7
Publishing type：Research paper (scientific journal) Publisher：Medical Journal of Malaysia
Introduction: Sugar-based natural fixatives have been proposed as substitutes for formalin in histopathological practice because of their promising results in preserving tissue structures. However, they have not yet been applied. This study aimed to determine the optimal concentration of cane sugar solution (CSS) for tissue fixation, histochemical staining, and glycogen storage in mouse liver tissues. Materials and Methods: A total of 24 mouse liver tissues from six mice were divided into four groups and fixed with 10% neutral buffered formalin (NBF), 30%, 50% and 70% CSS, respectively, for 24 h at room temperature. After tissue processing and sectioning, the samples were stained with hematoxylin and eosin (H&E), methyl green pyronin Y (MGPY) and peroxidase acid-Schiff (PAS). The tissue sections were evaluated under a light microscope by two blinded pathologists. Data were analyzed using one-way analysis of variance (ANOVA) and a post-hoc test to assess the differences among the experimental groups. Results: The gross morphology of all the samples showed minimal shrinkage without color changes. Microscopic examination revealed that the 50% and 70% CSS groups showed comparable efficacy in cytoplasmic and nuclear staining, H&E staining intensity, deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) staining, and collagen staining with the 10% NBF group. The 30 percent CSS group showed significantly poorer nuclear and DNA staining than the 10% NBF group. All CSS groups failed to preserve glycogen in mouse liver tissues (p < 0.001) compared with the 10% NBF. Conclusions: Our study concluded that 50% and 70% CSS are potentially replaceable, eco-friendly fixatives for 10% NBF in small tissue samples for tissue fixation and histochemical staining. However, 10% NBF was still superior to CSS for glycogen preservation in mouse liver tissue.
MISC 【 display / non-display 】
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The role of estrogen receptors in intestinal homeostasis and disease.（共著）
Choijookhuu N, Hino SI, Oo PS, Batmunkh B, Hishikawa Y
Receptors & Clinical Investigation 3 ( 1 ) 1 - 8 2016.1
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher： Smart Science & Technology LLC
DOI： 10.14800/rci.1109
Presentations 【 display / non-display 】
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Estrogen regulates mitochondrial morphology through mitochondrial elongation factor (MIEF1) in human breast cancer cells International conference
Second Myanmar-Japan Joint Symposium
Event date： 2015.12.5 - 2015.12.6
Language：English Presentation type：Oral presentation (general)
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Mitochondrial elongation factor (MIEF1) regulates mitochondrial morphology t hrough estrogen in MCF7
Event date： 2015.9.18 - 2015.9.19
Language：English Presentation type：Oral presentation (general)
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マウス消化管におけるWWP2ユビキチンリガーゼの発現検討
日野真一郎,Choijookhuu Narantsog,Phyu Synn Oo,菱川善隆,
第47回日本臨床分子形態学会総会・学術集会 (長崎市) 日本臨床分子形態学会
Event date： 2015.9.18 - 2015.9.19
Language：Japanese Presentation type：Oral presentation (general)
Venue：長崎市
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Effects of estrogen on mitochondrial elongation through MIEF1 in human breast cancer cell line
Event date： 2015.3.21 - 2015.3.23
Language：English Presentation type：Poster presentation
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Mitochondria localization and morphology may be regulated by mitochondrial elongation factor 1 (MIEF1) through estrogen in MCF7
Phyu Synn Oo,菱川善隆
日本解剖学会第70回九州支部学術集会 (福岡県北九州市) 日本解剖学会
Event date： 2014.10.25
Language：English Presentation type：Oral presentation (general)
Venue：福岡県北九州市
Other research activities 【 display / non-display 】
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Assessment and possible implications in multiple infections and their related cancers in Myanmar
2016.01
ミャンマー国保健省医学研究局と宮崎大学との保健医療関連分野におけるシンポジウムに参加した。
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4th International Arsenic Symposium in Miyazaki
2015.10
宮崎大学でのヒ素研究成果について討論・情報交換をした。
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The9th International Conference on Genetic and Evolutionary Computing(ICGEC 2015)
2015.08
最新の技術について情報を得た。
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第40回組織細胞化学講習会
2015.08
「in situ ハイブリダイゼーションの基礎と応用ー実践編ー 」wet labを行った。
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Kick-Off Symposium on Promoting Environmental Health in Arsenic Contaminated Area in Myanmar
2015.08