NAGASHIMA Yoko

写真a

Affiliation

Faculty of Medicine School of Medicine Department of Anatomy, Ultrastructural Cell Biology

Title

Assistant Professor

Contact information

Contact information

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Papers 【 display / non-display 】

  • KMnO4/Pb staining allows uranium free imaging of tissue architectures in low vacuum scanning electron microscopy Reviewed

    澤口 朗, 北川 恭子, 長島 陽子, 高橋 伸育

    npj Imaging   2 ( 1 )   40   2024.10

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Scanning electron microscopy under low-vacuum conditions allows high-resolution imaging of complex cell/tissue architectures in nonconductive specimens. However, the conventional methods for metal staining of biological specimens require harmful uranium compounds, which hampers the applications of electron microscopy. Here, we introduce a uranium-free KMnO4/Pb metal staining protocol that allows multiscale imaging of extensive cell/tissue architectures to intensive subcellular ultrastructures. The obtained image contrast was equivalent to that of Ur/Pb staining and sufficient for ultrastructural observation, showing the fine processes of podocytes in the glomerulus, which were invisible by light microscopy. The stainability in the elastic tissue indicated that the distinct histochemical properties of KMnO4 oxidation led to Pb deposition and BSE signal enhancement superior to Ur staining. Elemental analysis clarified that the determinant of the backscattered electron signal intensity was the amount of Pb deposition enhanced by KMnO4 oxidation. This user-friendly method is anticipated to create a new approach for biomedical electron microscopy.

    CiNii Research

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Presentations 【 display / non-display 】

  • 過マンガン酸カリウム酸化電子染色法と低真空走査型電電顕解析の応用展開

    長島　陽子

    第67回　日本顕微鏡学会　九州支部集会・学術講演会  2025.12.13 

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    Event date： 2025.12.13

    Language：Japanese   Presentation type：Oral presentation (general)  

  • 過マンガン酸カリウム酸化電子染色法と低真空走査型電顕解析の応用展開

    長島　陽子

    日本顕微鏡学会第81回学術講演会  2025.6.10 

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    Event date： 2025.6.10

    Language：Japanese   Presentation type：Oral presentation (general)  

  • 卵巣癌発生に関わる卵管采の超微形態基盤研究 International conference

    長島　陽子

    第129回日本解剖学会総会・全国学術集会  2024.3.22 

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    Event date： 2024.3.21 - 2024.3.23

    Language：Japanese   Presentation type：Oral presentation (general)  

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