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医学部 医学科 感覚運動医学講座眼科学分野 |
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助教 |
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Yamaguma Y., Sugita N., Choijookhuu N., Yano K., Lee D., Ikenoue M., Fidya , Shirouzu S., Ishizuka T., Tanaka M., Yamashita Y., Chosa E., Taniguchi N., Hishikawa Y.
Histochemistry and Cell Biology 157 ( 3 ) 359 - 369 2022年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Histochemistry and Cell Biology
High-mobility group box 2 (HMGB2) is a chromatin-associated protein that is an important regulator of gene transcription, recombination, and repair processes. The functional importance of HMGB2 has been reported in various organs, including the testis, heart, and cartilage. However, its role in the ovary is largely unknown. In this study, ovary tissues from wild-type (WT) and HMGB2-knock-out (KO) mice were examined by histopathological staining and immunohistochemistry. The ovary size and weight were significantly lower in HMGB2-KO mice than in age-matched WT littermates. Histopathological analysis revealed ovarian atrophy and progressive fibrosis in 10-month-old HMGB2-KO mouse ovaries. Compared to age-matched WT mice, the numbers of oocytes and developing follicles were significantly decreased at 2 months of age and were completely depleted at 10 months of age in HMGB2-KO mice. Immunohistochemistry revealed the expression of HMGB2 in the granulosa cells of developing follicles, oocytes, some corpora lutea, and stromal cells. Importantly, HMGB2-positive cells were co-localized with estrogen receptor beta (ERβ), but not ERα. Estrogen response element-binding activity was demonstrated by southwestern histochemistry, and it was decreased in HMGB2-KO mouse ovaries. Cell proliferation activity was also decreased in HMGB2-KO mouse ovaries in parallel with the decreased folliculogenesis. These results indicated that the depletion of HMGB2 induced ovarian atrophy that was characterized by a decreased ovarian size and weight, progressive fibrosis, as well as decreased oocytes and folliculogenesis. In conclusion, we demonstrated the crucial role of HMGB2 in mouse ovarian folliculogenesis through ERβ expression.
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Sugita N, Choijookhuu N, Yano K, Lee D, Ikenoue M, Fidya, Taniguchi N, Chosa E, Hishikawa Y
Biology of reproduction 105 ( 6 ) 1510 - 1520 2021年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Biology of reproduction
High-mobility group box 2, a chromatin-associated protein that interacts with deoxyribonucleic acid, is implicated in multiple biological processes, including gene transcription, replication, and repair. High-mobility group box 2 is expressed in several tissues, including the testis; however, its functional role is largely unknown. Here, we elucidated the role of high-mobility group box 2 in spermatogenesis. Paraffin-embedded testicular tissues were obtained from 8-week-old and 1-year-old wild-type and knock-out mice. Testis weight and number of seminiferous tubules were decreased, whereas atrophic tubules were increased in high-mobility group box 2-depleted mice. Immunohistochemistry revealed that atrophic tubules contained Sertoli cells, but not germ cells. Moreover, decreased cell proliferation and increased apoptosis were demonstrated in high-mobility group box 2-depleted mouse testis. To elucidate the cause of tubule atrophy, we examined the expression of androgen and estrogen receptors, and the results indicated aberrant expression of androgen receptor and estrogen receptor alpha in Sertoli and Leydig cells. Southwestern histochemistry detected decreased estrogen response element-binding sites in high-mobility group box 2-depleted mouse testis. High-mobility group box 1, which has highly similar structure and function as high-mobility group box 2, was examined by immunohistochemistry and western blotting, which indicated increased expression in testis. These findings indicate a compensatory increase in high-mobility group box 1 expression in high-mobility group box 2 knock-out mouse testis. In summary, depletion of high-mobility group box 2 induced aberrant expression of androgen receptor and estrogen receptor alpha, leading to decreased germ cell proliferation and increased apoptosis which resulted in focal seminiferous tubule atrophy.
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脳動脈瘤クリッピング手術後に眼窩先端症候群を呈した1例 査読あり
日高 貴子, 中馬 秀樹, 杉田 直大, 直井 信久
眼科臨床紀要 7 ( 4 ) 271 - 275 2014年4月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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部分視神経低形成の診断から20年後に正常眼圧緑内障を発症した1例 査読あり
杉田 直大, 中馬 秀樹, 河野 尚子, 尾崎 峯生, 直井 信久
眼科臨床紀要 6 ( 12 ) 969 - 974 2013年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
講演・口頭発表等 【 表示 ／ 非表示 】
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Scedosporium Apiaspermumによる感染性強膜炎の1例
杉田直大
第5回九州角膜フォーラム
開催年月日： 2019年10月19日
記述言語：日本語 会議種別：口頭発表（一般）
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Scedosporium Apiaspermumによる感染性強膜炎の1例
杉田直大
第5回九州角膜フォーラム
開催年月日： 2019年10月19日
記述言語：日本語 会議種別：口頭発表（一般）
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Bacillus cererusをPCRにて早期に同定でき、治療が奏功した眼内異物外傷後眼内炎の症例報告
菅井洸，児玉悠，杉田直大，中山恵介，馬渡剛，直井信久
第89回九州眼科学会
開催年月日： 2019年5月31日 - 2019年6月2日
記述言語：日本語 会議種別：口頭発表（一般）
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Bacillus眼内炎に対し治療が奏功した一例
菅井洸，児玉悠，杉田直大，中山恵介，馬渡剛，直井信久
第83回宮崎大学眼科研究会
開催年月日： 2019年3月16日
記述言語：日本語 会議種別：口頭発表（一般）
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認知症症例に生じた原発急性閉塞隅角緑内障に前房穿刺が奏功した1例
杉田直大，中馬秀樹，日髙貴子，直井信久
第34回九州緑内障カンファレンス (長崎県長崎市)
開催年月日： 2018年5月25日 - 2018年5月27日
記述言語：日本語 会議種別：口頭発表（一般）
開催地：長崎県長崎市