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The expression of the formin Fhod3 in mouse tongue striated muscle 査読あり
Nakagawa H., Kage Y., Miura A., Sulistomo H.W., Matsuyama S., Yamashita Y., Takeya R.
Cell Structure and Function 49 ( 2 ) 111 - 122 2024年11月
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Cell Structure and Function
The sarcomere is the contractile unit of striated muscle and is composed of actin and myosin filaments. There is increasing evidence to support that actin assembly mediated by Fhod3, a member of the formin family of proteins, is critical for sarcomere formation and maintenance in cardiac muscle. Fhod3, which is abundantly expressed in the heart, localizes to the center of sarcomeres and contributes to the regulation of the cardiac function, as evidenced by the fact that mutations in Fhod3 cause cardiomyopathy. However, the role of Fhod3 in skeletal muscle, another type of striated muscle, is unclear. We herein show that Fhod3 is expressed in the tongue at both mRNA and protein levels, although in smaller amounts than in the heart. To determine the physiological role of Fhod3 expressed in the tongue, we generated embryos lacking Fhod3 in the tongue. The tongue tissue of the Fhod3-depleted embryos did not show any significant structural defects, suggesting that Fhod3 is dispensable for normal development of the mouse tongue. Unexpectedly, the immunostaining analysis revealed the absence of specific sarcomeric signals for Fhod3 in the wild-type tongue when compared to the Fhod3-depleted tongue as a negative control, despite the use of antibodies that had previously been validated by immunostaining of heart tissues. Taken together, although Fhod3 protein is expressed at a significant level in the tongue, Fhod3 in the tongue does not appear to exhibit the same sarcomeric pattern as observed in the heart, suggesting a different role for Fhod3 in the tongue muscles.
DOI： 10.1247/csf.24044
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Interaction between cardiac myosin-binding protein C and formin Fhod3 査読あり
Matsuyama S., Kage Y., Fujimoto N., Ushijima T., Tsuruda T., Kitamura K., Shiose A., Asada Y., Sumimoto H., Takeya R.
Proceedings of the National Academy of Sciences of the United States of America 115 ( 19 ) E4386 - E4395 2018年5月
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Proceedings of the National Academy of Sciences of the United States of America
© 2018 National Academy of Sciences. All rights reserved. Mutations in cardiac myosin-binding protein C (cMyBP-C) are a major cause of familial hypertrophic cardiomyopathy. Although cMyBP-C has been considered to regulate the cardiac function via cross-bridge arrangement at the C-zone of the myosin-containing A-band, the mechanism by which cMyBP-C functions remains unclear. We identified formin Fhod3, an actin organizer essential for the formation and maintenance of cardiac sarcomeres, as a cMyBP-C–binding protein. The cardiac-specific N-terminal Ig-like domain of cMyBP-C directly interacts with the cardiac-specific N-terminal region of Fhod3. The interaction seems to direct the localization of Fhod3 to the C-zone, since a noncardiac Fhod3 variant lacking the cMyBP-C–binding region failed to localize to the C-zone. Conversely, the cardiac variant of Fhod3 failed to localize to the C-zone in the cMyBP-C–null mice, which display a phenotype of hypertrophic cardiomyopathy. The cardiomyopathic phenotype of cMyBP-C–null mice was further exacerbated by Fhod3 overexpression with a defect of sarcomere integrity, whereas that was partially ameliorated by a reduction in the Fhod3 protein levels, suggesting that Fhod3 has a deleterious effect on cardiac function under cMyBP-C–null conditions where Fhod3 is aberrantly mislocalized. Together, these findings suggest the possibility that Fhod3 contributes to the pathogenesis of cMyBP-C–related cardiomyopathy and that Fhod3 is critically involved in cMyBP-C–mediated regulation of cardiac function via direct interaction.
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Role of Src homology 3 domains in assembly and activation of the phagocyte NADPH oxidase 査読あり
Sumimoto H., Kage Y., Nunoi H., Sasaki H., Nose T., Fukumaki Y., Ohno M., Minakami S., Takeshige K.
Proceedings of the National Academy of Sciences of the United States of America 91 ( 12 ) 5345 - 5349 1994年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Proceedings of the National Academy of Sciences of the United States of America
The phagocyte NADPH oxidase, dormant in resting cells, is activated during phagocytosis to produce superoxide, a precursor of microbicidal oxidants. The activated oxidase is a complex of membrane-integrated cytochrome b 558 , composed of 91-kDa (gp91(phox)) and 22-kDa (p22(phox)) subunits, and two cytosolic factors (p47(phox) and p67(phox)), each containing two Src homology 3 (SH3) domains. Here we show that the region of the tandem SH3 domains of p47(phox) (p47-SH3) expressed as a glutathione S-transferase fusion protein inhibits the superoxide production in a cell-free system, indicating involvement of the domains in the activation. Furthermore, we find that arachidonic acid and sodium dodecyl sulfate, activators of the oxidase in vitro, cause exposure of p47-SH3, which has probably been masked by the C- terminal region of this protein in a resting state. The unmasking of p47-SH3 appears to play a crucial role in the assembly of the oxidase components, because p47-SH3 binds to both p22(phox) and p67(phox) but fails to interact with a mutant p22(phox) carrying a Pro-156 → Gln substitution in a proline- rich region, which has been found in a patient with chronic granulomatous disease. Based on the observations, we propose a signal-transducing mechanism whereby normally inaccessible SH3 domains become exposed upon activation to interact with their target proteins.
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Maruta T., Hidaka K., Kouroki S., Koshida T., Kurogi M., Kage Y., Mizuno S., Shirasaka T., Yanagita T., Takahashi S., Takeya R., Tsuneyoshi I.
PLoS ONE 17 ( 10 October ) e0275751 2022年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：PLoS ONE
In small and large spinal dorsal root ganglion neurons, subtypes of voltage-gated sodium channels, such as NaV1.7, NaV1.8, and NaV1.9 are expressed with characteristically localized and may play different roles in pain transmission and intractable pain development. Selective stimulation of each specific subtype in vivo may elucidate its role of each subtype in pain. So far, this has been difficult with current technology. However, Optogenetics, a recently developed technique, has enabled selective activation or inhibition of specific neural circulation in vivo. Moreover, optogenetics had even been used to selectively excite NaV1.8-expressing dorsal root ganglion neurons to induce nocifensive behavior. In recent years, genetic modification technologies such as CRISPR/Cas9 have advanced, and various knock-in mice can be easily generated using such technology. We aimed to investigate the effects of selective optogenetic activation of NaV1.7-expressing afferents on mouse behavior. We used CRISPR/Cas9-mediated homologous recombination to generate bicistronic NaV1.7–iCre knock-in mice, which express iCre recombinase under the endogenous NaV1.7 gene promoter without disrupting NaV1.7. The Cre-driver mice were crossed with channelrhodopsin-2 (ChR2) Cre-reporter Ai32 mice to obtain NaV1.7iCre/+;Ai32/+, NaV1.7iCre/iCre;Ai32/+, NaV1.7iCre/+;Ai32/Ai32, and NaV1.7iCre/iCre;Ai32/Ai32 mice. Compared with wild–type mice behavior, no differences were observed in the behaviors associated with mechanical and thermal stimuli exhibited by mice of the aforementioned genotypes, indicating that the endogenous NaV1.7 gene was not affected by the targeted insertion of iCre. Blue light irradiation to the hind paw induced paw withdrawal by mice of all genotypes in a light power-dependent manner. The threshold and incidence of paw withdrawal and aversive behavior in a blue-lit room were dependent on ChR2 expression level; the strongest response was observed in NaV1.7iCre/iCre;Ai32/Ai32 mice. Thus, we developed a non-invasive pain model in which peripheral nociceptors were optically activated in free-moving transgenic NaV1.7–ChR2 mice.
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Kouroki S., Maruta T., Hidaka K., Koshida T., Kurogi M., Kage Y., Miura A., Nakagawa H., Yanagita T., Takeya R., Tsuneyoshi I.
Plos One 20 ( 5 May ) e0323628 2025年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Plos One
Neuropathic pain has a significant social impact, with high morbidity and reduced productivity, the underlying mechanisms of neuropathic pain remain poorly understood, and effective therapeutic strategies remain elusive. The development of animal models of neuropathic pain that stimulate only the nociceptors and not the other sensory receptors or motor nerves is desirable for elucidating the complex pathogenesis of neuropathic pain. We have previously reported the generation of Na<inf>V</inf>1.7−channelrhodopsin-2 (ChR2), Na<inf>V</inf>1.8−ChR2, and Na<inf>V</inf>1.9−ChR2 mice. Optogenetics was employed in these light-responsive pain mice for generating nociceptive pain by specifically exciting the spinal dorsal root ganglion neurons, in which the respective Na<sup>+</sup> channels are expressed through exposure to blue light. This study aimed to compare the neuropathic pain produced by the prolonged exposure of light-responsive pain mice to blue light. A reversible neuropathic pain state was established persisting for a minimum of 24 hours when each light-responsive pain mouse was irradiated with light of an intensity that consistently elicited pain. Furthermore, the mice also showed pain sensitivity to light irradiation and mechanical stimulation. The expression of c-Fos, a marker for neuronal activity following noxious stimulation, was increased in the dorsal horn of the spinal cord on the light irradiated side. DS-1971a, a selective Na<inf>V</inf>1.7 inhibitor, was effective in attenuating neuropathic pain in all light-responsive pain mice. In conclusion, optogenetics helps elucidate the specific functions of sodium channel subtypes in pain signaling, thereby advancing our understanding and paving the way for the development of further effective treatments for pain disorders in the future.
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マウス舌組織におけるフォルミン蛋白質Fhod3の発現様式
中川光，○鹿毛陽子，三浦綾子，武谷立
令和6年度日本生化学会九州支部例会 2024年6月22日
開催年月日： 2024年6月22日 - 2024年6月23日
記述言語：日本語 会議種別：ポスター発表
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マウス大脳皮質におけるフォルミン蛋白質の発現パターン
鹿毛陽子, Hikmawan Wahyu Sulistomo, 武谷立
第93回日本生化学会 (京都（WEB開催）) 日本生化学会
開催年月日： 2020年9月14日 - 2020年9月16日
記述言語：日本語 会議種別：ポスター発表
開催地：京都（WEB開催）
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アクチン重合制御因子フォルミン蛋白質のマウス大脳皮質における発現
鹿毛陽子, Hikmawan Wahyu Sulistomo, 武谷立
第73回日本薬理学会西南部会 (熊本県（WEB開催）) 日本薬理学会西南部会
開催年月日： 2020年11月21日
記述言語：日本語 会議種別：ポスター発表
開催地：熊本県（WEB開催）
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サルコメア恒常性の破綻がもたらす心筋細胞の形態変化と細胞死.
鹿毛陽子，阪口修平，武谷 立
第96回日本生化学会大会 2023年11月1日
開催年月日： 2023年10月31日 - 2023年11月2日
記述言語：日本語 会議種別：ポスター発表
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舌筋におけるフォルミン蛋白質Fhod3の発現と分布.
中川光，鹿毛 陽子，武谷立
第96回日本生化学会大会 2023年11月1日
開催年月日： 2023年10月31日 - 2023年11月2日
記述言語：日本語 会議種別：ポスター発表
科研費（文科省・学振・厚労省）獲得実績 【 表示 ／ 非表示 】
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骨格筋サルコメアの恒常性維持機構ならびにその破綻として捉えるサルコペニア
研究課題/領域番号：22K11754 2022年04月 - 2026年03月
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
担当区分：研究代表者
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サルコメアは回転トルクを生み出すか？
研究課題/領域番号：22K19407 2022年04月 - 2024年03月
独立行政法人日本学術振興会 科学研究費補助金 挑戦的研究(萌芽)
武谷 立、
担当区分：研究分担者
その他競争的資金獲得実績 【 表示 ／ 非表示 】
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骨格筋のターンオーバー機構からサルコペニアに挑む
2022年04月 - 2023年03月
宮崎大学 女性研究者の特色ある研究
担当区分：研究代表者
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Athenaリサーチアシスタント制度
2022年08月 - 2023年03月
宮崎大学 令和4年度「出産・育児・介護に関わる教職員に対する研究補助者雇用」
担当区分：研究代表者
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骨格筋収縮装置の恒常性維持とその破綻の分子機構
2019年04月 - 2020年03月
宮崎大学 戦略重点経費-女性研究者の特色ある研究
資金種別：競争的資金