研究者詳細 - 日髙　勇一

Preliminary experience of initial and re-administration of nimustine as a new primary treatment for feline nasal lymphoma: a case report 査読あり

Goda Y., Hidaka Y., Goto M., Inoue Y., Kuroda K., Nakatake Y., Sato R., Satoh H.

Japanese Journal of Veterinary Research 72 ( 2 ) 56 - 64 2024年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Japanese Journal of Veterinary Research

A 5-year-old spayed female domestic short haired cat presented with chronic nasal obstruction. The cat was diagnosed with B-cell nasal lymphoma based on histological examination of biopsy specimens and intracranial invasion was suspected. We initiated treatment with nimustine (ACNU), known to pass the blood–brain barrier, as the primary protocol with prednisolone, with the owner’s consent. The ACNU dose was 20–25 mg/m² (4–5-week intervals, four or six administrations, two courses). The cat remained oligosymptomatic until day 962 after the frst dose and no serious adverse events were reported. The cat lived until day 1089. To our knowledge, this is the frst report of long-term survival achieved using ACNU as the primary treatment in a cat with nasal lymphoma.

DOI： 10.57494/jjvr.72.2_56

リンパ管造影検査により臨床診断したリンパ水腫の黒毛和種子牛の1例査読あり

土本彩花, 桐野有美, 山内詩乃, 佐藤裕之, 保田昌宏, 平井卓哉, 井上賀之, 日高勇一

日本獣医師会雑誌 76 ( 9 ) e208 - e212 2023年10月

担当区分：責任著者記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：公益社団法人日本獣医師会

8カ月齢，雌，体重213kgの黒毛和種牛が原因不明の両後肢浮腫を示した．本症例は一般状態や歩様に異常はみられなかった．血液検査では浮腫の原因となる疾患の存在を示唆する所見は認めなかった．各種臨床検査及び診断的治療の結果から，本症例の両後肢浮腫は，心臓疾患や栄養失調を含む全身性疾患や局所の炎症性や血管性によるものではなく，局所リンパ流障害に起因することが示唆された．両前肢腕節及び両後肢飛節の皮下に油性ヨード系造影剤を注入し，経時的にX線撮影を実施した．その結果，両前肢では造影剤のリンパ管内流入が確認されたのに対し，両後肢では見出されなかった．以上の所見から，本症例は臨床的にリンパ水腫と診断され，病理学的検査により本疾患であることが確認された．

DOI： 10.12935/jvma.76.e208

Successful Treatment of Central Nervous System Lymphoma with Combination Therapy of Nimustine and Prednisolone in Two Dogs 査読あり

Mizutani Y., Inoue Y., Goda Y., Mizutani S., Asanuma T., Miura N., Hidaka Y., Sato R., Satoh H.

Veterinary Sciences 10 ( 9 ) 2023年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Veterinary Sciences

Of intracranial tumors, primary central nervous system lymphoma (PCNSL) is rare in dogs. Herein, we describe our experience with two dogs (a 3-year-old intact female toy poodle and a 5-year-old spayed female toy poodle) that developed neurological symptoms. Magnetic resonance imaging (MRI) revealed intracranial disseminated lesions. Cerebrospinal fluid (CSF) examination revealed pleocytosis and B-cell monoclonal proliferation in both cases. PCNSL or secondary central nervous system lymphoma (SCNSL) was diagnosed on the basis of MRI findings and CSF examinations. Nimustine (ACNU) is a nitrosourea alkylating agent, a class of drugs that includes lomustine. Nimustine is mainly used to treat human intracranial neoplasia because of its high permeability across the blood-brain barrier. The dogs in this study were treated with combined chemotherapy comprising nimustine and prednisolone, which achieved complete or nearly complete remission of neurological symptoms and long-term survival (>2583 days and 1218 days), but with problematic adverse effects. We determined that the dose of nimustine for canine PCNSL or SCNSL with intravenous infusion was 25–30 mg/m2 every 3–4 weeks for a total of four times; however, the data were insufficient to determine the optimal regimen.

DOI： 10.3390/vetsci10090533

A retrospective analysis for criteria of surgical intervention in Japanese Black calves with respiratory signs caused by perinatal rib fracture 査読あり

Repair of a calcaneal fracture with a combination of internal and external fixation in a calf 査読あり

Inoue Y, Mogi W, Naik MV, Kim S, Kirino Y, Hidaka Y, Sato R.

Journal of Veterinary Medical Science 85 ( 7 ) 739 - 742 2023年5月

記述言語：英語掲載種別：研究論文（学術雑誌）

Kim S., Naik M.V., Kirino Y., Satoh H., Tsuzuki N., Inoue Y., Kuroda K., Hidaka Y.

Journal of Veterinary Medical Science 85 ( 1 ) 40 - 43 2023年1月

担当区分：責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Veterinary Medical Science

Medical records of 16 Japanese Black calves with respiratory signs associated with perinatal rib fracture were analyzed, retrospectively, to speculate criteria of surgical intervention for the disease. For this analysis, the severity of respiratory condition was classified into three grades: Grade 1, with no wheezing, Grade 2, with wheezing after excitement but not at rest and Grade 3, with wheezing at rest. Grade 1 (n=3) received only conservative management. Seven of Grade 2 (n=8) and all of Grade 3 (n=5) had surgical management. Clinical outcome was good in all of Grade 1, and the surgical success rate was 83% in other groups. Our result suggests that surgical intervention should be considered for cases with more the severity of Grade 2.

DOI： 10.1292/jvms.22-0332

Usefulness of Computed Tomography for cryptorchidism in bulls. 査読あり

Goda Y, Mizutani S, Mizutani Y, Kitahara G, Siswandi R, Wakabayashi K, Torisu S, Kaneko Y, Hidaka Y, Osawa T, Asanuma T, Sato R, Satoh H

The Journal of veterinary medical science advpub ( 0 ) 1430 - 1436 2022年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：公益社団法人日本獣医学会

Cryptorchidism is defined as the failure of the testis to descend into the scrotal position. Bulls with cryptorchidism have problems in both meat quality and husbandry management; thus, it is greatly important to accurately identify the retained testis and remove it during the early stage. Abdominal computed tomography (CT) was performed under general anesthesia in 34 bulls aged 3–9 months with cryptorchidism. All bulls underwent laparoscopic or incision approach for cryptorchidectomy, and 40 testes were dissected. The detection rates of retained testes were 64.5% in the abdominal cavity and 100% in the subcutaneous inguinal region, and the total detection rate was 72.5%. Furthermore, all cases in this study were suspected to have intra-abdominal cryptorchidism in primary care, but CT revealed that 22.5% of cases had cryptorchidism in the subcutaneous inguinal region. The CT value (mean ± standard deviation) of the retained testes was 20.96 ± 7.41 Hounsfield Unit, and the CT value and size of the retained testes showed a positive weak correlation with bovine age. Therefore, there is the demerit that general anesthesia and a huge device are necessary; nevertheless, CT is suggested to be useful in identifying the location of retained testes and selecting an appropriate surgical approach in bulls with cryptorchidism. Moreover, CT findings suggested that the maturation of the retained testes might depend not on the descending process but on age.

DOI： 10.1292/jvms.22-0124

Ultrasonographic evaluation of the caudal vena cava in dogs with right-sided heart disease 査読あり

Fujioka T., Nakamura K., Minamoto T., Tsuzuki N., Yamaguchi J., Hidaka Y.

Journal of Veterinary Cardiology 34 80 - 92 2021年4月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Veterinary Cardiology

Introduction/objectives: In humans with impaired right-sided cardiac function, the caudal vena cava (CVC) diameter serves as a marker of venous congestion. This study aimed to investigate whether ultrasonographic CVC variables could identify the presence of right-sided congestive heart failure (R-CHF) in dogs with right-sided heart disease (RHD). Animals: Fifty client-owned control dogs and 67 dogs with RHD were enrolled. The dogs with RHD were subdivided into the non-R-CHF (n = 43) and R-CHF (n = 24) groups. Materials and methods: We measured and compared the ultrasonographic CVC variables and echocardiographic variables among the groups. Receiver operating characteristic (ROC) curve analysis was performed to calculate the sensitivity and specificity of the variables at optimal cutoff values. Results: We obtained the highest accuracies of the ratio of the shortest diameter (SD) of the minimal CVC area to the aorta diameter (Ao) during inspiration [SD(min)/Ao] and of the ratio of SD(min) to the longest diameter of the minimal CVC area during inspiration [LD(min),SD/LD(min)], with high sensitivities, specificities, and an area under the ROC curve greater than 0.925. Conclusions: In addition to the echocardiographic assessment of right-sided cardiac function, the CVC variables in this study, especially SD(min)/Ao and SD/LD(min), would be useful diagnostic indices for identifying R-CHF in dogs with RHD.

DOI： 10.1016/j.jvc.2021.01.005

Radiographic diagnosis and surgical management for successful outcomes for osteochondrosis in japanese black calves 査読あり

Naik M.V., Kirino Y., Uemura R., Kim S., Inoue Y., Hidaka Y.

Journal of Veterinary Medical Science 83 ( 1 ) 151 - 157 2021年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Veterinary Medical Science

© 2021 The Japanese Society of Veterinary Science. Osteochondrosis (OC) is not well recognized in cattle. Japanese Black (JB) cattle are valued for their beef and studies regarding OC are scarce. Hence, the aim of this study was to retrospectively discuss clinical and radiographical findings, diagnosis and treatment options for OC in JB cattle for successful outcomes. Medical records of 11 calves affected with OC were reviewed, retrospectively. Clinical and radiographic findings, treatment and outcomes and postmortem examination findings were analyzed. All calves had lameness. Stifle (4/14) and tarsal (4/14) were the most affected joints. Subchondral bone lucencies were observed in all joints (14/14) and accompanied with sclerosis (10/14). Radiographic grading was performed in calves. Calves were treated surgically (8/11) or conservatively (1/11). Surgically treated calves were auctioned (5/8). Postmortem examination was performed (2/11). Gross findings revealed ulcerative lesion at articular surface. Histopathologically, cartilage layer was defected at lesion. Inflammation (2/2) and granulation tissue (1/2) was also observed. In conclusion, OC in JB cattle can be diagnosed on the basis of radiography with history and clinical signs in clinical setting. Surgery should be performed in mild cases for successful outcomes. A modified radiographic grading criteria for clinical cases may hold potential in evaluating prognosis and outcomes.

DOI： 10.1292/jvms.20-0310

Breathing pattern analysis in cattle using infrared thermography and computer vision 査読あり

Kim S., Hidaka Y.

Animals 11 ( 1 ) 1 - 11 2021年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Animals

© 2021 by the authors. Licensee MDPI, Basel, Switzerland. Breathing patterns can be considered a vital sign providing health information. Infrared thermography is used to evaluate breathing patterns because it is non-invasive. Our study used not only sequence temperature data but also RGB images to gain breathing patterns in cattle. Mask R-CNN was used to detect the ROI (region of interest, nose) in the cattle RGB images. Mask segmentation from the ROI detection was applied to the corresponding temperature data. Finally, to visualize the breathing pattern, we calculated the temperature values in the ROI by averaging all temperature values in the ROI. The results in this study show 76% accuracy with Mask R-CNN in detecting cattle noses. With respect to the temperature calculation methods, the averaging method showed the most appropriate breathing pattern compared to other methods (maximum temperature in the ROI and integrating all temperature values in the ROI). Finally, we compared the breathing pattern from the averaging method and that from the thermal image observation and found them to be highly correlated (R2 = 0.91). This method is not labor-intensive, can handle big data, and is accurate. In addition, we expect that the characteristics of the method might enable the analysis of temperature data from various angles.

DOI： 10.3390/ani11010207

Severe Degenerative Changes in Cryptorchid Testes in Japanese Black Cattle 査読あり

Fuke N., Kitahara G., Ito S., Van Diep N., Ping Teh A.P., Izzati U.Z., Myint O., Hirai T., Torisu S., Kaneko Y., Sato H., Hidaka Y., Osawa T., Yamaguchi R.

Veterinary Pathology 57 ( 3 ) 418 - 426 2020年5月

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：Veterinary Pathology

© The Author(s) 2020. This is a histopathologic and endocrinologic study of 6 calves diagnosed with cryptorchidism. Cases 1–3 were diagnosed as resembling testicular regression syndrome. In cases 1 and 2, the extracted tissue was a small, firm, gray-white mass, and there was lack of obvious testicular tissue in case 3. Histopathologically, the excised tissue in cases 1–3 was a fibrotic testicular remnant with inflammation, mineralization, hemosiderin-laden macrophages or lipofuscin-laden macrophages, and lack of germ cells and interstitial endocrine cells. These findings were compared with cases 4–6, which were diagnosed as testicular hypoplasia due to cryptorchidism. These cases had small but otherwise grossly unremarkable intra-abdominal testicular tissue and histologically had a few germ cells and sustentacular cells with arrested spermatogenesis and an increase in interstitial endocrine cells. Cases 1–3 had more severe degenerative changes compared with cases 4–6. In case 2, the average diameter of the seminiferous tubules was much smaller than in cases 4–6, and there were few tubule cross sections. Anti-Müllerian hormone (214 pg/ml) was detected in the plasma of case 2. Based on the macroscopic and histopathologic findings as well as endocrinologic profiles, the testicular degeneration in cases 1–3 was considered similar to that of testicular regression syndrome. In this condition, it is thought that a normally developing intra-abdominal testis undergoes degeneration due to heat or a vascular disorder such as torsion.

DOI： 10.1177/0300985820906891

Bilateral rostral mandibulectomy for treatment of necrotic mandibular open fractures in two Japanese Black cattle. 査読あり

Naik MV, Nishikawa G, Kirino Y, Inoue Y, Iwamoto K, Yamabe R, Nakayama T, Tsuzuki N, Hidaka Y

The Journal of veterinary medical science 82 ( 2 ) 204 - 208 2020年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：公益社団法人日本獣医学会

Two-month-old (Case 1) and one-year-old (Case 2) Japanese Black cattle presented with bilateral rostral mandibular open fractures. At presentation, the proximal edges of each fracture were discolored and had a severe stench, indicating necrosis caused by infection. In both cases, a bilateral rostral mandibulectomy over the symphysis was performed. Although the tongues of both animals prolapsed post-surgery, they had no significant problems with eating or drinking. Case 1 showed excellent growth during fattening, and Case 2 successfully became pregnant and gave birth. Rostral mandibulectomy can be an effective surgical option for the treatment of cattle with difficulty in internal or external fixation due to unfavorable necrotic cranial mandibular open fractures.

DOI： 10.1292/jvms.19-0160

Immunohistochemical Profile of Ameloblastic Carcinoma Arising from an Amyloid-Producing Odontogenic Tumour in a Miniature Dachshund 査読あり

Izzati U., Hidaka Y., Hirai T., Yamaguchi R.

Journal of Comparative Pathology 166 54 - 58 2019年1月

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Comparative Pathology

© 2018 Elsevier Ltd A 13-year-old female miniature dachshund was presented with a centrally-located sublingual mass in the rostral mandibular region. The focally ulcerated growth completely covered the left (305) and right (405) premolar teeth and partially covered the right canine teeth (404). A punch biopsy sample revealed neoplastic proliferation of odontogenic epithelium arranged in irregular cords with frequent comedo-like necrosis. Following the initial diagnosis of ameloblastic carcinoma, a bilateral rostral hemimandibulectomy was performed. Although the detailed examination of the resected mass was consistent with the initial diagnosis, it also contained birefringent congophilic, amelogenin-labelled amyloid deposits similar to an amyloid-producing odontogenic tumour (APOT) in 30–40% of the mass, in continuity with the ameloblastic carcinoma. All neoplastic cells had diffuse moderate expression of cytokeratin (CK) AE1/AE3 and CK5, diffuse mild expression of CK14 and multifocal moderate expression of CK19. Because the APOT-like growth in the mass was histologically benign, the tumour was diagnosed as an ameloblastic carcinoma arising from an APOT.

DOI： 10.1016/j.jcpa.2018.10.182

Local extensive granulomatous inflammation of the neck region and lymphangitis caused by Lichtheimia corymbifera infection in a Japanese Black calf 査読あり

副腎皮質癌の腫瘍栓が後大静脈から右心系にまで達した犬の1例査読あり

藤岡崇伯, 野村雅史, 田邊美加, 西田卓真, 吉村公仁子, 岩本希生, 岩崎大樹, 山口明子, 山口潤, 日高勇一

動物の循環器 52 ( 2 ) 61 - 67 2019年

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：日本獣医循環器学会

ウェルシュ・コーギー・ペンブローグ，15歳，避妊雌が，腹囲膨満と運動時の虚脱を主訴に来院した。本例は3年前に近医にて実施された腹部超音波検査において，副腎腫瘍の可能性を指摘され，トリロスタンの内服治療を受けていたが，1年前からは後大静脈内腫瘍塞栓とみなされる超音波所見を随伴していた。当院で実施した経胸壁心エコー図検査では，右傍胸骨左室流出路断面像にて右心腔内に可動性の大型異常構造物が描出された。さらに右傍胸骨右室流入路断面像では，異常構造物は後大静脈から右心室腔内までつながっていた。以上の検査所見から，右心腔内の異常構造物は副腎腫瘍の後大静脈内進展に起因する右心腔内腫瘍塞栓と仮診断した。飼い主は積極的な外科的治療を希望せず，本例は当院初診の10日後に呼吸困難により自宅で死亡した。死後の病理学的検索により，右心腔内の異常構造物は副腎皮質癌に由来する右心腔内腫瘍塞栓であることが確認された。

DOI： 10.11276/jsvc.52.61

Teh A., Hirai T., Ito S., Hidaka Y., Goto Y., Furukawa H., Sawada J., Yamaguchi R.

Medical Mycology Case Reports 21 37 - 40 2018年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Medical Mycology Case Reports

© 2018 The Authors A 7-month-old female Japanese Black calf developed elongated, nodular mass measuring 30 × 16 cm extended from the retropharyngeal region to mid lateral neck region. Histological examination revealed granulomatous lymphangitis with non-septate fungal hyphae recognized throughout the lesions. Fungal culture, DNA sequencing and molecular phylogenetic tree analysis confirmed the sequence of Lichtheimia corymbifera. The lymphogenous route was speculated to be the main route of fungal spread leading to the characteristic nodular appearance of this case.

DOI： 10.1016/j.mmcr.2018.04.001

Characterization of anti-Müllerian hormone in a case of bovine male pseudohermaphroditism 査読あり

Kitahara G., El-Sheikh Ali H., Teh A., Hidaka Y., Haneda S., Mido S., Yamaguchi R., Osawa T.

Reproduction in Domestic Animals 53 ( 3 ) 809 - 813 2018年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Reproduction in Domestic Animals

© 2018 Blackwell Verlag GmbH The current report aimed to characterize plasma anti-Müllerian hormone (AMH) in bovine male pseudohermaphroditism. The blood AMH concentration in a Japanese Black male pseudohermaphrodite calf was compared with pre- and post-pubertal male and female calves and castrated calves. The concentration in the case was higher than in post-pubertal males, castrated males, and pre- and post-pubertal female calves (p <.05), but similar to that in pre-pubertal male calves. After extraction of the testes, the concentration in the case dropped to a certain extent. The extracted testes expressed AMH, as detected by immunohistochemistry. This study is the first to show the characterization of AMH in a male pseudohermaphrodite calf. AMH levels in peripheral blood might be useful to diagnose male pseudohermaphroditism in cattle.

DOI： 10.1111/rda.13149