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Affiliation |
Engineering educational research section Department of Applied Chemistry Program |
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Title |
Associate Professor |
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External Link |
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HIROSE Jun
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Research Areas 【 display / non-display 】
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Life Science / Applied microbiology
Papers 【 display / non-display 】
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Evolution of genetic architecture and gene regulation in biphenyl/PCB-degrading bacteria Reviewed
Hidehiko Fujihara, Jun Hirose, Hikaru Suenaga
Frontiers in Microbiolog 14 1168246 - 1168246 2023.6
Language：English Publishing type：Research paper (scientific journal)
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Jun Hirose
Microorganisms 11 ( 2 ) 438 2023.2
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Microorganisms
Integrative and conjugative elements (ICEs) are mobile DNA molecules that can be transferred through excision, conjugation, and integration into chromosomes. They contribute to the horizontal transfer of genomic islands across bacterial species. ICEs carrying genes encoding aromatic compound degradation pathways are of interest because of their contribution to environmental remediation. Recent advances in DNA sequencing technology have increased the number of newly discovered ICEs in bacterial genomes and have enabled comparative analysis of their evolution. The two different families of ICEs carry various aromatic compound degradation pathway genes. ICEclc and its related ICEs contain a number of members with diverse catabolic capabilities. In addition, the Tn4371 family, which includes ICEs that carry the chlorinated biphenyl catabolic pathway, has been identified. It is apparent that they underwent evolution through the acquisition, deletion, or exchange of modules to adapt to an environmental niche. ICEs have the property of both stability and mobility in the chromosome. Perspectives on the use of ICEs in environmental remediation are also discussed.
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Study on arsenic methylation properties of Cellulomonas sp. K31 Reviewed
Munetoshi Miyatake, Jun Hirose, Koichiro Shiomori, Haruhiko Yokoi
Resources Processing 68 ( 3 ) 117 - 123 2022.2
Language：English Publishing type：Research paper (scientific journal)
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A new ICEclc subfamily integrative and conjugative element responsible for horizontal transfer of biphenyl and salicylic acid catabolic pathway in the PCB-degrading strain Pseudomonas stutzeri KF716 Reviewed
Jun Hirose, Takahito Watanabe, Taiki Futagami, Hidehiko Fujihara, Nobutada Kimura, Hikaru Suenaga, Masatoshi Goto, Akiko Suyama and Kensuke Furukawa
Microorganisms 9 ( 12 ) 2462 2021.11
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Study on Arsenic Methyltransferase Expressed in Recombinant E. coli Reviewed
Munetoshi Miyatake, Koshiro Takase, Jun Hirose, Koichiro Shiomori, Haruhiko Yokoi
Resources Processing 67 ( 2 ) 80 - 85 2020.12
Language：English Publishing type：Research paper (scientific journal)
MISC 【 display / non-display 】
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Evolution of genetic architecture and gene regulation in biphenyl/PCB-degrading bacteria Reviewed
Fujihara H., Hirose J., Suenaga H.
Frontiers in Microbiology 14 2023
Publishing type：Rapid communication, short report, research note, etc. (scientific journal) Publisher：Frontiers in Microbiology
A variety of bacteria in the environment can utilize xenobiotic compounds as a source of carbon and energy. The bacterial strains degrading xenobiotics are suitable models to investigate the adaptation and evolutionary processes of bacteria because they appear to have emerged relatively soon after the release of these compounds into the natural environment. Analyses of bacterial genome sequences indicate that horizontal gene transfer (HGT) is the most important contributor to the bacterial evolution of genetic architecture. Further, host bacteria that can use energy effectively by controlling the expression of organized gene clusters involved in xenobiotic degradation will have a survival advantage in harsh xenobiotic-rich environments. In this review, we summarize the current understanding of evolutionary mechanisms operative in bacteria, with a focus on biphenyl/PCB-degrading bacteria. We then discuss metagenomic approaches that are useful for such investigation.
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どこまで改造できるのか？タンパク質は
廣瀬 遵
生物工学会誌 85 ( 10 ) 453 2007.10
Language：Japanese Publishing type：Research paper, summary (national, other academic conference) Publisher：日本生物工学会
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Properties of a new spore-bound enzyme from traditional Japanese fermentation food ‘natto’
Jun Hirose
Proceedings of the Commemorative International Symposium for 70th Aniversary of Sunchon National University 2005.5
Language：English Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution) Publisher：Sunchon National University
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有用微細藻類のスクリーニングと分離株の諸特性
共著者：廣瀬 遵、横井春比古
都市エリア産学官連携促進事業【都城盆地エリア】「バイオマスの高度徹底活用による環境調和型産業の創出」第１年度成果報告書、56-58頁 2005.4
Language：English Publishing type：Article, review, commentary, editorial, etc. (other) Publisher：宮崎県産業支援財団
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Microbial degradation of polychlorinated biphenyls (PCBs): biochemical and genetic bases
共著者：K.Furukawa, H.Suenaga, T.Watanabe, H.Fujiwara, J.Hirose
Molecular Genetics of Marine Organisms (Recent Advances in Marine Biotechnology, Vol. 10) Ed. by M. Fingerman and R. Nagabhushanam, 197-220 2004.1
Language：English Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：Science Publishers Ltd. Enfield (NH), USA
Presentations 【 display / non-display 】
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Database screening of integrated and conjugative elements (ICEs) from Pseudomonas strains that contribute to environmental remediation
2024.3.25
Event date： 2024.3.24 - 2024.3.27
Language：Japanese Presentation type：Oral presentation (general)
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細菌のα-トルイル酸誘導体代謝経路をコードする遺伝子群の構造および進化
Pseudomonas sp. LLC-1株による-トルイル酸およびその誘導体の分解経路をコードする遺伝子群の機能解析
日本生物工学会九州支部 第29回福岡大会 2023.12.3
Event date： 2023.12.3
Language：Japanese Presentation type：Oral presentation (general)
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Database screening of integrated and conjugative elements (ICEs) from Pseudomonas strains that contribute to environmental remediation
2023.9.22
Event date： 2023.9.22
Presentation type：Oral presentation (general)
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細菌のα-トルイル酸誘導体代謝経路をコードする遺伝子群の構造および進化
廣瀬 遵
第45回 蛋白質と酵素の構造と機能に関する九州シンポジウム 2023.9.7
Event date： 2023.9.7
Language：Japanese Presentation type：Poster presentation
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Pseudomonas属細菌由来の環境浄化に寄与する染色体組み込み型可動性遺伝因子(ICE)のin silico解析
藤波寛人 上原由楓 廣瀬 遵
九州微生物研究フォーラム2023 2023.9.1
Event date： 2023.9.1 - 2023.9.2
Language：Japanese Presentation type：Poster presentation
Awards 【 display / non-display 】
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15th International Biotechnology Symposium and Exhibition (IBS 2012) Organizing Committee Poster Award
2012.9 IBS2012 Organizing Committee
Jun Hirose
Award type：Award from international society, conference, symposium, etc. Country：Korea, Republic of
Grant-in-Aid for Scientific Research 【 display / non-display 】
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芳香環酸素添加酵素の機能改変とコンビナトリアルケミストリーへの利用
2006.04 - 2008.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
芳香環酸素添加酵素は、ベンゼン環などの芳香環に立体選択的に水 酸基を導入する酵素であり、本酵素が触媒する立体選択的な反応を利用することによ って化学合成が困難なモノ及びジヒドロキシ化合物の合成が可能である。本研究課題 では芳香環酸素添加酵素にランダムに変異を導入した変異酵素ライブラリーの中から、基質特異性及び反応特異性が野生型酵素とは異なる変異型酵素の取得を試みるとともに、新たに酵素に変異を導入することによって本酵素の基質特異性及び反応特性の改変を試みる。
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発酵法による木質系バイオマスからの高濃度燃料エタノール生産
2006.04 - 2008.03
科学研究費補助金 基盤研究(C)
Authorship：Coinvestigator(s)
組み換え大腸菌とエタノール耐性酵母の組み合わせにより、木質系バイマスから燃料エタノールを高濃度に生産する発酵システムの構築を試みる。
Available Technology 【 display / non-display 】
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微生物ゲノムの解析による芳香族化合物代謝経路の分子進化の解明
リグニンの化学・生物処理による変換および有効利用
ポリフェノールの酸化酵素による変換および高機能化Related fields where technical consultation is available：発酵生産、汚染土壌の修復、微生物・植物の同定および系統解析、酵素利用
Message：・「微生物」「酵素」に関するテーマであれば何でもご相談ください。