YAMAGUCHI Ryoji

写真a

Title

Professor

Date of Birth

1955

Laboratory Address

1-1 Gakuenkibanadai-Nishi, Miyazaki

Research Fields, Keywords

Life science

Mail Address

E-mail address

Laboratory Phone number

+81-985-58-7271

Laboratory Fax number

+81-985-58-7271

Graduating School 【 display / non-display

  •  
    -
    1978.03

    University of Miyazaki   Faculty of Agriculture   Veterinary Science   Graduated

Graduate School 【 display / non-display

  •  
    -
    1980.03

    The University of Tokyo  Graduate School, Division of Agricultural Science  Master's Course  Completed

  •  
    -
    1981.03

    The University of Tokyo  Graduate School, Division of Agricultural Science  Doctor's Course  Unfinished Course

Field of expertise (Grants-in-aid for Scientific Research classification) 【 display / non-display

  • Veterinary medical science

  • Veterinary medical science

Qualification acquired 【 display / non-display

  • JCVP

 

Research Career 【 display / non-display

  • Reasearch on Malignant Animal Infectious Disease

    International Joint Research Projects  

    Project Year: 2010.04  -   

  • Investigatiuon of viral infection in pigs

    The Other Research Programs  

    Project Year: 2009.01  -   

  • Studies on Canine distemper virus(CDV)

    (not selected)  

    Project Year: 2004.04  -   

Papers 【 display / non-display

  • Local extensive granulomatous inflammation of the neck region and lymphangitis caused by Lichtheimia corymbifera infection in a Japanese Black calf

    Teh A., Hirai T., Ito S., Hidaka Y., Goto Y., Furukawa H., Sawada J., Yamaguchi R.

    Medical Mycology Case Reports   21   37 - 40   2018.09  [Refereed]

    Joint Work

     View Summary

    © 2018 The Authors A 7-month-old female Japanese Black calf developed elongated, nodular mass measuring 30 × 16 cm extended from the retropharyngeal region to mid lateral neck region. Histological examination revealed granulomatous lymphangitis with non-septate fungal hyphae recognized throughout the lesions. Fungal culture, DNA sequencing and molecular phylogenetic tree analysis confirmed the sequence of Lichtheimia corymbifera. The lymphogenous route was speculated to be the main route of fungal spread leading to the characteristic nodular appearance of this case.

    DOI

  • Overexpression of a virus-like particle influenza vaccine in Eri silkworm pupae, using Autographa californica nuclear polyhedrosis virus and host-range expansion.

    Maegawa K, Shibata T, Yamaguchi R, Hiroike K, Izzati UZ, Kuroda K, Sugita S, Kawasaki K, Nerome R, Nerome K

    Archives of virology     2018.07  [Refereed]

    Joint Work

    DOI PubMed

  • Development of a Japanese encephalitis virus genotype V virus-like particle vaccine in silkworms.

    Nerome K, Yamaguchi R, Fuke N, Izzati UZ, Maegawa K, Sugita S, Kawasaki K, Kuroda K, Nerome R

    The Journal of general virology   99   897 - 907   2018.07  [Refereed]

    Joint Work

    DOI PubMed

  • Characterization of anti-Müllerian hormone in a case of bovine male pseudohermaphroditism

    Kitahara G., El-Sheikh Ali H., Teh A., Hidaka Y., Haneda S., Mido S., Yamaguchi R., Osawa T.

    Reproduction in Domestic Animals   53   809 - 813   2018.06  [Refereed]

    Joint Work

     View Summary

    © 2018 Blackwell Verlag GmbH The current report aimed to characterize plasma anti-Müllerian hormone (AMH) in bovine male pseudohermaphroditism. The blood AMH concentration in a Japanese Black male pseudohermaphrodite calf was compared with pre- and post-pubertal male and female calves and castrated calves. The concentration in the case was higher than in post-pubertal males, castrated males, and pre- and post-pubertal female calves (p <.05), but similar to that in pre-pubertal male calves. After extraction of the testes, the concentration in the case dropped to a certain extent. The extracted testes expressed AMH, as detected by immunohistochemistry. This study is the first to show the characterization of AMH in a male pseudohermaphrodite calf. AMH levels in peripheral blood might be useful to diagnose male pseudohermaphroditism in cattle.

    DOI

  • Histological and immunohistochemical evaluation of granulosa cells during different stages of folliculogenesis in bovine ovaries

    Teh A., Izzati U., Mori K., Fuke N., Hirai T., Kitahara G., Yamaguchi R.

    Reproduction in Domestic Animals   53   569 - 581   2018.06  [Refereed]

    Joint Work

     View Summary

    © 2018 Blackwell Verlag GmbH Bovine granulosa cells (GC) vary in their morphological aspect during different stages of folliculogenesis. In this study, 10 morphologically normal bovine ovaries were collected to study the structural aspects of different stages of GC using intermediate filament protein antibodies including cytokeratin AE1/AE3 (AE1/AE3), vimentin, nectin-4 and desmin. Hormonal immunolocalization was assessed using the immunomarkers anti-Müllerian hormone (AMH) and inhibin alpha. In addition, tumour markers and proliferation markers using c-erbB-2 oncoprotein and proliferating cell nuclear antigen, respectively, were investigated. The immunolabelling of AE1/AE3 in GC was strongest in the early follicle stage and gradually decreased when reaching the Graafian follicle stage. Its immunolabelling increased again as the stage progressed from stage I to stage III. The immunolabelling of inhibin alpha was inversely proportional to that of AE1/AE3 in the developing ovarian follicles as their immunolabelling is opposite to each other during folliculogenesis. AMH was immunopositive in almost all GC stages in different intensities and percentages, except for some negative staining in the atretic IV follicles. The atretic IV follicle is a unique type of atretic follicle that shows Call-Exner body formation, which was mainly found in older cows in this study. The distinct patterns of immunoreactivity for various types of immunomarkers in the different GC stages will play an important role in diagnostic assistance of various follicle conditions, including cystic ovaries and GC tumours.

    DOI

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Books 【 display / non-display

  • Color Atlas of aAnimal Pathology, 2nd Edition

    (Part: Contributor )

    2018.01 ISBN: 978-4-83---3268-4

  • Systemic Animal Pathology, 2nd Edition

    (Part: Joint Work )

    2015.03 ISBN: 978-4-8300-3225-7

  • Systemic Animal Pathology, 2nd Edition

    (Part: Joint Work )

    2010.02 ISBN: 978-4-8300-3225-7

Other research activities 【 display / non-display

  • Daiagnostic Pathology

    Fieldwork 

    1990.10
     
     

Presentations 【 display / non-display

  • A PRIMITIVE NEUROECTODERMAL TUMOR

    Van Diep, Naoyuki Fuke, Angeline Teh Ping Ping, Uda Zahli Nurul Izzati, Takuya Hirai, Ryoji Yamaguchi

    JCVP学術集会  2017.03  -  2017.03  JCVP

  • 牛の顆粒膜細胞腫23例の多彩な増殖パターンと免疫組織化学的解析による発生起源解析

    Teh Angeline Ping Ping1, 北原 豪2, 伊藤 宗磨1, 平井 卓哉1, 山口 良二1

    JCVP学術集会  2017.03  -  2017.03  JCVP

  • PATHOLOGICAL CHARACTERISATION OF THE PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME OUTBREAK IN NORTHERN VIETNAM

    Yamaguchi Ryoji1, Nguyen Thi Huong Giang 1,, Lan Thi Nguyen2, Nguyen Huu Nam2, Hirai Takuya1  [Invited]

    Solutions to Emerging and Zoonotic Diseases  2016.10  -  2016.10  Vietnam National University of Agriculture

     View Summary

    In 2007, a highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) was emerged in Vietnam and spread to nearly all regions of Vietnam by 2010. Ten representative pigs of different age groups naturally infected with HP-PRRSV in northern Vietnam in 2010 were used to characterise the pathological features after viral detection was confirmed using reverse transcription polymerase chain reaction (RT-PCR) and viral isolation. The clinical signs and gross findings in these pigs included high fever (>40.2°C), red skin, blue ears, anorexia, respiratory distress, diarrhoea, haemorrhagic pleuritis and lymphadenopathy. Reproductive failure was the main clinical feature in sows. PRRSV infection-associated histopathological lung and lymph node lesions were observed frequently, regardless of the age. Lung lesions were characterised by interstitial pneumonia and were occasionally associated with haemorrhage and fluid exudation following alveolar collapse. Lymph nodes exhibited characteristic haemorrhage and apoptosis, lymphocytic depletion and disorganisation secondary to fibrosis and capillary formation. Haematoxylin–eosin staining or caspase-3 immunohistochemistry revealed apoptosis induction in various tissues and organs, particularly the lymph nodes and lungs. Primarily haemorrhagic microscopic lesions were commonly observed in other organs (such as spleen, liver, heart and kidney). Immunohistochemical examination revealed the distribution of HP-PRRS antigen in the lung, lymph node, liver and kidney macrophages and lung and kidney epithelial cells. Therefore, pigs infected naturally with HP-PRRS in the field likely suffer from multitissue tropism and massive apoptotic cellular death, leading to the appearance of disease.

  • PATHOLOGICAL CHARACTERIZATION OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME OUTBREAK IN NORTHERN VIETNAM

    Ryoji Yamaguchi、 Huong Giang Thi Nguyen1, Lan Thi Nguyen2, Nguyen Huu Nam2, Hirai Takuya

    2015年アジア獣医病理学会ASVP  2015.11  -  2015.11  アジア獣医病理学会

  • Sequence analysis and molecular epidemiology of the reemerging porcine epidemic diarrhea virus strains including some novel strains with large deletion in the spike gene isolated in Japan

    Nguyen Van Diep, Masuo Sueyoshi, Nguyen Thi Lan, Takuya Hirai, Yamaguchi Ryo

    第7回国際新興・再興感染症豚病学会  2015.06  -  2015.06  The 7th International Symposium on Emerging and Re-emerging Pig Diseases 2015 (ISERPD2015 in Kyoto)

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