Papers - SAKAI Go
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Highly selective and sensitive SPR immunosensor for detection of methamphetamine
Sakai G., Nakata S., Uda T., Miura N., Yamazoe N.
Electrochimica Acta 44 ( 21 ) 3849 - 3854 1999.6
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Electrochimica Acta
Highly sensitive and selective detection of methamphetamine (MA) was realized by using an immunosensor based on surface-plasmon-resonance (SPR) phenomena. A methamphetamine-bovine serum albumin (MA-BSA) conjugate was prepared as an antigen. It was immobilized on the Au thin film of sensor chip by physical adsorption. The incident angle of the MA-BSA immobilized sensor increased with increasing concentration of anti-MA-BSA antibody up to ca. 20 μg/ml (ppm) and then almost saturated above this concentration. The addition of MA into the antibody solution (20 ppm) was found to decrease the incident angle shift sharply because of the inhibition effect of MA. The present sensor was confirmed to detect MA very sensitively in the concentration range of 0.1-1000 ng/ml (ppb). Moreover, it was found that the sensor could detect MA in the PBS solution containing 1% human urine without any significant changes in incident angle shift due to nonspecific adsorption. Furthermore, it was confirmed that the interference effect of methylephedrine on the MA sensitivity of the sensor was fairly small. From the correlation between incident angle shift and MA concentration, the affinity constants of anti-MA antibody to MA (in liquid media) and MA-BSA (immobilized) were evaluated by assuming Langmuir isotherm and equilibrium state of immunoreaction.
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Highly selective and sensitive SPR immunosensor for detection of methamphetamine
共著者：G. Sakai, S. Nakata, T. Uda, N.Miura, N. Yamazoe
Electrochimica Acta, Vol. 44/21-22, 3849-3854 1999.6
Language：English Publishing type：Research paper (scientific journal)
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Nakata S., Sakai G., Uda T., Miura N., Yamazoe N.
IEEJ Transactions on Sensors and Micromachines 119 ( 11 ) 581 - 586 1999
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：IEEJ Transactions on Sensors and Micromachines
Highly sensitive detection of methamphetamine (MA) was realized by using an immunosensor based on surface plasmon resonance (SPR) phenomena. A methamphetamine-bovine serum albumin (MA-BSA) conjugate was prepared as an antigen, and it was immobilized on the Au thin film of sensor-chip. Introduction of MA into the antibody solution was found to decrease the incident angle shift sharply because of the inhibition effect of MA. Association constants between anti-MA antibody and MA as well as anti-MA antibody and MA-BSA were evaluated from the Langmuir isotherm adsorption model. The association constants between anti-MA antibody and MA or MA-BSA were estimated as 5.68×106M-1, 2.92×107 M-1 respectively. The association rate constant and dissociation rate constant were also estimated from typical transient responses to antibody solutions. © 1999, The Institute of Electrical Engineers of Japan. All rights reserved.
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Chung W., Sakai G., Shimanoe K., Miura N., Lee D., Yamazoe N.
Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers 37 ( 9 A ) 4994 - 4998 1998.9
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
Thin films of indium oxide were prepared on a silicon substrate by a spin-coating method using a coating solution dissolving In(OH)3, acetic acid and ammonium carboxymethyl cellulose. The films consisted of a dense stack of fairly uniform grains, adhering well to the substrate, as observed with atomic force microscopy (AFM) and scanning electron microscopy (SEM). The film thickness was well controlled by repeating the spin-coating. The sensing properties of the In2O3 film to CO, H2 and C3H8 depended on the film thickness and temperature, fairly good sensing performance to CO being achieved with a 140-nm-thick film at 350°C. The contact between platinum electrode and In2O3 in these devices was found to be slightly non-Ohmic, unlike that in the sintered block type device.
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酸化ビスマスを用いた半導体NOセンサ
共著者：酒井 剛、神川晃幸、三浦則雄、山添 曻
電気学会E部門誌、センサ・マイクロマシン部門誌、Vol. 118-E, 578-583頁 118-E 578 - 583 1998.9
Language：Japanese Publishing type：Research paper (scientific journal)
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A surface plasmon resonance-based immunosensor for highly sensitive detection of morphine
Sakai G., Ogata K., Uda T., Miura N., Yamazoe N.
Sensors and Actuators, B: Chemical 49 ( 1-2 ) 5 - 12 1998.6
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Sensors and Actuators, B: Chemical
Highly sensitive and selective detection of morphine (MO) based on surface-plasmon-resonance (SPR) was realized by using an anti-MO monoclonal antibody and MO-bovine serum albumin (MO-BSA) conjugate (antigen). MO-BSA was immobilized on the Au thin film of the SPR sensor chip by physical adsorption. The incident angle of the SPR system using the MO-BSA immobilized chip increased almost linearly with increasing concentration of antibody up to ca. 5 μg ml-1(ppm). The addition of MO to the antibody solution (5 ppm) was found to reduce the incident angle shift sharply because of the inhibition effect of MO. Based on this inhibiting principle, the present sensor could detect MO in the concentration range 0.1-10 ng ml-1(ppb). The affinity constants between the antibody and the antigens (MO and MO-BSA) could be obtained by assuming a Langmuir adsorption model for the immunoreaction system. © 1998 Elsevier Science S.A. All rights reserved.
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Gas sensing properties of indium oxide thin film on silicon substrate prepared by spin-coating method Reviewed
共著者：W.-Y. Chung, G. Sakai, K. Shimanoe, N. Miura, D.-D. Lee, N. Yamazoe
Japanease Journal of Applied Physics, Vol. 37, 4994-4998 37 4994 - 4998 1998.6
Language：English Publishing type：Research paper (scientific journal)
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Preparation of indium oxide thin film by spin-coating method and its gas-sensing properties
Chung W., Sakai G., Shimanoe K., Miura N., Lee D., Yamazoe N.
Sensors and Actuators, B: Chemical 46 ( 2-3 ) 139 - 145 1998.2
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Sensors and Actuators, B: Chemical
Thin films of In2O3were fabricated on an alumina substrate by spin-coating of an aqueous solution prepared from In(OH)3, acetic acid and ammonium carboxymethyl cellulose, followed by drying at 110°C and calcining at 600°C. As observed by SEM, the films consisted of a dense stack of tiny In2O3particles (23-27 nm in diameter) and covered large grains of the alumina substrate well. Subsequently, film thickness was well controlled by the number of spin-coatings. Gas sensing properties were strongly dependent on the film thickness and temperature and fairly excellent sensing performances to H2, CO and C3H8were obtained with a 160 nm-thick film (5 times coated) at 350°C. © 1998 Elsevier Science S.A. All rights reserved.
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Surface Plasmon Resonance-Based Immunosensor for Highly Sensitive Detection of Morphine Reviewed
共著者：G. Sakai, K. Ogata, T. Uda, N. Miura, N. Yamazoe
Sensors and Actuators B,Vol. 49/1-2, 5-12 49 ( 1-2 ) 5 - 12 1998.2
Language：English Publishing type：Research paper (scientific journal)
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Preparation of indium oxide thin film by spin-coating method and its gas-sensing properties Reviewed
共著者：W.-Y. Chung, G. Sakai, K. Shimanoe, N. Miura, D.-D. Lee, N. Yamazoe
Sensors and Actuators B, Vol. 46/2, 139-145 46 ( 2 ) 139 - 145 1998.2
Language：English Publishing type：Research paper (scientific journal)
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Preparation of indium oxide thin film by spin-coating method and its gas-sensing properties
Chung W., Sakai G., Shimanoe K., Miura N., Lee D., Yamazoe N.
Sensors and Actuators, B: Chemical B46 ( 2 ) 139 - 145 1998.1
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Sensors and Actuators, B: Chemical
Thin film of In2O3were fabricated on an alumina substrate by spin-coating of an aqueous solution prepared from In(OH)3, acetic acid and ammonium carboxymethyl cellulose, followed by drying at 110 °C and calcining at 600 °C. As observed by SEM, the films consisted of a dense stack of tiny In2O3particles (23-27 nm in diameter) and covered large grains of the alumina substrate well. Subsequently, film thickness was well controlled by the number of spin-coatings. Gas sensing properties were strongly dependent on the film thickness and temperature and fairly excellent sensing performances to H2, CO and C3H8were obtained with a 160 nm-thick film (5 times coated) at 350 °C.
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Bismuth Oxide-Based Semiconductor Gas Sensor for Nitrogen Monoxide
Sakai G., Jinkawa T., Miura N., Yamazoe N.
IEEJ Transactions on Sensors and Micromachines 118 ( 12 ) 578 - 583 1998
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：IEEJ Transactions on Sensors and Micromachines
A search for semiconducting oxides was carried out to design a sensor selective to NO over NO2. Bi2O3was very selective to NO though not very sensitive among the various oxides examined, and NiO was proved to be the most effective promoter to Bi2O3for enhancing NO sensitivity. The sensor element using 5 wt%NiO-Bi2O3showed fairly good sensing properties to NO in the range of 0-400 ppm in sensitivity, selectivity over NO2and response rate, at 300°C. The cross-sensitivities to C3H6, C3H8, H2and O2were modest or insignificant, compared to the sensitivity to NO. Catalytic activity tests and TPD measurements indicated that the response to NO (increase in resistance) was associated with its reaction with the surface oxygen of the element, while that to NO2(decrease in resistance) resulted from its anionic adsorption. © 1998, The Institute of Electrical Engineers of Japan. All rights reserved.
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Wang X., Sakai G., Shimanoe K., Miura N., Yamazoe N.
Sensors and Actuators, B: Chemical 45 ( 2 ) 141 - 146 1997.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Sensors and Actuators, B: Chemical
Thin films of SiO2-WO3composites with various SiO2contents (0-20 wt%) were prepared by means of sol-gel method. The grain size of WO3decreased with increasing SiO2content, resulting in an increase in NO2sensitivity. On the other hand, the response transient to NO2was brought to be the quickest at 5% SiO2. The thin film of SiO2(5%)-WO3was far more sensitive to NO2than the thin film or sintered block of pure WO3. It could detect dilute NO2(0.1-2 ppm) in air at 350°C sensitively and fairly selectively. The NO2sensing characteristics were stable over the whole test period of 10 days. © 1997 Elsevier Science S.A.
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Spin-coated Thin Film of SiO2-WO3 Composites for Detection of Sub-ppm NO2 Reviewed
共著者：X. Wang, G. Sakai, K. Shimanoe, N. Miura, N. Yamazoe
Sensors and Actuators B, Vol. 45, 141-146 45 141 - 146 1997.12
Language：English Publishing type：Research paper (scientific journal)
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Detection of Morphine in ppb Range by Using SPR (Surface-Plasmon Resonance) Immunosensor Reviewed
共著者：N. Miura, K. Ogata, G. Sakai, T. Uda N. Yamazoe
Chem. Lett., Vol. 1997, No.8, 713-714 1997 ( 8 ) 713 - 714 1997.8
Language：English Publishing type：Research paper (scientific journal)
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Sakai G., Saiki T., Uda T., Miura N., Yamazoe N.
Sensors and Actuators, B: Chemical 42 ( 2 ) 89 - 94 1997.7
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Sensors and Actuators, B: Chemical
Binding of human serum albumin (HSA) to its monoclonal and polyclonal antibody was examined by using a quartz crystal immunosensor on which the immobilized monoclonal antibody was exposed to HSA and polyclonal antibody stepwise. The association constants and rate constants of the respective steps of immunoreactions were estimated from the resonant frequency shifts at steady state and the time course of resonant frequency. The binding of HSA to the polyclonal antibody (second step) was shown to be much weaker and slower than that to the monoclonal antibody (first step), probably owing to the steric hindrance and orientation effects of the HSA already bound to the immobilized monoclonal antibody. © 1997 Elsevier Science S.A.
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Evaluation of Binding of Human Serum Albumin (HSA) to Monoclonal and Polyclonal-Antibody by means of Piezoelectric Immunosensing Technique Reviewed
共著者：G. Sakai, T. Saiki, T. Uda, N. Miura, N. Yamazoe
Sensors and Actuators B, Vol. 42, 89-94 42 89 - 94 1997.7
Language：English Publishing type：Research paper (scientific journal)
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Detection of morphine in ppb range by using SPR (surface-plasmon-resonance) immunosensor
Miura N., Ogata K., Sakai G., Uda T., Yamazoe N.
Chemistry Letters ( 8 ) 713 - 714 1997
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Chemistry Letters
Highly sensitive detection of morphine (MO) in ppb range was realized by using an immunosensor based on surface-plasmon-resonance (SPR) phenomena. The addition of MO into the antibody solution was found to reduce the incident angle shift of the SPR sensor system sharply because of the inhibition effect of MO. Based on this inhibiting principle, the present sensor could detect MO very sensitively in the concentration range of 0.1 - 10 ng/cm3(ppb).
DOI： 10.1246/cl.1997.713
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Highly sensitive detection of morphine by using immunosensor based on surface-plasmon-resonance
Sakai G., Ogata K., Uda T., Miura N., Yamazoe N.
International Conference on Solid-State Sensors and Actuators, Proceedings 1 179 - 182 1997
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：International Conference on Solid-State Sensors and Actuators, Proceedings
Highly sensitive and selective detection of morphine (MO) was realized by coupling an immunoreaction of an anti-MO monoclonal antibody and a surface-plasmon-resonance (SPR) phenomena. A normorphine-bovine serum albumin (NM-BSA) conjugate prepared as an antigen was immobilized on the Au thin film of sensor chip by physical adsorption. The incident angle of the SPR system using the NM-BSA immobilized chip increased almost linearly with increasing concentration of antibody up to ca. 5 μg/ml (ppm). The addition of MO into the antibody solution (5 ppm) was found to reduce the incident angle shift sharply because of the inhibition effect of MO. Based on this inhibiting principle, the present sensor could detect MO very sensitively in the concentration range of 0.1 - 10 ng/ml (ppb).
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水晶振動子を用いたガスおよびバイオセンサに関する研究
酒井 剛
学位論文 1996.2
Language：English Publishing type：Research paper (other academic)