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Affiliation |
Faculty of Agriculture Department of Biochemistry and Applied Biosciences Living thing function science course |
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Title |
Associate Professor |
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External Link |
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YAMAMOTO Akihiro
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Degree 【 display / non-display 】
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博士(農学) ( 2004.3 筑波大学 )
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修士(農学) ( 2001.3 筑波大学 )
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学士(農学) ( 1999.3 筑波大学 )
Research Areas 【 display / non-display 】
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Life Science / Plant nutrition and soil science
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Environmental Science/Agriculture Science / Environmental agriculture
Education 【 display / non-display 】
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University of Tsukuba Graduate School, Division of Agriculture
- 2004.3
Country：Japan
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University of Tsukuba Second Cluster of College
- 1999.3
Country：Japan
Qualification acquired 【 display / non-display 】
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Consultant Engineer (agriculture)
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Hazardous Material Handler (first kind)
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First Kind of Junior High School Teacher License
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First Kind of High School Teacher License
Papers 【 display / non-display 】
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Land utilization types impacted the species diversity and population distribution of nodulating and non-nodulating rhizobia in the Philippines Reviewed International coauthorship
Mason M.L.T., De Guzman B.L.T., Mactal A.G., Yamamoto A., Saeki Y.
Rhizosphere 27 100743 2023.9
Language：English Publishing type：Research paper (scientific journal) Publisher：Rhizosphere
The influence of three land utilization types (LUT) on the diversity and population of soybean-nodulating rhizobia indicated how human activities affect the ecology of these microorganisms. This report revealed that based on LUT (LUT1-rice-based; LUT2-soybean-based; LUT3-legume-rice), different species of rhizobia can be more abundant than the other species. Targeting the 16S rRNA gene and the 16S-23S rRNA gene ITS region through Polymerase Chain Reaction - Restriction Fragment Length Polymorphisms (PCR-RFLP), it was found out that a majority of the nodulating rhizobia was classified under the genus Bradyrhizobium and the non-nodulating rhizobia was classified under the genus of Sinorhizobium. The species diversity of nodulating rhizobia on the LUT2 showed the lowest at 0.00 whereas the LUT1 showed the highest diversity at 0.87. Similarly, the lowest species diversity of the non-nodulating rhizobia was found on the LUT2 at 0.00 but the highest was obtained from the LUT3 at 0.69. These results suggest that the nodulating rhizobia in this study may have preference with rice-based cropping pattern than with the two cropping systems. On the other hand, the diversity of rhizobia observed on LUT2 indicated that soybean monocropping may have resulted to a lesser diversity of both nodulating and non-nodulating rhizobia. Thus, it can be stated that the change in land utilization even in an agriculture land-use, species diversification and population dominance of certain rhizobia may occur.
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Development of an effective method of human DNA extraction from soil as forensic evidence Reviewed International coauthorship
Hiroko Hirashima, Rinnosuke Hisazumi, Maria Luisa T. Mason, Akihiro Yamamoto, Yuichi Saeki
Forensic Science International 335 111284 2022.3
Language：English Publishing type：Research paper (scientific journal)
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Hafiz M.H.R., Salehin A., Adachi F., Omichi M., Saeki Y., Yamamoto A., Hayashi S., Itoh K.
Horticulturae 7 ( 2 ) 1 - 12 2021.2
Language：English Publishing type：Research paper (scientific journal) Publisher：Horticulturae
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. A species-specific latitudinal distribution of soybean rhizobia has been reported; Bradyrhi-zobium japonicum and B. elkanii dominate in nodules in northern and southern areas, respectively. The aim of this study was to elucidate whether temperature-dependent proliferation in soil or infection is more reliable for determining the latitudinal characteristic distribution of soybean-nodulating rhizobia under local climate conditions. Three study locations, Fukagawa (temperate continental climate), Matsue and Miyazaki (humid sub-tropical climate), were selected in Japan. Each soil sample was transported to the other study locations, and soybean cv. Orihime (non-Rj) was pot-cultivated using three soils at three study locations for two successive years. Species composition of Bradyrhi-zobium in the nodules was analyzed based on the partial 16S rRNA and 16S–23S rRNA ITS gene sequences. Two Bradyrhizobium japonicum (Bj11 and BjS10J) clusters and one B. elkanii (BeL7) cluster were phylogenetically sub-grouped into two (Bj11-1-2) and four clusters (BjS10J-1-4) based on the ITS sequence. In the Fukagawa soil, Bj11-1 dominated (80–87%) in all study locations. In the Matsue soil, the composition was similar in the Matsue and Miyazaki locations, in which BeL7 dominated (70–73%), while in the Fukagawa location, BeL7 decreased to 53% and Bj11-1 and BjS10J-3 increased. In the Miyazaki soil, BeL7 dominated at 77%, and BeL7 decreased to 13% and 33% in the Fukagawa and Matsue locations, respectively, while BjS10J-2 and BjS10J-4 increased. It was supposed that the B. japonicum strain preferably proliferated in the Fukagawa location, leading to its nodule domi-nancy, while in the Miyazaki location, temperature-dependent infection would lead to the nodule dominancy of B. elkanii, and both factors would be involved in the Matsue location.
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Mason M.L.T., De Guzman B.L.T., Yamamoto A., Saeki Y.
Symbiosis 83 ( 1 ) 55 - 63 2021.1
Language：English Publishing type：Research paper (scientific journal) Publisher：Symbiosis
© 2020, Springer Nature B.V. This report aimed to evaluate the ability of the dominant indigenous strains of bradyrhizobia obtained from different locations in the Philippines in terms of their symbiosis, N-fixation and nodulation with various soybean cultivars harboring different Rj genotypes. This was done to select the most efficient and effective strain that can be used as an inoculant under the Philippines’ local condition. Two soybean cultivars from the Philippines and three cultivars from Japan and Brazil were used and inoculated with 12 indigenous strains and the reference strain Bradyrhizobium diazoefficiens USDA110. Culture pots were grown inside a growth chamber for four weeks with 33 °C for 16 h as daytime and 28 °C for 8 h as night time. All the strains formed nodules on all soybean cultivars, except for B. elkanii BO-4, which only formed nodules on Philippines’ cultivars. Among the indigenous strains, B. elkanii IS-2 is significantly the most efficient and effective N-fixer than the other strains for the Philippines’ cultivars. In contrast, B. diazoefficiens SK-5 was found to be the most efficient and effective N-fixer for cultivars from Japan and Brazil. Additionally, a positive correlation was observed between the symbiotic efficiency and the nitrogenase activity indicating an efficient N-fixation by the indigenous strains. Thus, we were able to identify the most promising indigenous bradyrhizobia that could be used as an inoculant to increase the soybean yield in the Philippines and provided insights on soybean inoculation.
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Effect of salt stress on soybean growth and nodulation under inoculation with soybean rhizobia Reviewed
Nitawaki Y., Kitabayashi H., Mason M.L.T., Yamamoto A., Saeki Y.
Soil Science and Plant Nutrition 2020.12
Language：English Publishing type：Research paper (scientific journal) Publisher：Soil Science and Plant Nutrition
© 2020 Japanese Society of Soil Science and Plant Nutrition. Salinity is one of the major problems when it comes to soil fertility and agricultural productivity. In this regard, this study aimed to elucidate the influence of four salinity levels (0, 20, 50, 100 mM NaCl) on the soybean growth and the nodulation by rhizobia inoculated on the soybean variety CNS. Additionally, the compatibility of soybean varieties with the seven representative strains of rhizobia was also investigated. Through the compatibility test, it was revealed that the Sinorhizobium (Ensifer) fredii USDA 191 and the three bradyrhizobia (Bradyrhizobium japonicum USDA 6T, B. elkanii USDA 31, and B. diazoefficiens USDA 110T) showed compatible nodulation with the Rfg1 genotype soybean, whereas, the S. fredii USDA 192, 193, and 205T showed incompatible nodulation. The inoculation tests with bradyrhizobia (USDA 31, 110T) and sinorhizobia (USDA 191, 192, 193) indicated a better growth of soybean for bradyrhizobia than the sinorhizobia until 50 mM NaCl. Note that even though the symbiotic efficiency at each strain was not different among 0, 20, and 50 mM NaCl treatments, the symbiotic efficiency of bradyrhizobia was higher than the sinorhizobia at each NaCl treatment. The result of the mix inoculation test indicated that the nodule occupancy rate of strain USDA 191 increased significantly along with the NaCl concentration but strain USDA 110T showed an opposite response. These results indicate a positive relationship between the soybean growth and the nitrogen fixation ability of the inoculant under salt-stress conditions. Furthermore, the results obtained from the mix inoculation test partly describe the dominance of sinorhizobia under saline conditions.
Books 【 display / non-display 】
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新植物栄養・肥料学 改訂版
山本昭洋（ Role： Joint author , トピックス2-5 チャの栄養生理と品質）
朝倉書店 2023.4
Language：Japanese Book type：Scholarly book
MISC 【 display / non-display 】
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Clarification of salt tolerance mechanisms and evaluation of adaptation to salt-accumulated soils of Zoysia spp. Invited
Agricultural Biotechnology 7 ( 1 ) 94 - 98 2023.1
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
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Clarification of salt tolerance mechanisms of Zoysia spp. and its application to sustainable global environment Invited
Agricultural Biotechnology 6 ( 5 ) 87 - 91 2022.5
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
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ダイズ根粒菌の生態研究を環境傾度対応型ダイズ生産技術へ Invited
佐伯雄一，山本昭洋
アグリバイオ 3 ( 12 ) 65 - 68 2019.10
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：北隆館
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ダイズ根粒菌生態研究から環境保全型ダイズ生産技術へ Invited
佐伯雄一，山本昭洋
アグリバイオ 2 ( 7 ) 92 - 95 2018.6
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：北隆館
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根粒菌生態研究からみえる農業技術 日本におけるダイズ根粒菌の群集構造コントロールの可能性 Invited
佐伯雄一，山本昭洋
アグリバイオ 1 ( 10 ) 58 - 61 2017.9
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：北隆館
Presentations 【 display / non-display 】
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塩ストレス条件下のシバ属における被度変化
山本昭洋，齊藤七海，藤康樹，橋口正嗣，佐伯雄一，明石 良
第65回日本植物生理学会年会
Event date： 2024.3.17 - 2024.3.19
Language：Japanese Presentation type：Poster presentation
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塩ストレス条件下におけるシバの被度変化
山本昭洋，齊藤七海，橋口正嗣，佐伯雄一，明石 良
2023年度日本土壌肥料学会九州支部例会
Event date： 2023.12.14 - 2023.12.15
Language：Japanese Presentation type：Oral presentation (general)
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環境傾度によるダイズ感染根粒菌群集構造の比較解析 International coauthorship
長友由希，矢野俊一，Lee Yong Jae，Maria Luisa T. Mason，山本昭洋，佐伯雄一
2023年度日本土壌肥料学会九州支部例会
Event date： 2023.12.14 - 2023.12.15
Language：Japanese Presentation type：Oral presentation (general)
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ダイズ根粒菌混合接種による窒素固定活性コントロールの試み
矢野俊一，山本昭洋，佐伯雄一
2023年度日本土壌肥料学会九州支部例会
Event date： 2023.12.14 - 2023.12.15
Language：Japanese Presentation type：Oral presentation (general)
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窒素と亜鉛の施肥がチャの成分におよぼす影響
永野 美希，河野美加，中嶋周作，児崎章憲，山本昭洋，佐伯雄一
2023年度日本土壌肥料学会九州支部例会
Event date： 2023.12.14 - 2023.12.15
Language：Japanese Presentation type：Oral presentation (general)
Awards 【 display / non-display 】
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令和4年度公募型卒業研究テーマ成果発表会ベストポスター賞
2023.3 宮崎県高等教育コンソーシアム 宮崎市内のビロウ並木の天狗巣・黄化・枯死症状の原因究明と対策
青山実樹，安達鉄矢，山本昭洋，佐伯雄一，櫛間義幸，土屋健一，竹下 稔
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日本土壌肥料学会九州支部奨励賞
2013.10 日本土壌肥料学会九州支部
山本昭洋
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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日本土壌肥料学会2012年度鳥取大会 ポスター賞
2012.9 日本土壌肥料学会
城惣吉, 山本昭洋, 梅原洋佐, 林正紀, 佐伯雄一
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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シバに耐塩性をもたらす生体成分の探索
Grant number：23K05492 2023.04 - 2026.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究（C）
Authorship：Principal investigator
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環境傾度に基づくフィリピンと日本の根粒菌ゲノム多様性と群集構造の比較解析 International coauthorship
Grant number：21KK0103 2021.10 - 2026.03
日本学術振興会 科学研究費助成事業 国際共同研究加速基金(国際共同研究強化(B))
Authorship：Coinvestigator(s)
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フィリピンの土着ダイズ根粒菌のゲノム多様性と群集構造解析
Grant number：18K05376 2018.04 - 2021.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship：Coinvestigator(s)
Available Technology 【 display / non-display 】
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Home Page： ・土壌肥料学研究室
Related fields where technical consultation is available：・植物（作物）の栄養診断（元素過剰や欠乏）
Message：・共同研究の希望テーマ：環境に左右されない植物の探索・開発
・「病気ではないのに植物(作物)に元気がない」などがあれば、ぜひ教えてください
