Affiliation |
Faculty of Medicine School of Medicine Department of Medical Sciences, Vascular and cellular dynamics |
Title |
Assistant Professor |
YAMAMOTO Kiyotake
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Research Areas 【 display / non-display 】
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Life Science / Pathological biochemistry
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Life Science / Cell biology
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Life Science / Pharmacology
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Life Science / Molecular biology
Papers 【 display / non-display 】
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Rap1 small GTPase is essential for maintaining pulmonary endothelial barrier function in mice. Reviewed International journal
Kiyotake Yamamoto, Haruko Watanabe-Takano, Eri Oguri-Nakamura, Hitomi Matsuno, Daiki Horikami, Tomohiro Ishii, Ryuji Ohashi, Yoshiaki Kubota, Koichi Nishiyama, Takahisa Murata, Naoki Mochizuki, Shigetomo Fukuhara
FASEB journal 37 ( 12 ) e23310 2023.12
Authorship:Lead author Language:English Publishing type:Research paper (scientific journal)
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Kiyotake Yamamoto, Yuki Takagi, Koji Ando, Shigetomo Fukuhara
Biological and Pharmaceutical Bulletin 44 ( 10 ) 1371 - 1379 2021.10
Authorship:Lead author Publishing type:Research paper (scientific journal) Publisher:Pharmaceutical Society of Japan
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Protocol for analysis of integrin-mediated cell adhesion of lateral plate mesoderm cells isolated from zebrafish embryos Reviewed
Seung-Sik Rho, Eri Oguri-Nakamura, Koji Ando, Kiyotake Yamamoto, Yuki Takagi, Shigetomo Fukuhara
STAR Protocols 2 ( 2 ) 100428 - 100428 2021.6
Publishing type:Research paper (scientific journal) Publisher:Elsevier BV
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Protein kinase C-δ signaling regulates glucagon secretion from pancreatic islets Reviewed
Yamamoto K., Mizuguchi H., Tokashiki N., Kobayashi M., Tamaki M., Sato Y., Fukui H., Yamasuchi A.
Journal of Medical Investigation 64 ( 1-2 ) 122 - 128 2017.1
Authorship:Lead author Language:English Publishing type:Research paper (scientific journal) Publisher:Journal of Medical Investigation
Accumulating evidence supports the “glucagonocentric hypothesis”, in which antecedent α-cell failure and inhibition of glucagon secretion are responsible for diabetes progression. Protein kinase C (PKC) is involved in glucagon secretion from α-cells, although which PKC isozyme is involved and the mechanism underlying this PKC-regulated glucagon secretion remains unknown. Here, the involvement of PKCδ in the onset and progression of diabetes was elucidated. Immunofluorescence studies revealed that PKCδ was expressed and activated in α-cells of STZ-induced diabetic model mice. Phorbol 12-myristate 13-acetate (PMA) stimulation significantly augmented glucagon secretion from isolated islets. Pre-treatment with quercetin and rottlerin, PKCδ signaling inhibitors, significantly suppressed the PMA-induced elevation of glucagon secretion. While Go6976, a Ca2+ -dependent PKC selective inhibitor did not suppress glucagon secretion. Quercetin suppressed PMA-induced phosphorylation of Tyr311 of PKCδ in isolated islets. However, quercetin itself had no effect on either glucagon secretion or glucagon mRNA expression. Our data suggest that PKCδ signaling inhibitors suppressed glucagon secretion. Elucidation of detailed signaling pathways causing PKCδ activation in the onset and progression of diabetes followed by the augmentation of glucagon secretion could lead to the identification of novel therapeutic target molecules and the development of novel therapeutic drugs for diabetes.
DOI: 10.2152/jmi.64.122
MISC 【 display / non-display 】
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石井 智裕, Yamamoto Kiyotake, 福原 茂朋
Inflammation & Immunology Vol.28 ( No.5 ) 360 - 364 2020.8
Language:Japanese Publishing type:Article, review, commentary, editorial, etc. (scientific journal)
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福原 茂朋, 盧 承湜, 小栗 エリ, Yamamoto Kiyotake, 石井 智裕
Science of the Living Body Vol.71 ( No.4 ) 359 - 363 2020.8
Language:Japanese Publishing type:Article, review, commentary, editorial, etc. (scientific journal)
Presentations 【 display / non-display 】
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Rap1低分子量Gタンパク質はVE-cadherin接着を増強することで肺の血管バリア機能を維持している
山本 清威, 渡邊-高野 晴子, 堀上 大貴, 石井 智裕, 久保田 義顕, 村田 幸久, 大橋 隆治, 望月 直樹, 福原 茂朋
第8回 血管生物医学会 若手研究会 2023.5.27
Event date: 2023.5.26 - 2023.5.27
Presentation type:Oral presentation (general)
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Rap1低分子量Gタンパク質による血管透過性制御とそれを標的とする血管透過性亢進がかかわる疾患の治療戦略
山本 清威, 渡邊-高野 晴子, 堀上 大貴, 髙木 夕希, 石井 智裕, 大橋 隆治, 久保田 義顕, 村田 幸久, 望月 直樹, 福原 茂朋
日本薬学会第142年会 2022.3.27
Event date: 2022.3.25 - 2022.3.28
Presentation type:Oral presentation (general)
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Rap1低分子量Gタンパク質は肺における血管透過性制御に必須である
山本 清威, 渡邊-高野 晴子, 堀上 大貴, 石井 智裕, 久保田 義顕, 村田 幸久, 大橋 隆治, 望月 直樹, 福原 茂朋
日本薬学会第142年会 2022.3.28
Event date: 2022.3.25 - 2022.3.28
Presentation type:Oral presentation (general)
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ヒト精子運動率関連遺伝子ERBB4をターゲットとした化合物の探索と精子運動率の検討
伊東 佑星, 小西 麻実, 山本 清威, 佐藤 陽一
第95回日本生化学大会 2022.11.10
Event date: 2022
Language:Japanese Presentation type:Poster presentation
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GWASによる精子濃度関連遺伝子座の同定とゲノム創薬研究
笠原 朱莉, 山本 清威, 佐藤 陽一
第95回日本生化学大会 2022.11.9
Event date: 2022
Language:Japanese Presentation type:Poster presentation
Grant-in-Aid for Scientific Research 【 display / non-display 】
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男性不妊症新規原因遺伝子の同定と治療法の開発研究
Grant number:22K09528 2022.04 - 2025.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)
Authorship:Coinvestigator(s)
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低分子量G蛋白質Rap1による血管透過性制御とその破綻によるARDSの病態解明
Grant number:21K15260 2021.04 - 2024.03
日本学術振興会 科学研究費助成事業 若手研究 若手研究
山本 清威
Authorship:Principal investigator