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Affiliation |
Faculty of Agriculture Region of Veterinary Science |
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Professor |
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Related SDGs |
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KITAHARA Go
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Degree 【 display / non-display 】
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博士（獣医学） ( 2012.9 山口大学 )
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学士（獣医学） ( 2002.3 鹿児島大学 )
Research Areas 【 display / non-display 】
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Life Science / Veterinary medical science
Papers 【 display / non-display 】
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Hashiguchi, T., Kitahara, G., Kanemaru, K., Uematsu, M., Osawa, T.
Animal : an international journal of animal bioscience 20 ( 1 ) 101731 2026.1
Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV
During normal cervical ripening in cows, there is an increase in the concentrations of various cytokines in the cervical mucus, which may be a marker of the progression of cervical ripening. However, the cytokine dynamics during abnormal delivery remain unclear. We aimed to clarify the relationship between the concentrations of various cytokines in the cervical mucus of cows past the expected date of calving and the occurrence of dystocia after calving. Cervical mucus was collected from 145 Japanese Black cows with a gestational period of 295days or longer (overdue cows), and calving was induced immediately after collection. Interleukin (IL)-1α, 1β, 6, 8, 10, and tumour necrosis factor (TNF)α concentrations in the mucus were determined. Cytokine concentrations were compared between the eutocia (n=121) and dystocia (n=24) groups. Concentrations of IL-1α, IL-8, and TNFα were lower in the dystocia group than in the eutocia group (P<0.05). To evaluate the accuracy of dystocia prediction, the areas under the receiver operating characteristic curves for IL-1α, IL-8, and TNFα were calculated, with values of 0.638, 0.712, and 0.709, respectively. In conclusion, IL-1α, IL-8 and TNFα concentrations in cervical mucus of overdue cows may be suitable indicators for screening animals at risk of dystocia.
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The effect of heat and cold stress on oxidative stress of follicular microenvironment in beef cattle
KANADA Kodai, ELGENDY Omnia, KOBAYASHI Ikuo, HEMMI Koichiro, SATO Reiichiro, OSAWA Takeshi, KITAHARA Go
Journal of Veterinary Medical Science 87 ( 11 ) 1336 - 1345 2025.9
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE
This study hypothesized that heat and cold stress affect the balance between oxidants and antioxidants (oxidative stress) of oocytes and follicular fluid (FF) in beef cattle and the oxidative stress between blood and FF is correlated. Thirty-two Japanese Black cattle that were divided into four seasonal groups [fall, winter (WG), spring (SpG), and summer (SmG) group]. Plasma and FF were measured diacron-reactive oxygen metabolites (d-ROMs), biological antioxidant potential (BAP), and oxidative stress index (OSI). Reactive oxygen species (ROS) were measured in oocytes. Temperature-humidity index was lower in WG and higher in SmG than in the other three groups (<i>P</i><0.05). d-ROMs value was lower in FF than in plasma in all groups, BAP value was lower in FF than in plasma in WG, SpG, and SmG, and OSI was lower in FF than in plasma in WG and SpG (<i>P</i><0.05). In FF, d-ROMs and BAP values were positively correlated in WG, SpG, and heifer groups (HG, <i>P</i><0.05). d-ROMs or OSI values between plasma and FF were positively correlated in all animals and cow group (CG), SpG, CG, and HG (<i>P</i><0.05). ROS levels in oocytes were higher in WG and SpG than in SmG (<i>P</i><0.05). In conclusion, oxidative stress in the blood and FF are linked, and oxidative stress of oocytes increases in winter and spring in beef cattle that are kept on pasture, except in winter.
DOI： 10.1292/jvms.24-0323
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Dynamics of macrophages and inflammatory cytokine concentrations in the cervix of late pregnant cows Reviewed
Kanemaru K., Kitahara G., Hashiguchi T., Hemmi K., Kobayashi I., Osawa T.
Theriogenology 238 117357 2025.5
Language：English Publishing type：Research paper (scientific journal) Publisher：Theriogenology
Cervical ripening is associated with M1 macrophages and inflammatory cytokines in mice and rats, but its mechanism in cattle is unclear. In this study, to elucidate some of the mechanisms of normal cervical maturation in cows, we examined the expressions of M1 macrophages and mRNA of interleukin (IL)-1α, 1β, 6, 8, and 10, as well as TNFα in cervical tissue and the mRNA expressions and protein concentrations of these cytokines in cervical mucus from late pregnancy to parturition. Forty-one Japanese Black cows were sampled at 200, 230, and 260 d of gestation and then at 7 d intervals until parturition to obtain cervical mucus and tissue samples. The collected tissues were fluorescently immunostained with anti-Iba-1 antibodies, and the macrophage infiltration was checked. Cervical mucus was mixed with PBS, the concentrations of the above cytokines in the supernatant after centrifugation were measured, and the concentration per protein weight was used as the measurement. Total RNA was extracted from the cervical tissue and mucus, and the mRNA expression of various cytokines was analyzed using real-time PCR. In cervical tissue, macrophages stained with anti-Iba-1 antibody were observed from five to six weeks before calving until the week of calving. There was also a trend toward a concurrent increase in the mRNA expression of inflammatory cytokines, especially IL-6, three weeks before calving (P < 0.05). In cervical mucus, the concentrations of IL-1α, IL-1β, IL-8, and TNFα increased (P < 0.05) zero to three weeks before calving compared to 12–14 weeks before calving. In addition, IL-1α mRNA increased three weeks before and during the week of parturition, and IL-8 mRNA increased three weeks before and two weeks after (P < 0.05). These results suggest that cervical ripening in cows begins five to six weeks before calving when macrophages infiltrating cervical tissue may produce large amounts of IL-6, inflammatory cells infiltrate cervical mucus, and IL-1α, IL-1β, IL-8, and TNFα levels in the mucus increase toward calving.
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KOHIGASHI Shiori Saito, UEMATSU Mizuho, KITAHARA Go, OSAWA Takeshi
Journal of Reproduction and Development 71 ( 3 ) 175 - 184 2025.5
Language：English Publishing type：Research paper (scientific journal) Publisher：The Society for Reproduction and Development
This study aimed to clarify the association between the percentage of follicle number by size over antral follicle count (AFC) and subsequent reproductive performance. A total of 306 Japanese Black cattle underwent timed artificial insemination (TAI) 41–62 days postpartum; the AFC and numbers of small, medium, and large follicles were recorded 10 days before TAI. The cross-sectional and blood flow areas of the dominant follicle (DF) on the day of TAI and the corpus luteum (CL) six days after TAI were recorded. The total number of follicles ≥ 2 mm was defined as the AFC, and the percentages of follicle number by each size defined as small (S-AFC%; 2–2.9 mm), medium (M-AFC%; 3–8.4 mm), and large (L-AFC%; ≥ 8.5 mm) follicles. The AFC and S-, M-, and L-AFC% were further grouped into low, medium, and high tertiles, and the subsequent reproductive performance compared among the groups. Plasma anti-Müllerian hormone (AMH) levels were quantified on the day of AFC measurement. No differences were observed in reproductive performance between the AFC and L-AFC% groups. The high-S-AFC% group showed a 20.6% lower conception rate, 0.58 more AI numbers, and 21.9 longer days open than those of the low-S-AFC% group (P < 0.05). The low-M-AFC% group showed an 18.0% lower conception rate after TAI and 0.54 more AI numbers than those of the high-M-AFC% group (P < 0.05). DF and CL parameters did not differ among the AFC, S-, M-, and L-AFC% groups. Plasma AMH levels in the low-AFC group were the lowest in the tertile. In conclusion, the percentage of follicles by size could be used to estimate subsequent reproductive performance.
DOI： 10.1262/jrd.2024-075
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Suzuki, O., Uematsu, M., Kitahara G., Osawa, T., Sasaki, Y.
Reproduction in Domestic Animals 60 ( 4 ) e70058 2025.4
Language：English Publishing type：Research paper (scientific journal) Publisher：Wiley
The calving interval (CI) is an important determinant of reproductive performance in Japanese Black cattle. The present study aimed to compare individual fertility traits among farms classified by CI. In total, 49,780 artificial insemination (AI) records for 7327 cows from 2011 to 2014 were obtained from 622 farms located in Miyazaki, Japan. The farms were classified into three groups (referred to as farm CI groups) using the lower and upper 25 percentiles of farm CI as thresholds. The mean CI of individual cows was 403.5 ± 70.9 days. The interquartile ranges of CI for individual cows were 49 days for short CI farms and 114 days for long CI farms. The interval between calving and the first AI was associated with farm CI group and parity (p < 0.05) but not with calving season and gestation length. The conception rate (CR) at the first AI in cows on long CI farms decreased as the interval from calving to the first AI decreased (p < 0.05). Additionally, no differences in CR among farm CI groups were observed for AI number ≥ 4. In long CI farms, cows with two AI treatments had longer interservice intervals than those for AI values of 3 and ≥ 4 (p < 0.05). In summary, fertility characteristics for long CI farms differed from those of farms with short or middle CI, and standard operating procedures need to be improved to decrease the CI.
Books 【 display / non-display 】
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牛の臨床外科
編著者 佐藤礼一郎（ Role： Contributor）
株式会社緑書房 2025.4
Language：Japanese Book type：Scholarly book
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獣医繁殖学マニュアル
獣医繁殖学教育協議会（ Role： Contributor）
文永堂出版株式会社 2025.3 （ ISBN:978-4-8300-3294-3 ）
Language：Japanese Book type：Scholarly book
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獣医繁殖学第5版
獣医繁殖学教育協議会（ Role： Contributor , 泌乳の生理、生殖器の先天異常、泌乳の異常）
文永堂出版株式会社 2023.9
Language：Japanese Book type：Scholarly book
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牛のマタニティハンドブック
大澤健司・三宅陽一編（ Role： Contributor , P40-45, 54-60）
文永堂出版株式会社 2022.11
Language：Japanese Book type：Scholarly book
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大・中・小動物実験プロトコール
宮崎大学動物実験プロトコール編集委員会（ Role： Joint author）
宮日文化情報センター 2016.3
Language：Japanese Book type：Scholarly book
MISC 【 display / non-display 】
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海外テクニカルニュース：牛の卵胞における異なる発育段階での組織学的あるいはカラードプラ超音波検査による血流解析 Invited
北原 豪
臨床獣医 44 ( 3 ) 64 - 65 2026.3
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：緑書房
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海外テクニカルニュース：肉用未経産牛における早期再同期化にブセレリンとエストラジオールを併用すると受胎率が改善する Invited
北原 豪
臨床獣医 44 ( 2 ) 64 - 65 2026.2
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：緑書房
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海外テクニカルニュース：今後20年間の北米における牛の生産と繁殖に関する展望（後編） Invited
北原 豪
臨床獣医 44 ( 1 ) 66 - 68 2026.1
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：緑書房
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海外テクニカルニュース：今後20年間の北米における牛の生産と繁殖に関する展望（前編） Invited
北原 豪
臨床獣医 43 ( 12 ) 58 - 59 2025.12
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：緑書房
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海外テクニカルニュース：乳房炎は生殖器疾患と同様に繁殖成績に負の影響を及ぼす Invited
北原 豪
臨床獣医 43 ( 11 ) 62 - 63 2025.11
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：緑書房
Presentations 【 display / non-display 】
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黒毛和種牛の繁殖管理 Invited
北原豪
第13回鹿児島産業動物臨床フォーラム (Web) 日本繁殖生物学会
Event date： 2026.2.8
Language：Japanese Presentation type：Public lecture, seminar, tutorial, course, or other speech
Venue：Web
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肉用雄子牛の免疫学的去勢法の検討
西岡快人, 三樹孝太郎, 福家直幸, 大澤健司, 北原豪
令和7年度九州地区日本産業動物獣医学会 (Web) 日本繁殖生物学会
Event date： 2025.10.26
Language：Japanese Presentation type：Oral presentation (general)
Venue：Web
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難産牛の妊娠後期における子宮頸管粘液中サイトカインの動態 International conference
橋口 拓人，陳 禹玄, 前田 崇史，北原 豪，大澤 健司
第118回日本繁殖生物学会大会 (Web) 日本繁殖生物学会
Event date： 2025.9.15 - 2025.9.17
Language：Japanese Presentation type：Oral presentation (general)
Venue：Web
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ウシの母子を護るため、妊娠期に分娩リスクを知る Invited International conference
北原 豪, 前田 崇史, 橋口 拓人, 大澤 健司
第168回日本獣医学会学術集会 (Web) 日本繁殖生物学会
Event date： 2025.9.3 - 2025.9.6
Language：Japanese Presentation type：Symposium, workshop panel (nominated)
Venue：Web
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ウシの分娩に伴う子宮頸管熟化における組織中コラーゲンの動態 International conference
瀬尾 まひな，橋口 拓人，福家 直幸，北原 豪，大澤 健司
第168回日本獣医学会学術集会 (Web) 日本繁殖生物学会
Event date： 2025.9.3 - 2025.9.6
Language：Japanese Presentation type：Oral presentation (general)
Venue：Web
Awards 【 display / non-display 】
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令和7年度獣医学術九州地区学会産業動物獣医学会奨励賞
2025.10 日本産業動物獣医学会九州地区学会
西岡快人, 三樹孝太郎, 福家直幸, 大澤健司, 北原豪
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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第10回伊藤記念財団賞
2025.6 伊藤記念財団
北原豪
Award type：Award from publisher, newspaper, foundation, etc. Country：Japan
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第166回日本獣医学会学術集会優秀発表賞
2023.9 日本獣医学会
小東 史織、北原 豪、上松 瑞穂、大澤 健司
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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English Presentation Award
2019.3
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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平成30年度日本獣医師会獣医学術学会年次大会学会長賞
2019.2 日本産業動物獣医学会
鳥巣至道、保坂悠歩、金子泰之、北原豪、日髙勇一、桐野有美、水谷真也、山本集士
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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牛の子宮頚管熟化機構の解明と分娩後早期の一過性炎症惹起による子宮疾患制御
Grant number：21H02362 2021.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
大澤 健司
Authorship：Coinvestigator(s)
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ウシ潜在性子宮内膜炎の病態解明と治療・予防ストラテジーの確立
Grant number：16H05038 2016.04 - 2020.03
科学研究費補助金 基盤研究(B)
Authorship：Coinvestigator(s)
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GhrelinとNeuromedin U/Sの自律神経様作用の解明と応用への研究
Grant number：16H05043 2016.04 - 2020.03
科学研究費補助金 基盤研究(B)
Authorship：Coinvestigator(s)
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バイオマーカーによる家畜の卵巣機能の質および量的評価法の確立
Grant number：16K18805 2016.04 - 2019.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
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抗ミューラー管ホルモンを用いた新しい繁殖管理技術の開発
Grant number：25850209 2013.04 - 2016.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
これまで国内外で調べられていない雌ウシの春機発動時期と周産期におけるAMHの動態を明らかにし、初産分娩月齢や分娩後の空胎日数などの繁殖効率の指標となる繁殖成績との関係を解析して、AMHをバイオマーカーとした生時より備わっている繁殖能力や周産期における非生産時の繁殖機能を評価する新しい繁殖管理技術の開発に挑む。
Available Technology 【 display / non-display 】
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臨床バイオマーカーを活用した産業動物の繁殖機能評価の開発
効率的な体外胚作出のための培養環境の開発
妊娠期の母牛の栄養状態と胎子・新生子の将来に及ぼす影響に関する研究Related fields where technical consultation is available：産業動物の繁殖機能の評価
胚発生に関わる研究Message：掲げた研究テーマにご興味のある方は、是非、ご連絡ください。