岡林 環樹 (オカバヤシ タマキ)

OKABAYASHI Tamaki

写真a

職名

教授

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  • 酪農学園大学 -  獣医学

 

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  • SFTSウイルスに関する研究

    その他の研究制度  

    研究期間: 2016年04月  -  継続中

     概要を見る

    致死性の高いSFTSウイルスの宮崎県における分布状況を明らかにし、ウイルス学的な解析を行う。これらを活かして診断、治療の基盤情報とする。

  • 牛呼吸器病症候群における複数病原体感染による重症化機序の解明

    科学研究費補助金  

    研究期間: 2015年04月  -  継続中

     概要を見る

    牛呼吸器症候群(BRDC)の実態を明らかにするために、牛呼吸器スワブにおけるBRDC関連病原体遺伝子の検出を試みた。さらに呼吸器上皮細胞を用いたウイルスと細菌の複合感染モデルを作成し、ウイルス感染が及ぼす細菌付着性への影響を調べた。

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  • Co-infection of epithelial cells established from the upper and lower bovine respiratory tract with bovine respiratory syncytial virus and bacteria

    Sudaryatma P., Mekata H., Kubo M., Subangkit M., Goto Y., Okabayashi T.

    Veterinary Microbiology   235   80 - 85   2019年08月

    共著

     概要を見る

    © 2019 Bovine respiratory disease complex is a major disease affecting the global cattle industry. Multiple infections by viruses and bacteria increase disease severity. Previously, we reported that bovine respiratory syncytial virus (BRSV) infection increases adherence of Pasteurella multocida to human respiratory and bovine kidney epithelial cells. To examine the interaction between the virus and bacteria in bovine respiratory cells, we generated respiratory epithelial cell lines from bovine trachea (bTEC), bronchus (bBEC), and lung (bLEC). Although all established cell lines were infected by BRSV and P. multocida susceptibility differed according to site of origin. The cells derived from the lower respiratory tract (bBEC and bLEC) were significantly more susceptible to BRSV than those derived from the upper respiratory tract (bTEC). Pre-infection of bBEC and bLEC with BRSV increased adherence of P. multocida; this was not the case for bTEC. These results indicate that BRSV may reproduce better in the lower respiratory tract and encourage adherence of bacteria. Thus, we identify one possible mechanism underlying severe pneumonia.

    DOI

  • Development of a LAMP assay for rapid and sensitive detection and differentiation of Mycobacterium avium subsp. avium and subsp. hominissuis.

    Yashiki N, Yamazaki Y, Subangkit M, Okabayashi T, Yamazaki W, Goto Y

    Letters in applied microbiology     2019年06月

    共著

    DOI PubMed

  • Genotyping of swine Mycobacterium avium subsp. hominissuis isolates from Kyushu, Japan.

    Subangkit M, Yamamoto T, Ishida M, Nomura A, Yasiki N, Sudaryatma PE, Goto Y, Okabayashi T

    The Journal of veterinary medical science     2019年06月  [査読有り]

    共著

     概要を見る

    <p>The incidence of diseases caused by nontuberculous mycobacteria (NTM) is increasing annually worldwide, including Japan.<i> Mycobacterium avium </i>subsp.<i> hoiminissuis </i>(MAH) is one of the most common NTM species responsible for chronic lung diseases in animals and humans. In the current study, mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing was employed to characterize the genetic diversity of swine MAH isolates from Kyushu, Japan. In total, 309 isolates were obtained from the lymph nodes of 107 pigs not displaying any clinical signs of disease, of which 307 were identified as MAH, comprising 173 strains. Based on eight established MIRU-VNTR loci, the MAH strains represented 50 genotypes constituting three lineages, and 29 had not been described in the Mac French National Institute for Agricultural Research Nouzilly MIRU-VNTR (Mac-INVM) database. MAH was the dominant <i>M. avium</i> complex (MAC) in pigs from Kyushu, and there was high genetic diversity among genotype profiles of MAH from Kyushu. We identified three predominant genotype profiles in the tested area sharing high relatedness with genotype profiles of strains isolated in European countries. MAH was the most common NTM in pigs from Kyushu and exhibited high diversity, with new strain-derived genotypes.</p>

    DOI PubMed CiNii

  • Detection of neutralizing antibody against porcine epidemic diarrhea virus in subclinical infectedfinishing pigs.

    Koike N., Mai TN., Shirai M., Kubo M., Hata K., Marumoto N., Watanabe S., Sasaki Y., Mitoma S., Notsu K., Okabayashi T., Wiratsubaki A. ,Kabali E., Norimine J., Sekiguchi S.

    J. Vet. Med. Sci.     2018年11月  [査読有り]

    共著

  • Mass spectrometry-based identification and whole-genome characterisation of the first pteropine orthoreovirus isolated from monkey faeces in Thailand

    Kosoltanapiwat N., Reamtong O., Okabayashi T., Ampawong S., Rungruengkitkun A., Thiangtrongjit T., Thippornchai N., Leaungwutiwong P., Mahittikorn A., Mori H., Yoohanngoa T., Yamwong P.

    BMC Microbiology   18 ( 1 ) 135   2018年10月  [査読有り]

    共著

     概要を見る

    © 2018 The Author(s). Background: The pteropine orthoreovirus (PRV) was isolated from monkey (Macaca fascicularis) faecal samples collected from human-inhabited areas in Lopburi Province, Thailand. These samples were initially obtained to survey for the presence of hepatitis E virus (HEV). Results: Two virus isolates were retrieved by virus culture of 55 monkey faecal samples. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was successfully used to identify the viruses as the segmented dsRNA orthoreovirus. Phylogenetic analysis of the Lopburi orthoreovirus whole-genomes revealed relationships with the well-characterised PRVs Pulau (segment L1), Cangyuan (segments L2, M3 and S3), Melaka (segments L3 and M2), Kampar (segments M1 and S2) and Sikamat (segments S1 and S4) of Southeast Asia and China with nucleotide sequence identities of 93.5-98.9%. RT-PCR showed that PRV was detected in 10.9% (6/55) and HEV was detected in 25.5% (14/55) of the monkey faecal samples. Conclusions: PRV was isolated from monkey faeces for the first time in Thailand via viral culture and LC-MS/MS. The genetic diversity of the virus genome segments suggested a re-assortment within the PRV species group. The overall findings emphasise that monkey faeces can be sources of zoonotic viruses, including PRV and HEV, and suggest the need for active virus surveillance in areas of human and monkey co-habitation to prevent and control emerging zoonotic diseases in the future.

    DOI PubMed

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著書 【 表示 / 非表示

  • 獣医微生物学 第4版

    関崎 勉 他 (担当: 共著 )

    文永堂  2018年07月

総説・解説記事 【 表示 / 非表示

  • Diversity of suppression mechanisms of JAK/STAT pathway by virus infection

    Okabayashi Tamaki

    Tanpakushitsu Kakusan Koso.     2004年

    総説・解説(大学・研究所紀要)   単著

その他競争的資金獲得実績 【 表示 / 非表示

  • 世界の台所ASEANにおける家畜生産と食品安全に関する新技術導入による畜産革命の推進

    提供機関:  JICA/JST  地球規模課題対応国際科学技術協力プログラム

    研究期間: 2019年05月  -  継続中  代表者:  三澤尚明

共同研究希望テーマ 【 表示 / 非表示

  • 牛呼吸器症候群におけるウイルスの役割

  • 豚ウイルス下痢症をはじめとする豚ウイルスの変異が及ぼす抗原性への影響

  • 宮崎県における重症熱性血小板減少症候群の分布状況とその感染サイクルの解明

  • 抗酸菌症に関する研究

  • 節足動物媒介性感染症に関する研究