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Upregulation of MAP kinase HOG1 gene of white-rot fungus Phlebia sp. MG-60 inhibits the ethanol fermentation and mycelial growth. 査読あり
Motoda T, Chen FC, Tsuyama T, Tokumoto Y, Kijidani Y, Kamei I
Bioscience, biotechnology, and biochemistry 87 ( 2 ) 217 - 227 2023年1月
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Culture Conditions for Mycelial Growth and Anti-Cancer Properties of Termitomyces 査読あり
Tharavecharak S., D’Alessandro-Gabazza C.N., Toda M., Yasuma T., Tsuyama T., Kamei I., Gabazza E.C.
Mycobiology 2023年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Mycobiology
Termitomyces sp. that grow in symbiosis with fungus-farming Termites have medicinal properties. However, they are rare in nature, and their artificial culture is challenging. The expression of AXL receptor tyrosine kinase and immune checkpoint molecules favor the growth of cancer cells. The study evaluated the optimal conditions for the artificial culture of Termitomyces and their inhibitory activity on AXL and immune checkpoint molecules in lung adenocarcinoma and melanoma cell lines. The culture of 45 strains of Termitomyces was compared. Five strains with marked growth rates were selected. Four of the selected strains form a single cluster by sequence analysis. The mycelium of 4 selected strains produces more fungal mass in potato dextrose broth than in a mixed media. The bark was the most appropriate solid substrate for Termitomyces mycelia culture. The mycelium of all five selected strains showed a higher growth rate under normal CO2 conditions. The culture broth, methanol, and ethyl acetate of one selected strain (T-120) inhibited the mRNA relative expression of AXL receptor tyrosine kinase and immune checkpoint molecules in cancer cell lines. Overall, these results suggest the potential usefulness of Termitomyces extracts as a co-adjuvant therapy in malignant diseases.
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Kijidani Y., Tsuyama T., Oda H., Hirata R., Ito S.
Journal of Wood Science 68 ( 1 ) 2022年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Wood Science
The current stumpage price in harvesting of a 50-year-old sugi (Japanese cedar, Cryptomeria japonica) plantation is not enough to motivate forest owners to reforest. Therefore, it is vital to reduce the cost for reforestation after logging as well as for logging and wood transportation of the preceding stands. Low initial tree density has been emphasized as one of the important basic measures to reduce directly the reforestation costs. In this study, for better understanding of the mechanical properties and dimensional stability of lumbers from sugi trees grown in low initial tree density, we examined the effects of initial tree density on wood density and microfibril angle of the S2 layer in the secondary wall of tracheids in rings from pith to bark in a sugi cultivar (Tosaaka) grown in a Nelder plot (initial density zoning symbols; D (4823 trees/ha), E (3349 trees/ha), G (1615 trees/ha), H (1122 trees/ha) and J (541 trees/ha)). Ring width and latewood percentage in J tree (541 trees/ha) were significantly larger and smaller than those of other initial tree density zoning symbol trees, respectively. Average wood density and earlywood wood density in J tree (541 trees/ha) were significantly smaller than those of other initial tree density zoning symbol trees (Tukey’s honestly significant difference test (Tukey HSD), p < 0.05). However, effects of initial tree density on the microfibril angles (earlywood and latewood) in rings from pith to bark and the transition patterns of microfibril angle from earlywood to latewood in an outer ring (ring number 22) were not significant or very small. Heartwood width in H trees (1122 trees/ha) and J trees (541 trees/ha) were significantly larger than those in other initial tree density zoning symbol trees (Tukey HSD, p < 0.05). There were no significant differences of ring width, average wood density, earlywood density and microfibril angle between H trees (1122 trees/ha) and E trees (3349 trees/ha) in many rings from pith to bark (Tukey HSD, p > 0.05). Based on these results of Tosaaka, a sugi cultivar grown in a Nelder plot, it was assumed that mechanical properties and dimensional stability of lumbers from sugi trees grown in low initial tree density (1000 trees/ha) might be quite similar to those from sugi trees grown in the regular initial tree density (3000 trees/ha), although lumbers from J trees (541 trees/ha) might be different from those of sugi trees grown in the regular initial tree density (3000 trees/ha). By taking the effects of genetic factor (variation of sugi cultivars) and the interaction between genetic and environmental factor (initial tree density) into consideration, the effects of low initial tree density (1000 trees/ha) on mechanical properties and dimensional stability of lumbers from sugi plantations in Japan might be smaller than the effects recognized in Tosaaka in this study. In this study, we did not examine the effects of initial tree density on the knots and the cross grain of lumbers. Low initial tree density might increase the negative effects of knots and cross grain on mechanical properties of lumbers. However, recent wood processing technology could minimize these negative effects.
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Deposition patterns of feruloylarabinoxylan during cell wall formation in moso bamboo 査読あり
Munekata N., Tsuyama T., Kamei I., Kijidani Y., Takabe K.
Planta 256 ( 3 ) 59 2022年9月
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Planta
Main conclusion: The feruloylarabinoxylan deposition was initiated at the formation of the secondary cell wall, especially S2 layer in moso bamboo, which may affect crosslinking between cell wall components and plant growth. Abstract: Hemicelluloses, major components of plant cell walls that are hydrogen bonded to cellulose and covalently bound to lignin, are crucial determinants of cell wall properties. Especially in commelinid monocotyledons, arabinoxylan is often esterified with ferulic acid, which is essential to crosslinking with cell wall components. However, the deposition patterns and localization of ferulic acid during cell wall formation remain unclear. In this study, developing moso bamboo (Phyllostachys pubescens) culms were used to elucidate deposition patterns of hemicelluloses including feruloylarabinoxylan. Ferulic acid content peaked with cessation of elongation growth, and thereafter decreased and remained stable as culm development proceeded. During primary cell wall (PCW) formation, xyloglucan and (1,3;1,4)-β-glucan signals were detected in all tissues. Along with culm development, arabinoxylan and feruloylarabinoxylan signals were sequentially observed in the protoxylem, vascular fibers and metaxylem, and parenchyma. Feruloylarabinoxylan signals were observed slightly later than arabinoxylan signals. Arabinoxylan signals were observed throughout the compound middle lamella and secondary cell wall (SCW), whereas the feruloylarabinoxylan signal was localized to the S2 layer of the SCW. These results indicate that the biosynthesis of hemicelluloses is regulated in accordance with cell wall layers. Feruloylarabinoxylan deposition may be initiated at the formation of SCW, especially S2 layer formation. Ferulic acid-mediated linkages of arabinoxylan-arabinoxylan and arabinoxylan-lignin would arise during SCW formation with the cessation of elongation growth.
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Kijidani Y., Tsuyama T., Takata K.
Journal of Wood Science 67 ( 1 ) 2021年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Wood Science
We previously reported the species-specific annual ring formation characteristics of three conifers (slash pine (Pinus elliottii), hinoki (Japanese cypress, Chamaecyparis obtusa) and sugi (Japanese cedar, Cryptomeria japonica)) grown in the same stand over 2 years. We found that the species-specific annual ring formation characteristics affected the inherent difference in wood density among these conifers (slash pine > hinoki > sugi). Plant hormones in cambial-region tissues were believed to affect annual ring formation. However, seasonal variation of the amounts of plant hormones in cambial-region tissues had only been examined in a few tree species. In this study, as the first step to elucidating the role of plant hormones in annual ring formation in conifers, we report the seasonal variations of the auxin (indole acetic acid, IAA) and gibberellin A4 (GA4) levels in cambial-region tissues and their effects on annual ring formation in three conifers (slash pine, hinoki, and sugi) with inherently different wood densities. Sugi (small wood density) had significantly higher levels of IAA and formed more tracheids in the early season than in the late season, although slash pine (large wood density) had higher levels of IAA and formed significantly more tracheids in the late season than in the early season. Hinoki (intermediate wood density) had constant IAA levels and formed a constant number of tracheids throughout the season. There were significant positive correlations between the levels of IAA in cambial-region tissues and the number of tracheids formed during late season in the two conifer species. A close relationship was observed between the seasonal ratio of the IAA levels (late/early season) and wood density. No consistent trend in the change in the level of IAA during the transition from earlywood to latewood formation was recognized among the three conifers. The IAA levels in slash pines were significantly higher than those in sugi and hinoki. The GA4 levels had no significant effect on number of tracheids formed in the three conifers. These results suggest that the species-specific seasonal variation patterns of the IAA levels might lead to the inherent differences in wood density among these three conifers through species-specific characteristics in the formation of annual rings.
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Maeda H., Tsuyama T., Takabe K., Kamitakahara H., Takano T.
Journal of Wood Science 66 ( 1 ) 2020年12月
記述言語：日本語 掲載種別：記事・総説・解説・論説等（大学・研究所紀要） 出版者・発行元：Journal of Wood Science
© 2020, The Author(s). In the original publication of the article, the letter “l” in the section headings was published incorrectly as “l”. It should appear as “l” throughout the article [1]. In the “Results and discussion” section, the second sentence in the first paragraph was published incorrectly as “Compound 1D was also prepared……..compound 6L”. It should read as “Compound 1D was also prepared…….. compound 1L”. The caption of Fig. 3 was published incorrectly as “1H NMR spectra of compounds 1L (a) and 1D (b); 13C NMR spectra of compounds 1L (a′) and 1D (b′)”. The corrected Fig. 3 caption should read as “1H NMR spectra of compounds 1L (a) and 1D (b); 13C NMR spectra of compounds 1L (a’) and 1D (b’)”.
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樹木分化中木部におけるリグニン前駆物質の輸送
津山 濯
アグリバイオ 2019年6月
記述言語：日本語 掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）
講演・口頭発表等 【 表示 ／ 非表示 】
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白色腐朽菌Phlebia sp. MG-60 株におけるMAPキナーゼHOG1の発現増強はエタノール発酵と菌糸成長を抑制する
元田多一、陳 富嘉、津山 濯、徳本雄史、亀井一郎、雉子谷佳男
第73回日本木材学会大会
開催年月日： 2023年3月14日 - 2023年3月16日
会議種別：口頭発表（一般）
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モウソウチクにおけるフェルラ酸転移酵素候補遺伝子PeBAHD1の発現制御に関与する転写因子の探索
宗像典哲、津山 濯、雉子谷佳男、高田直樹、坂本真吾、光田展隆
第73回日本木材学会大会
開催年月日： 2023年3月14日 - 2023年3月16日
会議種別：口頭発表（一般）
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マルチラベリングU-NETモデルによるモウソウチク横断面像の組織識別
平井成実、浜井堅成、陳 碩也、津山 濯、雉子谷佳男、杉山淳司
第73回日本木材学会大会
開催年月日： 2023年3月14日 - 2023年3月16日
会議種別：ポスター発表
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Developmental process and the occurrence of cell death in the ant defense organ revealed by gene expression patterns 国際会議
Chongmeng Xu, Eri Yamasaki, Taku Tsuyama, Yuji Tokumoto, Yasuhiro Sato, Kentaro K. Shimizu
Biology 2023
開催年月日： 2023年2月16日 - 2023年2月17日
会議種別：口頭発表（一般）
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Comparative transcriptome analysis reveals domatia development in ant symbiotic plants 国際会議
Chongmeng Xu, Eri Yamasaki, Taku Tsuyama, Yuji Tokumoto, Yasuhiro Sato, Kentaro K. Shimizu
BES annual meeting 2022
開催年月日： 2022年12月18日 - 2022年12月21日
会議種別：ポスター発表
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第21回黎明研究者賞 口頭発表部門
2019年9月 日本木材学会九州支部
島田 菜津美、津山 濯、松下 泰幸、福島 和彦、矢崎 一史、高部 圭司、亀井 一郎
受賞区分：国内学会・会議・シンポジウム等の賞 受賞国：日本国
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第21回黎明研究者賞 ポスター発表部門
2019年9月 日本木材学会九州支部
宗像典哲、津山 濯、雉子谷佳男、高部圭司、亀井一郎
受賞区分：国内外の国際的学術賞 受賞国：日本国
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第28回 日本木材学会奨励賞
2017年3月 日本木材学会
津山 濯
受賞区分：国内学会・会議・シンポジウム等の賞 受賞国：日本国
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Best Poster Presentation
2009年8月 Pacific Regional Wood Anatomy Conference
Taku Tsuyama, Keiji Takabe
受賞区分：国際学会・会議・シンポジウム等の賞 受賞国：マレーシア
科研費（文科省・学振・厚労省）獲得実績 【 表示 ／ 非表示 】
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多様なリグニン前駆物質の木化組織における輸送メカニズム
研究課題/領域番号：23K05340 2023年04月 - 2026年03月
科学研究費補助金 基盤経費（C)
担当区分：研究代表者
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植物細胞壁S2層形成の制御メカニズム
研究課題/領域番号：20H03049 2020年04月 - 2023年03月
科学研究費補助金 基盤研究(B)
担当区分：研究代表者
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ポプラ分化中木部におけるコニフェリン輸送体の同定
2017年04月 - 2020年03月
科学研究費補助金 若手研究(B)
担当区分：研究代表者
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分化中木部におけるリグニン前駆物質輸送メカニズムの解明
2011年04月 - 2013年03月
科学研究費補助金 特別研究員奨励費