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Faculty of Medicine School of Medicine |
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SUGIYAMA Hironobu
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Papers 【 display / non-display 】
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Inagaki H., Goto Y., Sugiyama H., Saito A., Okabayashi T., Watanabe K., Fujimoto S.
Frontiers in Public Health 10 1053729 2022.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Frontiers in Public Health
Reliable disinfection and sterilization technologies are needed to deal with the various infectious diseases spreading around the world. Furthermore, bacteria that are difficult to eliminate by ordinary disinfection are also a problem in the medical environment. We examined the germicidal effect of a newly developed deep-ultraviolet light-emitting diode (DUV-LED) prototype device (wavelength of 280 ± 5 nm; power of 0.9 to 1.4 mW/cm2) for floor sterilization against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Mycobacterium gordonae (M. gordonae), and Bacillus subtilis (B. subtilis). This prototype device is equipped with highly practical DUV-LEDs with a high output efficiency and a long life, and was designed with consideration of the irradiation distance and the angle of the DUV-LEDs to provide a uniform irradiation rate. We found a statistically significant reduction of ≥90% in the infectious titers of both E. coli and S. aureus after irradiation for 2 s. Although acid-fast bacilli and spore-type bacilli are generally thought to be resistant to UV light irradiation compared to general bacteria, the acid-fast bacillus M. gordonae was inactivated after irradiation for 10 s, and spore-type cells of the bacillus B. subtilis were inactivated by ≥90% after irradiation for 30 s. We also found that the effects were cumulative when irradiation was performed at intervals. In the future, the usefulness of this device as an infection control measure will be evaluated in daily medical practice.
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Kaneko C., Saito A., Inagaki H., Sugiyama H., Mazimpaka E., Fujimoto S., Okabayashi T.
Journal of Medical Virology 94 ( 7 ) 3438 - 3441 2022.7
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Journal of Medical Virology
Severe fever with thrombocytopenia syndrome (SFTS) caused by Dabie bandavirus (SFTSV) is a serious public health concern in endemic areas, particularly in Asian and Southeast Asian countries. SFTSV is transmitted by direct contact with body fluids from infected humans and animals. Therefore, environmental hygiene in hospitals and veterinary clinics in SFTSV-endemic areas is highly important. This study assessed the effects of continuous and intermittent irradiation with deep-ultraviolet light-emitting diode (DUV-LED) on SFTSV. Evaluation was performed by conducting plaque assay in which SFTSV irradiated with deep-ultraviolet (DUV; 280 ± 5 nm) was inoculated onto Vero cells. The results showed that continuous and intermittent irradiation for 5 s, resulting in 18.75 mJ/cm2 of cumulative UV exposure, led to a >2.7 and >2.9 log reduction, respectively, corresponding to a >99.8% reduction in infectivity. These results demonstrate that DUV can be utilized for inactivation of SFTSV to maintain environmental hygiene in hospitals and veterinary clinics in endemic countries.
DOI： 10.1002/jmv.27698
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Inagaki H., Saito A., Kaneko C., Sugiyama H., Okabayashi T., Fujimoto S.
Pathogens 10 ( 6 ) 2021.6
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Pathogens
More than 1 year has passed since social activities have been restricted due to the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). More recently, novel SARS-CoV-2 variants have been spreading around the world, and there is growing concern that they may have higher transmissibility and that the protective efficacy of vaccines may be weaker against them. Immediate measures are needed to reduce human exposure to the virus. In this study, the antiviral efficacy of deep-ultraviolet light-emitting diode (DUV-LED) irradiation (280 ± 5 nm, 3.75 mW/cm2 ) against three SARS-CoV-2 variants was evaluated. For the B.1.1.7, B.1.351, and P.1 variant strains, irradiation of the virus stocks for 1 s resulted in infectious titer reduction rates of 96.3%, 94.6%, and 91.9%, respectively, and with irradiation for 5 s, the rates increased to 99.9%, 99.9%, and 99.8%, respectively. We also tested the effect of pulsed DUV-LED irradiation (7.5 mW/cm2, duty rate: 50%, frequency: 1 kHz) under the same output conditions as for continuous irradiation and found that the antiviral efficacy of pulsed and continuous irradiation was the same. These findings suggest that by further developing and optimizing the DUV-LED device to increase its output, it may be possible to instantly inactivate SARS-CoV-2 with DUV-LED irradiation.
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Rapid Inactivation of SARS-CoV-2 with Ozonated Water Reviewed
Inagaki H., Saito A., Sudaryatma P.E., Sugiyama H., Okabayashi T., Fujimoto S.
Ozone: Science and Engineering 43 ( 3 ) 208 - 212 2021
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Ozone: Science and Engineering
Although ozonated water is one of the promising candidates for hand hygiene to prevent fomite infection, the detailed effects of ozonated water on SARS-CoV-2 have not been clarified. We evaluated the inactivating effect of ozonated water against SARS-CoV-2 by its concentration and exposure time. The reduction rates of virus titer after 5 sec treatment with ozone concentrations of 1, 4, 7, and 10 mg/L were 81.4%, 93.2%, 96.6%, and 96.6%, respectively. No further decrease in virus titer was observed by the extended exposure time over 5 sec. High-concentration ozonated water, such as more than 7 mg/L, was considered to be effective in promptly inactivating SARS-CoV-2 virus in aqueous solution.
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Rapid inactivation of SARS-CoV-2 with deep-UV LED irradiation Reviewed
Hiroko Inagaki, Akatsuki Saito, Hironobu Sugiyama, Tamaki Okabayashi ＆ Shouichi Fujimoto
Emerging Microbes ＆ Infections 2020
Publishing type：Research paper (scientific journal)
Books 【 display / non-display 】
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深紫外線LED照射によるウイルス不活化と製品開発への応用
杉山博信（ Role： Sole author）
研究開発リーダー月刊2021.4 2021
Book type：Scholarly book
Presentations 【 display / non-display 】
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室内環境の並列流体解析の基本検討
諸井翔, 杉山博信, 藤元昭一, 武居周
第35回計算力学講演会 2022.11.16
Event date： 2022.11.16 - 2022.11.18
Presentation type：Oral presentation (general)
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Non-DEHP血液回路
杉山博信
徳島県臨床工学技士会学術集会 2004
Event date： 2004
Presentation type：Oral presentation (general)
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事故防止を目的とした血液回路について Invited
杉山博信
日本血液浄化技術研究会 2003
Event date： 2003
Presentation type：Oral presentation (general)
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新時代の血液回路について
杉山博信
岩手県臨床工学技士会 2003
Event date： 2003
Presentation type：Oral presentation (general)
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Non-DEHP血液回路
杉山博信
新潟県臨床工学技士会 2003
Event date： 2003
Presentation type：Oral presentation (general)
Awards 【 display / non-display 】
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2022年日本オゾン協会論文賞
2022 日本オゾン協会
稲垣浩子、杉山博信、Putu Eka Sudaryatma, 齊藤暁、岡林環樹、藤元昭一
Award type：Award from Japanese society, conference, symposium, etc.
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2021年日本オゾン協会技術賞
2021 日本オゾン協会
稲垣浩子、杉山博信、Putu Eka Sudaryatma, 齊藤暁、岡林環樹、藤元昭一
Award type：Award from Japanese society, conference, symposium, etc.