KITAHARA Go

写真a

Affiliation

Faculty of Agriculture Department of Veterinary Science Animal clinical breeding study course

Title

Associate Professor

External Link

Degree 【 display / non-display

  • 博士(獣医学) ( 2012.9   山口大学 )

  • 学士(獣医学) ( 2002.3   鹿児島大学 )

Research Areas 【 display / non-display

  • Life Science / Veterinary medical science

 

Papers 【 display / non-display

  • Use of platelet lysate for in-vitro embryo production and treatment of repeat breeding in cows

    Hassaneen A.S.A., Rawy M.S., Yamanokuchi E., Elgendy O., Kawano T., Wakitani S., Kitahara G., Osawa T.

    Theriogenology   210   199 - 206   2023.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Theriogenology  

    Platelet-rich plasma (PRP) is a biological hemocomponent derived from blood after the complete removal of red blood cells and the partial or complete removal of white blood cells to concentrate platelets in an appropriate volume of plasma. Platelets have important growth factors, cytokines, and active metabolites that improve the endometrial environment and positively affect implantation. This study evaluated the effect of the addition of activated PRP (platelets lysate; PL) on in vitro bovine oocyte maturation and embryonic development and the effect of intrauterine (IU) infusion of autologous PL in repeat breeder (RB) cows. Experiment 1 examined the effects of allogeneic PL, fetal calf serum (FCS), mixed PL + FCS, or platelet-poor plasma (PPP) supplementations to in vitro maturation and development media on in vitro oocyte maturation and embryo development in good- and poor-quality oocytes of Japanese Black cows. Experiment 2 examined the IU infusion of autologous PL, 24 h post-insemination, in 21 RB Holstein-Friesian dairy cows. The cleavage rate of good-quality oocytes was higher in the PL group (85.93 ± 2.50%) than in the PPP group (67.16 ± 3.41%) (P < 0.05), while the cleavage rate of the poor-quality oocytes was higher in the PL alone (76.13 ± 4.04%) and mixed PL + FCS treated (73.59 ± 4.22%) groups than in the PPP group (54.64 ± 2.93%) (P < 0.05). The blastocyst rate of the good-quality oocytes was higher in the PL group (40.97 ± 3.03%) than in the FCS (27.97 ± 3.31%) and PPP (25.33 ± 2.15%) groups (P < 0.05). The blastocyst rate of poor-quality oocytes and the hatching rates of both good and poor-quality oocytes showed no significant differences among all groups. The conception rate in the autologous PL-treated group was 41.67% (5/12), while it was 11.11% (1/9) in the control group. The platelets' count in the pregnant PL-treated cows (n = 5; mean ± SEM, 1.07 ± 0.10 × 109/mL) was higher than in the non-pregnant ones (n = 7; 0.67 ± 0.10 × 109/mL) (P < 0.05). In conclusion, allogeneic PL was effective in stimulating the in vitro oocyte maturation and embryonic development in both good and poor-quality bovine oocytes, and post-insemination IU infusion of autologous PL derived from high platelets’ count-PRP would be recommended for the treatment of RB cows.

    DOI: 10.1016/j.theriogenology.2023.07.034

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  • 5-Aminolevulinic acid/sodium ferrous citrate improves the quality of heat-stressed bovine oocytes by reducing oxidative stress

    ELGENDY Omnia, KITAHARA Go, YAMADA Kentaro, TANIGUCHI Shin, OSAWA Takeshi

    Journal of Reproduction and Development   advpub ( 0 )   2023.8

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:The Society for Reproduction and Development  

    DOI: 10.1262/jrd.2023-038

    PubMed

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  • Usefulness of computed tomography for cryptorchidism in bulls Reviewed

    GODA Yoshimichi, MIZUTANI Shinya, MIZUTANI Yuko, KITAHARA Go, SISWANDI Riki, WAKABAYASHI Kenta, TORISU Shidow, KANEKO Yasuyuki, HIDAKA Yuichi, OSAWA Takeshi, ASANUMA Taketoshi, SATO Reiichiro, SATOH Hiroyuki

    Journal of Veterinary Medical Science   84 ( 10 )   1430 - 1436   2022.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:JAPANESE SOCIETY OF VETERINARY SCIENCE  

    <p>Cryptorchidism is defined as the failure of the testis to descend into the scrotal position. Bulls with cryptorchidism have problems in both meat quality and husbandry management; thus, it is greatly important to accurately identify the retained testis and remove it during the early stage. Abdominal computed tomography (CT) was performed under general anesthesia in 34 bulls aged 3–9 months with cryptorchidism. All bulls underwent laparoscopic or incision approach for cryptorchidectomy, and 40 testes were dissected. The detection rates of retained testes were 64.5% in the abdominal cavity and 100% in the subcutaneous inguinal region, and the total detection rate was 72.5%. Furthermore, all cases in this study were suspected to have intra-abdominal cryptorchidism in primary care, but CT revealed that 22.5% of cases had cryptorchidism in the subcutaneous inguinal region. The CT value (mean ± standard deviation) of the retained testes was 20.96 ± 7.54 Hounsfield Unit, and the CT value and size of the retained testes showed a positive weak correlation with bovine age. Therefore, there is the demerit that general anesthesia and a huge device are necessary; nevertheless, CT is suggested to be useful in identifying the location of retained testes and selecting an appropriate surgical approach in bulls with cryptorchidism. Moreover, CT findings suggested that the maturation of the retained testes might depend not on the descending process but on age.</p>

    DOI: 10.1292/jvms.22-0124

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  • Impact of dystocia and cow/calf characteristics on mortality from 0 to 120 days of age in Japanese Black calves in commercial cow-calf operations Reviewed

    Misaka M., Uematsu M., Hashimoto K., Kitahara G., Osawa T., Sasaki Y.

    Preventive Veterinary Medicine   207   105716   2022.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Preventive Veterinary Medicine  

    Calf mortality severely affects productivity in the beef industry. The present study was conducted to assess the calf mortality risk (CMR) in Japanese Black calves and investigate potential associations between calf/cow information and the CMR. Records for calves born between April 2006 and March 2010 were extracted from an existing database, which included production data on commercial cow-calf operations in Miyazaki, Japan. The study group comprised 40,462 calves born to 15,600 cows on 908 farms. Because calves on the studied farms were weaned at approximately 4 months of age, the calf records were collected from birth to 120 days. The CMR was calculated as the number of dead calves divided by the number of surviving calves. Mixed-effect multivariable logistic regression modeling was used to determine potential associations between the CMR and the factors hypothesized to influence that risk. CMR analyses were performed separately for the following production stages: days 0–30, days 31–60, days 61–90 and days 91–120 after birth. Of the 40,462 calves, 1465 died during days 0–120 after birth, and the CMR was 3.6 %. Of the 1465 dead calves, 35.9 % died within 10 days after birth. During the 0–30-day period, the CMR was associated with dam parity, calving status, gestation length, sex, birth season and twin births (P < 0.05), but not with the number of inseminations to conception. Low parity, dystocia, gestation lengths < 280 days and > 301 days, male calves, calves born in autumn and winter and twin births yielded higher CMRs. Low dam parity also yielded higher CMRs during the 31–60-day, 61–90-day and 91–120-day periods (P < 0.05), but no other factors were associated with the CMR during these periods. Thus, various cow and calf factors were shown to be associated with CMRs in Japanese Black calves, and calves with these factors should be considered high-risk calves.

    DOI: 10.1016/j.prevetmed.2022.105716

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  • Inflammatory Changes and Composition of Collagen during Cervical Ripening in Cows Reviewed

    Yamanokuchi E., Kitahara G., Kanemaru K., Hemmi K., Kobayashi I., Yamaguchi R., Osawa T.

    Animals   12 ( 19 )   2022.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Animals  

    Dystocia and stillbirths in cows pose a high risk of loss of both dams and fetuses, thereby resulting in high economic losses. One of the causes of these problems is birth canal abnormalities. Thus, to prevent these occurrences, it is necessary to understand the mechanisms underlying cervical ripening. Although physiological inflammatory responses and changes in collagen composition have been reported in humans and mice, related information is scarce for cows. We observed inflammatory changes and changes in the collagen composition in the cervix from late pregnancy to parturition to clarify some of the physiological changes associated with cervical ripening during normal calving in cows. Cervical mucus and tissue samples were collected from 41 Japanese Black cows at 200, 230, and 260 days of gestation and at 7-day intervals thereafter until parturition. The percentage of polymorphonuclear neutrophils (PMN%) in the mucus was calculated, and interleukin (IL)-8 concentration was determined by enzyme-linked immunosorbent assay. Blood samples were collected from the jugular vein, and leukocyte counts were determined. Picrosirius red-stained cervical tissue specimens were observed under a polarizing microscope, and the percentage of type I and type III collagen areas in the cervical tissue were calculated. The PMN% in cervical mucus was lowest at 200 days gestation (12–13 weeks before delivery), significantly increased 5 weeks before (21.7 ± 0.04), and was highest 1 week before calving (50.9 ± 0.04). IL-8 levels were increased at 295 days compared with those at 200 days of pregnancy (p < 0.05). No significant changes were observed in the white blood cell counts. The percentage of type I collagen in the cervical tissue reached a maximum (91.4 ± 0.02%) on day 200, significantly decreased after 274 days (3 weeks before calving), and continued to decrease thereafter until the week of parturition. There was no significant change in type III collagen levels. The results suggest that cervical ripening progresses when PMNs begin to infiltrate the cervix at around 260 days of gestation (5–4 weeks before parturition), IL-8, which increases at the end of pregnancy, mobilizes PMNs, and enhances inflammation, and that type I collagen changes are useful as an indicator of cervical ripening.

    DOI: 10.3390/ani12192646

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Books 【 display / non-display

  • 牛のマタニティハンドブック

    大澤健司・三宅陽一編( Role: Contributor ,  P40-45, 54-60)

    文永堂出版株式会社  2022.11 

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    Language:Japanese Book type:Scholarly book

  • 大・中・小動物実験プロトコール

    宮崎大学動物実験プロトコール編集委員会( Role: Joint author)

    宮日文化情報センター  2016.3 

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    Language:Japanese Book type:Scholarly book

  • 畜産ハンドブック 2.6 性の役割と性分化

    北原 豪( Role: Sole author)

    (株)講談社サイエンティフィク  2014.7 

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    Language:Japanese Book type:Dictionary, encyclopedia

  • 子牛の医学 第7章泌尿・生殖器疾患 第4節 生殖器の異常

    北原 豪( Role: Supervisor (editorial))

    緑書房  2014.3 

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    Language:Japanese Book type:Scholarly book

MISC 【 display / non-display

  • 海外テクニカルニュース:牛における帝王切開に関する総説:開腹へのアプローチと利点および欠点 Invited

    北原 豪

    臨床獣医   41 ( 3 )   70 - 71   2023.3

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    Authorship:Lead author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)   Publisher:緑書房  

  • 海外テクニカルニュース:ネロ―レ牛での人工授精後のプロジェステロン補充による黄体形成・機能と妊娠・妊娠喪失に及ぼす影響 Invited

    北原 豪

    臨床獣医   41 ( 2 )   70 - 71   2023.2

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    Authorship:Lead author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)   Publisher:緑書房  

  • 海外テクニカルニュース:未経産牛28症例における卵巣へのシリコンリングによる避妊手術後の経過観察 Invited

    北原 豪

    臨床獣医   41 ( 1 )   62 - 63   2023.1

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    Authorship:Lead author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)   Publisher:緑書房  

  • 海外テクニカルニュース:肉牛における定時胚移植プロトコールにおける性腺刺激ホルモンとしてのhCGの評価 Invited

    北原 豪

    臨床獣医   40 ( 12 )   68 - 69   2022.12

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    Authorship:Lead author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)   Publisher:緑書房  

  • 海外テクニカルニュース:セファピリンおよびPGF2αによる治療で臨床型子宮内膜炎から回復した乳牛における潜在性子宮内膜炎の存在 Invited

    北原 豪

    臨床獣医   40 ( 11 )   62 - 63   2022.11

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    Authorship:Lead author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)   Publisher:緑書房  

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Presentations 【 display / non-display

  • Effect of 5-Aminolevulinic acid combined with sodium ferrous citrate on the development of in vitro bovine oocytes in different seasons

    Omnia Abdelmaksoud Hassan Ali Elgendy,Go KITAHARA,Shin TANIGUCHI,Takeshi OSAWA

    第115回日本繁殖生物学会大会  (Web)  2022.9  日本繁殖生物学会

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    Event date: 2022.9.11 - 2022.9.14

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Web  

  • 黒毛和種経産牛の正常分娩に伴う子宮頸管熟化におけるマクロファージの動態

    金丸和之,北原 豪,小林郁雄,邊見広一郎,大澤健司

    第115回日本繁殖生物学会大会  (Web)  2022.9  日本繁殖生物学会

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    Event date: 2022.9.11 - 2022.9.14

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:Web  

  • ホルスタイン種経産牛の人工授精時における主席卵胞血流量と受胎性、およびその後に形成される黄体の血流量との関連

    太田智亨、齊藤史織、北原 豪、大澤健司

    第115回日本繁殖生物学会大会  (Web)  2022.9  日本繁殖生物学会

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    Event date: 2022.9.11 - 2022.9.14

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:Web  

  • Impacts of using platelet-rich plasma for in-vitro bovine embryo production and intra-uterine treatment of repeat breeder cows

    Ahmed Saad Ahned HASSANEEN,Mohamed Sadawy RAWY,Eigo YAMANAKUCHI,Omnia ELGENDY,Takanori KAWANO,Shoichi WAKITANI,Go KITAHARA,Takeshi OSAWA

    第115回日本繁殖生物学会大会  (Web)  2022.9  日本繁殖生物学会

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    Event date: 2022.9.11 - 2022.9.14

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Web  

  • 御崎馬における馬ヘルペスウイルス1型および4型の感染状況と流死産の関係性

    依田健寛、北原 豪、小林郁雄、楠田哲士、紙野瑞希、上村涼子、大澤健司

    第165回日本獣医学会学術集会  (Web)  2022.9  日本獣医学会

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    Event date: 2022.9.6 - 2022.9.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:Web  

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Awards 【 display / non-display

  • English Presentation Award

    2019.3  

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  • 平成30年度日本獣医師会獣医学術学会年次大会学会長賞

    2019.2   日本産業動物獣医学会  

    鳥巣至道、保坂悠歩、金子泰之、北原豪、日髙勇一、桐野有美、水谷真也、山本集士

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  • 平成30年度獣医学術九州地区学会産業動物獣医学会地区学会長賞

    2018.10   日本産業動物獣医学会九州地区学会  

    保坂悠歩、鳥巣至道、金子泰之、北原豪、佐藤裕之、水谷真也、山本集士、Benedict Huai Ern ONG、水谷祐子、桐野有美、日髙勇一、浅沼武敏、永延清和

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  • 平成29年家畜診療賞

    2018.2   全国農業共済協会  

    佐藤知広,北原 豪,大澤健司

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    Award type:Honored in official journal of a scientific society, scientific journal  Country:Japan

  • 平成29年度農学部表彰論文

    2017.12   宮崎大学農学部  

    El-Sheikh Ali, H., Kitahara, G., Takahashi, T., Mido, S., Sadawy, M., Kobayashi, I., Hemmi, K., Osawa, T.

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    Country:Japan

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Grant-in-Aid for Scientific Research 【 display / non-display

  • 牛の子宮頚管熟化機構の解明と分娩後早期の一過性炎症惹起による子宮疾患制御

    Grant number:21H02362  2021.04 - 2024.03

    独立行政法人日本学術振興会  科学研究費補助金  基盤研究(B)

    大澤 健司

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    Authorship:Coinvestigator(s) 

  • ウシ潜在性子宮内膜炎の病態解明と治療・予防ストラテジーの確立

    Grant number:16H05038  2016.04 - 2020.03

    科学研究費補助金  基盤研究(B)

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    Authorship:Coinvestigator(s) 

  • GhrelinとNeuromedin U/Sの自律神経様作用の解明と応用への研究

    Grant number:16H05043  2016.04 - 2020.03

    科学研究費補助金  基盤研究(B)

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    Authorship:Coinvestigator(s) 

  • バイオマーカーによる家畜の卵巣機能の質および量的評価法の確立

    Grant number:16K18805  2016.04 - 2019.03

    科学研究費補助金  若手研究(B)

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    Authorship:Principal investigator 

  • 抗ミューラー管ホルモンを用いた新しい繁殖管理技術の開発

    Grant number:25850209  2013.04 - 2016.03

    科学研究費補助金  若手研究(B)

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    Authorship:Principal investigator 

    これまで国内外で調べられていない雌ウシの春機発動時期と周産期におけるAMHの動態を明らかにし、初産分娩月齢や分娩後の空胎日数などの繁殖効率の指標となる繁殖成績との関係を解析して、AMHをバイオマーカーとした生時より備わっている繁殖能力や周産期における非生産時の繁殖機能を評価する新しい繁殖管理技術の開発に挑む。

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Available Technology 【 display / non-display