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Affiliation |
Faculty of Agriculture Region of Animal and Plant Biosciences |
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Title |
Associate Professor |
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External Link |
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Related SDGs |
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YAMAMOTO Akihiro
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Degree 【 display / non-display 】
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博士(農学) ( 2004.3 筑波大学 )
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修士(農学) ( 2001.3 筑波大学 )
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学士(農学) ( 1999.3 筑波大学 )
Research Areas 【 display / non-display 】
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Life Science / Plant nutrition and soil science
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Environmental Science/Agriculture Science / Environmental agriculture
Education 【 display / non-display 】
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University of Tsukuba Graduate School, Division of Agriculture
- 2004.3
Country：Japan
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University of Tsukuba Second Cluster of College
- 1999.3
Country：Japan
Qualification acquired 【 display / non-display 】
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Consultant Engineer (agriculture)
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Hazardous Material Handler (first kind)
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First Kind of Junior High School Teacher License
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First Kind of High School Teacher License
Papers 【 display / non-display 】
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Nitawaki Yoshikazu, Yasukochi Takaaki, Naono Shinya, Yamamoto Akihiro, Saeki Yuichi
Microbes and Environments 39 ( 4 ) ME24023 2024.12
Language：English Publishing type：Research paper (scientific journal) Publisher：Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles
We herein investigated the effects of salt (NaCl) stress on soybean nodulation by rhizobial strains. We specifically examined: (1) the effects of NaCl on nodule maturity and positioning by inoculating three rhizobial strains (<i>Bradyrhizobium diazoefficiens</i> USDA110<sup>T</sup>, <i>Bradyrhizobium elkanii</i> USDA31, and <i>Sinorhizobium fredii</i> USDA191) onto soybean variety CNS, (2) the effects of the NaCl treatment on isoflavones (daidzein and genistein) secretion by CNS, (3) the effects of the NaCl treatment on gene expression induced by daidzein and genistein in rhizobia, and (4) the effects of the NaCl treatment on rhizobial growth. The results obtained were as follows: (1) the NaCl treatment delayed nodule development and reduced nodulation on the primary root following the USDA110<sup>T</sup> inoculation, minimal sensitivity regarding nodule formation in the USDA 31 inoculation, and significantly increased the mature nodule number and nodules on the primary root following the USDA 191 inoculation. (2) The NaCl treatment significantly reduced the secretion of daidzein from soybean roots, but did not significantly affect that of genistein. (3) NaCl treatment induced a significant decrease in genistein-induced <i>nodC</i> expression in USDA110<sup>T</sup>, but not in USDA31, and also caused a significant reduction in daidzein-induced <i>nodC</i> expression, but not genistein-induced expression, in USDA191. (4) NaCl treatment reduced survivability under acidic conditions, but increased survivability under saline-alkaline conditions for USDA191 than bradyrhizobia. These results indicate that saline conditions give <i>S. fredii</i> a competitive advantage over <i>Bradyrhizobium</i> during soybean infection.
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Effect of Drought Stress and Short-term Recovery on Proline and Polyamine Metabolism in Lotus japonicus Reviewed International coauthorship
Lee Y., Yamamoto A., Ragaey M., Hashiguchi M., Akashi R., Saeki Y.
Agricultural Research 2024.12
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Agricultural Research
Environmental issues, particularly drought and soil salinity, severely limit plant productivity. This study aimed to examine the general reaction of legumes, specifically Lotus japonicus, against drought stress and their recovery following rehydration. After a 5-week culturing period, drought stress was simulated by withholding irrigation for 7 days. Stress response and recovery were evaluated through bioassays, and measurements of chlorophyll, proline and polyamine (stress reactants), and hydrogen peroxide and malondialdehyde (oxidative stress indices). Drought stress inhibited overall growth and decreased relative water content, but a recovery to control levels was observed after 8 h of re-watering. Hydrogen peroxide and malondialdehyde levels increased under drought stress, but showed no significant difference from control levels upon recovery. The accumulation of proline and polyamines was also observed, suggesting that they are effective responses to drought stress in plants. Furthermore, gene expression of key enzymes involved in proline and polyamine metabolism was regulated by stress conditions. By a short 8-h recovery period, the expression levels of metabolism-related genes of proline and polyamine returned to the pre-stress level, but the accumulation of functional substances was not induced. This study provides insight into the physiological and biochemical changes that occur in plants under drought stress and highlights the importance of proline and polyamine metabolism in plant adaptation to drought stress.
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Soybean gene expression correlated with symbiotic rhizobial nitrogen fixation activity Reviewed
Yano Shunichi, Takushima Teruya, Ezawa Tatsuhiro, Sugimura Yusaku, Yamamoto Akihiro, Saeki Yuichi
Plant Root 18 35 - 47 2024.12
Language：English Publishing type：Research paper (scientific journal) Publisher：Japanese Society for Root Research
Soybean (<i>Glycine max</i> L.) can establish a symbiotic relationship with rhizobia. However, little is known about the host plant genes that correlate with the nitrogen fixation activity of symbiotic rhizobia. In this study, we investigated soybean genes that correlate with bacterial symbiotic nitrogen fixation in two experiments. In the first experiment, soybean seeds were inoculated with two strains of soybean rhizobia exhibiting different nitrogen fixation activities and were grown in the field until the R1 stage. Subsequently, the roots and nodules were subjected to RNA-seq analysis to identify the expression of soybean genes related to bacterial nitrogen fixation. In the second experiment, to confirm host gene expressions dependent on bacterial nitrogen fixation activity, nod<sup><sup>+</sup></sup>/fix<sup><sup>－</sup></sup> (Δ<i>nif</i> gene) mutants and the wild type were inoculated into soybean, and gene expressions were evaluated by qRT-PCR. <i>GmNRT2.5</i>, a high-affinity nitrate transporter gene, was correlated with nitrogen fixation activity. qPCR analysis of roots and nodules inoculated with a nod<sup><sup>+</sup></sup>/fix<sup><sup>－</sup></sup> mutant revealed that <i>GmNRT2.5</i> on chromosome 8 (Glyma.08G284000: <i>GmNRT2.5</i> Chr8) was particularly correlated with nitrogen fixation activity in the root nodule section. Based on the results, it is suggested that <i>GmNRT2.5</i> Chr8 may function as a regulatory gene bridging nitrogen fixation and nitrate absorption from the soil. Therefore, <i>GmNRT2.5</i> Chr8 might be a useful host gene for estimating symbiotic nitrogen fixation activity.
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Land utilization types impacted the species diversity and population distribution of nodulating and non-nodulating rhizobia in the Philippines Reviewed International coauthorship
Mason M.L.T., De Guzman B.L.T., Mactal A.G., Yamamoto A., Saeki Y.
Rhizosphere 27 100743 2023.9
Language：English Publishing type：Research paper (scientific journal) Publisher：Rhizosphere
The influence of three land utilization types (LUT) on the diversity and population of soybean-nodulating rhizobia indicated how human activities affect the ecology of these microorganisms. This report revealed that based on LUT (LUT1-rice-based; LUT2-soybean-based; LUT3-legume-rice), different species of rhizobia can be more abundant than the other species. Targeting the 16S rRNA gene and the 16S-23S rRNA gene ITS region through Polymerase Chain Reaction - Restriction Fragment Length Polymorphisms (PCR-RFLP), it was found out that a majority of the nodulating rhizobia was classified under the genus Bradyrhizobium and the non-nodulating rhizobia was classified under the genus of Sinorhizobium. The species diversity of nodulating rhizobia on the LUT2 showed the lowest at 0.00 whereas the LUT1 showed the highest diversity at 0.87. Similarly, the lowest species diversity of the non-nodulating rhizobia was found on the LUT2 at 0.00 but the highest was obtained from the LUT3 at 0.69. These results suggest that the nodulating rhizobia in this study may have preference with rice-based cropping pattern than with the two cropping systems. On the other hand, the diversity of rhizobia observed on LUT2 indicated that soybean monocropping may have resulted to a lesser diversity of both nodulating and non-nodulating rhizobia. Thus, it can be stated that the change in land utilization even in an agriculture land-use, species diversification and population dominance of certain rhizobia may occur.
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Development of an effective method of human DNA extraction from soil as forensic evidence Reviewed International coauthorship
Hiroko Hirashima, Rinnosuke Hisazumi, Maria Luisa T. Mason, Akihiro Yamamoto, Yuichi Saeki
Forensic Science International 335 111284 2022.3
Language：English Publishing type：Research paper (scientific journal)
Books 【 display / non-display 】
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新植物栄養・肥料学 改訂版
山本昭洋（ Role： Joint author , トピックス2-5 チャの栄養生理と品質）
朝倉書店 2023.4
Language：Japanese Book type：Scholarly book
MISC 【 display / non-display 】
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能動的な技術者倫理教育の実践への取り組み
山本昭洋
宮崎大学教学マネジメント研究 1 35 - 49 2024.3
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution)
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Clarification of salt tolerance mechanisms and evaluation of adaptation to salt-accumulated soils of Zoysia spp. Invited
Agricultural Biotechnology 7 ( 1 ) 94 - 98 2023.1
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
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Clarification of salt tolerance mechanisms of Zoysia spp. and its application to sustainable global environment Invited
Agricultural Biotechnology 6 ( 5 ) 87 - 91 2022.5
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
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ダイズ根粒菌の生態研究を環境傾度対応型ダイズ生産技術へ Invited
佐伯雄一，山本昭洋
アグリバイオ 3 ( 12 ) 65 - 68 2019.10
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：北隆館
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ダイズ根粒菌生態研究から環境保全型ダイズ生産技術へ Invited
佐伯雄一，山本昭洋
アグリバイオ 2 ( 7 ) 92 - 95 2018.6
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：北隆館
Presentations 【 display / non-display 】
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窒素と亜鉛の異なる施肥量がチャ葉の成分におよぼす影響
永野 美希，河野美加，中嶋周作，児崎章憲，山本昭洋，佐伯雄一
2024年度日本土壌肥料学会九州支部例会
Event date： 2024.12.12 - 2024.12.13
Language：Japanese Presentation type：Oral presentation (general)
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塩ストレス条件下におけるS. frediiダイズ根粒菌の根粒着生優占化機構に関する研究
仁田脇義和，安河内孝晃，直野晋也，山本昭洋，佐伯雄一
2024年度日本土壌肥料学会九州支部例会
Event date： 2024.12.12 - 2024.12.13
Language：Japanese Presentation type：Oral presentation (general)
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塩ストレス条件下におけるシバ属の有用性評価
藤 康樹，山本昭洋，橋口正嗣，佐伯雄一，明石 良
2024年度日本土壌肥料学会九州支部例会
Event date： 2024.12.12 - 2024.12.13
Language：Japanese Presentation type：Oral presentation (general)
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ダイズ根粒菌混合接種による窒素固定量のコントロール
矢野俊一，山本昭洋，佐伯雄一
2024年度日本土壌肥料学会九州支部例会
Event date： 2024.12.12 - 2024.12.13
Language：Japanese Presentation type：Oral presentation (general)
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Change in growth and covering potential in the Zoysia spp. under salt stress conditions International conference
Koki Fuji, Akihiro Yamamoto, Masatsugu Hashiguchi, Yuichi Saeki, Ryo Akashi
The 16th JKTC International Student Seminar
Event date： 2024.11.11 - 2024.11.15
Language：English Presentation type：Oral presentation (general)
Awards 【 display / non-display 】
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令和4年度公募型卒業研究テーマ成果発表会ベストポスター賞
2023.3 宮崎県高等教育コンソーシアム 宮崎市内のビロウ並木の天狗巣・黄化・枯死症状の原因究明と対策
青山実樹，安達鉄矢，山本昭洋，佐伯雄一，櫛間義幸，土屋健一，竹下 稔
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日本土壌肥料学会九州支部奨励賞
2013.10 日本土壌肥料学会九州支部
山本昭洋
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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日本土壌肥料学会2012年度鳥取大会 ポスター賞
2012.9 日本土壌肥料学会
城惣吉, 山本昭洋, 梅原洋佐, 林正紀, 佐伯雄一
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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シバに耐塩性をもたらす生体成分の探索
Grant number：23K05492 2023.04 - 2026.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
Authorship：Principal investigator
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環境傾度に基づくフィリピンと日本の根粒菌ゲノム多様性と群集構造の比較解析 International coauthorship
Grant number：21KK0103 2021.10 - 2026.03
日本学術振興会 科学研究費基金 国際共同研究加速基金(国際共同研究強化(B))
Authorship：Coinvestigator(s)
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フィリピンの土着ダイズ根粒菌のゲノム多様性と群集構造解析
Grant number：18K05376 2018.04 - 2021.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship：Coinvestigator(s)
Available Technology 【 display / non-display 】
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Home Page： ・土壌肥料学研究室
Related fields where technical consultation is available：・植物（作物）の栄養診断（元素過剰や欠乏）
Message：・共同研究の希望テーマ：環境に左右されない植物の探索・開発
・「病気ではないのに植物(作物)に元気がない」などがあれば、ぜひ教えてください
