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安全衛生保健センター |
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講師 |
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Ecotropic viral integration site 1 regulates EGFR transcription in glioblastoma cells 査読あり
Asako Mizuguchi , Shinji Yamashita, Kiyotaka Yokogami, Kazuhiro Morishita, Hideo Takeshima
journal of neuro-oncology 2019年10月
記述言語：英語 掲載種別：学位論文（博士）
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Impact of PCR-based molecular analysis in daily diagnosis for the patient with gliomas
Yokogami K., Yamasaki K., Matsumoto F., Yamashita S., Saito K., Tacheva A., Mizuguchi A., Watanabe T., Ohta H., Takeshima H.
Brain Tumor Pathology 35 ( 3 ) 141 - 147 2018年7月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Brain Tumor Pathology
© 2018, The Japan Society of Brain Tumor Pathology. The WHO2016 CNS update requires a combined histological and molecular assessment. To assess the major aberrations such as co-deletion of complete chromosome arms 1p and 19q (Co-del), isocitrate dehydrogenase and histone H3 mutations, direct sequencing, multiplex ligation-dependent probe amplification and/or FISH are methods considered to be “golden standard” in the community. However, these methods are expensive and complicated. The aim of this study is verification of the sensitivity of the simple PCR-based techniques for assessment of molecular information in daily diagnosis. We analyzed a total number of 80 patients with gliomas. FISH and PCR-based microsatellite analysis were compared for Co-del assessment. Direct sequencing and qPCR using hig-resolution melting (HRM) were compared for IDH and histone H3 mutations. The sensitivity and specificity of FISH were 0.71 and 0.79, respectively. FISH using a commercially available Vysis probe had a risk of high false-positive rate (0.25). For assessment of IDH1 mutations, the sensitivity and specificity of HRM were 1.0 and 0.96, respectively. For assessment of IDH2 and H3 mutations by HRM, both sensitivity and specificity were 1.0. We consider PCR-based molecular analysis to be a simple and accurate technique in daily diagnosis that is readily available for a small scientific facility.
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Yamashita S., Yokogami K., Matsumoto F., Saito K., Mizuguchi A., Ohta H., Takeshima H.
Journal of neurosurgery 130 ( 3 ) 780 - 788 2018年5月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of neurosurgery
In BriefThe authors assessed MGMT promoter methylation results obtained by methylation-sensitive PCR (MS-PCR) and high-resolution melting (MS-HRM) methods to determine whether MS-HRM overcomes the limitations of MS-PCR. They found that MS-HRM was superior to MS-PCR for predicting survival outcome in 75 GBM patients with and without MGMT promoter methylation. Based on the results of multivariate Cox analysis, MS-HRM revealed independent prognostic factors. The authors suggest that MS-HRM is optimal for assessing the MGMT promoter methylation status and that it represents an alternative to MS-PCR.
科研費（文科省・学振・厚労省）獲得実績 【 表示 ／ 非表示 】
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EVI1によるPDGFRβ転写制御とグリオブラストーマ血管新生機構について
研究課題/領域番号：22K09237 2022年04月 - 2027年03月
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
担当区分：研究代表者
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代謝経路再編成が概日リズム経路を介し、幹細胞性維持、細胞死回避に及ぼす影響の解明
研究課題/領域番号：22K09262 2022年04月 - 2025年03月
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
担当区分：研究分担者