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医学部 医学科 感染症学講座寄生虫学分野 |
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論文 【 表示 ／ 非表示 】
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Tanaka R., Kanzaki N., Giblin-Davis R.M.
Nematology 27 ( 1 ) 37 - 48 2025年
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Nematology
Schistonchus pumilae was first reported from the syconi of a creeper fig, Ficus pumila, from Miyazaki, Japan, and the species was described based on propagative (plant-parasitic) females and males. Thus, the premature female (entomophilic form) of the species was not described. In the present study, male syconi of F. pumila were collected from the type locality of the nematode (Miyazaki, Japan), and the premature females of S. pumilae were collected from inside the syconi and also from the emerging pollinator fig wasps (Wiebesia pumilae). Although only 26 female wasps were recovered for dissections, seven of them harboured 1-7 nematodes each. Nematodes associated with the wasp were all premature females, and their typological and morphometric characters were similar with those isolated from inside the syconi. In addition, the premature females were slightly smaller than propagative females and had a less developed gonad, suggesting that the nematode is a phoretic associate, not a parasite. Regardless of its phylogenetic status, the carrier insect usage of S. pumilae is somewhat similar to two Ficophagus species, and clearly different from S. caprifici, which propagates in the host wasp haemocoel as a parasite. Additionally, the molecular profiles of S. pumilae (SSU and D1-D3 LSU of ribosomal RNA genes) were re-sequenced using a cloning technique, and the information was updated for phylogenetic analysis in the future.
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Kawahara K, Inada T, Tanaka R, Dayi M, Makino T, Maruyama S, Kikuchi T, Sugimoto A, Kawata M
Genome biology and evolution 15 ( 4 ) 2023年4月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Genome biology and evolution
Why the recently discovered nematode Caenorhabditis inopinata differs so greatly from its sibling species Caenorhabditis elegans remains unknown. A previous study showed that C. inopinata has more transposable elements (TEs), sequences that replicate and move autonomously throughout the genome, potentially altering the expression of neighboring genes. In this study, we focused on how the body size of this species has evolved and whether TEs could affect the expression of genes related to species-specific traits such as body size. First, we compared gene expression levels between C. inopinata and C. elegans in the L4 larval and young adult stages-when growth rates differ most prominently between these species-to identify candidate genes contributing to their differences. The results showed that the expression levels of collagen genes were consistently higher in C. inopinata than in C. elegans and that some genes related to cell size were differentially expressed between the species. Then, we examined whether genes with TE insertions are differentially expressed between species. Indeed, the genes featuring C. inopinata-specific TE insertions had higher expression levels in C. inopinata than in C. elegans. These upregulated genes included those related to body size, suggesting that these genes could be candidates for artificial TE insertion to examine the role of TEs in the body size evolution of C. inopinata.
DOI： 10.1093/gbe/evad063
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Ko P.P., Haraguchi M., Hara T., Hieu D.D., Ito A., Tanaka R., Tanaka M., Suzumura T., Ueda M., Yoshida A., Maruyama H., Nagayasu E.
Parasitology International 92 102663 2023年2月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Parasitology International
Strongyloides is a genus of parasitic nematodes of vertebrates comprising approximately 50 documented species, each with various host ranges. Among these, three species (S. stercoralis, S. fuelleborni, and S. cebus) are known to infect primate hosts. S. fuelleborni typically infects non-human primates in the Old World. To complement the existing information on the global genetic structure of this species, we conducted a genotyping study of S. fuelleborni samples collected from rhesus macaques in Myanmar, Japanese macaques in Japan, and some zoo-kept primates. This study identified a novel haplotype group in isolates from the Myanmar rhesus macaques. Subsequently, we obtained the complete or nearly complete mitochondrial genome sequences of S. fuelleborni, S. cebus (Strongyloides of New World monkeys), and S. vituli (Strongyloides of cattle). Phylogenetic analysis based on concatenated mitochondrial protein sequences of various Strongyloides species indicated a close relationship between S. fuelleborni, S. vituli and S. papillosus (Strongyloides in sheep and cattle). S. cebus is quite distantly related to both S. fuelleborni and S. stercoralis, which led to the hypothesis that the three primate Strongyloides species evolved independently as parasites of primates.
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Kanzaki N., Tanaka R., Giblin-Davis R.M.
Nematology 25 ( 5 ) 509 - 529 2023年
掲載種別：研究論文（学術雑誌） 出版者・発行元：Nematology
A Schistonchus species was isolated from the syconia of the creeping fig, Ficus pumila, collected from Miyazaki, Japan. The nematode was considered an undescribed species based on its typological characters, molecular profiles (near full length of small subunit and D3 expansion segment of large subunit of RNA) and phylogenetic status inferred from these two loci. The new species is characterised by the presence of flagellate sperm, spicule possessing small condylus, triangular rostrum with bluntly pointed tip and clear dorsal and ventral limbs, structure and arrangement of male caudal papillae, i.e., presence of papilliform P3 and P3a and small and gland opening-like P4 (glandpapillae), relatively short post-uterine sac and female tail forming elongate conoid. It is phylogenetically close to S. hirtus. However, the new species is distinguished from other nominal Schistonchus species by its female tail shape forming elongate conoid, the structure and arrangement of male caudal papillae and several other typological characters. In addition, the host fig of the nematode, F. pumila, is a creeper fig species belonging to subgenus Synoecia (section Rhizocladus; subsection Plagiostigma), from which no fig-Associated nematode has been reported so far, i.e., the present study is the first report of Schistonchus (and other syconia-inhabiting nematodes) from the Ficus subgenus Synoecia. Keywords
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Sun S., Kanzaki N., Dayi M., Maeda Y., Yoshida A., Tanaka R., Kikuchi T.
BMC Genomics 23 ( 1 ) 765 2022年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：BMC Genomics
Background: The first metazoan genome sequenced, that of Caenorhabditis elegans, has motivated animal genome evolution studies. To date > 50 species from the genus Caenorhabditis have been sequenced, allowing research on genome variation. Results: In the present study, we describe a new gonochoristic species, Caenorhabditis niphades n. sp., previously referred as C. sp. 36, isolated from adult weevils (Niphades variegatus), with whom they appear to be tightly associated during its life cycle. Along with a species description, we sequenced the genome of C. niphades n. sp. and produced a chromosome-level assembly. A genome comparison highlighted that C. niphades n. sp. has the smallest genome (59 Mbp) so far sequenced in the Elegans supergroup, despite being closely related to a species with an exceptionally large genome, C. japonica. Conclusions: The compact genome of C. niphades n. sp. can serve as a key resource for comparative evolutionary studies of genome and gene number expansions in Caenorhabditis species.
書籍等出版物 【 表示 ／ 非表示 】
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Nematodes Exploiting P. japonensis
Toyoshi Yoshiga, Ryusei Tanaka, Etsuko Okamura（ 担当： 分担執筆）
Springer Nature 2022年10月
著書種別：学術書
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生き物と音の辞典
高梨 琢磨 他（ 担当： 共著 , 範囲: 線虫の振動受容）
朝倉書店 2019年9月
総ページ数：441 担当ページ：336-337 記述言語：日本語 著書種別：学術書
音と音に類する刺激に対する様々な生物の反応の知見をまとめた著書。線虫の振動受容について担当。
講演・口頭発表等 【 表示 ／ 非表示 】
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多数の虫体が検出されたマンソン孤虫症
田中龍聖、田中美緒、山﨑佳那子、長安英治、久保宜明、丸山治彦
第93回 日本寄生虫学会大会 2024年3月9日
開催年月日： 2024年3月9日 - 2024年3月10日
記述言語：日本語 会議種別：口頭発表（一般）
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ネズミ糞線虫Strongyloides rattiの自由生活世代と細菌の関係
田中龍聖・石田まり・太田有咲・地下勇矢・丸山治彦
第92回日本寄生虫学会大会 2022年5月
開催年月日： 2022年5月30日 - 2022年5月31日
会議種別：ポスター発表
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Relationship of Caenorhabditis inopinata with the wasp and bacteria in the fig ecosystem 国際会議
Ryusei Tanaka, Tanzila Afrin and Taisei Kikuchi
Seventh International Congress of Nematology 2022年5月
開催年月日： 2022年5月1日 - 2022年5月6日
会議種別：ポスター発表
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オオバイヌビワ果実内部の線虫の動態
田中龍聖,Afrin tanzila,菊地泰生
2018 日本線虫学会大会 (熊本) 日本線虫学会
開催年月日： 2018年9月5日 - 2018年9月6日
記述言語：日本語 会議種別：口頭発表（一般）
開催地：熊本
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イチジク果実内部に生息する線虫Caenorhabdits sp. 34 の餌としての細菌
田中龍聖・神崎菜摘・菊地泰生
日本線虫学会
開催年月日： 2016年9月14日 - 2016年9月16日
記述言語：日本語 会議種別：口頭発表（一般）
科研費（文科省・学振・厚労省）獲得実績 【 表示 ／ 非表示 】
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線虫における種分化機構の複合的理解
研究課題/領域番号：24K09595 2024年04月 - 2027年03月
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
担当区分：研究代表者
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イチジク果実内の生物群の共進化
研究課題/領域番号：16K18608 2016年04月 - 2019年03月
科学研究費補助金 若手研究(B)
担当区分：研究代表者