|
Affiliation |
Frontier Science Research Center Experiment support section inheritance resource field |
|
Title |
Assistant Professor |
|
External Link |
|
GONDO Takahiro
|
|
|
Research Areas 【 display / non-display 】
-
Life Science / Animal production science
-
Environmental Science/Agriculture Science / Science in plant genetics and breeding
Papers 【 display / non-display 】
-
Quantitative analysis of seven plant hormones in Lotus japonicus using standard addition method
Hashiguchi T., Hashiguchi M., Tanaka H., Fukushima K., Gondo T., Akashi R.
PLoS ONE 16 ( 2 February ) e0247276 2021.2
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：PLoS ONE
Plant hormones have been identified to be versatile signaling molecules essential for plant growth, development, and stress response. Their content levels vary depending on the species, and they also change in response to any external stimuli. Thus, simultaneous quantification of multiple plant hormones is required to understand plant physiology. Sensitive and quantitative analysis using liquid chromatography-linked mass spectrometry (LC-MS/MS) has been used in detecting plant hormones; however, quantification without stable isotopes is yet to be established. In this study, we quantified seven representative plant hormones of Lotus japonicus, which is a model legume for standard addition method. Accurate masses for monoisotopic ions of seven phytohormones were determined for high-resolution mass spectrometry (HR-MS). Selected ion monitoring (SIM) mode based on accurate masses was used in detecting phytohormones in the roots, stems, and leaves. Evaluation of matrix effects showed ion suppression ranging from 10.2% to 87.3%. Both stable isotope dilution and standard addition methods were able to detect plant hormones in the roots, stems, and leaves, with no significant differences in using both approaches and thus a standard addition method can be used to quantify phytohormones in L. japonicus. The method will be effective, especially when stable isotopes are not available to correct for matrix effects.
-
Comparative analysis of seed proteome of Glycine max and Glycine soja Reviewed
Hashiguchi T., Hashiguchi M., Tanaka H., Gondo T., Akashi R.
Crop Science 60 ( 3 ) 1530 - 1540 2020.5
Language：English Publishing type：Research paper (scientific journal) Publisher：Crop Science
© 2020 The Authors. Crop Science © 2020 Crop Science Society of America Soybean, a globally important grain legume, contains large amounts of proteins, lipids, and secondary metabolites and, when ingested, has a wide array of physiological functions that may improve human health. In the development of cultivated soybean [Glycine max (L.) Merr.] with improved abiotic and biotic stress resistance, high yield, and seed quality, wild soybean (Glycine soja Siebold & Zucc.) has been an excellent source of germplasm, owing to its genetic diversity. Although genomic differences between the two species have been intensively studied, the differences in seed protein expression remain poorly understood. In the present study, we used a shotgun proteomic comparative analysis approach to further characterize the seed proteome in cultivated and wild soybean. Protamine sulfate-mediated precipitation successfully decreased the levels of two major proteins that had previously masked the detection of low-abundant proteins. We identified 65 differentially expressed proteins between the two species, with 39 proteins expressed more highly in G. soja and 26 proteins expressed more highly in G. max. Among these proteins, various stress tolerance-related proteins were found in wild soybean, perhaps reflecting its adaptation to diverse ecological conditions. Cultivated soybean expressed high levels of sucrose and lipid biosynthesis proteins, and this was also confirmed at the transcriptional level. Bioinformatic analyses revealed that cellular and metabolic process was the functional category most frequently linked to the differentially expressed proteins. These data provide valuable information that will contribute to a better understanding of soybean seed biology and help to promote G. max breeding for improved agronomic traits using wild relatives.
DOI： 10.1002/csc2.20131
-
Oxalate and silica contents of seven varieties of napier grass (Pennisetum purpureum) Reviewed
Rahman M.M., Norshazwani M.S., Gondo T., Maryana M.N., Akashi R.
South African Journal of Animal Sciences 50 ( 3 ) 397 - 402 2020
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：South African Journal of Animal Sciences
© 2020, South African Bureau for Scientific Publications. Oxalate and silica are considered antinutrients. Large quantities of oxalate and silica in plants can interfere with the uptake of essential minerals in ruminants. Therefore, the aim of this study was to compare the total silica and oxalate contents of seven varieties of Napier grass to find out which is best for cultivation. Taiwan, Zanzibar, Pakchong, Purple, Kobe, Indian, and Dwarf Napier grass were grown in a completely randomized design with three replications to determine their soluble oxalate, total oxalate, and silica contents. Plants were harvested at two months of plant maturity. Whole plant of the Dwarf Napier grass contained significantly higher soluble oxalate content than tall varieties. Total oxalate content in whole plant differed significantly among varieties. Dwarf showed the highest total oxalate content (3.23% dry matter (DM)) followed by Kobe (2.61%), Zanzibar (2.60%), Purple (2.44%), Taiwan (2.43%), Indian (2.15%), and Pakchong (1.95%). Regardless of variety, leaf tissue contained significantly higher soluble oxalate and total oxalate than stem tissue. There were no differences in silica content among them. In conclusion, the tall varieties could produce lower levels of soluble oxalate than the Dwarf variety, whereas silica content might not vary among them.
-
Rahman M.M., Ismail S.N.B., Mat K., Gondo T., Nor M.M., Akashi R.
Pertanika Journal of Tropical Agricultural Science 43 ( 4 ) 609 - 619 2020
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Pertanika Journal of Tropical Agricultural Science
The beneficial effects of feeding rabbits with forages have been recommended. However, limited study has been made on feeding values of locally available forages in rabbits. Therefore, an experiment was conducted to evaluate the effect of Asyastasia gangetica and Brachiaria decunbens on intake, digestibility, and growth performance of rabbits. Twelve rabbits were distributed into three diets: (i) 100% commercial pellet as control (T1), (ii) 50% pellet plus Asyastasia gangetica ad libitum (T2), and (iii) 50% pellet plus Brachiaria decumbens ad libitum (T3). Daily feed intake, nutrient digestibility, weekly body weight, and feed conversion ratio (FCR) were measured. Intakes of total dry matter (DM) (121.2-134.3 g/d) and organic matter (OM) (115.1-132.5 g/d) were similar (p>0.05) for all the groups. The crude protein (CP) intake of rabbits fed with T1 (10.1 g/d) and T3 (9.6 g/d) diets was similar (p>0.05), but lower (p<0.05) than T2 (14.1 g/d) diet. Ether extract intake of rabbits fed with T2 (2.9 g/d) and T3 (3.9 g/d) diets was similar (p>0.05), but lower (p<0.05) than T1 (5.1 g/d) diet. Neutral detergent fibre (NDF) intake was higher (p<0.05) for rabbits fed with T3 (55.8 g/d) diet followed by T2 (41.7 g/d) and T1 (31.7 g/d) diets. There were no differences (p>0.05) on the digestibilities of DM, OM, and CP among treatments. Total weight gain and daily weight gain were higher (p<0.05) for rabbits fed with T1 (568 and 11.0 g) and T2 (468 and 9.0 g) diets than T3 (155 and 3.3 g) diet, respectively. The lowest FCR was obtained with T1 (12.3) and T2 (13.9) diets, whereas the highest was obtained with T3 (30.3) diet. In conclusion, diet containing Asyastasia gangetica showed more benefits in terms of CP and NDF intakes, weight gain and FCR than the diet containing Brachiaria decumbens. A combination of concentrate and Asyastasia gangetica is recommended as a partial replacement for concentrate in rabbit production.
-
Arifin A., Gondo T., Akashi R.
Plant Biotechnology 36 ( 4 ) 233 - 240 2019.12
Language：English Publishing type：Research paper (scientific journal) Publisher：Plant Biotechnology
© 2019 The Japanese Society for Plant Cell and Molecular Biology. The application of useful genes from model plants to crops is an important step to verify its agricultural usefulness. SYNC1, an asparaginyl-tRNA synthetase gene, was previously identified through the Full-length cDNA Over-eXpressor gene (FOX gene-hunting system) of Arabidopsis cDNA by using super-growing root (SR) culture of Lotus corniculatus, and was suggested to have a potential in increasing some amino acid contents and plant biomass. To identify the functionality of SYNC1 gene in a typical legume crop soybean, the effects of its overexpression in transgenic plants to agricultural traits and free amino acid contents were evaluated. The transgenic soybean plants were produced from infected half-seed explants of 1 day old seedlings with the suspension of Agrobacterium tumefaciens harboring expression vector pB7WG2D-SYNC1. The transgenic plants that overexpressed SYNC1 gene had increased asparagine and lysine contents in matured seeds, and increased aspartate, lysine, alanine and histidine contents in germinated seeds. The changes in those free amino acid contents affected plant morphology and led to significant increase in plant length, number of branches and number of branch nodes as yield components of soybean. The transgenic plants also showed a tendency of higher number of pods, seeds and total seed weight per plant. These results showed that the overexpression of SYNC1 gene contributes on the increase of plant free amino acid contents and biomass, and this approach is expected to be applicable in other legumes, grain and forage crops.
Books 【 display / non-display 】
-
形質転換プロトコール（植物編）「2章2-3バヒアグラス」
権藤 崇裕、明石 良（ Role： Contributor）
化学同人 2012.9
Total pages：412 Responsible for pages：97-105 Language：Japanese Book type：Scholarly book
MISC 【 display / non-display 】
-
暖地型イネ科牧草類のバイオ技術 ―組織培養・遺伝子組換え技術の確立からゲノム編集技術の開発まで― Invited
権藤 崇裕
アグリバイオ 3 ( 10 ) 975 - 980 2019.9
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
-
バイオリソース＆データベース活用術〜ミヤコグサ・ダイズ モデル植物から作物への展開 Invited
橋口正嗣、権藤崇裕、明石 良
細胞工学 別冊 212 - 215 2009.7
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
-
AKASHI Ryo, GONDO Takahiro, KAWAMURA Osamu
Japanese Journal of Grassland Science 49 ( 1 ) 79 - 87 2003
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Japanese Society of Grassland Science
DOI： 10.14941/grass.49.79
Presentations 【 display / non-display 】
-
2017年中に発表されたゲノム編集イネのオフターゲット効果に関する評価
成島純平、中村公亮 他
日本農芸化学会大会
Event date： 2020.3.25 - 2020.3.28
Language：Japanese Presentation type：Oral presentation (general)
-
バヒアグラスにおけるリグニン生合成に関わるCAD遺伝子のゲノム編集
権藤崇裕、湯浅玲奈、明石良
第76回日本草地学会
Event date： 2020
Language：Japanese Presentation type：Oral presentation (general)
-
Asparaginyl-tRNA synthetase gene (SYNC1) characterized by lotus corniculatus FOX-superroot lines has effects on plant morphology and amino acid contents of seed in soybean
Azeri Gautama Arifin, Takahiro Gondo, Ryo Akashi
第37回日本植物細胞分子生物学会
Event date： 2019.9.7 - 2019.9.8
Language：English Presentation type：Oral presentation (general)
-
バヒアグラス遺伝子組換え体におけるCAD遺伝子の発現抑制がPAL、COMT遺伝子の発現とリグニン含量に及ぼす影響
権藤崇裕、山田智仁、湯浅玲奈、明石良
日本草地学会大会
Event date： 2019
Language：Japanese Presentation type：Oral presentation (general)
-
コウシュンシバにおけるアグロバクテリウム法による遺伝子組換え系の確立
牛山真里、張震、明石良、権藤崇裕
第36回日本植物細胞分子生物学会
Event date： 2019
Language：Japanese Presentation type：Poster presentation
Grant-in-Aid for Scientific Research 【 display / non-display 】
-
植物の遺伝子組換え技術について学ぼう！
2019
科学研究費補助金 研究成果公開促進費・研究成果公開発表(B)
Authorship：Principal investigator
-
植物の遺伝子組換え技術について学ぼう！
2018
科学研究費補助金 研究成果公開促進費・研究成果公開発表(B)
Authorship：Principal investigator
-
牧草・芝草におけるゲノム編集による新たな育種技術の基盤構築
2017 - 2020.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
-
植物の遺伝子組換え技術について学ぼう！
2017
科学研究費補助金 研究成果公開促進費・研究成果公開発表(B)
Authorship：Principal investigator
-
植物の遺伝子組換え技術について学ぼう！
2016
科学研究費補助金 研究成果公開促進費・研究成果公開発表(B)
Authorship：Principal investigator