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Affiliation |
Faculty of Agriculture Region of Animal and Plant Biosciences |
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Assistant Professor |
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Related SDGs |
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FUJIWARA Yosuke
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Degree 【 display / non-display 】
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Agriculture ( 2026.3 Kyoto University )
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Agriculture ( 2023.3 Kyoto University )
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Agriculture ( 2021.3 Kyoto University )
Research Areas 【 display / non-display 】
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Environmental Science/Agriculture Science / Horticultural science / 果樹園芸学
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Life Science / Plant molecular biology and physiology
Papers 【 display / non-display 】
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Fujiwara Y., Nishiyama S., Kusumi A., Okamoto-Furuta K., Yamane H., Yonemori K., Tao R.
Plant Physiology 200 ( 1 ) 2026.1
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Plant Physiology
<jats:p>Spatial gene expression and 2D/3D ultrastructural analyses reveal a unique intercellular sequestration mechanism underlying massive proanthocyanidin accumulation in persimmon fruit.</jats:p>
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Fujiwara Y., Nishiyama S., Onoue N., Kono A., Sato A., Yonemori K., Tao R.
Horticulture Journal 94 ( 2 ) 148 - 161 2025
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Horticulture Journal
Persimmon fruit synthesizes and accumulates proanthocyanidins (PAs), resulting in its astringent taste. The pollination-constant and non-astringent (PCNA) trait is conferred by recessive alleles at the ASTRINGENCY (AST) locus, which has been exploited to control PA accumulation of persimmon fruit. However, some individuals with recessive homozygosity at the AST marker locus in breeding populations produce mature fruit with astringency, thereby reducing the marker-selection efficiency for the PCNA trait. In this study, we genetically and physiologically characterized the predicted ast-homozygous individuals exhibiting strong astringency in a cross population of ‘Taigetsu’ (non-PCNA) and ‘Kanshu’ (PCNA). The fruit PA content of these astringent individuals was higher than that of the other PCNA fruit, but lower than that of the nonPCNA fruit. These astringent individuals were similar to the other PCNA plants in terms of tannin cell size. Gene expression analysis revealed that PA synthesis-related genes (e.g., DkMYB4, DkMYC1, and DkANR) were expressed at a higher level in the astringent individuals than in the other PCNA fruit during the early fruit development stage. Furthermore, in the presumed ast-homozygous individuals with various PA contents, the expression of cellular function-related genes was highly correlated with PA accumulation. These genes may be important for tannin cell development and PA synthesis, potentially offering a novel approach to further modulate astringency in persimmons.
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Efficient Transient Expression for Functional Analysis in Fruit Using the Tsukuba System Vector
Omori Masafumi, Fujiwara Yosuke, Yamane Hisayo, Miura Kenji, Tao Ryutaro
The Horticulture Journal 92 ( 3 ) 261 - 268 2023
Language：English Publishing type：Research paper (scientific journal) Publisher：The Japanese Society for Horticultural Science
Evaluating the function of genes expressed in fruit tissues of fruit tree species using a genetic transformation approach is a long process because the trees are generally recalcitrant to genetic transformation and cannot bear fruit during their long juvenile phases. Transient gene expression in fruit enables the functional analysis of genes associated with fruit traits, which may accelerate the study of fruit physiology. Here, by using the recently developed “Tsukuba system”, we successfully established an efficient transient expression system in harvested fruit tissues. The “Tsukuba system” utilizes a combination of the geminiviral replication system and a double terminator, which ensures sufficient levels of transgene expression. We used blueberry fruit as a model to characterize the applicability of this system for transient expression in fruit tissue. The pTKB3-EGFP vector was introduced by agroinfiltration into the fruit tissues of several blueberry cultivars. We found that transient GFP fluorescence in fruit peaked 4–6 days after agroinfiltration. <i>Agrobacterium</i> suspensions were easily injected into soft, mature fruit, and GFP was strongly expressed; however, hard, immature fruit were not penetrable by <i>Agrobacterium</i> suspensions, and GFP was rarely detected. We then tested the applicability of the developed system to other fruit tree species: six families, 17 species, and 26 cultivars. GFP fluorescence was detected in all species, except for Japanese apricot. In blueberry, bilberry, sweet cherry, apricot, and satsuma mandarin, GFP was highly expressed and observed in a large proportion of the flesh. In kiwifruit, hardy kiwifruits, persimmon, peach, apple, European pear, and grape, GFP fluorescence was limited to certain parts of the fruits. Finally, transient <i>VcMYBA1</i> overexpression in blueberry was tested as a model for gene functional analysis in fruit. Transient <i>VcMYBA1</i> overexpression induced red pigmentation in the flesh, suggesting that <i>VcMYBA1</i> expression caused anthocyanin accumulation. This study provides a technical basis for the rapid evaluation of genes expressed in fruit, which will be useful for gene function evaluation studies in fruit crops with long juvenile phases.
DOI： 10.2503/hortj.QH-062
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Fujiwara Y., Nishiyama S., Onoue N., Matsuzaki R., Yonemori K., Tao R.
Acta Horticulturae 1338 ( 1338 ) 269 - 275 2022.4
Authorship：Lead author Publishing type：Research paper (scientific journal) Publisher：Acta Horticulturae
The astringent taste caused by proanthocyanidins (PAs), which still remains at fruit maturation or harvesting stage, is a unique characteristic of persimmon. Pollination-constant and non-astringent (PCNA) type cultivars stop accumulating PAs in the early stage of fruit development, allowing the fruit to be edible without artificial de-astringency treatment. The PCNA trait is conferred by a recessive allele at a single locus, ASTRINGENCY (AST). The AST-locating genomic region (AST region) has been delimited in the polyploid D. kaki genome by comparative mapping, but the AST gene has yet to be identified. Here, we characterized a genomic region of diploid D. oleifera that corresponds to the AST region in D. kaki. Firstly, we obtained mRNA-seq data from siblings from a cross between 'Taiten' (non-PCNA) and 'Kanshu' (PCNA) and their parents at two fruit developmental stages in 2015 and at five stages in 2016. The genetic analysis concluded that there is no nonsynonymous substitution in the genic regions in the AST region. Differential expression analysis between PCNA and non-PCNA detected two differentially expressed genes (FDR <0.05) in the region, whereas their expression patterns were not correlated with PA accumulation during fruit development. We also sequenced small RNA from the same population as the differential expression analysis. Small RNAs were annotated in the D. oleifera genome, and 6 small RNAs in the AST region with significantly different normalized counts (p<0.05) between PCNA and non-PCNA were identified. These small RNAs were found to target 5 genes, whereas there were no effects in their mRNA expression levels. In conclusion, this study identified none of the promising AST candidates with clear difference between the astringent and non-astringent alleles. More detail information of polyploid D. kaki genome is required to obtain comprehensive set of candidate genes.
MISC 【 display / non-display 】
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Comparative analysis of fruit metabolites between astringent and non-astringent persimmons Reviewed
浅草遼太郎, 藤原陽介, 西山総一郎, 尾上典之, 河野淳, 山根久代, 田尾龍太郎
園芸学研究 別冊 24 ( 2 ) 2025
Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
Presentations 【 display / non-display 】
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Identification of a single candidate gene responsible for fruit astringency type in hexaploid persimmon through a forward-genetic approach
Yosuke Fujiwara, Soichiro Nishiyama, Noriyuki Onoue, Ryusuke Matsuzaki, Hisayo Yamane, Keizo Yonemori, Ryutaro Tao
International Horticulture Conference (IHC) 2026 2026.8
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カキのastホモ接合個体 における渋味果のプロアントシアニジン分析
藤原陽介, 西山総一郎, 浅草遼太郎, 尾上典之, 松﨑隆介, 山根久代, 宮下正弘, 田尾龍太郎
園芸学会令和八年度春季大会 2026.3
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完全甘ガキ形質決定遺伝子候補の機能解析
藤原陽介, 西山総一郎, 山根久代, 田尾龍太郎
園芸学会令和七年度秋季大会 2025.9
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プロアントシアニジン蓄積制御遺伝子の迅速な機能解析に向けたモデル評価系の予備検討
藤原陽介, 浅草遼太郎, 西山総一郎, 田尾龍太郎
園芸学会令和七年度春季大会 2025.3
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Haplotype-Phased Assembly and Polymorphism Analysis of the Delimited ASTRINGENCY Locus in Hexaploid Persimmon
Fujiwara, Y, S. Nishiyama, R. Matsuzaki, N. Onoue, R. Tao
Plant and Animal Genome Conference / PAG32 2025.1
Awards 【 display / non-display 】
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Young Minds Award for best oral presentation in IHC2026
2026.8 International Society of Horticulture Science
Yosuke Fujiwara, Soichiro Nishiyama, Noriyuki Onoue, Ryusuke Matsuzaki, Hisayo Yamane, Keizo Yonemori, Ryutaro Tao
Award type：International academic award (Japan or overseas)
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優秀発表賞 口頭発表部門
2025.9 園芸学会令和七年度秋季大会
藤原陽介・西山総一郎・山根久代・田尾龍太郎
Award type：International academic award (Japan or overseas)
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優秀発表賞 ポスター発表部門
2024.11 園芸学会令和六年度秋季大会
藤原陽介・西山総一郎・尾上典之・松﨑隆介・河野淳・佐藤明彦・米森敬三・田尾龍太郎
Award type：International academic award (Japan or overseas)
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AHC2023 Young Minds Award for best oral presentation
2023.8 IV Asian Horticultural Congress
Yosuke Fujiwara, Soichiro Nishiyama, Noriyuki Onoue, Keizo Yonemori, Ryutaro Tao
Award type：International academic award (Japan or overseas)
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ISHS Young Minds Award for best poster presentation
2021.9 7th International Symposium on Persimmon
Yosuke Fujiwara, Soichiro Nishiyama, Noriyuki Onoue, Ryusuke Matsuzaki, Keizo Yonemori, Ryutaro Tao
Award type：International academic award (Japan or overseas)
Grant-in-Aid for Scientific Research 【 display / non-display 】
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Studies on molecular mechanism of proanthocyanidin accumulation in persimmon fruits
Grant number：23KJ1313 2023.04 - 2026.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows
Authorship：Principal investigator
Available Technology 【 display / non-display 】
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カンキツ類のゲノム編集に関する研究
カキの完全甘ガキ性に関する研究Home Page： 宮崎大学果樹園芸学研究室HP