岡林 環樹 (オカバヤシ タマキ)

OKABAYASHI Tamaki

写真a

所属

産業動物防疫リサーチセンター

職名

教授

外部リンク

学位 【 表示 / 非表示

  • 獣医学 ( 2000年3月   酪農学園大学 )

 

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  • Development and validation of indirect ELISA for antibody detection against different protein antigens of porcine epidemic diarrhea virus in the colostrum and milk of sows

    Srijangwad A., Tripipat T., Saeng-chuto K., Jermsujarit P., Tantituvanont A., Okabayashi T., Nilubol D.

    Journal of Immunological Methods   494   113045   2021年7月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Journal of Immunological Methods  

    The objectives of this study are to develop and optimize indirect ELISA based on three coating antigens of porcine epidemic diarrhea virus (PEDV), recombinant spike (S12), nucleocapsid (N), and whole viral (WV) proteins, for the detection of IgG and IgA antibodies in colostrum and milk and to evaluate the diagnostic sensitivity (DSe) and diagnostic specificity (DSp) of the assay as a diagnostic method. Colostrum (n = 347) and milk (n = 272) samples from sows were employed in this assay. Indirect ELISA based on three coating antigens was assessed by receiver operating characteristic (ROC) curve analysis with a virus neutralization (VN) test as a reference method, and the cutoff value for calculating DSe and DSp was determined. S12-ELISA showed higher DSe and DSp of IgG and IgA detection compared to N- and WV-ELISA in both colostrum and milk samples. Moreover, S12-ELISA showed perfect agreement and a high correlation with the VN test, which was better than the N- and WV-ELISA for both IgG and IgA detection in colostrum and milk. In contrast, N-ELISA showed lower DSe and DSp compared to S12- and WV-ELISA, along with a correlation with VN and substantial agreement with the VN test. Nevertheless, our developed ELISAs have accuracy for repeatability in both inter- and intra-assay variation. Overall, this research demonstrates that S12-ELISA is more suitable than WV- and N-ELISA to detect IgG and IgA antibodies against PEDV from both colostrum and milk samples.

    DOI: 10.1016/j.jim.2021.113045

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    PubMed

  • Bovine respiratory coronavirus enhances bacterial adherence by upregulating expression of cellular receptors on bovine respiratory epithelial cells

    Fahkrajang W., Sudaryatma P.E., Mekata H., Hamabe S., Saito A., Okabayashi T.

    Veterinary Microbiology   255   109017   2021年4月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Veterinary Microbiology  

    Bovine coronavirus (BCoV) is one of the agents causing bovine respiratory disease complex (BRDC), with single infection tending to be mild to moderate; the probability of developing pneumonia in BRDC may be affected by viral and bacterial combinations. Previously, we reported that bovine respiratory syncytial virus (BRSV) infection enhances adherence of Pasteurella multocida (PM) to cells derived from the bovine lower respiratory tract but that BRSV infection in cells derived from the upper respiratory tract reduces PM adherence. In this study, we sought to clarify whether the modulation of bacterial adherence to cells derived from the bovine upper and lower respiratory tract is shared by other BRDC-related viruses by infecting bovine epithelial cells from the trachea, bronchus and lung with BCoV and/or PM. The results showed that cells derived from both the upper and lower respiratory tract were susceptible to BCoV infection. Furthermore, all cells infected with BCoV exhibited increased PM adherence via upregulation of two major bacterial adhesion molecules, intercellular adhesion molecule-1 (ICAM-1) and platelet-activating factor receptor (PAF-R), suggesting that compared with BRSV infection, BCoV infection differentially modulates bacterial adherence. In summary, we identified distinct interaction between bovine respiratory viruses and bacterial infections.

    DOI: 10.1016/j.vetmic.2021.109017

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    PubMed

  • Identification of Escherichia coli and related enterobacteriaceae and examination of their phenotypic antimicrobial resistance patterns: A pilot study at a wildlife-livestock interface in Lusaka, Zambia

    Kabali E., Pandey G.S., Munyeme M., Kapila P., Mukubesa A.N., Ndebe J., Muma J.B., Mubita C., Muleya W., Muonga E.M., Mitoma S., Hang’ombe B.M., Wiratsudakul A., Ngan M.T., Elhanafy E., El Daous H., Huyen N.T., Yamazaki W., Okabayashi T., Abe M., Norimine J., Sekiguchi S.

    Antibiotics   10 ( 3 )   1 - 17   2021年3月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Antibiotics  

    A cross-sectional study was used to identify and assess prevalence and phenotypic antimicrobial resistance (AMR) profiles of Escherichia coli and other enterobacteria isolated from healthy wildlife and livestock cohabiting at a 10,000 acres game ranch near Lusaka, Zambia. Purposive sampling was used to select wildlife and livestock based on similarities in behavior, grazing habits and close interactions with humans. Isolates (n = 66) from fecal samples collected between April and August 2018 (n = 84) were examined following modified protocols for bacteria isolation, biochemical identification, molecular detection, phylogenetic analysis, and antimicrobial susceptibility testing by disc diffusion method. Data were analyzed using R software, Genetyx ver.12 and Mega 6. Using Applied Profile Index 20E kit for biochemical identification, polymerase chain reaction assay and sequencing, sixty-six isolates were identified to species level, of which Escherichia coli (72.7%, 48/66), E. fergusonii (1.5%, 1/66), Shigella sonnei (22.7%, 14/66), Sh. flexinerri (1.5%, 1/66) and Enterobacteriaceae bacterium (1.5%, 1/66), and their relationships were illustrated in a phylogenetic tree. Phenotypic antimicrobial resistance or intermediate sensitivity expression to at least one antimicrobial agent was detected in 89.6% of the E. coli, and 73.3% of the Shigella isolates. The E. coli isolates exhibited the highest resistance rates to ampicillin (27%), ceftazidime (14.3%), cefotaxime (9.5%), and kanamycin (9.5%). Multidrug resistance (MDR) was detected in 18.8% of E. coli isolates while only 13.3% Shigella isolates showed MDR. The MDR was detected among isolates from impala and ostrich (wild animals in which no antimicrobial treatment was used), and in isolates from cattle, pigs, and goats (domesticated animals). This study indicates the possible transmission of drug-resistant microorganisms between animals cohabiting at the wildlife-livestock interface. It emphasizes the need for further investigation of the role of wildlife in the development and transmission of AMR, which is an issue of global concern.

    DOI: 10.3390/antibiotics10030238

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  • Metagenomic identification, sequencing, and genome analysis of porcine hepe-astroviruses (bastroviruses) in porcine feces in Japan.

    Nagai M, Okabayashi T, Akagami M, Matsuu A, Fujimoto Y, Hashem MA, Mekata H, Nakao R, Matsuno K, Katayama Y, Oba M, Omatsu T, Asai T, Nakagawa K, Ito H, Madarame H, Kawai K, Ito T, Nonaka N, Tsukiyama-Kohara K, Inoshima Y, Mizutani T, Misawa N

    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases   88   104664   2021年3月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.meegid.2020.104664

    PubMed

  • Isolation of Severe Fever with Thrombocytopenia Syndrome Virus from Various Tick Species in Area with Human Severe Fever with Thrombocytopenia Syndrome Cases.

    Sato Y, Mekata H, Sudaryatma PE, Kirino Y, Yamamoto S, Ando S, Sugimoto T, Okabayashi T

    Vector borne and zoonotic diseases (Larchmont, N.Y.)   2021年2月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/vbz.2020.2720

    PubMed

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書籍等出版物 【 表示 / 非表示

  • 獣医微生物学 第4版

    関崎 勉 他( 担当: 共著)

    文永堂  2018年7月 

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    記述言語:日本語 著書種別:教科書・概説・概論

MISC 【 表示 / 非表示

  • 産業動物とコロナウイルス感染について 招待あり

    岡林 環樹

    獣医公衆衛生研究   23   41 - 44   2021年4月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)   出版者・発行元:全国公衆衛生獣医師協議会  

    新型コロナウイルス感染症COVID-19と呼ばれる重症急性呼吸器症候群(SARS)関連コロナウイルス-2型(SARS-CoV-2)の発生により、「コロナウイルス」が一躍世界的に有名になった。これは新しい病原体なのか?ヒト以外の動物にも、その名の通りの重篤な呼吸器疾患を起こすのか?
    産業動物におけるコロナウイルスは、その感染拡大の早さによる蔓延化および環境中に排泄されたウイルスによる常在化を特徴とするために、経済的被害の大きい感染症を引き起こすことが認識されている。ウシにおけるコロナウイルスは下痢を主徴とする感染症の原因として知られているが、呼吸器疾患との関連性も明らかとなってきている。ブタにおけるコロナウイルスは、ブタ伝染性胃腸炎、ブタ流行性下痢など哺乳豚に致死的な下痢疾患を引き起こし、養豚業に壊滅的なダメージを与えている。ニワトリにおけるコロナウイルスは、ニワトリ伝染性気管支炎という監視届出伝染病の病原体として知られている。本病はウイルスの血清型の多様さによるワクチン問題や、その他の病原体との複合感染による重症化問題が浮き彫りとなっている。
    ヒト医学領域におけるコロナウイルスの位置付けは、「鼻風邪の原因ウイルスの一つ」程度の認識でありワクチンなども開発されず、その詳細は不明であった。しかし、21世紀に入ってからの、中国を起源とするSARSコロナウイルス、中東を起源とする中東呼吸器症候群コロナウイルスの流行、そして現在も世界的猛威を振るうSARS-CoV-2、という3つの重症肺炎感染症が問題起こった。幸か不幸か、これらの世界的な流行がコロナウイルスの認識のみならず、その研究を活性化させ、新たな知見が集積されてきている。これらのヒトに重症肺炎感染症を起こすコロナウイルスがコウモリ、ハクビシン、ラクダを介して、ヒトへの感染を拡大したと考えられている。そこで本稿においては、獣医学領域、特に産業動物におけるコロナウイルスに着目し、コロナウイルスによる感染症、種を超えた伝播に関する知見、そしてSARS-CoV-2との関連性について概説する。

  • Pseudorabies virus infection in hunting dogs in oita, japan: Report from a prefecture free from aujeszky’s disease in domestic pigs

    Kaneko C., Kaneko Y., Sudaryatma P.E., Mekata H., Kirino Y., Yamaguchi R., Okabayashi T.

    Journal of Veterinary Medical Science   83 ( 4 )   680 - 684   2021年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(大学・研究所紀要)   出版者・発行元:Journal of Veterinary Medical Science  

    We isolated two pseudorabies virus (PRV) isolates (designated OT-1 and OT-2) from two hunting dogs exhibiting neurological manifestations after eating the flesh of wild boar hunted in Oita prefecture, Kyushu Island, Japan. The isolates corresponded to a previously reported PRV (MY-1 strain) isolated from a hunting dog in neighboring Miyazaki prefecture, and it clustered into genotype II based on the glycoprotein C sequence. Our results suggest that this common PRV strain may have been maintained in wild boars on Kyushu Island even though domestic pigs in this area have attained an Aujeszky’s disease-free status.

    DOI: 10.1292/jvms.20-0450

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    CiNii Article

  • 動物におけるコロナウイルス感染症  招待あり

    岡林 環樹

    家畜感染症学会誌   9 ( 2 )   31 - 38   2020年7月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)   出版者・発行元:家畜感染症学会誌  

  • 伴侶動物から感染する人獣共通感染症 招待あり

    岡林 環樹

    現代化学   2020年7月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)   出版者・発行元:東京化学同人  

  • 宮崎大学CADIC アフリカ豚コレラ緊急セミナー報告 招待あり

    岡林 環樹

    養豚界   ( 1月号 )   56 - 58   2019年1月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)   出版者・発行元:緑書房  

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  • SFTS流行地 ”宮崎” からの報告 招待あり

    岡林 環樹

    令和3年度獣医学術九州地区学会教育講演  2021年10月22日 

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    開催年月日: 2021年10月22日 - 2021年10月31日

    記述言語:日本語   会議種別:口頭発表(招待・特別)  

  • Zoonotic Lessons from SARS-CoV-2: Emergence, Transmission, and Potential Therapeutic for the COVID-19 Pandemic Associated with the Human-Animal-Environment Interaction 招待あり 国際会議

    Tamaki Okabayashi

    2nd International Conference on Veterinary and Animal Science  2021年10月21日 

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    開催年月日: 2021年10月21日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

  • 国内外の大学連携による家畜防疫に関する知の集積 招待あり

    岡林 環樹

    第164回日本獣医学会学術集会  2021年9月9日 

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    開催年月日: 2021年9月7日 - 2021年9月13日

    会議種別:口頭発表(招待・特別)  

  • MOLECULAR MECHANISMS FOR ENHANCEMENT OF BACTERIAL ATTACHMENT TO BOVINE RESPIRATORY EPITHELIAL CELLS BY VIRUS INFECTION 国際会議

    PUTU EKA SUDARYATMA, TAMAKI OKABAYASHI

    Association of Japan-Indonesia Veterinary Education   (Bogor, Indonesia)  IPB University

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    開催年月日: 2021年2月13日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Bogor, Indonesia  

    Bovine respiratory disease complex (BRDC) is one of the most common and costly diseases in the cattle industry worldwide due to decreased production and increased costs associated with treatment. BRDC known as multiple infections by viruses and bacteria is considered to increase disease severity. Bovine RS virus (BRSV) and bovine coronavirus (BCoV) are the viral-pathogens causing BRDC. Pasteurella multocida (PM) was the most common bacterial agent paired with virus in BRDC. To investigate the interaction between bovine respiratory viruses and bacteria in bovine respiratory epithelial cells, we generated respiratory epithelial cell lines derived from bovine trachea (bTEC), bronchus (bBEC), and lung (bLEC). These established cells were infected with BRSV or BCoV. After then, we inoculated PM to these viral infected cells. BRSV infection significantly increased PM adherence to bovine lower respiratory tract epithelial cells, but not upper respiratory tract epithelial cells. All the cells infected with BCoV increased adherence of PM. These results indicate that BCoV infection in the upper and lower respiratory tracts enhance adherence of bacteria, and this phenomenon was markedly different from the BRSV infection. To investigate whether virus infection regulates any cellular adherence receptors on bovine epithelial cells, we performed proteomic and functional analyses. The proteomic analysis showed that BRSV infection decreased expression of intercellular adhesion molecule-1 (ICAM1) on bTECs. However, BRSV infection increased the accumulation of platelet-activating factor receptor (PAFR) in all cell types. Molecular experiments, including specific blockade, knockdown, and overexpression of these receptors, indicated that PM adherence to these cell types depended on PAFR expression. These data suggested that while bTECs possibly captures PM under normal condition, BRSV infection reverses this phenomena. To examine the interaction between BCoV infection and PM adherence in bovine upper and lower respiratory tract, epithelial cells were infected with BCoV and/or PM. The cells derived from both the upper and lower respiratory tract were susceptible to BCoV infection. These PM adherences are affected by increased expression of ICAM1 and PAFR. We have obtained evidence suggesting that the upper respiratory tract is responsible for inhibiting PM invasion of the lower respiratory tract. This inhibition is accomplished by trapping PM via ICAM1, an adherence molecule that is displayed on the cellular surface under normal conditions. By capturing microbes, ICAM1 displayed by cells of the upper respiratory tract acts as a “GATEWAY” that impedes bacterial invasion of the lower respiratory tract. BRSV infection causes malfunction of ICAM1 in the bovine upper respiratory tract, allowing bacterial invasion of the lower respiratory tract. BRSV infection increased PAFR expression in epithelial cells derived from the lower respiratory tract. We further demonstrated that PM adherence to cells of the lower respiratory tract was enhanced by upregulation of PAFR. Overall, these findings highlight the importance of the synergistic effect of BRSV infection on severe pneumonia in cattle. BCoV infection enhanced PM adherence and triggered immune responses in cells derived from upper and lower respiratory tracts. BCoV infection up-regulated both ICAM1 and PAFR expressions, enhancing the bacteria adherence to the BCoV-infected cells. The interaction between surface receptors and bacteria adherence in upper respiratory tract may prevent severe pneumonia. In contrast, infection of lower respiratory epithelial cells with BCoV increased PM adherence and stimulated an excess immune response, leading to severe pneumonia. Taken together, the present study presents the evidence for distinct mechanism of BRDC-related infections in response to viral infections in respiratory epithelial cells. Understanding this complex interplay will help us to develop new therapeutic strategies for BRDC. Thus, we identify a possible molecular mechanism of fundamental difference of pathogenicity in bovine respiratory virus infections in BRDC.

受賞 【 表示 / 非表示

  • 宮崎日日新聞新聞賞「科学賞」

    2021年10月   宮崎日日新聞社   ブルーベリー葉 の新型コロナ不活化効果

    森下 和広、國武 久登、岡林 環樹、菅本 和寛

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    受賞区分:出版社・新聞社・財団等の賞 

  • 技術賞

    2021年7月   日本オゾン協会   「オゾン水による新型コロナウイルスの不活化」

    稲垣浩子、杉山博信、Putu Eka Sudaryatma, 齊藤暁、岡林環樹、藤元昭一

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    受賞区分:国内学会・会議・シンポジウム等の賞 

科研費(文科省・学振)獲得実績 【 表示 / 非表示

  • 口蹄疫、豚コレラウイルス由来IRES共通因子の探索と制御に向けた基礎的研究

    2020年04月 - 2023年03月

    科学研究費補助金  基盤研究(B)

その他競争的資金獲得実績 【 表示 / 非表示

  • 大学連携による家畜防疫に関する知の集積事業

    2020年04月 - 2022年03月

    民間財団等  全国競馬畜産振興会 

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    資金種別:競争的資金

  • 世界の台所ASEANにおける家畜生産と食品安全に関する新技術導入による畜産革命の推進

    2019年05月

    JICA/JST  地球規模課題対応国際科学技術協力プログラム 

    三澤尚明、水谷哲也 他

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    資金種別:競争的資金