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Affiliation |
Faculty of Agriculture Region of Veterinary Science |
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Title |
Associate Professor |
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External Link |
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Related SDGs |
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KITAHARA Go
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Degree 【 display / non-display 】
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博士（獣医学） ( 2012.9 山口大学 )
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学士（獣医学） ( 2002.3 鹿児島大学 )
Research Areas 【 display / non-display 】
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Life Science / Veterinary medical science
Papers 【 display / non-display 】
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Dynamics of macrophages and inflammatory cytokine concentrations in the cervix of late pregnant cows Reviewed
Kanemaru K., Kitahara G., Hashiguchi T., Hemmi K., Kobayashi I., Osawa T.
Theriogenology 238 117357 2025.5
Language：English Publishing type：Research paper (scientific journal) Publisher：Theriogenology
Cervical ripening is associated with M1 macrophages and inflammatory cytokines in mice and rats, but its mechanism in cattle is unclear. In this study, to elucidate some of the mechanisms of normal cervical maturation in cows, we examined the expressions of M1 macrophages and mRNA of interleukin (IL)-1α, 1β, 6, 8, and 10, as well as TNFα in cervical tissue and the mRNA expressions and protein concentrations of these cytokines in cervical mucus from late pregnancy to parturition. Forty-one Japanese Black cows were sampled at 200, 230, and 260 d of gestation and then at 7 d intervals until parturition to obtain cervical mucus and tissue samples. The collected tissues were fluorescently immunostained with anti-Iba-1 antibodies, and the macrophage infiltration was checked. Cervical mucus was mixed with PBS, the concentrations of the above cytokines in the supernatant after centrifugation were measured, and the concentration per protein weight was used as the measurement. Total RNA was extracted from the cervical tissue and mucus, and the mRNA expression of various cytokines was analyzed using real-time PCR. In cervical tissue, macrophages stained with anti-Iba-1 antibody were observed from five to six weeks before calving until the week of calving. There was also a trend toward a concurrent increase in the mRNA expression of inflammatory cytokines, especially IL-6, three weeks before calving (P < 0.05). In cervical mucus, the concentrations of IL-1α, IL-1β, IL-8, and TNFα increased (P < 0.05) zero to three weeks before calving compared to 12–14 weeks before calving. In addition, IL-1α mRNA increased three weeks before and during the week of parturition, and IL-8 mRNA increased three weeks before and two weeks after (P < 0.05). These results suggest that cervical ripening in cows begins five to six weeks before calving when macrophages infiltrating cervical tissue may produce large amounts of IL-6, inflammatory cells infiltrate cervical mucus, and IL-1α, IL-1β, IL-8, and TNFα levels in the mucus increase toward calving.
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Suzuki O., Uematsu M., Kitahara G., Osawa T., Sasaki Y.
Reproduction in Domestic Animals 60 ( 4 ) e70058 2025.4
Language：English Publishing type：Research paper (scientific journal) Publisher：Reproduction in Domestic Animals
The calving interval (CI) is an important determinant of reproductive performance in Japanese Black cattle. The present study aimed to compare individual fertility traits among farms classified by CI. In total, 49,780 artificial insemination (AI) records for 7327 cows from 2011 to 2014 were obtained from 622 farms located in Miyazaki, Japan. The farms were classified into three groups (referred to as farm CI groups) using the lower and upper 25 percentiles of farm CI as thresholds. The mean CI of individual cows was 403.5 ± 70.9 days. The interquartile ranges of CI for individual cows were 49 days for short CI farms and 114 days for long CI farms. The interval between calving and the first AI was associated with farm CI group and parity (p < 0.05) but not with calving season and gestation length. The conception rate (CR) at the first AI in cows on long CI farms decreased as the interval from calving to the first AI decreased (p < 0.05). Additionally, no differences in CR among farm CI groups were observed for AI number ≥ 4. In long CI farms, cows with two AI treatments had longer interservice intervals than those for AI values of 3 and ≥ 4 (p < 0.05). In summary, fertility characteristics for long CI farms differed from those of farms with short or middle CI, and standard operating procedures need to be improved to decrease the CI.
DOI： 10.1111/rda.70058
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NARAMOTO Kanami, KITAHARA Go, NAZHAT Sayed Ahmad, YASUDA Masahiro, OSAWA Takeshi
Journal of Veterinary Medical Science 87 ( 1 ) 97 - 102 2025
Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE
To clarify the profile of endometrial cytokines and their relation to inflammation and fertility, mRNA expressions of interleukin (IL)-1α, -1β, -8, -10 and tumor necrosis factor (TNF)-α at weeks (W) 3, 5, and 7 postpartum were compared between Holstein cows without and with cytological endometritis and between early (≤90 days) and delayed (>90 days postpartum) conception cows. <i>IL-1α</i>, <i>IL-1β</i>, and <i>IL-8</i> expressions at W5 and W7 were significantly higher in cows with cytological endometritis than without. While <i>IL-1α</i>, <i>IL-1β</i>, <i>IL-8</i>, and <i>TNF-α</i> expressions decreased from W3 to W5 in all early conception cows, the percentage of cows decreasing these mRNA was significantly lower in delayed conception cows than early conception cows. Evaluating endometrial cytokine expression in dairy cows helps diagnose cytological endometritis and estimate fertility.
DOI： 10.1292/jvms.24-0177
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Maeda T., Kitahara G., Osawa T.
Veterinary Journal 305 106147 2024.6
Language：English Publishing type：Research paper (scientific journal) Publisher：Veterinary Journal
Preventing dystocia can stabilise beef cattle management. This study aimed to investigate the relationship between serum pregnancy-associated glycoproteins (PAGs) S-N values and estrone sulphate (E1S) concentrations during pregnancy and the calf birth weight in beef cattle and to evaluate their usefulness as new predictive parameters for dystocia due to foetal overgrowth. Thirty-eight pregnant Japanese Black cattle were used. Blood samples were collected at 40, 70, 100, 150, 200, 250, 280, and 285 days after artificial insemination (AI), and birth weight of the offspring was measured. Serum PAGs S-N values and E1S concentrations were measured, and the area under the curve (AUC) and the ratio of change based on 70 days after AI were calculated, followed by calculation of the correlation coefficient with the birth weight of the offspring and comparison between the eutocia (n = 32) and dystocia (n = 6) groups. The birth weight of the offspring was moderately positively correlated with the AUC of serum PAGs S-N values and E1S concentrations in the second (r = 0.425, P < 0.01) and third (r = 0.595, P < 0.01) trimesters, respectively. The ratio of change in serum E1S concentrations between 70 and 280 days after AI was greater (P < 0.05) in the dystocia group (1276.6 ± 229.1 %) than in the eutocia group (852.6 ± 69.6 %). These results suggest that blood PAGs S-N values at mid-pregnancy (100–199 days after AI) and the ratio of changes in blood E1S concentrations between 70 and 280 days after AI may be new parameters for predicting dystocia.
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Misaka M., Uematsu M., Kitahara G., Osawa T., Sasaki Y.
PLoS ONE 19 ( 6 June ) e0304867 2024.6
Language：English Publishing type：Research paper (scientific journal) Publisher：PLoS ONE
We aimed to characterize the change in the incidence of stillbirth (IS) in Japanese Black cattle during and after animal movement restrictions and suspended insemination because of a foot-and-mouth disease (FMD) outbreak in Miyazaki Prefecture in 2010. Calving data from 2006 to 2018 were collected from approximately 900 farms. Post-FMD period was divided into three based on the median IS per month (1.80%): period 1 (May 2011 to February 2013), period 2 (March 2013 to August 2015), and period 3 (September 2015 to December 2018). The ISs were similar during the Pre-FMD period and Post-FMD period 1, then substantially decreased during Post-FMD period 2 (p < .05), before returning to the value before the FMD outbreak period during Post-FMD period 3. Compared with the Pre-FMD period, Post-FMD period 1 was associated with a higher proportion of calvings by primiparous cows and Post-FMD period 2 was associated with a smaller number of calvings per month (p < .05). There were high ISs in primiparous cows during the Pre-FMD period, Post-FMD period 1, and Post-FMD period 3 (p < .05), but not during Post-FMD period 2. In summary, after the animal movement restrictions and suspended insemination introduced because of the FMD outbreak, the IS temporarily decreased and consequently returned to the pre-FMD level.
Books 【 display / non-display 】
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牛の臨床外科
編著者 佐藤礼一郎（ Role： Contributor）
株式会社緑書房 2025.4
Language：Japanese Book type：Scholarly book
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獣医繁殖学マニュアル
獣医繁殖学教育協議会（ Role： Contributor）
文永堂出版株式会社 2025.3 （ ISBN:978-4-8300-3294-3 ）
Language：Japanese Book type：Scholarly book
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獣医繁殖学第5版
獣医繁殖学教育協議会（ Role： Contributor , 泌乳の生理、生殖器の先天異常、泌乳の異常）
文永堂出版株式会社 2023.9
Language：Japanese Book type：Scholarly book
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牛のマタニティハンドブック
大澤健司・三宅陽一編（ Role： Contributor , P40-45, 54-60）
文永堂出版株式会社 2022.11
Language：Japanese Book type：Scholarly book
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大・中・小動物実験プロトコール
宮崎大学動物実験プロトコール編集委員会（ Role： Joint author）
宮日文化情報センター 2016.3
Language：Japanese Book type：Scholarly book
MISC 【 display / non-display 】
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海外テクニカルニュース：難産となったホルスタインにおける細菌学的負荷 Invited
北原 豪
臨床獣医 43 ( 3 ) 76 - 77 2025.3
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：緑書房
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海外テクニカルニュース：古くからの問題である牛の難産への対処 Invited
北原 豪
臨床獣医 43 ( 2 ) 72 - 73 2025.2
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：緑書房
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海外テクニカルニュース：ネローレ雌牛における妊娠喪失と妊娠関連糖タンパク質への雄牛の寄与 Invited
北原 豪
臨床獣医 43 ( 1 ) 72 - 73 2025.1
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：緑書房
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海外テクニカルニュース：反芻動物の生殖補助獣医療における腹腔鏡下採卵技術を用いた体外胚生産 Invited
北原 豪
臨床獣医 42 ( 12 ) 72 - 73 2024.12
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：緑書房
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海外テクニカルニュース：交雑種肉用未経産牛における胞状卵胞数と発情周期の長さとの関係 Invited
北原 豪
臨床獣医 42 ( 11 ) 60 - 61 2024.11
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：緑書房
Presentations 【 display / non-display 】
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大学が関わる新人・新任獣医師への教育
北原 豪
第45回動物臨床医学会年次大会産業動物フォーラム (Web) 日本繁殖生物学会
Event date： 2024.10.6
Language：Japanese Presentation type：Oral presentation (general)
Venue：Web
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経腟採卵(OPU)技術を⽤いたウシにおける卵胞液の酸化ストレス評価の可能性
⾦⽥ 昂⼤、Omnia Elgendy、邉⾒ 広⼀郎、⼩林 郁雄、佐藤 礼⼀郎、⼤澤健司、北原 豪
第15回日本動物超音波技術研究会大会 (Web) 日本繁殖生物学会
Event date： 2024.9.28
Language：Japanese Presentation type：Oral presentation (general)
Venue：Web
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雌牛の分娩前後における腸内と腟内微生物叢の変化ならびに産子の微生物叢との関係
橋口 拓人，Jahidul Islam，古川 睦実，野地 智法、前田 崇史、北原 豪、大澤 健司
第117回日本繁殖生物学会大会 (Web) 日本繁殖生物学会
Event date： 2024.9.22 - 2024.9.25
Language：Japanese Presentation type：Oral presentation (general)
Venue：Web
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牛の難産予測のための妊娠期における胎盤機能および子宮頸管熟化のモニタリング Invited
大澤 健司，北原 豪
第167回日本獣医学会学術集会 (Web) 日本獣医学会
Event date： 2024.9.10 - 2024.9.13
Language：Japanese Presentation type：Oral presentation (general)
Venue：Web
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分娩予定日を過ぎた黒毛和種牛における子宮頸管粘液中のサイトカイン濃度と誘起分娩時の状況との関係
橋口 拓人，上松 瑞穂，金丸 和之，北原 豪、大澤 健司
第167回日本獣医学会学術集会 (Web) 日本獣医学会
Event date： 2024.9.10 - 2024.9.13
Language：Japanese Presentation type：Oral presentation (general)
Venue：Web
Awards 【 display / non-display 】
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第166回日本獣医学会学術集会優秀発表賞
2023.9 日本獣医学会
小東 史織、北原 豪、上松 瑞穂、大澤 健司
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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English Presentation Award
2019.3
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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平成30年度日本獣医師会獣医学術学会年次大会学会長賞
2019.2 日本産業動物獣医学会
鳥巣至道、保坂悠歩、金子泰之、北原豪、日髙勇一、桐野有美、水谷真也、山本集士
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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平成30年度獣医学術九州地区学会産業動物獣医学会地区学会長賞
2018.10 日本産業動物獣医学会九州地区学会
保坂悠歩、鳥巣至道、金子泰之、北原豪、佐藤裕之、水谷真也、山本集士、Benedict Huai Ern ONG、水谷祐子、桐野有美、日髙勇一、浅沼武敏、永延清和
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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平成29年家畜診療賞
2018.2 全国農業共済協会
佐藤知広，北原 豪，大澤健司
Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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牛の子宮頚管熟化機構の解明と分娩後早期の一過性炎症惹起による子宮疾患制御
Grant number：21H02362 2021.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
大澤 健司
Authorship：Coinvestigator(s)
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ウシ潜在性子宮内膜炎の病態解明と治療・予防ストラテジーの確立
Grant number：16H05038 2016.04 - 2020.03
科学研究費補助金 基盤研究(B)
Authorship：Coinvestigator(s)
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GhrelinとNeuromedin U/Sの自律神経様作用の解明と応用への研究
Grant number：16H05043 2016.04 - 2020.03
科学研究費補助金 基盤研究(B)
Authorship：Coinvestigator(s)
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バイオマーカーによる家畜の卵巣機能の質および量的評価法の確立
Grant number：16K18805 2016.04 - 2019.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
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抗ミューラー管ホルモンを用いた新しい繁殖管理技術の開発
Grant number：25850209 2013.04 - 2016.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
これまで国内外で調べられていない雌ウシの春機発動時期と周産期におけるAMHの動態を明らかにし、初産分娩月齢や分娩後の空胎日数などの繁殖効率の指標となる繁殖成績との関係を解析して、AMHをバイオマーカーとした生時より備わっている繁殖能力や周産期における非生産時の繁殖機能を評価する新しい繁殖管理技術の開発に挑む。
Available Technology 【 display / non-display 】
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臨床バイオマーカーを活用した産業動物の繁殖機能評価の開発
効率的な体外胚作出のための培養環境の開発
妊娠期の母牛の栄養状態と胎子・新生子の将来に及ぼす影響に関する研究Related fields where technical consultation is available：産業動物の繁殖機能の評価
胚発生に関わる研究Message：掲げた研究テーマにご興味のある方は、是非、ご連絡ください。