Research Fields, Keywords

Life science

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Graduating School 【 display / non-display


    University of Miyazaki   Faculty of Agriculture   Graduated

Graduate School 【 display / non-display


    University of Miyazaki  Graduate School, Division of Agriculture  Master's Course  Completed


    Hokkaido University    Doctor's Course  Completed

Field of expertise (Grants-in-aid for Scientific Research classification) 【 display / non-display

  • Animal production science

  • Veterinary medical science

  • Veterinary medical science

  • Gastroenterology


Papers 【 display / non-display

  • IncI1 Plasmid Associated with blaCTX-M-2 Transmission in ESBL-Producing Escherichia coli Isolated from Healthy Thoroughbred Racehorse, Japan

    Sukmawinata E., Uemura R., Sato W., Mitoma S., Kanda T. and Sueyoshi M.

    Antibiotics 2020, 9, 70; doi:10.3390/antibiotics9020070   7   2020.02  [Refereed]

    Joint Work

     View Summary

    In our previous study, extended spectrum β-lactamase (ESBL)-producing Escherichia coli (ESBLEC) were isolated from healthy Thoroughbred racehorse feces samples in Japan. Some ESBL genes were predicted to be located on the conjugative plasmid. PCR-based replicon typing (PBRT) is a useful method to monitor and detect the association of replicons with specific plasmid-borne resistant genes. This study aimed to evaluate the plasmid replicon associated with ESBLEC isolated from healthy Thoroughbred racehorses at Japan Racing Association Training Centers in Japan. A total of 24 ESBLECs isolated from 23 (10.8%) individual Thoroughbred racehorse feces samples were used in this study. ESBL gene transfer was performed using a conjugation assay. Then, replicon types of ESBLEC isolates and their transconjugants were determined using PBRT. Pulsed-field gel electrophoresis (PFGE) was performed to look at the clonality of the ESBLECs isolates. ESBLECs were detected from 10.8% of healthy Thoroughbred racehorses. The blaCTX-M-2 was identified as the dominant type of ESBL gene, followed by blaCTX-M-1 and blaTEM-116. In this study, only the blaCTX-M-2 and the IncI1 plasmid were transferred to transconjugants. The PFGE results showed that ESBL genes were distributed in diversity of ESBLECs. This finding suggested that the IncI1 plasmid was associated with the dissemination of blaCTX-M-2 in Thoroughbred racehorses in Japan.

  • Multidrug‐Resistant ESBL/AmpC‐Producing Klebsiella pneumoniae Isolated from Healthy Thoroughbred Racehorses in Japan

    Sukmawinata E., Uemura R., Sato W., Htun M.T. and Sueyoshi M.

    Animals 2020, 10, 369; doi:10.3390/ani10030369.   25   2020.02  [Refereed]

    Joint Work

     View Summary

    Extended-spectrum β-lactamase (ESBL)- and AmpC β-lactamase (AmpC)-producing Klebsiella spp. have become a major health problem, leading to treatment failure in humans and animals. This study aimed to evaluate the presence of ESBL/AmpC-producing Klebsiella spp. isolated from racehorses in Japan. Feces samples from 212 healthy Thoroughbred racehorses were collected from the Japan Racing Association Training Centers between March 2017 and August 2018. ESBL/AmpC-producing Klebsiella spp. were isolated using selective medium containing 1 µg/mL cefotaxime. All isolates were subjected to bacterial species identification (MALDI-TOF MS), antimicrobial susceptibility test (disk diffusion test), characterization of resistance genes (PCR), conjugation assay, and genetic relatedness (multilocus sequence typing/MLST). Twelve ESBL/AmpC-producing Klebsiella pneumoniae (ESBL/AmpC-KP) were isolated from 3.3% of horse samples. Antimicrobial resistance profiling for 17 antimicrobials showed all ESBL/AmpC-KP were multidrug-resistant (MDR). Only 1 isolate was confirmed as an ESBL producer (blaCTX-M-2-positive), whereas the other 11 isolates were plasmid-mediated AmpC (pAmpC) producers (blaCMY positive). On the basis of MLST analysis, the ESBL-KP isolate was identified as sequence type (ST)-133 and four different STs among AmpC-KP isolates, ST-145, ST-4830, ST-4831, and ST-4832, were found to share six of the seven loci constituting a single-locus variant. This is the first study to show K. pneumoniae carrying MDR pAmpC isolated from a racehorse.

  • A known subject and unknown homework about classical swine fever 2018 in Japan

    Masuo SUEYOSHI

    Proc. Jpn. Pig Vet. Soc.   75   1 - 2   2020.02  [Refereed]

    Single Work

  • Extended spectrum β-Lactamase producing Escherichia coli isolated from healthy thoroughbred racehorse in Japan

    Sukmawinata E., Sato W., Kanda T., Kusano K., Kambayashi Y., Sato T., Ishikawa Y., Goto Y., Uemura R. Sueyoshi M.

    Journal of Equine Science   30   47 - 53   2019.09  [Refereed]

    Joint Work

  • Total serum protein reference value for clinical diagnostic index of equine proliferative enteropathy

    Ueno Y., Uemura R., Niwa H., Higuchi T., Sekiguchi S., Sasaki Y. Sueyoshi M.

    Journal of Equine Science   30   63 - 67   2019.09  [Refereed]

    Joint Work

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Books 【 display / non-display

  • Diseases of birds

    (Part: Joint Work )


  • Mechanisms in the Pathogenesis of Enteric Diseases(共著)

    Sueyoshi, M., M. Nakazawa, S. Tanaka (Part: Joint Work )

    Plenum Press  1997.04 ISBN: 0306455196

Review Papers 【 display / non-display

  • Pandemic~African swine fever~

    The Journal of Biomedical Science and Biosafety   31 ( 4 ) 16 - 21   2019.09

    Introduction and explanation (scientific journal)   Single Work

Presentations 【 display / non-display

  • Swine production and health status in Japan

    Yamagami T., Miyama T., Toyomaki H., Sekiguchi S., Sasaki Y., Sueyoshi M. and Makita K.

    Asian Pig Veterinary Society Congress 2019  (Seoul, Korea)  2019.08  -  2019.08  Asian Pig Veterinary Society Congress 2019

     View Summary

    The swine industry in Japan showed a continuous reduction in number of swine farms, but the number of pigs per farm continued to rise. The swine industry in Japan has become an important agricultural industry. However, swine diseases are a major factor reducing swine productivity in Japan. Common diseases include PRRS, PCVAD, edema disease, and pleuropneumonia etc. Although cattle and pigs had a FMD outbreak in 2010, they are now free of FMD without vaccination. Recently, CSF, a viral disease, was positive in pigs and wild boars.

  • Analysis of the effect of feedback feeding on farm-level porcine epidemic diarrhea occurrence in Kagoshima and Miyazaki Prefectures in Japan

    Yamagami T., Miyama T., Toyomaki H., Sekiguchi S., Sasaki Y., Sueyoshi M. and Makita K.

    The 15th International Society for Veterinary Epidemiology and Economics  (Chiang Mai, Thailand)  2018.11  -  2018.11  International Society for Veterinary Epidemiology and Economics

  • Feedback immunization to gilts and sows infected with porcine epidemic diarrhea in relation to time to stability and the number of dead piglets in Japan

    Furutani A., Sekiguchi S., Sueyoshi M. and Sasaki Y.

    The 15th International Society for Veterinary Epidemiology and Economics  (Chiang Mai, Thailand)  2018.11  -  2018.11  International Society for Veterinary Epidemiology and Economics

  • Bovine endocarditis coursed by Mycoplasma bovis

    Kanda T., Suwanruengsri M., Sukmawinata E., Uemura R., Yamaguchi R., Sueyoshi M.

    Joint Congress of The 7th Meeting of the Asian Organization for Mycoplasmology, The 45th Meeting of the Japanese Society of Mycoplasmology  2018.05  -  2018.05  Asian Organization for Mycoplasmology and Japanese Society of Mycoplasmology

     View Summary

    Mycoplasma bovis infection is associated with several diseases, such as mastitis, pneumonia, otitis media and arthritis in cattle. However, no reports exist regarding bovine endocarditis associated with M. bovis. Ten adult Japanese Black beef cattle with endocarditis that presented with caseation abscesses attached to the mucosal faces of their left atrium. But no lesions in their heart valves or on the right sides of their hearts were identified. M. bovis was successfully isolated from the lesions, and M. bovis antigen was detected in necrotic cores, small abscess at the surface of the masses and infiltrating macrophages using immunohistochemical analysis. Our results indicate that the lesions may be caused by M. bovis alone in.

  • Effects of zinc for Enterotoxaemic Escherichia coli in vitro

    Sueyoshi M.

    10th International symposium on Shiga toxin (Verocytotoxin) producing Escherichia coli infections  2018.05  -  2018.05  Organization for Shiga toxin (Verocytotoxin) producing Escherichia coli infections

     View Summary

    Swine edema disease is caused by enterotoxaemic E. coli (ETEEC). ETEEC produces Shiga toxin 2e. Addition of zinc formulations to feed has been used to treat and prevent the disease, but the mechanism of the beneficial effect is unknown. The objective of the present study was to investigate the effects of zinc supplementation on bacterial growth, hemolysin production, and the Stx2e release by ETEEC in vitro. ETEEC strain MVH269 isolated from a piglet with edema disease was cultured with ZnO or with ZnCO3, each at up to 3,000ppm. There was no effect of zinc addition on bacterial growth. Nonetheless, the cytotoxic activity of Stx2e released into the supernatant was significantly attenuated in the zinc-supplemented media compared to that in the control, with the 50% cytotoxic dose values of 163.2±12.7, 211.6±33.1 and 659.9±84.2 after 24hrs of growth in the presence of ZnO, ZnCO3, or no supplemental zinc, respectively. The hemolytic zones around colonies grown on sheep blood agar supplemented with zinc were significantly smaller than those of colonies grown on control agar. Similarly, hemoglobin absorbance after exposure to the supernatants of ETEEC cultures incubated in sheep blood broth supplemented with zinc was significantly lower than that resulting from exposure to the control supernatant. These in vitro findings indicated that zinc formulations directly impair the factors associated with the virulence of ETEEC, suggesting a mechanism by which zinc supplementation prevents swine edema disease.

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Recommended URL 【 display / non-display

  • National Institute of Animal Health

    Agriculture, Forestry and Fisheries in General

  • Livestock-industry information network

    Agriculture, Forestry and Fisheries in General