Affiliation |
Faculty of Medicine School of Medicine Department of Infectious Diseases, Parasitology |
Title |
Associate Professor |
External Link |
NAGAYASU Eiji
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Degree 【 display / non-display 】
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博士(医学) ( 2002.3 東海大学 )
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学士(医学) ( 1998.3 宮崎医科大学 )
Papers 【 display / non-display 】
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A surgical case of inflammatory pseudotumor by hepatic anisakiasis. Reviewed
Kotaro Ito, Takao Ide, Tomokazu Tanaka, Eiji Nagayasu, Hideo Hasegawa, Hirokazu Noshiro
Clinical journal of gastroenterology 2023.12
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症例報告 顎口虫による皮膚幼虫移行症の1例
矢野 美有, 田畑 伸子, 瀬川 優里恵, 只木 行啓, 長安 英治, 長谷川 英男
臨床皮膚科 77 ( 9 ) 725 - 729 2023.8
Publishing type:Research paper (scientific journal) Publisher:株式会社医学書院
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Maekawa K., Nagayasu E., Hata Y., Hanamure F., Maruyama H., Yamashita A.
Pathology International 73 ( 8 ) 373 - 376 2023.8
Language:English Publishing type:Case report Publisher:Pathology International
DOI: 10.1111/pin.13352
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Population genetics study of Strongyloides fuelleborni and phylogenetic considerations on primate-infecting species of Strongyloides based on their mitochondrial genome sequences. Reviewed International coauthorship International journal
Phoo Pwint Ko, Misaki Haraguchi, Takashi Hara, Duong Duc Hieu, Ayaka Ito, Ryusei Tanaka, Mio Tanaka, Takafumi Suzumura, Miya Ueda, Ayako Yoshida, Haruhiko Maruyama, Eiji Nagayasu
Parasitology international 92 102663 - 102663 2023.2
Authorship:Last author, Corresponding author Language:English Publishing type:Research paper (scientific journal)
Strongyloides is a genus of parasitic nematodes of vertebrates comprising approximately 50 documented species, each with various host ranges. Among these, three species (S. stercoralis, S. fuelleborni, and S. cebus) are known to infect primate hosts. S. fuelleborni typically infects non-human primates in the Old World. To complement the existing information on the global genetic structure of this species, we conducted a genotyping study of S. fuelleborni samples collected from rhesus macaques in Myanmar, Japanese macaques in Japan, and some zoo-kept primates. This study identified a novel haplotype group in isolates from the Myanmar rhesus macaques. Subsequently, we obtained the complete or nearly complete mitochondrial genome sequences of S. fuelleborni, S. cebus (Strongyloides of New World monkeys), and S. vituli (Strongyloides of cattle). Phylogenetic analysis based on concatenated mitochondrial protein sequences of various Strongyloides species indicated a close relationship between S. fuelleborni, S. vituli and S. papillosus (Strongyloides in sheep and cattle). S. cebus is quite distantly related to both S. fuelleborni and S. stercoralis, which led to the hypothesis that the three primate Strongyloides species evolved independently as parasites of primates.
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Diagnostic value of recombinant nanoluciferase fused Toxoplasma gondii antigens in Luciferase-linked Antibody Capture Assay (LACA) for Toxoplasma infection in pigs. Reviewed International coauthorship
Hieu Duc Duong, Yuji Taniguchi, Yasuhiro Takashima, Satoshi Sekiguchi, Khin Myo Aye, Parnian Ahmadi, Linh Khanh Bui, Takao Irie, Eiji Nagayasu, Ayako Yoshida
The Journal of veterinary medical science 84 ( 7 ) 905 - 913 2022.7
Language:English Publishing type:Research paper (scientific journal) Publisher:JAPANESE SOCIETY OF VETERINARY SCIENCE
Toxoplasmosis is a widespread protozoan zoonosis. Since ingesting undercooked meat harboring Toxoplasma gondii cyst is considered one of the major transmission routes to humans, the screening of T. gondii in meat-producing animals can reduce the risk of food-borne toxoplasmosis in humans. Among serological diagnostic methods, Luciferase-linked Antibody Capture Assay (LACA) has been found to be a promising platform with high sensitivity and specificity. In this study, we aimed to evaluate recombinant nanoluciferase fused-T. gondii antigens (rNluc-GRA6, rNluc-GRA7, rNluc-GRA8 and rNluc-BAG1) for their potential use in LACA for pigs. As a result, the sensitivity of GRA6-, GRA7-, GRA8- and BAG1-LACA were 70.0%, 80.0%, 80.0% and 30.0% with specificity 87.0%, 81.5%, 74.1% and 50.0%, respectively. The cocktail LACA using a mixture of rNluc-GRA6, rNluc-GRA7 and rNluc-GRA8 indicated higher sensitivity (90.0%) and a similar specificity (96.3%) in comparison with the commercial ELISA kit. Compared to the Dye-Test as a reference test, cocktail LACA showed strong agreement (kappa value=0.811) when we assessed pig sera collected at the slaughterhouse. In addition, we also successfully established the rapid LACA format for the detection of Toxoplasma infection in pigs (called Rapid-LACA) in which the test could be performed within 30 min. In Rapid-LACA, the protein A pre-coated/blocked plates could be preserved at -30°C, 4°C or room temperature conditions for at least two months without compromising on the quality of assay.
DOI: 10.1292/jvms.22-0099
MISC 【 display / non-display 】
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ヒトとイヌに由来するStrongyloides stercoralisの分子系統解析
29 ( 1 ) 21 - 23 2018.12
Language:Japanese Publishing type:Article, review, commentary, editorial, etc. (scientific journal)
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【多様化する感染症研究】 熱帯寄生虫症に対する集団投薬の最適化の必要性(解説/特集) Invited
長安 英治
細胞 49 ( 14 ) 704 - 707 2017.12
Language:Japanese Publishing type:Article, review, commentary, editorial, etc. (scientific journal)
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【寄生虫症(II)】 糞線虫症研究の新展開(解説/特集) Invited
長安 英治, 菊池 泰生, 丸山 治彦
医学と薬学 74 ( 11 ) 1407 - 1414 2017.10
Language:Japanese Publishing type:Article, review, commentary, editorial, etc. (scientific journal)
Presentations 【 display / non-display 】
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A Case of Sparganosis Mansoni
Hiromi Narita, Yujiro Asada, Eiji Nagayasu, Haruhiko Maruyama
The Japanese Dermatological Association 164th Miyazaki regional meeting
Event date: 2024.4.6 - 2024.4.7
Language:Japanese Presentation type:Oral presentation (general)
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ペルーのヒトおよびイヌ由来糞線虫の分子系統解析
森下和広, 丸山治彦
第 72 回日本寄生虫学会南日本支部大会
Event date: 2019.10.26
Language:Japanese Presentation type:Oral presentation (general)
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ヒトの糞線虫はどこから来たのか-ネコ目由来糞線虫との関係から
長安英治, 丸山治彦
第12回蠕虫研究会 蠕虫研究会
Event date: 2018.11.17 - 2018.11.18
Language:Japanese Presentation type:Oral presentation (general)
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Genetic relationship of Ancylostoma ceylanicum isolated from dogs and humans in Vietnam inferred from mt cox1 gene
Duc Hieu Duong, Khanh Linh Bui, Thu Huong Nguyen, Thanh Duong Tran, 長安 英治, 丸山 治彦, 吉田 彩子, 野中 成晃
第161回日本獣医学会学術集会 (つくば市)
Event date: 2018.9.11 - 2018.9.13
Language:English Presentation type:Oral presentation (general)
Venue:つくば市
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ゴリムマブ治療中に重症糞線虫感染と診断したHTLV-1抗体陽性関節リウマチの1例
橋場弥生, 南寛之, 河野敦子, 宮田義史, 日高利彦, 長安英治, 丸山治彦, 梅北邦彦, 岡山昭彦
第5回日本HTLV-1学会学術集会
Event date: 2018.8.31 - 2018.9.2
Language:Japanese Presentation type:Poster presentation
Grant-in-Aid for Scientific Research 【 display / non-display 】
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寄生虫の分子同定と系統解析のための統一標準法の確立
Grant number:21H02725 2021.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
丸山 治彦、長安 英治
Authorship:Coinvestigator(s)
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ヒト糞線虫の起源:アジア、アフリカ、南米の霊長類を中心としたサンプリングから
Grant number:20K07464 2020.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship:Principal investigator
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ヒトとイヌに感染する糞線虫の種の歴史:進化的起源・分化・拡散に関する研究
2017.04 - 2020.03
科学研究費補助金 基盤研究(C)
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動物寄生線虫(糞線虫)の宿主環境知覚に伴う発育再開メカニズムの解明
2014.04 - 2017.03
科学研究費補助金 基盤研究(C)
Authorship:Principal investigator
動物寄生線虫(糞線虫)の宿主環境知覚に伴う発育再開メカニズムの解明
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動物寄生線虫の持つ細菌様フェロケラターゼ(鉄挿入酵素)の解析
2011.04 - 2014.03
科学研究費補助金 若手研究(B)
Authorship:Principal investigator
動物寄生線虫の持つ細菌様フェロケラターゼ(鉄挿入酵素)の解析
Other research activities 【 display / non-display 】
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ミャンマーにおける糞線虫症フィールド調査
2015.01
ミャンマーにおける糞線虫症のフィールド調査
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ウガンダにおける寄生虫症フィールドワーク
2014.10 - 2014.11
ウガンダにおけるフィラリア症の流行状況の調査
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ウガンダにおける寄生虫症フィールドワーク
2014.05
ウガンダにおけるマラリア感染者及び非感染者における腸内細菌叢の解析
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ミャンマーにおける寄生虫症共同研究
2014.01 - 2014.02
University of Medicine (1), Yangonにおける寄生虫症に関するセミナー及び共同研究の実施
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ウガンダにおける寄生虫症フィールドワーク
2013.10 - 2013.11
Gulu大学との国際共同研究