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医学部 医学科 解剖学講座組織細胞化学分野 |
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助教 |
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宮崎市清武町木原5200 |
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甲斐 健吾 (カイ ケンゴ)
KAI Kengo
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学内職務経歴 【 表示 ／ 非表示 】
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宮崎大学 医学部 医学科 解剖学講座組織細胞化学分野 助教
2024年04月 - 継続中
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宮崎大学 医学部 附属病院 消化管・内分泌・小児外科 助教
2020年04月 - 2024年03月
所属学協会 【 表示 ／ 非表示 】
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日本光線力学学会
2019年 - 現在
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日本組織細胞化学会
2019年 - 現在
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日本内視鏡外科学会
2018年 - 現在
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日本腹部救急医学会
2018年 - 現在
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日本大腸肛門病学会
2018年 - 現在
論文 【 表示 ／ 非表示 】
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Kai K, Hiyoshi M, Imamura N, Hamada T, Yano K, Sato Y, Sakae T, Komi M, Nakamura T, Choijookhuu N, Hishikawa Y, Nanashima A
Internal medicine (Tokyo, Japan) 62 ( 8 ) 1107 - 1115 2023年
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Internal Medicine
Objective The extracellular volume (ECV) calculated based on contrast-enhanced computed tomography (CT) has been reported as a novel imaging parameter reflecting the morphological change of fibrosis in several parenchymal organs. Our retrospective study assessed the validity of the ECV fraction for diagnosing pancreatic fibrosis and the appropriate imaging condition as the “equilibrium phase”. Methods In 27 patients undergoing multiphasic CT and subsequent pancreaticoduodenectomy, we investigated pathological fibrotic changes related to the ECV fraction and conducted analyses using the value obtained by subtracting the equilibrium CT value of the portal vein from that of the abdominal aorta (AoPVequilibrium) to estimate eligibility of the equilibrium phase. Results In all patients, the ECV fraction showed a weak positive correlation with the collagenous compartment ratio (r=0.388, p=0.045). All patients were divided into two groups - the high-Ao-PVequilibrium group and low-Ao-PVequilibrium group - based on the median value. No significant correlation was found in the high-Ao-PVequilibrium group, whereas a significant correlation was observed in the low-Ao-PVequilibrium group (r=0.566, p= 0.035). Conclusion The ECV fraction is a possible predictive factor for histopathological pancreatic fibrosis. In its clinical application, the eligibility of the “equilibrium phase” may affect the diagnostic capability. It will be necessary to verify the imaging conditions in order to improve the accuracy of the diagnosis.
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Non-Cytotoxic Photodynamic Therapy with Talaporfin Sodium Reduces the Expression of CXCR4 and Enhances Chemotherapeutic Efficacy in Undifferentiated Gastric Cancer Cell Line HGC27 査読あり
Kai Kengo, Ishizuka Takumi, Matsumoto Jin, Shimamawari Koki, Mori Ryoma, Fidya, Lkham-Erdene Baljinnyam, Kubota Toshiki, Ikenoue Makoto, Higuchi Kazuhiro, Nanashima Atsushi, Hishikawa Yoshitaka
Ａｃｔａ Ｈｉｓｔｏｃｈｅｍｉｃａ ｅｔ Ｃｙｔｏｃｈｅｍｉｃａ 58 ( 2 ) 69 - 79 2025年4月
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：日本組織細胞化学会
Gastric cancer (GC), particularly the undifferentiated type, is frequently associated with peritoneal metastasis, which significantly worsens prognosis due to its resistance to conventional treatments. Photodynamic therapy (PDT) is localized treatment using a photosensitizer (PS) activated by light of a specific wavelength to generate cytotoxic reactive oxygen species that induce cell death. Severe adverse events were reported from clinical trials investigating PDT for peritoneal dissemination conducted until the early 2000s, leaving its safety and clinical effectiveness unestablished. The present study explored whether “non-cytotoxic” PDT using talaporfin sodium (TS) could enhance efficacy of chemotherapeutic agents in undifferentiated GC cell line HGC27. Cell viability was evaluated with MTT assay following TS-PDT, and the synergistic effect between non-cytotoxic TS-PDT and anticancer drug SN-38 was assessed. Changes in expression of drug resistance markers were analyzed through qRT-PCR, Western blotting, and immunocytochemistry. We found that non-cytotoxic TS-PDT enhanced the efficacy of chemotherapy in the undifferentiated GC cell line and reduced the expression of C-X-C chemokine receptor type 4, a key marker associated with GC stem-like properties. These findings highlight the potential of non-cytotoxic TS-PDT as a synergistic treatment approach. We conclude that non-cytotoxic TS-PDT could enhance drug sensitivity and offers a promising therapeutic strategy for GC.
DOI： 10.1267/ahc.25-00002
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Higuchi K, Ikenoue M, Ishizuka T, Kai K, Takahashi N, Kubota T, Shirouzu S, Lkham-Erdene B, Aung KM, Nakai M, Sawaguchi A, Nanashima A, Hishikawa Y
Acta histochemica et cytochemica 58 ( 1 ) 9 - 18 2025年2月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：日本組織細胞化学会
SET domain bifurcated 1 (SETDB1), a histone H3K9-specific methyltransferase, is crucial for heterochromatin formation and intestinal homeostasis, but its role in intestinal ischemia-reperfusion injury (IRI) remains unclear. This study investigated changes in SETDB1-mediated nuclear chromatin regulation in intestinal epithelial cells (IECs) using an IRI mouse model. Jejunal samples were collected after 75 min of ischemia followed by 24 hr of reperfusion. Sinefungin was administered as a histone methyltransferase inhibitor. Morphologic changes were evaluated using hematoxylin-eosin staining and electron microscopy, and cell-adhesion molecule expression, including ZO-1, E-cadherin, integrin-β4, and laminin, was evaluated using immunohistochemistry. Super-resolution microscopy analyzed intranuclear SETDB1 localization and heterochromatin formation in IECs. IRI-affected jejunum exhibited massive IEC detachment, dilated intercellular spaces, basement membrane damage, and decreased expression of E-cadherin and integrin-β4. Sinefungin prevented these changes, however. The proportion of IECs expressing nuclear SETDB1 throughout the euchromatin was significantly higher in IRI-affected jejunum (77.8%) than sham-treated (3.0%) or sinefungin-treated, IRI-affected jejunum (2.7%). The proportion of IECs with decreased heterochromatin was significantly higher in sinefungin-treated, IRI-affected jejunum (84.3%) than untreated IRI-affected jejunum (15.6%). These findings suggest that SETDB1-mediated chromatin regulation is pivotal in intestinal IRI and represents a potential therapeutic target.
DOI： 10.1267/ahc.24-00061
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Nanashima A., Hiyoshi M., Imamura N., Hamada T., Tsuchimochi Y., Shimizu I., Ochiai T., Ichihara A., Hamada K., Ichiki N., Kai K., Higuchi K.
Translational Cancer Research 14 ( 1 ) 461 - 472 2025年1月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Translational Cancer Research
Background: Progression of chronic liver dysfunction influenced by long-term chemotherapy or repeated hepatectomy might be related to patient overall survival as well as tumor factors in colorectal liver metastasis (CLM) patients. Our aim of this study was to clarify the relationship between fibrotic liver damage and malignant behaviors of CLM malignancy or its long-term survivals by the retrospective cohort study. Methods: We examined the relationship between tumor-related factors or six liver fibrosis-associated parameters, including platelet count, hyaluronic acid (HA), mac-2 binding protein glycosylation isomer (M2BPGi), type IV collagen 7S (T4C7), aspartate aminotransferase-to-platelet ratio index (APRI), The fibrosis-4 (Fib-4) index, and clinicopathological parameters, surgical records, and postoperative patient survival in the 45 consecutive patients with CLM who underwent radical hepatectomy. Results: Fibrotic parameters were platelet count of 23.0±8.5 ×104/μL, HA level of 68.9±82.3 ng/mL, M2BPGi of 0.87±0.48 ng/mL, and type IV collagen level of 5.74±3.76 ng/mL. Platelet count was significantly correlated with HA level (P<0.05) and tended to be correlated with M2BPGi levels (P=0.056). HA level was significantly associated with albumin level (P<0.05). Overall survival in this series showed five-year overall survivors after hepatectomy in 44 patients (98%), but cancer-related deaths were observed in only one patient. Patients with higher grades and increased bilirubin levels demonstrated significantly lower cancer-free survival (P<0.05), but fibrotic parameters were not associated with prognostic factors. Conclusions: Fibrotic markers indicating chemotherapy or repeated surgical liver injury were not significant predictive factors reflecting cancer malignant behaviors or patient overall survival, contrary to our hypothesis. The current overall survival status using various modalities for cancer recurrence is satisfactory under our present perioperative management.
DOI： 10.21037/tcr-24-1138
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Hosokawa A., Tamura H., Ichihara A., Imamura N., Kai K., Fukushima T., Nanashima A., Komohara Y.
Anticancer Research 44 ( 9 ) 4119 - 4125 2024年9月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Anticancer Research
Background/Aim: Immune checkpoint inhibitors are effective in treating microsatellite instability-high (MSI-H) metastatic colorectal cancer (CRC). Pathological complete response to immune checkpoint inhibitors for MSI-H metastatic CRC have been described in several reports. Liver metastasis is known to predict resistance to ani-programmed death 1 (PD-1)/PD-1 ligand 1 (PD-L1) therapy in several cancers, including CRC. Case Report: Herein, we report the case of a 23-year-old man with MSI-H colorectal liver metastasis who exhibited a pathological complete response to pembrolizumab following systemic chemotherapies. Pathological examination of the primary lesion revealed strong HLA-class I and HLA-DR expression in cancer cells. Elevated PD-L1 expression was observed in areas of increased CD8-postive T cell infiltration. Additional pathological study of regional lymph nodes showed increased PD-L1 and CD169 expression. Conclusion: A detailed pathological examination revealed PD-L1 expression not only in the primary CRC lesion but also in regional lymph nodes. Recent studies have highlighted the significance of regional lymph nodes in anti-cancer immune responses. Therefore, pathological studies using resected lymph nodes might be beneficial for predicting the response of anti-PD-1/PD-L1 therapy.
書籍等出版物 【 表示 ／ 非表示 】
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Introduction to the h-principle
Cieliebak Kai, Eliashberg Y., Mishachev N. (Nikolai M.)（ 担当： 単著）
American Mathematical Society 2024年 （ ISBN:9781470461058 ）
記述言語：英語
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Research handbook on law and psychology
Hollander-Blumoff Rebecca, Bornstein Brian H., Tyler Tom R., Mitchell Gregory (Law teacher), Maroney Terry A, Goya-Tocchetto Daniela, Ponce de Leon Rebecca, Jost John T., Spruill Mikaela, Hans Valerie P., Grosso Catherine M., O'Brien Barbara, Sevier Justin, MacCoun Robert J., Pustilnik Amanda, Madon Stephanie, Scherr Kyle C, Ditchfield Ryan, Dysart Jennifer E, Haney-Caron Emily, Ramos Lili, Tyler Nichola, Gannon Theresa A, Robbennolt Jennifer K（ 担当： 単著）
Edward Elgar Publishing 2024年 （ ISBN:9781800881914 ）
記述言語：英語
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Introduction to the h-principle
Cieliebak Kai, Eliashberg Y., Mishachev N. (Nikolai M.)（ 担当： 単著）
American Mathematical Society 2024年 （ ISBN:9781470476175 ）
記述言語：英語
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Borre K. (Kai), Fernández-Hernández Ignacio, López-Salcedo José A., Bhuiyan M. Zahidul H.（ 担当： 単著）
Cambridge University Press 2023年 （ ISBN:9781108837019 ）
記述言語：英語
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Student engagement in the language classroom
Hiver Phil, Al-Hoorie Ali H., Mercer Sarah（ 担当： 単著）
Multilingual Matters 2021年 （ ISBN:9781788923606 ）
記述言語：英語
MISC 【 表示 ／ 非表示 】
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Shirouzu S., Sugita N., Choijookhuu N., Yamaguma Y., Takeguchi K., Ishizuka T., Tanaka M., Fidya , Kai K., Chosa E., Yamashita Y., Koshimoto C., Hishikawa Y.
Journal of Ovarian Research 16 ( 1 ) 21 2023年12月
記述言語：英語 掲載種別：速報，短報，研究ノート等（学術雑誌） 出版者・発行元：Journal of Ovarian Research
Following publication of the original article [1], the uthors identified an error in authorgroup. Author name Fidya Fidya should be Fidya. The original article has been corrected.
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Rare resected eight cases of duodenal adenocarcinomas
Nanashima A., Tanoue Y., Imamura N., Hiyoshi M., Yano K., Hamada T., Nishida T., Kai K., Suzuki Y., Sato Y., Nakashima K., Hosokawa A., Nagayasu T.
International Journal of Surgery Case Reports 86 106384 2021年9月
記述言語：日本語 掲載種別：速報，短報，研究ノート等（学術雑誌） 出版者・発行元：International Journal of Surgery Case Reports
Introduction: Duodenal adenocarcinoma is a rare malignancy; recently, it has been found to be accompanied by operative indications. Methods: Nine consecutive rare cases were diagnosed with duodenal carcinoma (DC), in which clinicopathological characteristics were retrospectively examined. Age was ranged over middle-aged males and females. No clinical onset with severe symptoms was observed, and the specific treatment for accompanied diseases or habits was not found. Outcomes: One case of two T1 stage DCs that underwent pancreas-sparing duodenectomy. Stage II DC was diagnosed in three cases, and stage III DC was diagnosed in four cases. Pancreaticoduodenectomy (PD) mainly occurred in seven patients, and duodenectomy was limited in two patients. All operations were safely performed, and the postoperative course showed no severe morbidity. Histological findings showed R0 resection in eight cases and R1 at the retroperitoneal dissecting part in one case. Five patients with advanced-stage DC underwent adjuvant chemotherapy; however, four patients showed tumor recurrence within 12 months. With additional strong chemotherapy, eight patients survived up to 84 months, and one died of liver metastasis at 43 months after surgery. Three representative cases of mucosal invasion with widespread pancreas-sparing duodenectomy and advanced-stage DC cases undergoing duodenectomy or PD are shown. Conclusion: In the field of upper digestive tract surgery, duodenal adenocarcinoma and various applications of surgery or adjuvant chemotherapy for long-term survival are important.
講演・口頭発表等 【 表示 ／ 非表示 】
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A Clinicopathological Study of Extracellular Volume Fraction Determined by Contrast Enhanced Computed Tomography as a Predictive Factor of Pancreatic Fistula after Pancreaticoduodenectomy 国際会議
Kai K, Hiyoshi M, Imamura N, Hamada T, Yano K, Sato Y, Sakae T, Choijookhuu N, Hishikawa Y, Nanashima A
15th IHPBA World Congress
開催年月日： 2022年3月30日 - 2022年4月2日
会議種別：ポスター発表
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当院における妊娠合併虫垂炎に対する 腹腔鏡下虫垂切除術の経験と工夫
甲斐健吾，池田拓人，市原明子，河野文彰，濱田朗子，松澤聡，鮫島浩，七島篤志
第13回Acute care surgery学会学術集会
開催年月日： 2021年11月4日 - 2021年11月27日
会議種別：シンポジウム・ワークショップ パネル（公募）
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膵頭十二指腸切除術における術後膵液漏の発生と造影CTによる細胞外血管外腔算出値の関連
甲斐健吾，旭吉雅秀，矢野公一，今村直哉，濱田剛臣，佐藤勇一朗，榮建文，七島篤志
JDDW 2021 KOBE
開催年月日： 2021年11月4日 - 2021年11月7日
会議種別：口頭発表（一般）
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当科における大腸癌肝転移に対する初回肝切除の治療成績と血清CRP・アルブミン比の意義
甲斐健吾，濱田剛臣，池田拓人，市原明子，今村直哉，旭吉雅秀，内勢由佳子，原大介，鈴木康人，七島篤志
第46回日本大腸肛門病学会九州地方会
開催年月日： 2021年10月16日
会議種別：シンポジウム・ワークショップ パネル（公募）
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当院での経腹壁的エラストグラフィによる膵線維化診断能の病理学的検証
甲斐健吾，濱田剛臣，旭吉雅秀，矢野公一，今村直哉，佐藤勇一朗，河上洋，田島栄美，七島篤志
日本超音波医学会第31回九州地方会学術集会
開催年月日： 2021年10月3日
会議種別：口頭発表（一般）
産業財産権 【 表示 ／ 非表示 】
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制御装置及び制御方法
甲斐 健吾、濵田 剛臣
出願人：宮崎大学
出願番号：2023-028322 出願日：2023年2月27日
公開番号：2024-121297 公開日：2024年9月6日
出願国：国内
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直腸固有筋膜近接神経検査方法、及び神経検査装置
甲斐 健吾
出願人：宮崎大学
出願番号：2022-100239 出願日：2022年6月22日
公開番号：2024-001530 公開日：2024年1月10日
出願国：国内
科研費（文科省・学振・厚労省）獲得実績 【 表示 ／ 非表示 】
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PDP効果に基づく胃癌腹膜播種に対する簡便かつ安全な光治療の開発
研究課題/領域番号：24K11937 2024年04月 - 2027年03月
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
担当区分：研究代表者
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食道癌に対する光線力学療法による薬剤耐性軽減効果の証明と新規治療開発に向けた検証
研究課題/領域番号：20K17694 2020年04月 - 2023年03月
独立行政法人日本学術振興会 科学研究費補助金 若手研究
担当区分：研究代表者
研究・技術シーズ 【 表示 ／ 非表示 】
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低強度光線力学療法を用いた抗癌剤耐性の軽減効果の実証
Photodaynamic primingを用いた新規腹膜播種治療の開発技術相談に応じられる関連分野：培養細胞を用いた光線力学療法の実施
メッセージ：臨床経験から着想を得た研究で、実現すれば多くの癌患者さんの助けになると考えています。少しでも前に進めていけるように、多分野から様々なアドバイスを頂ければと思い掲載を希望しました。