HASHIGUCHI Takuyu

写真a

Affiliation

Faculty of Regional Innovation

Title

Assistant Professor

External Link

Degree 【 display / non-display

  • 農学 ( 2014.3   宮崎大学 )

Research Areas 【 display / non-display

  • Life Science / Bioorganic chemistry

  • Life Science / Food sciences

  • Life Science / Applied biochemistry

  • Environmental Science/Agriculture Science / Science in plant genetics and breeding

 

Papers 【 display / non-display

  • Construction of prediction models for growth traits of soybean cultivars based on phenotyping in diverse genotype and environment combinations. Reviewed

    Manggabarani AM*, Hashiguchi T*, Hashiguchi M, Hayashi A, Kikuchi M, Mustamin Y, Bamba M, Kodama K, Tanabata T, Isobe S, Tanaka H, Akashi R, Nakaya A, Sato S, *Equally contributed.

    DNA research   2022.8

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/dnares/dsac024

    PubMed

  • Development of a plant conveyance system using an AGV and a self-designed plant-handling device: A case study of DIY plant phenotyping Reviewed

    Tanabata Takanari, Kodama Kunihiro, Hashiguchi Takuyu, Inomata Daisuke, Tanaka Hidenori, Isobe Sachiko

    Breeding Science   72 ( 1 )   85 - 95   2022

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Japanese Society of Breeding  

    <p>Plant phenotyping technology has been actively developed in recent years, but the introduction of these technologies into the field of agronomic research has not progressed as expected, in part due to the need for flexibility and low cost. "DIY" (Do It Yourself) methodologies are an efficient way to overcome such obstacles. Devices with modular functionality are critical to DIY experimentation, allowing researchers flexibility of design. In this study, we developed a plant conveyance system using a commercial AGV as a case study of DIY plant phenotyping. The convey module consists of two devices, a running device and a plant-handling device. The running device was developed based on a commercial AGV (Automated Guided Vehicle) Kit. The plant-handling device, plant stands, and pot attachments were originally designed and fabricated by us and our associates. Software was also developed for connecting the devices and operating the system. The run route was set with magnetic tape, which can be easily changed or rerouted. Our plant delivery system was developed with low cost and having high flexibility, as a unit that can contribute to others' DIY' plant research efforts as well as our own. It is expected that the developed devices will contribute to diverse phenotype observations of plants in the greenhouse as well as to other important functions in plant breeding and agricultural production.</p>

    DOI: 10.1270/jsbbs.21070

    Scopus

    CiNii Research

  • Varietal differences in flavonoid and antioxidant activity in Japanese soybean accessions Reviewed

    Arifin H.A.*, Hashiguchi T.*, Nagahama K., Hashiguchi M., Muguerza M., Sakakibara Y., Tanaka H., Akashi R. *Equally contributed.

    Bioscience, biotechnology, and biochemistry   85 ( 4 )   916 - 922   2021.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Bioscience, biotechnology, and biochemistry  

    Soybean seeds contain many antioxidants, including flavonoids and other phytochemicals. Isoflavone is a phytoestrogen that mimics estrogenic effects on target tissues and also exerts antioxidant activity by sequestering free radicals. Despite many cultivars developed to date, varietal differences in flavonoid content and antioxidant activity in Japanese soybean accessions remain less well characterized. Here, we evaluated the seed content of isoflavones, total flavonoids, and total phenolics in 26 soybean accessions. Next, the antioxidant activity of each accession was examined using antioxidant responsive element (ARE) linked to a luciferase reporter in human HepG2 stable cells. The relative ARE luciferase activity rate of all soybean accessions varied up to 4-fold which ranged from 1.00 to 4.02; and 22 accessions exhibited significant antioxidant activities. Correlation analysis indicated that the level of total isoflavone moderately correlated with antioxidant activity.

    DOI: 10.1093/bbb/zbaa104

    Scopus

    PubMed

  • Quantitative analysis of seven plant hormones in Lotus japonicus using standard addition method Reviewed

    Hashiguchi, T, Hashiguchi, M, Tanaka, H, Fukushima, K, Gondo, T, Akashi, R

    PLoS One   16 ( 2 )   e0247276   2021.2

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    Language:English   Publishing type:Research paper (scientific journal)  

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  • Evaluation of seed amino acid content and its correlation network analysis in wild soybean (Glycine soja) germplasm in Japan Reviewed

    Chotekajorn A., Hashiguchi T., Hashiguchi M., Tanaka H., Akashi R.

    Plant Genetic Resources: Characterisation and Utilisation   19 ( 1 )   35 - 43   2021.2

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Plant Genetic Resources: Characterisation and Utilisation  

    Wild soybean (Glycine soja) is a valuable genetic resource for soybean improvement. Seed composition profiles provide beneficial information for the effective conservation and utilization of wild soybeans. Therefore, this study aimed to assess the variation in free amino acid abundance in the seeds of wild soybean germplasm collected in Japan. The free amino acid content in the seeds from 316 accessions of wild soybean ranged from 0.965 to 5.987 mg/g seed dry weight (DW), representing a 6.2-fold difference. Three amino acids had the highest coefficient of variation (CV): Asparagine (1.15), histidine (0.95) and glutamine (0.94). Arginine (0.775 mg/g DW) was the predominant amino acid in wild soybean seeds, whereas the least abundant seed amino acid was glutamine (0.008 mg/g DW). A correlation network revealed significant positive relationships among most amino acids. Wild soybean seeds from different regions of origin had significantly different levels of several amino acids. In addition, a significant correlation between latitude and longitude of the collection sites and the total free amino acid content of seeds was observed. Our study reports diverse phenotypic data on the free amino acid content in seeds of wild soybean resources collected from throughout Japan. This information will be useful in conservation programmes for Japanese wild soybean and for the selection of accessions with favourable characteristics in future legume crop improvement efforts.

    DOI: 10.1017/S1479262121000071

    Scopus

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MISC 【 display / non-display

  • 環境汚染とヘモグロビン~植物による組換えヘモグロビン研究の最前線~ Invited

    Takuyu Hashiguchi

    Farumashia   58 ( 6 )   608 - 608   2022.6

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    Authorship:Lead author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Publisher:The Pharmaceutical Society of Japan  

    DOI: 10.14894/faruawpsj.58.6_608

    CiNii Research

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  • NBRPミヤコグサ・ダイズにおけるバイオリソース整備 Invited

    橋口 正嗣,田中 秀典,橋口 拓勇,明石 良

    農業および園芸   96 ( 2 )   153 - 162   2021.2

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • ヒト cytosolic sulfotransferase発現大腸菌を用いた硫酸化ポリフェノール類の部位選択的合成とその応用

    下平武彦, 黒木勝久, 橋口拓勇, 水光正仁, 榊原陽一

    硫酸と工業   73 ( 11 )   127 - 133   2020.11

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    Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • Multiple functions of sulfate ion during the evolution

    58 ( 9 )   511 - 519   2020.9

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • Physiological significance of steroid sulfation by cytosolic sulfotransferases. Invited

    Hashiguchi T., Kurogi K., Suiko M., Sakakibara Y.

    72 ( 8 )   99 - 104   2019.8

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (other)  

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Presentations 【 display / non-display

  • シバ属における新たな形態計測法の検討 – 全周囲画像による3Dモデルの構築と活用–

    Sorawich Pongpiyapaiboon, 田中 秀典, 橋口 拓勇, 橋口 正嗣, 林 篤司, 七夕 高也, 兒玉 晋洋, 磯部 祥子, 明石 良

    日本育種学会第141回講演会  2022.3.20 

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    Event date: 2022.3.20 - 2022.3.22

    Language:English   Presentation type:Oral presentation (general)  

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  • 安定同位体を用いない標準添加法による植物ホルモンの定量

    福島公貴, 橋口拓勇, 橋口正嗣, 明石良

    植物微生物研究会  2021.9.9 

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    Event date: 2021.9.8 - 2021.9.10

    Presentation type:Poster presentation  

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  • 蛍光灯およびLED照明下におけるミヤコグサ実験系統の生育特性

    橋口正嗣, 橋口拓勇, 田中秀典, 明石良

    植物微生物研究会  2021.9.9 

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    Event date: 2021.9.8 - 2021.9.10

    Presentation type:Poster presentation  

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  • 標準添加法を用いたミヤコグサにおける植物ホルモン定量法の確立

    橋口 拓勇, 福島 公貴, 橋口 正嗣, 田中 秀典, 権藤 崇裕, 明石 良

    日本農芸化学会  2021.3 

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    Event date: 2021.3.18 - 2021.3.21

    Presentation type:Oral presentation (general)  

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  • ダイズおよびツルマメのプロテオーム解析

    橋口 拓勇,橋口 正嗣,田中 秀典,権藤 崇裕,明石 良

    日本農芸化学会 2020年度大会  (福岡) 

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    Event date: 2020.3.25 - 2020.3.28

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡  

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Awards 【 display / non-display

  • Featured home page article

    2016.9   Molecular Endocrinology  

    Hashiguchi, T., Arakawa, S., Takahashi, S., Gonzalez J.F., Sueyoshi, T., Negishi, M.

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    Award type:Honored in official journal of a scientific society, scientific journal  Country:Japan

  • 優秀ポスター賞

    2013.6   P450, UGT, SULT研究会 (現:内外環境応答・代謝酵素研究会)  

    橋口拓勇

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  • Young Investigator Award

    2011.8   日本フードファクター学会(JSoFF)  

    橋口拓勇

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

Grant-in-Aid for Scientific Research 【 display / non-display

  • 植物における硫酸転移酵素の生理機能に関する研究

    2012.04 - 2014.03

    科学研究費補助金  特別研究員奨励費

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    Authorship:Principal investigator 

Other research activities 【 display / non-display

  • 日本分子生物学会

    2019.12

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    ミヤコグサ・ダイズバイオリソース(NBRP)の展示

  • 日本プロテオーム学会

    2019.07

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    ミヤコグサ・ダイズバイオリソース(NBRP)の展示

  • 日本農芸化学会

    2019.03

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    ミヤコグサ・ダイズバイオリソース(NBRP)の展示

  • 日本分子生物学会

    2018.11

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    ミヤコグサ・ダイズバイオリソース(NBRP)の展示

  • 日本植物学会

    2018.09

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    ミヤコグサ・ダイズバイオリソース(NBRP)の展示