UTO Takuya



Engineering educational research section Department of Applied Chemistry Program


Associate Professor

Laboratory Address


External Link

Degree 【 display / non-display

  • 博士(工学) ( 2014.9   宮崎大学 )

  • 修士(工学) ( 2012.3   宮崎大学 )

  • 学士(教育学) ( 2010.3   宮崎大学 )

Research Interests 【 display / non-display

  • 計算化学

Research Areas 【 display / non-display

  • Nanotechnology/Materials / Fundamental physical chemistry  / 基礎物理化学

  • Nanotechnology/Materials / Polymer chemistry  / 生体高分子化学

  • Informatics / Life, health and medical informatics  / 計算化学

Education 【 display / non-display

  • 宮崎大学大学院   農学工学総合研究科   生物機能応用科学専攻

    2012.4 - 2014.9

  • 宮崎大学大学院   工学研究科   物質環境化学専攻

    2010.4 - 2012.3

  • University of Miyazaki

    2006.4 - 2010.3

Campus Career 【 display / non-display

  • University of Miyazaki   Engineering educational research section   Department of Applied Chemistry Program   Associate Professor

    2021.11 - Now

  • University of Miyazaki   Organization for Promotion of Career Management   Institute for Tenure Track Promotion   Assistant Professor

    2020.01 - 2021.10

  • University of Miyazaki   Tenure Track System Organization   Assistant Professor

    2018.11 - 2019.12

External Career 【 display / non-display

  • 日本学術振興会特別研究員(PD)   鹿児島大学大学院理工学研究科   日本学術振興会特別研究員

    2016.4 - 2018.10

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  • 日本学術振興会特別研究員(PD)   宮崎大学工学部   日本学術振興会特別研究員

    2014.10 - 2016.3

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  • 日本学術振興会特別研究員(DC2)   宮崎大学大学院農学工学総合研究科   日本学術振興会特別研究員

    2014.4 - 2014.9

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Professional Memberships 【 display / non-display

  • アメリカ化学会

  • 日本化学会

  • 高分子学会

  • 繊維学会

  • セルロース学会

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Papers 【 display / non-display

  • Physicochemical Properties of Pyrrolidinium-based Room Temperature Ionic Liquids with Propoxyethyl, Ethoxypropyl, or (Methoxymethoxy)ethyl Group

    YOSHII Kazuki, UTO Takuya

    Electrochemistry   92 ( 4 )   043007 - 043007   2024.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:公益社団法人 電気化学会  

    Room temperature ionic liquids (RTILs) with ether oxygen atoms in the side chain have been investigated for electrochemical applications because they show favorable transport properties compared with alkyl analogs. However, the influence of the position and number of ether oxygen atoms on physical properties has not yet been fully clarified. In the present study, the physicochemical properties of RTILs consisting of bis(trifluoromethanesulfonyl)amide (TFSA<sup>−</sup>) with 1-methy-1-propoxyethylpyrrolidinium (Pyr<sub>1,2O3</sub><sup>+</sup>), 1-methyl-1-ethoxypropylpyrrolidinium (Pyr<sub>1,3O2</sub><sup>+</sup>), and 1-methyl-1-(methoxymethoxy)ethylpyrrolidinium (Pyr<sub>1,2O1O1</sub><sup>+</sup>) was investigated using experimental and computational approach. Pyr<sub>1,2O3</sub>TFSA exhibited the lowest viscosity and highest ionic conductivity. Pyr<sub>1,2O1O1</sub>TFSA with two oxygen atoms in the side chain showed relatively high viscosity, indicating the physicochemical properties were affected sensitively by the position and number of ether oxygen atoms. Furthermore, by comparing with the structural isomers reported so far, we were able to systematically discuss the introduction effect of ether oxygen on the physicochemical properties of RTILs.

    DOI: 10.5796/electrochemistry.23-69154

    CiNii Research

  • Protein Cryoprotectant Ability of the Aqueous Zwitterionic Solution Reviewed

    Takekiyo T., Yamada S., Uto T., Nakayama M., Hirata T., Ishizaki T., Kuroda K., Yoshimura Y.

    Journal of Physical Chemistry B   128 ( 2 )   526 - 535   2024.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Journal of Physical Chemistry B  

    Protein cryopreservation is important for the long-term storage of unstable proteins. Recently, we found that N-acetylglucosaminyltransferase-V (GnT-V) can be cryopreserved in a deep freezer without temperature control using a dilute binary aqueous solution of 3-(1-(2-(2-methoxyethoxy)ethyl)imidazol-3-io)butane-1-carboxylate (OE2imC3C) [10 wt %, mole fraction of solute (x) = 7.75 × 10-3], an artificial zwitterion. However, it is unclear which solvent properties are required in these media to preserve unstable proteins, such as GnT-V. In this study, we investigated the melting phenomena and solution structure of dilute binary aqueous OE2imC3C solutions [x = 0-2.96 × 10-2 (0-30 wt %)] using differential scanning calorimetry (DSC) and Raman and Fourier transform infrared (FTIR) spectroscopies combined with molecular dynamics (MD) simulation to compare the cryoprotectant ability of OE2imC3C with two general cryoprotectants (CPAs), glycerol and dimethyl sulfoxide. DSC results indicated that aqueous OE2imC3C solutions can be melted at lower temperatures with less energy than the control CPA solution, with increasing x, primarily due to OE2imC3C having a higher content of unfrozen water molecules. Moreover, Raman and FTIR results showed that the high content of unfrozen water molecules in aqueous OE2imC3C solutions was due to the hydration around the ionic parts (the COO- group and imidazolium ring) and the OCH2CH2O segment. In addition, the MD simulation results showed that there were fewer structured water molecules around the OCH2CH2O segment than the hydration water molecules around the ionic parts. These solvent properties suggest that dilute aqueous OE2imC3C solutions are effective in preventing freezing, even in a deep freezer. Therefore, this medium has the potential to act as a novel cryoprotectant for proteins in biotechnology and biomedical fields.

    DOI: 10.1021/acs.jpcb.3c05614



  • Optimization of Zwitterionic Polymers for Cell Cryopreservation Reviewed

    Kato Y., Uto T., Ishizaki T., Tanaka D., Ishibashi K., Matsuda Y., Onoda I., Kobayashi A., Hazawa M., Wong R.W., Takahashi K., Hirata E., Kuroda K.

    Macromolecular Bioscience   e2300499   2024

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Macromolecular Bioscience  

    Cryopreservation techniques are valuable for the preservation of genetic properties in cells, and the development of this technology contributes to various fields. In a previous study, an isotonic freezing medium composed of poly(zwitterion) (polyZI) has been reported, which alleviates osmotic shock, unlike typical hypertonic freezing media. In this study, the primitive freezing medium composed of emerging polyZI is optimized. Imidazolium/carboxylate-type polyZI (VimC3C) is the optimal chemical structure. The molecular weight and degree of ion substitution (DSion) are not significant factors. There is an impediment with the primitive polyZI freezing media. While the polyZI forms a matrix around the cell membrane to protect cells, the matrix is difficult to remove after thawing, resulting in low cell proliferation. Unexpectedly, increasing the poly(VimC3C) concentration from 10% to 20% (w/v) improves cell proliferation. The optimized freezing medium, 20% (w/v) poly(VimC3C)_DSion(100%)/1% (w/v) NaCl aqueous solution, exhibited a better cryoprotective effect.

    DOI: 10.1002/mabi.202300499



  • Cell-compatible isotonic freezing media enabled by thermo-responsive osmolyte-adsorption/exclusion polymer matrices Reviewed

    Kato Y., Matsuda Y., Uto T., Tanaka D., Ishibashi K., Ishizaki T., Ohta A., Kobayashi A., Hazawa M., Wong R.W., Ninomiya K., Takahashi K., Hirata E., Kuroda K.

    Communications Chemistry   6 ( 1 )   260   2023.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Communications Chemistry  

    During the long-term storage of cells, it is necessary to inhibit ice crystal formation by adding cryoprotectants. Non-cell-permeable cryoprotectants have high osmotic pressure which dehydrates cells, indirectly suppressing intracellular ice crystal formation. However, the high osmotic pressure and dehydration often damage cells. Emerging polymer-type non-cell-permeable cryoprotectants form matrices surrounding cells. These matrices inhibit the influx of extracellular ice nuclei that trigger intracellular ice crystal formation. However, these polymer-type cryoprotectants also require high osmotic pressure to exert an effective cryoprotecting effect. In this study, we designed a poly(zwitterion) (polyZI) that forms firm matrices around cells based on their high affinity to cell membranes. The polyZI successfully cryopreserved freeze-vulnerable cells under isotonic conditions. These matrices also controlled osmotic pressure by adsorbing and desorbing NaCl depending on the temperature, which is a suitable feature for isotonic cryopreservation. Although cell proliferation was delayed by the cellular matrices, washing with a sucrose solution improved proliferation.

    DOI: 10.1038/s42004-023-01061-7



  • Control of Thermal Diffusivity of Nanocellulose Papers by Introduction of Metal Ions

    UETANI Kojiro, UTO Takuya

    Journal of the Japan Society of Colour Material   96 ( 7 )   228 - 232   2023.7

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Japan Society of Colour Material  

    Crystalline cellulose nanofibers (nanocelluloses) are produced through chemical purification and nanofibrillation of biomass such as ascidian mantles and wood flour. Nanocellulose with carboxy groups introduced on the surface is dried to make a freestanding paper, and when immersed in an aqueous solution of metal chloride, metal ions can be introduced into the nanocellulose paper. The thermal diffusivity of these metal ion-introduced papers was measured in a low-humidity environment and found to vary depending on the ionic radius and electronegativity. This review outlines the recently revealed thermal conductive properties of nanocellulose papers and the control of thermal diffusivity by ion introduction.

    DOI: 10.4011/shikizai.96.228

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Books 【 display / non-display

  • セルロースナノファイバーの調製、分散・複合化と製品応用


    ㈱技術情報協会  2015.1 

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    Responsible for pages:405-411   Language:Japanese


MISC 【 display / non-display

  • コンピュータシミュレーションによる新規セルロース材料の分子設計


    科研費による研究成果展開;九州・沖縄地方成果事例報告書   127   2017.3

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (bulletin of university, research institution)  


    Other Link: http://www.mext.go.jp/a_menu/shinkou/hojyo/1372562.htm

Awards 【 display / non-display

  • 2018年度繊維学会奨励賞

    2019.6   公益社団法人 繊維学会   構造多糖材料の結晶構造特性と溶解機構に関する計算化学研究

    宇都 卓也

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    Award type:International academic award (Japan or overseas)  Country:Japan

Grant-in-Aid for Scientific Research 【 display / non-display

  • 糖鎖相互作用の機構解明を目指した新規な分子力場の開発

    Grant number:23K18510  2023.04 - 2026.03

    独立行政法人日本学術振興会  科学研究費補助金  挑戦的研究(萌芽)

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    Authorship:Principal investigator 

  • 木材パルプの解繊度に依存するスペクトル物性の開拓

    Grant number:22H02407  2022.04 - 2025.03

    独立行政法人日本学術振興会  科学研究費補助金  基盤研究(B)

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  • 分子シミュレーションとデータ科学手段の連携による多糖誘導体キラル分離機構の解明

    Grant number:21K05187  2021.04 - 2024.03

    独立行政法人日本学術振興会  科学研究費補助金  基盤研究(C)

    湯井 敏文、

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  • 計算化学とデータ科学の融合による構造多糖材料における界面メカニズムの解明

    Grant number:20K15232  2020.04 - 2023.03

    独立行政法人日本学術振興会  科学研究費補助金  若手研究

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    Authorship:Principal investigator 


  • 糖結合モジュールタンパク質による糖鎖基質認識機構の網羅的解析

    Grant number:17K00409  2017.04 - 2020.03

    科学研究費補助金  基盤研究(C)

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    孤立分子鎖または結晶状態のセルロースやキチン糖鎖基質と可逆的(非触媒的)に結合する糖結合モジュール(CBM)タンパク質を対象とした。CBMは触媒能力を持たないが、触媒ドメインの基質に対するアクセシビリティを向上させる。酵素本体(触媒ドメイン)に基質が活性部位に結合した複合体の場合、結晶構造解析対象となることが多いが、可逆的かつ多様な結合状態が予想されるCBM-糖鎖複合体の結晶構造解析研究例は限られている。本研究は、コンピュータによる分子シミュレーションを手段として、CBMの基質認識機構を明らかにすることを目的とした。CBMは糖鎖に対する結合様式によって、主に不溶・結晶状態の糖鎖に対してCBM表面が認識する様式(Type A)、CBM表面の浅いクレフト上で孤立糖鎖を認識する様式(Type B)、および糖1~2残基程度の糖鎖部分に対してCBM表面が認識する様式(Type C)に分類される。これらの三つの結合様式に対応する複数のCBMをモデル系として選択し、それらCBMと糖鎖基質の複合体構造モデル群を網羅的に求める。得られた複合体モデル群のシミュレーション計算を実施し、基質認識に関わる熱力学量や立体構造を解析する。さらに、CBM表面に対する糖鎖基質の結合・脱離過程や結合クレフト上の基質移動過程等のより大きな構造変化を伴う基質認識過程を、分子シミュレーション計算によって再現する。以上の知見から、CBMが示す可逆的かつ多様な糖鎖基質認識機構の空間的・時間的な全体像を明らかにする。一般に、糖加水分解酵素(セルラーゼ、キチナーゼ等)は、CBMを失うと触媒活性を大きく低下する。本研究の成果は、酵素全体の反応機構に理解に貢献することに加え、加水分解活性や基質認識特性を制御する変異CBM設計へと展開することが期待される。

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Committee Memberships 【 display / non-display

  • 文部科学省科学技術・学術政策研究所   科学技術予測センター専門調査員  


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    Committee type:政府

  • 高分子学会   九州地区高分子若手研究会 幹事  

    2019.4 - 2020.3   

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    Committee type:学協会

  • 繊維学会   繊維学会西部支部 第2回若手講演会 世話人  


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    Committee type:学協会

Social Activities 【 display / non-display

  • 宮崎県理科・化学教育懇談会 役員

    Role(s): Planner, Organizing member

    2019.10.1 - Now