権藤 崇裕 (ゴンドウ タカヒロ)

GONDO Takahiro

写真a

所属

フロンティア科学総合研究センター 実験支援部門遺伝資源分野

職名

助教

外部リンク

学位 【 表示 / 非表示

  • 博士(農学) ( 2004年3月   鹿児島大学 )

研究分野 【 表示 / 非表示

  • ライフサイエンス / 動物生産科学

  • 環境・農学 / 遺伝育種科学

 

論文 【 表示 / 非表示

  • Quantitative analysis of seven plant hormones in Lotus japonicus using standard addition method

    Hashiguchi T., Hashiguchi M., Tanaka H., Fukushima K., Gondo T., Akashi R.

    PLoS ONE   16 ( 2 February )   e0247276   2021年2月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PLoS ONE  

    Plant hormones have been identified to be versatile signaling molecules essential for plant growth, development, and stress response. Their content levels vary depending on the species, and they also change in response to any external stimuli. Thus, simultaneous quantification of multiple plant hormones is required to understand plant physiology. Sensitive and quantitative analysis using liquid chromatography-linked mass spectrometry (LC-MS/MS) has been used in detecting plant hormones; however, quantification without stable isotopes is yet to be established. In this study, we quantified seven representative plant hormones of Lotus japonicus, which is a model legume for standard addition method. Accurate masses for monoisotopic ions of seven phytohormones were determined for high-resolution mass spectrometry (HR-MS). Selected ion monitoring (SIM) mode based on accurate masses was used in detecting phytohormones in the roots, stems, and leaves. Evaluation of matrix effects showed ion suppression ranging from 10.2% to 87.3%. Both stable isotope dilution and standard addition methods were able to detect plant hormones in the roots, stems, and leaves, with no significant differences in using both approaches and thus a standard addition method can be used to quantify phytohormones in L. japonicus. The method will be effective, especially when stable isotopes are not available to correct for matrix effects.

    DOI: 10.1371/journal.pone.0247276

    Scopus

    PubMed

  • Comparative analysis of seed proteome of Glycine max and Glycine soja 査読あり

    Hashiguchi T., Hashiguchi M., Tanaka H., Gondo T., Akashi R.

    Crop Science   60 ( 3 )   1530 - 1540   2020年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Crop Science  

    © 2020 The Authors. Crop Science © 2020 Crop Science Society of America Soybean, a globally important grain legume, contains large amounts of proteins, lipids, and secondary metabolites and, when ingested, has a wide array of physiological functions that may improve human health. In the development of cultivated soybean [Glycine max (L.) Merr.] with improved abiotic and biotic stress resistance, high yield, and seed quality, wild soybean (Glycine soja Siebold & Zucc.) has been an excellent source of germplasm, owing to its genetic diversity. Although genomic differences between the two species have been intensively studied, the differences in seed protein expression remain poorly understood. In the present study, we used a shotgun proteomic comparative analysis approach to further characterize the seed proteome in cultivated and wild soybean. Protamine sulfate-mediated precipitation successfully decreased the levels of two major proteins that had previously masked the detection of low-abundant proteins. We identified 65 differentially expressed proteins between the two species, with 39 proteins expressed more highly in G. soja and 26 proteins expressed more highly in G. max. Among these proteins, various stress tolerance-related proteins were found in wild soybean, perhaps reflecting its adaptation to diverse ecological conditions. Cultivated soybean expressed high levels of sucrose and lipid biosynthesis proteins, and this was also confirmed at the transcriptional level. Bioinformatic analyses revealed that cellular and metabolic process was the functional category most frequently linked to the differentially expressed proteins. These data provide valuable information that will contribute to a better understanding of soybean seed biology and help to promote G. max breeding for improved agronomic traits using wild relatives.

    DOI: 10.1002/csc2.20131

    Scopus

  • Oxalate and silica contents of seven varieties of napier grass (Pennisetum purpureum) 査読あり

    Rahman M.M., Norshazwani M.S., Gondo T., Maryana M.N., Akashi R.

    South African Journal of Animal Sciences   50 ( 3 )   397 - 402   2020年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:South African Journal of Animal Sciences  

    © 2020, South African Bureau for Scientific Publications. Oxalate and silica are considered antinutrients. Large quantities of oxalate and silica in plants can interfere with the uptake of essential minerals in ruminants. Therefore, the aim of this study was to compare the total silica and oxalate contents of seven varieties of Napier grass to find out which is best for cultivation. Taiwan, Zanzibar, Pakchong, Purple, Kobe, Indian, and Dwarf Napier grass were grown in a completely randomized design with three replications to determine their soluble oxalate, total oxalate, and silica contents. Plants were harvested at two months of plant maturity. Whole plant of the Dwarf Napier grass contained significantly higher soluble oxalate content than tall varieties. Total oxalate content in whole plant differed significantly among varieties. Dwarf showed the highest total oxalate content (3.23% dry matter (DM)) followed by Kobe (2.61%), Zanzibar (2.60%), Purple (2.44%), Taiwan (2.43%), Indian (2.15%), and Pakchong (1.95%). Regardless of variety, leaf tissue contained significantly higher soluble oxalate and total oxalate than stem tissue. There were no differences in silica content among them. In conclusion, the tall varieties could produce lower levels of soluble oxalate than the Dwarf variety, whereas silica content might not vary among them.

    DOI: 10.4314/sajas.v50i3.6

    Scopus

  • Effects of diets containing asyastasia gangetica and brachiaria decumbens on intake, digestibility and growth performance of growing rabbits

    Rahman M.M., Ismail S.N.B., Mat K., Gondo T., Nor M.M., Akashi R.

    Pertanika Journal of Tropical Agricultural Science   43 ( 4 )   609 - 619   2020年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Pertanika Journal of Tropical Agricultural Science  

    The beneficial effects of feeding rabbits with forages have been recommended. However, limited study has been made on feeding values of locally available forages in rabbits. Therefore, an experiment was conducted to evaluate the effect of Asyastasia gangetica and Brachiaria decunbens on intake, digestibility, and growth performance of rabbits. Twelve rabbits were distributed into three diets: (i) 100% commercial pellet as control (T1), (ii) 50% pellet plus Asyastasia gangetica ad libitum (T2), and (iii) 50% pellet plus Brachiaria decumbens ad libitum (T3). Daily feed intake, nutrient digestibility, weekly body weight, and feed conversion ratio (FCR) were measured. Intakes of total dry matter (DM) (121.2-134.3 g/d) and organic matter (OM) (115.1-132.5 g/d) were similar (p>0.05) for all the groups. The crude protein (CP) intake of rabbits fed with T1 (10.1 g/d) and T3 (9.6 g/d) diets was similar (p>0.05), but lower (p<0.05) than T2 (14.1 g/d) diet. Ether extract intake of rabbits fed with T2 (2.9 g/d) and T3 (3.9 g/d) diets was similar (p>0.05), but lower (p<0.05) than T1 (5.1 g/d) diet. Neutral detergent fibre (NDF) intake was higher (p<0.05) for rabbits fed with T3 (55.8 g/d) diet followed by T2 (41.7 g/d) and T1 (31.7 g/d) diets. There were no differences (p>0.05) on the digestibilities of DM, OM, and CP among treatments. Total weight gain and daily weight gain were higher (p<0.05) for rabbits fed with T1 (568 and 11.0 g) and T2 (468 and 9.0 g) diets than T3 (155 and 3.3 g) diet, respectively. The lowest FCR was obtained with T1 (12.3) and T2 (13.9) diets, whereas the highest was obtained with T3 (30.3) diet. In conclusion, diet containing Asyastasia gangetica showed more benefits in terms of CP and NDF intakes, weight gain and FCR than the diet containing Brachiaria decumbens. A combination of concentrate and Asyastasia gangetica is recommended as a partial replacement for concentrate in rabbit production.

    DOI: 10.47836/PJTAS.43.4.14

    Scopus

  • Asparaginyl-tRNA synthetase gene (SYNC1) characterized by lotus corniculatus FOX-superroot lines has effects on plant morphology and amino acid contents of seed in soybean 査読あり

    Arifin A., Gondo T., Akashi R.

    Plant Biotechnology   36 ( 4 )   233 - 240   2019年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Plant Biotechnology  

    © 2019 The Japanese Society for Plant Cell and Molecular Biology. The application of useful genes from model plants to crops is an important step to verify its agricultural usefulness. SYNC1, an asparaginyl-tRNA synthetase gene, was previously identified through the Full-length cDNA Over-eXpressor gene (FOX gene-hunting system) of Arabidopsis cDNA by using super-growing root (SR) culture of Lotus corniculatus, and was suggested to have a potential in increasing some amino acid contents and plant biomass. To identify the functionality of SYNC1 gene in a typical legume crop soybean, the effects of its overexpression in transgenic plants to agricultural traits and free amino acid contents were evaluated. The transgenic soybean plants were produced from infected half-seed explants of 1 day old seedlings with the suspension of Agrobacterium tumefaciens harboring expression vector pB7WG2D-SYNC1. The transgenic plants that overexpressed SYNC1 gene had increased asparagine and lysine contents in matured seeds, and increased aspartate, lysine, alanine and histidine contents in germinated seeds. The changes in those free amino acid contents affected plant morphology and led to significant increase in plant length, number of branches and number of branch nodes as yield components of soybean. The transgenic plants also showed a tendency of higher number of pods, seeds and total seed weight per plant. These results showed that the overexpression of SYNC1 gene contributes on the increase of plant free amino acid contents and biomass, and this approach is expected to be applicable in other legumes, grain and forage crops.

    DOI: 10.5511/plantbiotechnology.19.1016a

    Scopus

    PubMed

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書籍等出版物 【 表示 / 非表示

  • 形質転換プロトコール(植物編)「2章2-3バヒアグラス」

    権藤 崇裕、明石 良( 担当: 分担執筆)

    化学同人  2012年9月 

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    総ページ数:412   担当ページ:97-105   記述言語:日本語 著書種別:学術書

MISC 【 表示 / 非表示

  • 暖地型イネ科牧草類のバイオ技術 ―組織培養・遺伝子組換え技術の確立からゲノム編集技術の開発まで― 招待あり

    権藤 崇裕

    アグリバイオ   3 ( 10 )   975 - 980   2019年9月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

  • バイオリソース&データベース活用術〜ミヤコグサ・ダイズ モデル植物から作物への展開 招待あり

    橋口正嗣、権藤崇裕、明石 良

    細胞工学 別冊   212 - 215   2009年7月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

  • バイオテクノロジーを利用した暖地型イネ科牧草の育種(<特集>イネ科牧草の遺伝育種の展開)

    明石 良, 権藤 崇裕, 川村 修

    日本草地学会誌   49 ( 1 )   79 - 87   2003年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)   出版者・発行元:日本草地学会  

    DOI: 10.14941/grass.49.79

講演・口頭発表等 【 表示 / 非表示

  • 2017年中に発表されたゲノム編集イネのオフターゲット効果に関する評価

    成島純平、中村公亮 他

    日本農芸化学会大会 

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    開催年月日: 2020年3月25日 - 2020年3月28日

    記述言語:日本語   会議種別:口頭発表(一般)  

  • バヒアグラスにおけるリグニン生合成に関わるCAD遺伝子のゲノム編集

    権藤崇裕、湯浅玲奈、明石良

    第76回日本草地学会 

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    開催年月日: 2020年

    記述言語:日本語   会議種別:口頭発表(一般)  

  • Asparaginyl-tRNA synthetase gene (SYNC1) characterized by lotus corniculatus FOX-superroot lines has effects on plant morphology and amino acid contents of seed in soybean

    Azeri Gautama Arifin, Takahiro Gondo, Ryo Akashi

    第37回日本植物細胞分子生物学会 

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    開催年月日: 2019年9月7日 - 2019年9月8日

    記述言語:英語   会議種別:口頭発表(一般)  

  • バヒアグラス遺伝子組換え体におけるCAD遺伝子の発現抑制がPAL、COMT遺伝子の発現とリグニン含量に及ぼす影響

    権藤崇裕、山田智仁、湯浅玲奈、明石良

    日本草地学会大会 

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    開催年月日: 2019年

    記述言語:日本語   会議種別:口頭発表(一般)  

  • コウシュンシバにおけるアグロバクテリウム法による遺伝子組換え系の確立

    牛山真里、張震、明石良、権藤崇裕

    第36回日本植物細胞分子生物学会 

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    開催年月日: 2019年

    記述言語:日本語   会議種別:ポスター発表  

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科研費(文科省・学振・厚労省)獲得実績 【 表示 / 非表示

  • 植物の遺伝子組換え技術について学ぼう!

    2019年

    科学研究費補助金  研究成果公開促進費・研究成果公開発表(B)

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    担当区分:研究代表者 

  • 植物の遺伝子組換え技術について学ぼう!

    2018年

    科学研究費補助金  研究成果公開促進費・研究成果公開発表(B)

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    担当区分:研究代表者 

  • 牧草・芝草におけるゲノム編集による新たな育種技術の基盤構築

    2017年 - 2020年03月

    科学研究費補助金  基盤研究(C)

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    担当区分:研究代表者 

  • 植物の遺伝子組換え技術について学ぼう!

    2017年

    科学研究費補助金  研究成果公開促進費・研究成果公開発表(B)

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    担当区分:研究代表者 

  • 植物の遺伝子組換え技術について学ぼう!

    2016年

    科学研究費補助金  研究成果公開促進費・研究成果公開発表(B)

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    担当区分:研究代表者 

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寄附金・講座・研究部門 【 表示 / 非表示

  • 伊藤記念財団研究助成

     2021年06月

  • 伊藤記念財団研究助成

     2020年06月