|
Affiliation |
Engineering educational research section Department of Applied Chemistry Program |
|
Title |
Professor |
|
Related SDGs |
|
IZAWA Hironori
|
|
|
Research Areas 【 display / non-display 】
-
Nanotechnology/Materials / Bio chemistry
-
Nanotechnology/Materials / Polymer chemistry
-
Nanotechnology/Materials / Polymer materials
-
Nanotechnology/Materials / Functional solid state chemistry
Papers 【 display / non-display 】
-
Yanagi E., Akamatsu M., Suezawa T., Kaminaka H., Izawa H., Ifuku S.
Colloids and Surfaces A: Physicochemical and Engineering Aspects 700 2024.11
Publishing type：Research paper (scientific journal) Publisher：Colloids and Surfaces A: Physicochemical and Engineering Aspects
Partially deacetylated nanochitin (DAcNC) exhibits amphiphilicity owing to the hydrophilic protonated amino groups and hydrophobic surface planes of DAcNC. Although DAcNCs possess emulsifying abilities, their role and versatility as emulsifiers remain unclear. In this study, we investigated oil-in-water (O/W) type Pickering emulsions stabilized with DAcNCs. Stable emulsions were formed using a variety of oils such as vegetable oils, decane, and ether oils. Measurements of the interfacial tension and adsorption of DAcNC showed that formation of tolerant interfacial films stabilized the emulsions. Furthermore, the pH-triggered release of ibuprofen, an antipyretic drug, was demonstrated using the emulsion. Enhanced ibuprofen release was observed upon increasing the pH from 2.0 to 6.0. This phenomenon can be attributed to the deprotonation of ammonium moieties on the DAcNC, which induced the desorption of DAcNC from oil/water interfaces. The DAcNC emulsion can be useful for the oral administration of ibuprofen as an antipyretic agent, facilitating intestinal release.
-
Homogeneous microscopic bumps generated on λ-carrageenan cast film surface Reviewed
Izawa H., Toyoshima Y., Fujiwara N., Kawakami M., Tajima W., Ifuku S.
Colloids and Surfaces A: Physicochemical and Engineering Aspects 690 2024.6
Authorship：Lead author Publishing type：Research paper (scientific journal) Publisher：Colloids and Surfaces A: Physicochemical and Engineering Aspects
Here we investigate the phenomenon of bump formation on λ-carrageenan (CG). In this phenomenon, microscopic bumps (ca. 2.8 µm wide and ca. 0.4 µm high) form on the surface of λ-CG cast film created by evaporating a 1.0 wt% λ-CG solution in a PTFE petri dish at 60 ºC. We observe that the size of the bumps can be controlled by adjusting the concentration and volume of the λ-CG solution, and this is correlated with the film thickness. Specifically, as the film thickness increases, the size of the bumps also increases with strong correlation. We also investigate the mechanism underlying bump formation. We find that bumps form by complete drying and that the λ-CG film exhibits less-adhered inner layers with periodic curves resembling the bumps. These results suggest the curved layer structure is related to the formation mechanism. In other words, the drying shrinkage strain is relieved on each layer through curving, leading to the generation of bumps on the free surface. Therefore, we hypothesize that the fundamental mechanism behind the formation of these bumps lies in the self-organization of λ-CG, which results in the creation of an adhesion-less layered structure during the cast film formation process.
-
Melanin Upcycling: Creation of Polymeric Materials from Melanin Decomposition Products Reviewed
Morita T., Matsuura T., Izawa H., Kishikawa K., Kohri M.
ACS Sustainable Chemistry and Engineering 12 ( 18 ) 7115 - 7125 2024.5
Publishing type：Research paper (scientific journal) Publisher：ACS Sustainable Chemistry and Engineering
Melanin is a widely occurring biopolymer and has been the subject of much research, especially in dermatology. However, from a resource perspective, melanin is still an unutilized biomass because of its complex three-dimensional cross-linked structure, which makes it challenging to handle. Here, we demonstrate melanin upcycling by decomposing melanin and preparing polymeric materials from its products. A detailed study of the chemical decomposition products of artificial melanin, i.e., polydopamine, reveals that the melanin decomposition products are mainly oligomeric pyrrole derivatives containing carboxylic acids. Furthermore, decomposition experiments using natural melanin extracted from cuttlefish ink revealed that the composition of melanin decomposition products is almost identical regardless of the melanin source. We proposed a melanin decomposition mechanism and demonstrated the preparation of biobased polymer films and particles from melanin decomposition products. The use of melanin decomposition products as building blocks for material preparation is expected to lead to the development of new biodegradable polymers from biomass.
-
Khan N.F., Nakamura H., Izawa H., Ifuku S., Kadowaki D., Otagiri M., Anraku M.
Journal of Functional Biomaterials 14 ( 7 ) 2023.7
Publishing type：Research paper (scientific journal) Publisher：Journal of Functional Biomaterials
Arginine-rich membrane-permeable peptides (APPs) can be delivered to cells by forming complexes with various membrane-impermeable bioactive molecules such as proteins. We recently reported on the preparation of guanidinylated chitosan (GCS) that mimics arginine peptides, using chitosan, a naturally occurring cationic polysaccharide, and confirmed that it enhances protein permeability in an in vitro cell system. However, studies on the in vivo safety of GCS are not available. To address this, we evaluated the in vivo safety of GCS and its translocation into the gastrointestinal tract in rats after a single oral administration of an excessive dose (500 mg/kg) and observed changes in body weight, major organ weights, and organ tissue sections for periods of up to 2 weeks. The results indicated that GCS causes no deleterious effects. The results of an oral administration of rhodamine-labeled chitosan and an evaluation of its migration in the gastrointestinal tract suggested that the disappearance of rhodamine-labeled GCS from the body appeared to be slower than that of the non-dose group and pre-guanidinylated chitosan due to its mucoadhesive properties. In the future, we plan to investigate the use of GCS to improve absorption using Class III and IV drugs, which are poorly water-soluble as well as poorly membrane-permeable.
DOI： 10.3390/jfb14070340
-
Water-soluble guanidinylated chitosan: a candidate material for protein delivery systems Reviewed
Izawa H., Yagi A., Umemoto R., Ifuku S.
Polymer Journal 55 ( 8 ) 885 - 895 2023
Publishing type：Research paper (scientific journal) Publisher：Polymer Journal
Here, we introduce water-soluble guanidinylated chitosan (WGCS) as a candidate material for protein delivery systems to enhance the cellular internalization of protein/peptide drugs. A WGCS composed of 48.2% guanidinylated chitosan, 20.6% chitosan, and 31.2% chitin units was prepared with a low-molecular-weight chitosan (CS) lactate via a guanidinylation reaction with 1-amidinopyrazole hydrochloride. The Mn of WGCS was estimated by gel permeation chromatography analysis to be 7.6 × 103 (Mw /Mn = 1.5). The higher chitin content in WGCS than in common CS (<20%) is an important factor in achieving water solubility. WGCS showed ca. 2.5-fold higher internalization into HeLa cells than CS does. This clearly indicated that guanidinylation enhances internalization. In addition, endocytic pathways were suggested as a mechanism underlying internalization. Moreover, WGCS significantly enhanced the internalization of bovine serum albumin (BSA) in transport medium at pH 7.4 containing BSA: the internalized amount of BSA in the presence of WGCS was ca. 2-fold higher than in the presence of CS. This higher internalization was caused by efficient binding between WGCS and BSA via electrostatic interactions owing to the guanidino groups. Indeed, the affinity of the binding sites of WGCS is more than 10-fold higher than that of the binding sites of CS.
Presentations 【 display / non-display 】
-
酸性pHの制約を受けにくい高機能キトサン誘導体の開発
井澤浩則
令和6年度高分子学会九州支部講演会
Event date： 2024.12.6
Presentation type：Oral presentation (invited, special)
-
水溶性グアニジル化キトサン：置換度と分子量が溶解性に与える影響
荒木 美穂、井澤 浩則
2024年 繊維学会秋季研究発表会
Event date： 2024.11.28 - 2024.11.29
Presentation type：Oral presentation (general)
-
Solubility of Chitosan-Oligomers Having Different Degrees of Acetylation
Miho Araki, Hironori Izawa
International Symposium on Fiber Science and Technology
Event date： 2024.11.25 - 2024.11.28
Presentation type：Poster presentation
-
アセチル化度の異なるキトサンの溶解性とイオンコンプレックス形成挙動
荒木 美穂、井澤 浩則
第14回CSJ化学フェスタ２０２４
Event date： 2024.10.22 - 2024.10.24
Presentation type：Poster presentation
-
中性で均一分散するグアニジル化ナノキチンの開発
井澤 浩則、曽根 健聖、田嶋 航
第7３回高分子討論会
Event date： 2024.9.25 - 2024.9.27
Presentation type：Poster presentation
Grant-in-Aid for Scientific Research 【 display / non-display 】
-
多機能性キトサンを基盤とした抗酸化型機能食品と製剤特性改善素材に関する包括的研究
Grant number：24K09953 2024.04 - 2027.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
Authorship：Coinvestigator(s)
-
微細構造表面を誘起するスキン層の科学の開拓
Grant number：19K05616 2019.04 - 2022.03
日本学術振興会 科学研究費助成事業 基盤研究(C) 基盤研究(C)
井澤 浩則
Authorship：Principal investigator