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Affiliation |
Faculty of Agriculture Department of Veterinary Science Veterinarian physiologic course |
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Professor |
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External Link |
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NAKAHARA Keiko
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Research Areas 【 display / non-display 】
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Life Science / Veterinary medical science
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Life Science / Neuroscience-general
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Life Science / Physiology
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Life Science / Clinical pharmacy
Papers 【 display / non-display 】
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Tanaka Y, Taguchi S, Maruyama K, Mori K, Miyazato M, Kangawa K, Murakami N, Nakahara K
Biochemi Biophys Res Commun. 534 653 - 658 2021.1
Language：English Publishing type：Research paper (scientific journal) Publisher：Biochemical and Biophysical Research Communications
Two novel peptides, neuromedin U precursor-related peptide (NURP) and neuromedin S precursor-related peptide (NSRP), are produced from neuromedin U (NMU) and neuromedin S (NMS) precursors, respectively, as these precursors have multiple consensus sequences for proteolytic processing. Our group has shown previously that one of these two novel peptides, NURP, stimulates body temperature and locomotor activity, but not food intake. However, the physiological function of the other peptide, NSRP, has remained unclear. Therefore, the aim of this study was to characterize differences in the regions of the rat brain targeted by the NMU/NMS peptide family, including NURP and NSRP, and their physiological functions. First, we explored the regions of c-Fos expression after intracerebroventricular (i.c.v.) injection of NURP and NSRP and found that these were fewer than after i.c.v. injection of NMU and NMS in the hypothalamus, possibly because NURP and NSRP cannot activate NMU/NMS receptors. In the ventral subiculum, which is one region of the hippocampus, c-Fos expression was evident only after i.c.v. injection of NURP. We also examined the effects of NSRP on food intake, body temperature and locomotor activity. Like NURP, NSRP increased both body temperature and locomotor activity, but not food intake, indicating that NSRP is also a functional peptide. However, these effects of NSRP were distinctly weaker than those of NURP. These findings suggest differences in the affinity of NURP and/or NSRP for specific receptors, or in their respective biological activities.
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Neuromedin U suppresses prolactin secretion via dopamine neurons of the arcuate nucleus
Nakahara K., Maruyama K., Ensho T., Mori K., Miyazato M., Kangawa K., Uemura R., Sakoda H., Nakazato M., Murakami N.
Biochemical and Biophysical Research Communications 521 ( 2 ) 521 - 526 2020.1
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Biochemical and Biophysical Research Communications
Neuromedin U (NMU) has a precursor that contains one additional peptide consisting of 33 or 36 amino acid residues. Recently, we identified this second peptide from rat brain and designated it neuromedin U precursor-related peptide (NURP), showing it to stimulate prolactin release from the pituitary when injected via the intracerebroventricular (icv) route. Here, we examined whether NMU, like NURP, also stimulates prolactin release. Unlike NURP, icv injection of NMU significantly decreased the secretion of prolactin from the pituitary. This suppression of prolactin release by NMU was observed in hyper-prolactin states such as lactation, stress, pseudopregnancy, domperidone (dopamine antagonist) administration, and icv injection of NURP. Immunohistochemical analysis revealed that icv injection of NMU induced cFos expression in dopaminergic neurons of the arcuate nucleus, but not the substantia nigra. Mice with double knockout of NMU and neuromedin S (NMS), the latter also binding to NMU receptors, showed a significant increase of the plasma prolactin level after domperidone treatment relative to wild-type mice. These results suggest that NMU and NURP may play important reciprocal roles in physiological prolactin secretion.
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Purification and identification of native forms of goldfish neuromedin U from brain and gut. Reviewed
Maruyama K, Kaiya H, Miyazato M, Murakami N, Nakahara K, Matsuda K.
Biochemical Biophysical and Research Communications 517 433 - 438 2019.9
Language：English Publishing type：Research paper (scientific journal)
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Ensho T, Maruyama K, Qattali AW, Yasuda M, Uemura R, Murakami N, Nakahara K
The Journal of veterinary medical science 2019.7
Language：English Publishing type：Research paper (scientific journal) Publisher：公益社団法人 日本獣医学会
Recently, it has been proposed that neuromedin U (NMU) is "decretin", which suppresses insulin secretion from the pancreas <i>in vitro</i>. Here we examined the possible involvement of NMU in insulin secretion <i>in vivo</i> by comparing the plasma glucose and insulin levels of wild-type mice with those of double knockout (D-KO) of the NMU and neuromedin S (NMS) genes, as NMS binds to the neuromedin U receptor. If NMU is, in fact, "decretin", which inhibits insulin secretion from the pancreas, then NMU-deficient mice might result in higher plasma insulin levels than is the case in wild-type mice, or injection of NMU lead to suppression of plasma insulin level. In this study, we found that the fasting plasma level of insulin was not increased in D-KO mice. Glucose tolerance tests revealed no significant difference in plasma insulin levels between wild-type mice and D-KO mice under non-fasting conditions. After peripheral injection of NMU, plasma glucose and insulin levels did not show any significant changes in either wild-type or D-KO mice. Glucose tolerance testing after 3 weeks of high fat feeding revealed no significant difference in plasma insulin levels during 60 min after glucose injection between wild-type and D-KO mice. These results suggest that even if NMU is a decretin candidate, its physiological involvement in suppression of insulin secretion may be very minor <i>in vivo</i>.
DOI： 10.1292/jvms.19-0320
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Immunostimulatory effect of kumquat (Fortunella crassifolia) and its constituents, β-cryptoxanthin and R-limonene. Reviewed
Terao R, Murata A, Sugamoto K, Watanabe T, Nagahama K, Nakahara K, Kondo T, Murakami N, Fukui K, Hattori H, Eto N
Food Function 10 38 - 48 2019.4
Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1039/c8fo01971a.
Books 【 display / non-display 】
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デュークス獣医生理学
中原桂子 他（ Role： Joint translator）
学窓社 2020.3
Total pages：901 Responsible for pages：648-680 Language：Japanese Book type：Textbook, survey, introduction
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大・中・小動物実験プロトコール
村上昇、中原桂子（ Role： Sole author , 4－5体表体温の測定、４－６マウスラットの脳室内投与法）
宮崎南印刷 2016.3
Language：Japanese Book type：Scholarly book
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Functions of Dietary Valine, as Revealed by Dietary Valine-Deficiency
Kenji Nagao, Makoto Bannai, Keiko Nakahara, Noboru Murakami（ Role： Joint author）
Nova Science Publishers, Inc. NY 2012.4
Language：English Book type：Scholarly book
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食餌誘導性肥満モデル(Diet Induced Obesity Model)
村上 昇、中原桂子、寒川賢治（ Role： Joint author）
日本臨床社 2010.3
Language：Japanese Book type：Scholarly book
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生理学実習マニュアル
中原桂子（ Role： Joint author）
学窓社 2009.10
Language：Japanese Book type：Textbook, survey, introduction
MISC 【 display / non-display 】
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泌乳期における摂食調節機構に関する研究
鈴木喜博、中原桂子、延生卓也、大亀吏江子、東 善行、黒瀬陽平、村上昇
栄養生理研究会報 59 ( 1 ) 2015.11
Language：Japanese Publishing type：Research paper, summary (national, other academic conference) Publisher：栄養生理研究会
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泌乳期における摂食調節機構に関する研究
鈴木喜博、中原桂子、延生卓也、大亀吏江子、東善行
栄養生理研究会報 52 ( 8 ) 2014.4
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：栄養生理研究会
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グレリンとニューロメジンＵの生理作用の探索と臨床応用への可能性について
中原桂子、村上 昇, 寒川賢治
獣医生化学雑誌 41 31 - 50 2005.2
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：獣医生化学会
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単一松果体細胞の培養法
村上 昇、中原桂子
生体の科学 1999.8
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：医学書院
Presentations 【 display / non-display 】
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偽妊娠ラットにおけるニューロメジンU(NMU)およびNMU前駆体関連ペプチドの発現様式
杉山和気、松居裕司、村上 昇、丸山 圭介、中原 桂子
第164回日本獣医学会 (筑波) 日本獣医学会
Event date： 2021.9.3
Language：Japanese Presentation type：Oral presentation (general)
Venue：筑波
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ニューロメジンS前駆体中の新規ペプチド（NSRP）の生理機能の探索
田口 史門、延生 卓也、森 健二、宮里 幹也、寒川 賢治、丸山 圭介、村上 昇、中原 桂子
第162回日本獣医学会 (筑波) 日本獣医学会
Event date： 2019.9.10
Language：Japanese Presentation type：Oral presentation (general)
Venue：筑波
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ニューロメジンUおよび前駆体関連ペプチドの側脳室投与後のc-Fos発現部位の比較
田中 倖恵、村上 昇、中原 桂子
第162回日本獣医学会 (筑波) 日本獣医学会
Event date： 2019.9.10
Language：Japanese Presentation type：Oral presentation (general)
Venue：筑波
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プロラクチン分泌調節の謎 Invited
村上 昇、中原桂子
平成30年度 日本獣医師会獣医学術会年次大会 (神奈川) 日本獣医師会
Event date： 2019.2.10
Language：Japanese Presentation type：Oral presentation (general)
Venue：神奈川
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ニューロメジンU（NMU）、ニューロメジンS（NMS）とそれらの前駆体関連ペプチドのプロラクチン分泌への影響について Invited
村上 昇、森 健二、宮里幹也、中原桂子、大谷 瞳、筆谷麻未、井田隆徳、丸山圭介
第91回日本内分泌学会 (宮崎) 日本内分泌学会
Event date： 2018.10.20
Language：Japanese Presentation type：Oral presentation (general)
Venue：宮崎
Awards 【 display / non-display 】
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第150日本獣医学会生理生化学分科会奨励賞
2010.9 日本獣医学会生理生化学分科会
髙田志織、中原桂子、長尾健児、坂内 慎、高橋迪雄、村上 昇
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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宮崎大学女性研究者奨励賞
2010.3 宮崎大学
中原桂子
Country：Japan
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第147回日本獣医学会ベストポスター賞
2009.4 日本獣医学会
飯村 真、中原桂子、村上 昇
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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第146回日本獣医学会生理生化学分科会奨励賞
2008.9 日本獣医学会生理生化学分科会
坂本拓己、中原桂子、村上 昇
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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第144回日本獣医学会生理生化学分科会奨励賞
2007.10 日本獣医学会生理生化学分科会
福元 香、中原桂子、村上 昇
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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新規ペプチド“NURP”と“NSRP”のトランスレーショナルリサーチ
Grant number：20H03153 2020.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
Authorship：Principal investigator
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GhrelinとNeuromedinＵ／Ｓの自律神経様作用の解明と応用への研究
2016.04 - 2020.03
科学研究費補助金 基盤研究(B)
Authorship：Principal investigator
GhrelinとNeuromedinＵ／Ｓの自律神経様作用の解明と応用への研究
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哺乳動物の中枢・末梢クロストークによる摂食と走行運動調節機構の分子生物学的研究
2014.04 - 2018.03
科学研究費補助金 基盤研究(B)
Authorship：Coinvestigator(s)
哺乳動物の中枢・末梢クロストークによる摂食と走行運動調節機構の分子生物学的研究
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新たに発見したデスアシルグレリンの体温低下作用に寄る家畜の熱中症防止の検証
2014.04 - 2016.03
科学研究費補助金
Authorship：Coinvestigator(s)
新規に確立した遺伝的内臓脂肪蓄積型肥満マウスの特徴と原因遺伝子の解析
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新たに発見したNU1およびNU2ペプチドの生理機能の探索
2012.04 - 2014.03
科学研究費補助金
Authorship：Coinvestigator(s)
新規に確立した遺伝的内臓脂肪蓄積型肥満マウスの特徴と原因遺伝子の解析
Other research activities 【 display / non-display 】
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201４年アスビオファーム共同研究報告書
2014.03 - 2015.03
201４年アスビオファーム共同研究報告書を作成した
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201４年味の素株式会社共同研究成果報告書
2013.12 - 2014.12
企業との共同研究の成果の報告書を作成した
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2013年味の素株式会社共同研究成果報告書
2013.12
企業との共同研究の成果の報告書を作成した
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2013年アスビオファーム共同研究報告書
2013.04 - 2014.03
2013年アスビオファーム共同研究報告書を作成した
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平成25年度循環器病研究開発事業報告
2013.04 - 2014.03
遺伝子改変動物による循環器病の分子機序解明を目指した基礎研究（分担者）の報告書を作成した