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Affiliation |
Organization for Promotion of Research and Industry-Academic Regional Collaboration Institute for Tenure Track Promotion |
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Lecturer |
IWANO satoshi
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Research Areas 【 display / non-display 】
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Nanotechnology/Materials / Bio chemistry
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Life Science / Basic brain sciences
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Nanotechnology/Materials / Chemical biology
Papers 【 display / non-display 】
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Shoichi Kimura, Satoshi Iwano, Takahiro Akioka, Takahiro Kuchimaru, Makiko Kawaguchi, Tsuyoshi Fukushima, Yuichiro Sato, Hiroaki Kataoka, Toshiyuki Kamoto, Shoichiro Mukai, Atsuro Sawada
International Journal of Molecular Sciences 26 ( 5 ) 2308 - 2308 2025.3
Language：English Publishing type：Research paper (scientific journal) Publisher：MDPI AG
The liver is the most lethal metastatic site in castration-resistant prostate cancer (CRPC). Overexpression of MET protein has been reported in CRPC, and MET is an important driver gene in androgen-independent CRPC cells. Mouse CRPC cell line CRTC2 was established by subcutaneous injection of hormone-sensitive PC cells (TRAMP-C2) in castrated nude mice. CRCT2/luc2 cells were injected into the spleen of castrated nude mice, and liver metastasis was confirmed at 2 weeks post-injection. We administered MET inhibitor (MET-I) and HGF activator inhibitor (HGFA-I) to this liver metastasis model and assessed the therapeutic effect. After intrasplenic injection, CRTC2 showed a higher incidence of liver metastasis whereas no metastasis was observed in TRAMP-C2. Microarray analysis revealed increased expression of HGF, MET, and HPN, HGFAC (encoding HGF activating proteases) in liver metastasis. Proliferation of CRCT2 was significantly inhibited by co-administration of MET-I and HGFA-I by in vitro analysis with HGF-enriched condition. In an analysis of the mouse model, the combination-therapy group showed the strongest reduction for liver metastasis. Immunohistochemical staining also revealed the strongest decrease in phosphorylation of MET in the combination-therapy group. Co-culture with HGF-expressed mouse fibroblasts showed attenuation of the inhibitory effect of MET-I; however, additional HGFA-I overcame the resistance. We established an androgen-independent CRPC cell line, CRTC2, and liver metastasis model in mice. Significant effect was confirmed by combined treatment of MET-I and HGFA-I by in vitro and in vivo analysis. The results suggested the importance of combined treatment with both MET- and HGF-targeting agents in the treatment of HGF-enriched conditions including liver metastasis.
DOI： 10.3390/ijms26052308
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Lonidamine, a Novel Modulator for the BvgAS System of Bordetella Species. Reviewed International journal
Natsuko Ota, Takashi Nishida, Daron M Standley, Aalaa Alrahman Sherif, Satoshi Iwano, Dendi Krisna Nugraha, Toshiya Ueno, Yasuhiko Horiguchi
Microbiology and immunology 69 ( 3 ) 133 - 147 2025.3
Language：English Publishing type：Research paper (scientific journal)
The Gram-negative bacteria Bordetella pertussis, B. parapertussis, and B. bronchiseptica cause respiratory diseases in various mammals. They share the BvgAS two-component system, which regulates the phenotypic conversion between the virulent Bvg+ and avirulent Bvg- phases. In the BvgAS system, the sensor kinase BvgS senses environmental cues and transduces a phosphorelay signal to the response regulator BvgA, which leads to the expression of Bvg+ phase-specific genes, including virulence factor genes. Bacteria grown at 37°C exhibit the Bvg+ phenotype. In contrast, at lower than 26°C or in the presence of modulators, such as MgSO4 and nicotinic acid, the BvgAS system is inactivated, leading bacteria to the avirulent Bvg- phase. Therefore, effective modulators are expected to provide a therapeutic measure for Bordetella infection; however, no such modulators are currently available, and the mechanism by which modulators inactivate the BvgAS system is poorly understood. In the present study, we identified lonidamine as a novel modulator after screening an FDA-approved drug library using bacterial reporter systems with the Bvg+-specific and Bvg--specific promoters. Lonidamine directly bound to the VFT2 domain of BvgS and inactivated the BvgAS system at concentrations as low as 50 nM, which was at least 2000- to 20,000-fold lower than the effective concentrations of known modulators. Lonidamine significantly reduced the adherence of B. pertussis to cultured cells but unexpectedly exacerbated bacterial colonization of the mouse nasal septum. These results provide insights into the structural requirements for BvgAS modulators and the role of Bvg phenotypes in the establishment of infection.
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Progenitor effect in the spleen drives early recovery via universal hematopoietic cell inflation. Reviewed International journal
Takao Yogo, Hans Jiro Becker, Takaharu Kimura, Satoshi Iwano, Takahiro Kuchimaru, Atsushi Miyawaki, Tomomasa Yokomizo, Toshio Suda, Atsushi Iwama, Satoshi Yamazaki
Cell reports 44 ( 2 ) 115241 - 115241 2025.2
Language：English Publishing type：Research paper (scientific journal)
Hematopoietic stem cells (HSCs) possess the capacity to regenerate the entire hematopoietic system. However, the precise HSC dynamics in the early post-transplantation phase remain an enigma. Clinically, the initial hematopoiesis in the post-transplantation period is critical, necessitating strategies to accelerate hematopoietic recovery. Here, we uncovered the spatiotemporal dynamics of early active hematopoiesis, "hematopoietic cell inflation," using a highly sensitive in vivo imaging system. Hematopoietic cell inflation occurs in three peaks in the spleen after transplantation, with common myeloid progenitors (CMPs), notably characterized by HSC-like signatures, playing a central role. Leveraging these findings, we developed expanded CMPs (exCMPs), which exhibit a gene expression pattern that selectively proliferates in the spleen and promotes hematopoietic expansion. Moreover, universal exCMPs supported early hematopoiesis in allogeneic transplantation. Human universal exCMPs have the potential to be a viable therapeutic enhancement for all HSC transplant patients.
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Akaluc bioluminescence offers superior sensitivity to track in vivo dynamics of SARS-CoV-2 infection Reviewed
Tomokazu Tamura, Hayato Ito, Shiho Torii, Lei Wang, Rigel Suzuki, Shuhei Tsujino, Akifumi Kamiyama, Yoshitaka Oda, Masumi Tsuda, Yuhei Morioka, Saori Suzuki, Kotaro Shirakawa, Kei Sato, Kumiko Yoshimatsu, Yoshiharu Matsuura, Satoshi Iwano, Shinya Tanaka, Takasuke Fukuhara
iScience 27 ( 5 ) 109647 - 109647 2024.5
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV
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Misa Minegishi, Takahiro Kuchimaru, Kaori Nishikawa, Takayuki Isagawa, Satoshi Iwano, Kei Iida, Hiromasa Hara, Shizuka Miura, Marika Sato, Shigeaki Watanabe, Akifumi Shiomi, Yo Mabuchi, Hiroshi Hamana, Hiroyuki Kishi, Tatsuyuki Sato, Daigo Sawaki, Shigeru Sato, Yutaka Hanazono, Atsushi Suzuki, Takahide Kohro, Tetsuya Kadonosono, Tomomi Shimogori, Atsushi Miyawaki, Norihiko Takeda, Hirofumi Shintaku, Shinae Kizaka-Kondoh, Satoshi Nishimura
Nature Communications 14 ( 1 ) 8031 2023.12
Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC
Abstract
Cancer cells inevitably interact with neighboring host tissue-resident cells during the process of metastatic colonization, establishing a metastatic niche to fuel their survival, growth, and invasion. However, the underlying mechanisms in the metastatic niche are yet to be fully elucidated owing to the lack of methodologies for comprehensively studying the mechanisms of cell–cell interactions in the niche. Here, we improve a split green fluorescent protein (GFP)-based genetically encoded system to develop secretory glycosylphosphatidylinositol-anchored reconstitution-activated proteins to highlight intercellular connections (sGRAPHIC) for efficient fluorescent labeling of tissue-resident cells that neighbor on and putatively interact with cancer cells in deep tissues. The sGRAPHIC system enables the isolation of metastatic niche-associated tissue-resident cells for their characterization using a single-cell RNA sequencing platform. We use this sGRAPHIC-leveraged transcriptomic platform to uncover gene expression patterns in metastatic niche-associated hepatocytes in a murine model of liver metastasis. Among the marker genes of metastatic niche-associated hepatocytes, we identify Lgals3, encoding galectin-3, as a potential pro-metastatic factor that accelerates metastatic growth and invasion.DOI： 10.1038/s41467-023-43855-2
Other Link： https://www.nature.com/articles/s41467-023-43855-2
Presentations 【 display / non-display 】
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生物発光を利用したバイオイメージング技術の開発 Invited
岩野 智
第33回日本バイオイメージング学会 2024.9.29
Event date： 2024.9.28 - 2024.9.30
Language：Japanese Presentation type：Oral presentation (invited, special)
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生物発光を利用したバイオイメージング技術の開発 Invited
岩野 智
革新脳分科会The Final 2024.3.18
Event date： 2024.3.17 - 2024.3.18
Language：Japanese Presentation type：Oral presentation (general)
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生物発光を利用したバイオイメージング技術の開発 Invited
岩野 智
つくばがん研究会 2024.2.8
Event date： 2024.2.8
Language：Japanese Presentation type：Oral presentation (invited, special)
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生物発光を利用した脳機能の非侵襲イメージング Invited
岩野 智
第127回日本解剖学会総会 2022.3.28
Event date： 2022.3.27 - 2022.3.29
Language：Japanese Presentation type：Symposium, workshop panel (nominated)
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Development of Bioluminescence probe for non-invasive in vivo imaging in animal Invited
Satoshi Iwano
The 44th Annual Meeting of the Molecular Biology Society of Japan 2021.12.1
Event date： 2021.12.1 - 2021.12.3
Presentation type：Symposium, workshop panel (nominated)
Awards 【 display / non-display 】
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バイオインダストリー奨励賞
2020.10 Japan Bioindustry Association
Award type：International academic award (Japan or overseas)
Grant-in-Aid for Scientific Research 【 display / non-display 】
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生物発光バイオセンサーの開発基盤の構築と非侵襲イメージングへの応用
Grant number：25K00081 2025.02 - 2029.03
日本学術振興会 科学研究費助成事業 基盤研究(B)
岩野 智
Authorship：Principal investigator
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Development of Smart Nest
Grant number：24K03256 2024.04 - 2027.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Authorship：Coinvestigator(s)
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生物発光技術を活用した脳細胞内酸素化の可視化と脳保護のための循環管理最適化の探索
Grant number：23K18319 2023.06 - 2025.03
日本学術振興会 科学研究費助成事業 挑戦的研究(萌芽)
加藤 純悟(代表), 岩野 智, 寅丸 智子
Authorship：Principal investigator
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痛み恐怖記憶による痛み増幅機構の可視化と新規鎮痛標的の探索
Grant number：23H03004 2023.04 - 2027.03
日本学術振興会 科学研究費助成事業 基盤研究(B)
加藤 純悟(代表), 岩野 智, 寅丸 智子
Authorship：Principal investigator
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Elucidation of corticolimbic circuit mechanisms underlying psychosomatic correlation
Grant number：23H00398 2023.04 - 2027.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
Authorship：Coinvestigator(s)