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Frontier Science Research Center Department of Biological Science Division of Biofunction Analysis Kibana Section |
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Assistant Professor |
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Related SDGs |
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GONDO Takahiro
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Research Areas 【 display / non-display 】
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Life Science / Animal production science
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Environmental Science/Agriculture Science / Science in plant genetics and breeding
Papers 【 display / non-display 】
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Prasetyo B., Suwignyo B., Ishigaki G., Gondo T., Jati D.K., Prasojo Y.S.
BIO Web of Conferences 164 2025.3
Publishing type：Research paper (scientific journal) Publisher：BIO Web of Conferences
This study aims to determine the effect of harvesting at different growth stages on nutrient content of jack bean plants cultivated in alluvial type of soil at Blora, Central Java, Indonesia. A field experiment was conducted using a completely randomized block design with four replications. Plant in every plot were randomly selected and hand separated. Each component was sampled approximately 1000 g. Data were analyzed using Analysis of Variance (ANOVA) and continued with Duncan’s Multiple Range Test (p<0.05). The following parameter observed were nutrient content include dry matter (DM), organic matter (OM), crude protein (CP), crude fiber (CF), dan ether extract (EE). The result showed that growth stages had a significant effect (P<0,05) on DM and CF, very significantly (P<0,01) on OM and CP, while the CF and EE not significantly (P>0,05). DM and CF content increased during the flowering growth stage. On the other hand, the OM content increased as the growth stage increased. However, the CP and EE content decreased as growth stages increased. The vegetative stage is considered the best harvesting stage because it has the highest crude protein content, as leguminous plants are forage plants and an important source of protein for ruminant feed.
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Arifin A.G., Shimamoto Y., Tanaka H., Hashiguchi M., Akashi R., Gondo T.
Legume Research 48 ( 1 ) 57 - 63 2025.1
Authorship：Corresponding author Publishing type：Research paper (scientific journal) Publisher：Legume Research
Background: Identifying biomass-related genes to meet the increasing demand for food supply is important approach for agricultural and scientific efforts. In this study, we employed the Full-length cDNA Over-eXpressing gene hunting system (FOX hunting system) to identify biomass-related genes through ectopic expression of Arabidopsis thaliana full-length cDNA in bird’s-foot trefoil (Lotus corniculatus). Methods: A total of 66 FOX superroot lines (FSLs) from Lotus corniculatus cv. Viking were transferred in vermiculite after two weeks in half-strength Murashige and Skoog (MS) medium. These FSLs were then grown in a growth chamber for four weeks, during which their plant length and root length were measured. High biomass lines were selected from this group. FSL #83 and #121 were then grown for an additional four months in a greenhouse until they reached maturity, at which point they were evaluated for their agronomic traits. Result: Out of the 66 analyzed FSLs, 15 displayed superior growth characteristics in plant length and total root length. Arabidopsis cDNA, categorized into genes related to various functions such as membrane transport, photosystems, phytohormone synthesis, amino acid synthesis and unknown genes, was introduced into these lines. Two lines were grown to maturity in the greenhouse and their agronomic traits were analyzed in detail. FSL #83 expressed brassinosteroid biosynthetic pathway gene (CPD) led to significant differences in internode length, number of stems, number of flowers and above-ground dry matter weight compared to non-transgenic Super-growing roots (SR). On the other hand, FSL #121 expressed Asparaginyl-tRNA synthetase (SYNC1) gene exhibited increases in plant length and the number of stem nodes.
DOI： 10.18805/LRF-813
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Nakamura K., Gondo T., Chiba S., Akimoto S., Narushima J., Kondo K., Sugano Y., Tanaka H., Hashiguchi M., Shiwa Y., Akashi R.
Agriculture (Switzerland) 14 ( 12 ) 2024.12
Publishing type：Research paper (scientific journal) Publisher：Agriculture (Switzerland)
Gene expression in individual germinated seeds of four different soybean (Glycine max [L.] Merr.) cultivars, GL3494, OAC Kent, Williams 82, and Jackson, was examined using single seed high-throughput RNA sequencing (ssRNA-seq). The gene expression was similar between two individual seeds of the same cultivar, but different among individual seeds of the four cultivars. Notably, ssRNA-seq identified five genes that were not stably expressed in Williams 82, having either no detectable sequence reads or less than five sequence reads mapped to the exon regions of chromosomes in Williams 82. These findings were validated by reverse transcription polymerase chain reaction analysis. The study’s results demonstrate that gene expression in germinated seeds is unique to an individual seed from each soybean cultivar. This uniqueness may affect composition and content of nutrients in germinated soybeans used as food ingredients.
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宮崎大学フロンティア科学総合研究センター・遺伝資源分野 Reviewed
権藤 崇裕, 橋口 正嗣
生物工学会誌 102 ( 10 ) 529 - 530 2024.10
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：公益社団法人 日本生物工学会
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Ng H.M., Gondo T., Tanaka H., Akashi R.
Plant Cell Reports 43 ( 2 ) 50 2024.2
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Plant Cell Reports
Key Message: Genome editing by CRISPR/Cas9 can be applied to Z. matrella ‘Wakaba’, and knockout mutants of ZmNYC1 gene exhibited stay-green phenotype and reduced tillering. Abstract: Zoysia matrella is a widely used C4 warm-season turfgrass for landscaping, golf courses, and sports fields. Here, we used the CRISPR/Cas9 system to target the Non-Yellow Coloring1 (ZmNYC1) gene in the highly heterozygous allotetraploid Z. matrella ‘Wakaba’, aiming to generate a novel stay-green variety. Of 441 Agrobacterium-infected calli, 22 (5.0%) were transformed, and 14 of these (63.6%) showed targeted mutations through cleaved amplified polymorphic sequences analysis. Sequencing analysis revealed mutations mostly consisting of 1 or 2 bp indels, occurring 2 to 4 bp upstream of the PAM sequence. Regenerated plants exhibited five ZmNYC1 target locus genotypes, including homozygous mutants with a complete knockout of all four alleles in the T0 generation. Under dark treatment, ZmNYC1-mutated plants displayed suppressed chlorophyll b (Chl b) degradation, leading to higher chlorophyll content and Chl b, with a lower chlorophyll a/chlorophyll b ratio compared to the wild type (WT). However, the ZmNYC1 mutation also inhibited plant growth in homozygous mutant genotypes, exhibiting reduced tillering compared to WT. Additionally, during winter simulation, mutant with a complete knockout retained greenness longer than the WT. This is the first successful use of CRISPR/Cas9 genome editing in zoysiagrass. The mutants of the ZmNYC1 gene would serve as valuable breeding material for developing improved zoysiagrass varieties that can maintain their green color for longer periods, even during winter dormancy.
Books 【 display / non-display 】
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草類遺伝資源の保全と活用
日本草地学会（ Role： Joint author , 第11章 バイオテクノロジーによる暖地型イネ科牧草の育種）
養賢堂 2024.7
Language：Japanese Book type：Scholarly book
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植物バイオテクノロジー入門 組織培養からゲノム編集まで
田部井 豊 他（ Role： Joint author , ローズグラスの組織培養および形質転換）
国際文献社 2022.3 （ ISBN:978-4-910603-03-2 ）
Book type：Scholarly book
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形質転換プロトコール（植物編）「2章2-3バヒアグラス」
権藤 崇裕、明石 良（ Role： Contributor）
化学同人 2012.9
Total pages：412 Responsible for pages：97-105 Language：Japanese Book type：Scholarly book
MISC 【 display / non-display 】
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暖地型イネ科牧草類のバイオ技術 ―組織培養・遺伝子組換え技術の確立からゲノム編集技術の開発まで― Invited
権藤 崇裕
アグリバイオ 3 ( 10 ) 975 - 980 2019.9
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
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バイオリソース＆データベース活用術〜ミヤコグサ・ダイズ モデル植物から作物への展開 Invited
橋口正嗣、権藤崇裕、明石 良
細胞工学 別冊 212 - 215 2009.7
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
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AKASHI Ryo, GONDO Takahiro, KAWAMURA Osamu
Japanese Journal of Grassland Science 49 ( 1 ) 79 - 87 2003
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Japanese Society of Grassland Science
DOI： 10.14941/grass.49.79
Presentations 【 display / non-display 】
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牧草の葉の老化に伴うSGR遺伝子の発現およびクロロフィル分解とタンパク質含量との関係
瀬戸口碧希、権藤崇裕
2025年度日本草地学会
Event date： 2025.3.17 - 2025.3.19
Language：Japanese Presentation type：Poster presentation
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バヒアグラスにおけるリグニン生合成に関わるCAD遺伝子のゲノム編集 − CAD変異遺伝子座のF2分離集団における形態および繊維成分の評価 –
権藤崇裕、綿島彩華、池亀麗華
2024年度日本育種学会講演会
Event date： 2024.9.19 - 2024.9.20
Language：Japanese Presentation type：Oral presentation (general)
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タイヌビエのカルスの性状と効率的な組織培養系の確⽴
権藤崇裕、菅原まなみ、岩上哲史
2023年度日本育種学会講演会
Event date： 2023.9.16 - 2023.9.18
Language：Japanese Presentation type：Poster presentation
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ゲノム編集による常緑シバ新規育種素材の開発：NYC1 遺伝子をノックアウトしたコウシュンシバの作出と評価
ホアン ワンメイ、平田 真里菜、権藤 崇裕、明石 良
2023年度日本育種学会講演会
Event date： 2023.9.16 - 2023.9.18
Language：Japanese Presentation type：Poster presentation
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バヒアグラスにおけるリグニン生合成に関わるCAD遺伝子のゲノム編集 − CAD変異遺伝子座のホモ集団の作出とその評価 −
綿島彩華、権藤崇裕、明石 良
2023年度日本育種学会講演会
Event date： 2023.9.16 - 2023.9.18
Language：Japanese Presentation type：Oral presentation (general)
Grant-in-Aid for Scientific Research 【 display / non-display 】
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タイヌビエ多剤抵抗性発現の鍵となる除草剤代謝遺伝子群の一斉発現システムの解明
Grant number：23K23612 2024.04 - 2026.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(B)
Authorship：Coinvestigator(s)
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ゲノム編集による高消化性暖地型イネ科牧草の作出とその遺伝子拡散リスク評価
Grant number：23K05524 2023.04 - 2027.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
Authorship：Principal investigator
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タイヌビエ多剤抵抗性発現の鍵となる除草剤代謝遺伝子群の一斉発現システムの解明
Grant number：22H02347 2022.04 - 2026.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
Authorship：Coinvestigator(s)
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植物の遺伝子組換え技術について学ぼう！
Grant number：99999999 2019
科学研究費補助金 研究成果公開促進費・研究成果公開発表(B)
Authorship：Principal investigator
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植物の遺伝子組換え技術について学ぼう！
Grant number：99999999 2018
科学研究費補助金 研究成果公開促進費・研究成果公開発表(B)
Authorship：Principal investigator
Available Technology 【 display / non-display 】
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暖地型イネ科牧草の品質向上に関する研究
ダイズの収量性向上に関する研究
遺伝子組換え作物における生物多様性影響評価Home Page： 草類遺伝資源・育種学研究室
Related fields where technical consultation is available：・植物の組織培養および遺伝子組換え
・遺伝子組換え作物の生物多様性影響評価Message：・共同研究の希望テーマ：植物の遺伝子組換えと生物多様性影響評価
・「植物の大量増殖および遺伝子組み換え作物の評価」というニーズがあれば、ぜひ教えてください。