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Affiliation |
Engineering educational research section Department of Engineering Department of Applied Chemistry Program |
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Contact information |
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TAGAWA Satomi
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Papers 【 display / non-display 】
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Kojima Y., Sunagawa N., Tagawa S., Hatano T., Aoki M., Kurei T., Horikawa Y., Wada M., Funada R., Igarashi K., Yoshida M.
Carbohydrate Polymers 347 2025.1
Publishing type：Research paper (scientific journal) Publisher：Carbohydrate Polymers
Cellulose-binding domains (CBDs) play a vital role in cellulose degradation by enzymes. Despite the strong ability of brown-rot fungi to degrade cellulose in wood, they have been considered to lack or have a low number of enzymes with CBD. Here, we report the C-terminal domain of a lytic polysaccharide monooxygenase from the brown-rot fungus Gloeophyllum trabeum (GtLPMO9A-2) functions as a CBD, classified as a new family of carbohydrate-binding module, CBM104. The amino acid sequence of GtCBM104 shows no similarity to any known CBDs. A BLAST search identified 84 homologous sequences at the C-terminus of some CAZymes, mainly LPMO9, in basidiomycetous genomes. Binding experiments revealed GtCBM104 binds selectively to native crystalline cellulose (cellulose I), but not to artificially modified crystalline or amorphous cellulose, while the typical fungal CBD (CBM1) bound to all cellulosic materials tested. The adsorption efficiency of GtCBM104 to cellulose I was >20-times higher than that of CBM1. Adsorption tests and microscopic observations strongly suggested that GtCBM104 binds to the hydrophilic regions of cellulose microfibrils, while CBM1 recognizes the hydrophobic surface. The discovery of GtCBM104 strongly suggests that the contribution of CBD to the cellulose enzymatic degradation mechanism of brown-rot fungi is much larger than previously thought.
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Characterization of hybrid nanofibrils composed of xyloglucan and disintegrated bacterial cellulose Reviewed
Tagawa S., Tokuyasu K., Yamagishi K., Ike M., Amano Y., Mizuno M.
Cellulose 31 ( 4 ) 2239 - 2249 2024.3
Authorship：Lead author Publishing type：Research paper (scientific journal) Publisher：Cellulose
Nata puree (NP)—obtained by disintegrating nata de coco (bacterial cellulose [BC]) using a household blender—can be combined with tamarind seed gum (TG) to generate NPTG. In this study, BC fibrils (BC-TG) were prepared by removing free TG from NPTG and characterized. BC-TG exhibited high water dispersibility and relatively long nanofibrils (> 20 μm). We examined the distribution of xyloglucan, the main component of TG, on BC nanofibrils using immunofluorescence staining with calcofluor white, which stains the hydrophilic cellulose surface, and found that xyloglucan was adsorbed at different sites along the fibers. This indicated that BC-TG was a composite nanofibril of xyloglucan and BC. Furthermore, BC-TG showed a higher degree of adsorption on hydrophobic plastic substrates than BC did, suggesting a change in the surface properties of BC. Because the BC-TG preparation process is simple, requires only water and raw materials, and does not involve chemical reactions, it is expected to be an environmentally friendly method for the preparation and modification of BC nanofibrils.
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Kondo T., Ishikawa G., Kamogawa M., Tsujita Y., Yokota S., Tsuji T., Tagawa S., Tatsumi D.
ACS Applied Polymer Materials 6 ( 2 ) 1276 - 1285 2024.1
Publishing type：Research paper (scientific journal) Publisher：ACS Applied Polymer Materials
Cellulose nanofibrils (CNFs) have been employed as sustainable, eco-friendly fillers in high-performance nanocomposites, although such materials may exhibit poor impact tolerance. The present work synthesized high impact-resistance plastics based on plant cell-wall-like frameworks produced by coating 500 μm isotactic polypropylene (iPP) microspheres with ultratrace amounts of amphiphilic bamboo CNFs prepared by the aqueous counter collision method. These microspheres were spontaneously coated when combined with a suspension containing 0.03 wt %/iPP of the CNFs by vibrational mixing under ambient conditions. A simple injection molding process was used to induce the fusion of the coated iPP microspheres and generate a three-dimensional framework of CNFs resembling a plant cell wall structure. This framework with ultralow amounts of amphiphilic CNFs was found to improve the impact shock resistance of the plastic. The facile, eco-friendly process reported herein could be used to facilitate the upcycling of plastics and thus promote the sustainable use of resources.
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Immobilization of oligo DNA strands on TEMPO-oxidized pulp fibers and evaluation of duplex formation Reviewed
Nakauchi H., Tagawa S., Mizuno M., Amano Y.
Cellulose 31 ( 2 ) 857 - 868 2024.1
Publishing type：Research paper (scientific journal) Publisher：Cellulose
Paper fiber functionalization by modification with DNA molecules expands the applicability and specificity of paper-substrate analytical devices such as microfluidic paper-based analytical devices (µPADs). In this study, a simple amide condensation reaction was performed on 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized pulp fibers to covalently immobilize oligo DNA chains. The fluorescently labeled DNA with confocal laser scanning microscopy, revealed DNA immobilization on the pulp fiber surface and inside the pulp fiber wall. This immobilized DNA sequence-selectively recognized the complementary strand at concentrations of 10−8 M and above. This DNA duplex formation was also explored in terms of the negative charges on the pulp surface. With greater concentrations of carboxyl groups on the pulp material, DNA duplex formation efficiency was lowered. This is proposed to be due to the negatively charged surface imposing a repulsive electrostatic interaction with the negatively charged DNA. In addition, duplex formation and melting on the pulp surface was shown to be temperature-dependent and reversible. Graphical abstract: [Figure not available: see fulltext.]
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Preparation of Mycelium Pulp from Mushroom Fruiting Bodies Reviewed
Nakauchi H., Amano Y., Tagawa S.
ACS Sustainable Chemistry and Engineering 11 ( 44 ) 15789 - 15794 2023.11
Authorship：Corresponding author Publishing type：Research paper (scientific journal) Publisher：ACS Sustainable Chemistry and Engineering
We developed a new method to extract mycelial fibers, without destroying their structure, from the fruiting bodies of mushrooms. After chemical treatment with NaOH and H2O2, the fruiting bodies were decolorized via an environmentally friendly method using sunlight irradiation. The visible light reflectance of decolorized fruiting bodies was more than 80%. Ultrasonic treatment was used to defibrillate the fruiting bodies at the mycelial level, and a white micrometer-sized dispersion of mycelial fibers (mycelium pulp) was obtained. The mycelium retained its structure, demonstrating a thick linear mycelium pulp (width: 8.0 ± 3.4 μm) in Flammulina velutipes and a thin branched mycelium pulp (width: 2.3 ± 0.6 μm) in Ganoderma lucidum. The mycelium pulp is a completely new material that maintains its mycelial structure, unlike previously reported materials derived from fruiting bodies. The mycelium pulp demonstrates excellent deformability and can be used to create one- to three-dimensional deformable products, showing a wide range of material applicability.
MISC 【 display / non-display 】
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ナノセルロースにおける蛍光顕微鏡を用いた可視化技術の応用
田川聡美
応用糖質科学 13 ( 4 ) 2023
Authorship：Lead author, Corresponding author Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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蛍光顕微鏡を用いたセルロースナノフィブリルの繊維長測定
田川聡美
Cellulose Communications 28 ( 3 ) 2021
Authorship：Lead author, Corresponding author Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
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シラカバ培養細胞由来プロトプラストの環境ストレスに応答した細胞壁形成挙動
田川聡美
木科学情報 24 ( 3 ) 2017
Authorship：Lead author, Corresponding author Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
Grant-in-Aid for Scientific Research 【 display / non-display 】
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きのこ細胞壁中に存在する多糖間結合の解明
Grant number：24K01821 2024.04 - 2029.03
日本学術振興会 科学研究費助成事業 基盤研究(B)
Authorship：Coinvestigator(s)
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ハイドロフォビンの局在性の微視的解析から迫る担子菌の菌糸体形成機構
Grant number：23K13992 2023.04 - 2027.03
日本学術振興会 科学研究費助成事業 若手研究
Authorship：Principal investigator
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木質バイオマスの酵素糖化の効率化の鍵を握るヘミセルロース分解酵素の特異性の解明
Grant number：22K05762 2022.04 - 2025.03
日本学術振興会 科学研究費助成事業 基盤研究(C)
Authorship：Coinvestigator(s)