KURODA Kohei

写真a

Affiliation

Faculty of Agriculture Department of Veterinary Science

Title

Assistant Professor

External Link

Degree 【 display / non-display

  • 博士(獣医学) ( 2018.3   山口大学 )

Research Areas 【 display / non-display

  • Life Science / Veterinary medical science

 

Papers 【 display / non-display

  • Novel approach for reconstruction of the three-dimensional biliary system in decellularized liver scaffold using hepatocyte progenitors Reviewed

    Hirukawa K., Yagi H., Kuroda K., Watanabe M., Nishi K., Nagata S., Abe Y., Kitago M., Adachi S., Sudo R., Kitagawa Y.

    PLoS ONE   19 ( 2 February )   e0297285   2024.2

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PLoS ONE  

    Reconstruction of the biliary system is indispensable for the regeneration of transplantable liver grafts. Here, we report the establishment of the first continuous three-dimensional biliary system scaffold for bile acid excretion using a novel method. We confirmed the preservation of the liver-derived extracellular matrix distribution in the scaffold. In addition, hepatocyte progenitors decellularized via the bile duct by slow-speed perfusion differentiated into hepatocyte- and cholangiocyte-like cells, mimicking hepatic cords and bile ducts, respectively. Furthermore, qRT-PCR demonstrated increased ALB, BSEP, and AQP8 expression, revealing bile canaliculi- and bile duct-specific genetic patterns. Therefore, we concluded that locally preserved extracellular matrices in the scaffold stimulated hepatic progenitors and provided efficient differentiation, as well as regeneration of a three-dimensional continuous biliary system from hepatic cords through bile ducts. These findings suggest that organ-derived scaffolds can be utilized for the efficient reconstruction of functional biliary systems.

    DOI: 10.1371/journal.pone.0297285

    Scopus

  • A retrospective analysis for criteria of surgical intervention in Japanese Black calves with respiratory signs caused by perinatal rib fracture Reviewed

    KIM Sueun, NAIK Mrunmayi Vishwanath, KIRINO Yumi, SATOH Hiroyuki, TSUZUKI Nao, INOUE Yoshiyuki, KURODA Kohei, HIDAKA Yuichi

    Journal of Veterinary Medical Science   85 ( 1 )   40 - 43   2022.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:JAPANESE SOCIETY OF VETERINARY SCIENCE  

    Medical records of 16 Japanese Black calves with respiratory signs associated with perinatal rib fracture were analyzed, retrospectively, to speculate criteria of surgical intervention for the disease. For this analysis, the severity of respiratory condition was classified into three grades: Grade 1, with no wheezing, Grade 2, with wheezing after excitement but not at rest and Grade 3, with wheezing at rest. Grade 1 (n=3) received only conservative management. Seven of Grade 2 (n=8) and all of Grade 3 (n=5) had surgical management. Clinical outcome was good in all of Grade 1, and the surgical success rate was 83% in other groups. Our result suggests that surgical intervention should be considered for cases with more the severity of Grade 2.

    DOI: 10.1292/jvms.22-0332

    Scopus

    PubMed

    CiNii Research

  • Intra-abdominal mass containing controlled internal drug release (CIDR) devices detected by CT in a Japanese Black cow Reviewed

    Sato, R. Satoh, H. Okada, S. Goda, Y. Sueun, K. Kuroda, K. Inoue, Y. Kitahara, G. Osawa, T.

    J Vet Med Sci   84 ( 8 )   1088 - 1092   2022.6

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1292/jvms.22-0180

  • Decellularized Organ-Derived Scaffold Is a Promising Carrier for Human Induced Pluripotent Stem Cells-Derived Hepatocytes Reviewed

    Hideaki Kojima Hiroshi Yagi Hiroko Kushige Yukiko Toda Kazuo Takayama Shinako Masuda Toshinori Morisaku Tomonori Tsuchida Kohei Kuroda Kazuya Hirukawa Jumpei Inui Kotaro Nishi Yutaka Nakano Masayuki Tanaka Shutaro Hori Yasushi Hasegawa Yuta Abe Minoru Kitago Shungo Adachi Masatoshi Tomi Katsuhisa Matsuura Hiroyuki Mizuguchi Yuko Kitagawa

    cells   11 ( 8 )   1258   2022.4

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    Publishing type:Research paper (scientific journal)  

  • Identification of domestic cat hepadnavirus from a cat blood sample in Japan Reviewed

    Takahashi, Kazuki Kaneko, Yasuyuki Shibanai, Akiko Yamamoto, Shushi Katagiri, Ayana Osuga, Tatsuyuki Inoue, Yoshiyuki Kuroda, Kohei Tanabe, Mika Okabayashi, Tamaki Naganobu, Kiyokazu Minobe, Isao Saito, Akatsuki

    Journal of Veterinary Medical Science   84 ( 5 )   648 - 652   2022.3

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    Publishing type:Research paper (scientific journal)  

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Presentations 【 display / non-display

  • 自己臓器再生を誘導するためのIn Situ肝部分肝脱細胞化の検討

    黒田晃平、八木洋、田島一樹、蛭川和也、櫛笥博子、北川雄光

    第19回日本再生医療学会総会 

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    Event date: 2020.8.27 - 2020.9.10

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 脱細胞化骨格を用いた腎再生の試み

    黒田晃平, 八木洋, 田島一樹, 蛭川和也, 櫛笥博子, 木下梨恵, 渋谷初子, 北川雄光

    第46回日本臓器保存生物医学会学術集会 

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    Event date: 2019.11.8 - 2019.11.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

  • 脱細胞化骨格を用いた腎臓再生

    黒田晃平, 八木洋, 田島一樹, 蛭川和也, 北川雄光

    第18回日本再生医療学会総会 

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    Event date: 2019.3.21 - 2019.3.23

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 脱細胞化臓器骨格を用いた肝臓再生の試み Invited

    黒田晃平, 八木洋, 東尚伸, 田島一樹, 篠田昌宏, 北川雄光

    第25回肝細胞研究会 

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    Event date: 2018.7.12 - 2018.7.13

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

  • IoT接続トイレ型健康モニターを用いた正常および泌尿器疾患猫の排尿行動解析

    黒田晃平, 石井万寿美, 高野佳子, 伊藤宏康, 大塚健文, 大石正人, 市野邦男, 笹岡直人, 岸田 悟, 東 和生, 大﨑智弘, 柄 武志, 村端悠介, 伊藤典彦, 今川智敬, 岡本芳晴

    平成29年度獣医学術中国地区学会 

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    Event date: 2017.10.14 - 2017.10.15

    Language:English   Presentation type:Oral presentation (general)  

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Grant-in-Aid for Scientific Research 【 display / non-display

  • オキシブプロカイン点眼液のイヌ水疱性角膜症における角膜浮腫改善機序の解明

    Grant number:23K05537  2023.04 - 2026.03

    独立行政法人日本学術振興会  科学研究基金  基盤研究(C)

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    Authorship:Coinvestigator(s) 

  • ALPPSと脱細胞化技術を組み合わせた新規in situ肝再生法の開発

    Grant number:19K18069  2019.04 - 2022.03

    科学研究費補助金  若手研究

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    Authorship:Principal investigator 

  • 犬の色素性角膜炎に対する培養角膜上皮細胞シートを用いた獣医再生医療の試み

    Grant number:17J06460  2017.04 - 2019.03

    独立行政法人日本学術振興会  科学研究費補助金  特別研究員奨励費