論文 - 秋枝 さやか
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Akieda-Asai S., Zaima N., Ikegami K., Kahyo T., Yao I., Hatanaka T., Iemura S., Sugiyama R., Yokozeki T., Eishi Y., Koike M., Ikeda K., Chiba T., Yamaza H., Shimokawa I., Song S., Matsuno A., Mizutani A., Sawabe M., Chao M., Tanaka M., Kanaho Y., Natsume T., Sugimura H., Date Y., Mcburney M., Guarente L., Setou M.
PLoS ONE 5 ( 7 ) 2010年8月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：PLoS ONE
Background: SIRT1, a NAD-dependent deacetylase, has diverse roles in a variety of organs such as regulation of endocrine function and metabolism. However, it remains to be addressed how it regulates hormone release there. Methodology/Principal Findings: Here, we report that SIRT1 is abundantly expressed in pituitary thyrotropes and regulates thyroid hormone secretion. Manipulation of SIRT1 level revealed that SIRT1 positively regulated the exocytosis of TSH-containing granules. Using LC/MS-based interactomics, phosphatidylinositol-4-phosphate 5-kinase (PIP5K)y was identified as a SIRT1 binding partner and deacetylation substrate. SIRT1 deacetylated two specific lysine residues (K265/K268) in PIP5Ky and enhanced PIP5Ky enzyme activity. SIRT1-mediated TSH secretion was abolished by PIP5Ky knockdown. SIRT1 knockdown decreased the levels of deacetylated PIP5Ky, PI(4,5)P 2 , and reduced the secretion of TSH from pituitary cells. These results were also observed in SIRT1-knockout mice. Conclusions/Significance: Our findings indicated that the control of TSH release by the SIRT1-PIP5Kγ pathway is important for regulating the metabolism of the whole body. © 2010 Akieda-Asai et al.
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秋枝 さやか, 伊達 紫
化学と生物 47 ( 11 ) 750 - 755 2009年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Japan Society for Bioscience, Biotechnology, and Agrochemistry
生体は，日々のエネルギー摂取の変動にもかかわらず，一定の体重を維持するためのエネルギー恒常性機構を有している．エネルギーを獲得する唯一の方法である摂食行動は，空腹や満腹を伝える液性因子の情報が中枢に到達し，記憶，学習，認知，情動などの高次機能と統合され，神経ネットワークが作動することで巧妙に制御されている．さらに神経ネットワークからの出力は，末梢の代謝関連臓器である脂肪組織，骨格筋，肝臓，消化管などで行なわれる細胞レベルでのエネルギー代謝調節に寄与している．このように，生体が効率よくエネルギー恒常性を維持するためには，各臓器によるクロストーク機構が重要であるとの認識から，近年，多臓器間ネットワークによる代謝連関のメカニズムに関する研究が活発に行なわれている．ここでは，空腹や満腹シグナルとして機能するホルモンの中枢への伝達経路のひとつである迷走神経求心路に焦点をあてて，主に食行動における末梢と中枢の機能連関について解説する．
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Layer-specific sulfatide localization in rat hippocampus middle molecular layer is revealed by nanoparticle-assisted laser desorption/ionization imaging mass spectrometry. 査読あり
Ageta H*, Asai S*, Sugiura Y, Goto-Inoue N, Zaima N, Setou M
Medical Molecular Morphology 42 ( 1 ) 16 - 23 2009年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Effects of transmaternal exposure to genistein in Hatano high- and low-avoidance rats 査読あり
Ohta R, Shirota M, Kanazawa Y, Shindo T, Furuya M, Seki T, Ono H, Kojima K, Asai S, Watanabe G, Taya K
Experimental Animals 58 ( 5 ) 471 - 479 2009年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Single-cell imaging of c-fos expression in rat primary hippocampal cells using a luminescence microscope 査読あり
Asai S, Takamura K, Suzuki H, Setou M
Neuroscience Letters 434 ( 3 ) 289 - 292 2008年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Gastric ulceration and expression of prolactin receptor in the brain in Hatano high- and low- avoidance rats. 査読あり
Asai S, Ohta R, Fujikawa T, Sakai RR, Shirota M, Ogata M, Watanabe G, Taya K.
Endocrine 30 ( 2 ) 161 - 166 2006年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Prolactin as an anti-stress hormone by increasing effect of ACTH in adrenalgland of rats: the Hatano rats as an excellent animal model 査読あり
Jaroenporn S, Nagaoka K, Kasahara C, Asai S, Ohta R, Watanabe G, Taya K
Comparative Endocrinology and Biodiversity in Asia and Oceania 281 - 286 2006年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Endocrinological responses during suckling in Hatano high- and low- avoidance rats. 査読あり
Asai S, Ohta R, Shirota M, Tohei A, Watanabe G, Taya K.
Journal of Endocrinology 182 ( 2 ) 267 - 272 2004年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Differential responses of the hypothalamo-pituitary-adrenocortical axis to acute restraint stress in Hatano high- and low-avoidance rats. 査読あり
Asai S, Ohta R, Shirota M, Watanabe G, Taya K.
Journal of Endocrinology 181 ( 3 ) 515 - 520 2004年6月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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NMDA receptor antagonists reduce restraint-induced release of prolactin in male rats 査読あり
Liu JX, Du JZ, Asai S, Shi ZQ, Watanabe G, Taya K
Neuroendocrinology Letters 24 ( 6 ) 435 - 439 2003年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Effects of reduction of the number of primordial follicles on follicular development to achieve puberty in female rats 査読あり
Shirota M, Soda S, Katoh C, Asai S, Sato M, Ohta R, Watanabe G, Taya K, Shirota K
Reproduction 125 ( 1 ) 85 - 94 2003年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Yoshida M., Katsuda S., Tanimoto T., Asai S., Nakae D., Kurokawa Y., Taya K., Maekawa A.
Carcinogenesis 23 ( 10 ) 1745 - 1750 2002年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Carcinogenesis
Inappropriate exposure to estrogens in the fetal and/or newborn period can exert irreversible influence, including carcinogenesis on the reproductive system in mammals. The present study was conducted to investigate uterine carcinogenesis in Donryu rats treated neonatally with a high-dose estrogenic compound, p-t-octylphenol (OP) for different exposure periods. Female Donryu. rats were subcutaneously administered 100 mg/kg/day OP every other day for the first 5 postnatal days (PNDs 1-5) or the first 2 weeks (PNDs 1-15). They received a single injection of 20 mg/kg N-ethyl-N′-nitro-N-nitrosoguanidine (ENNG) into a uterine horn at 11 weeks of age and were examined until 15 months of age. PNDs 1-5 OP-treated rats showed normal development of the female reproductive system, including uterine gland genesis and normal estrous cycling after vaginal opening. The treatment, however, accelerated an earlier occurrence of persistent estrus and increased the number of well differentiated uterine adenocarcinomas as compared with controls. This indicated that PNDs 1-5 OP treatment acts as a delayed modulator of the hypothalamus-pituitary-ovarian hormonal control system and the modulation increased the serum estrogen:progesterone ratio, resulting in induction of uterine tumors. On the contrary, PNDs 1-15 OP treatment demonstrated immediate and irreversible influences on the control system, called 'androgenization', and induced abnormal uterine development manifested by prolonged persistent estrus immediately after vaginal opening and also suppression of uterine gland genesis. In addition, uterine tumor malignancy in morphological and biological property clearly increased in this group although the total number of adenocarcinomas was not increased. The present study provides evidence that neonatal exposure to a high-dose OP enhances uterine carcinogenesis in rats, and the type of uterine tumors is changed by the periods of neonatal exposure to OP, suggesting that the mechanism of uterine tumor development is dependent upon neonatal exposure periods.
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Induction of different types of uterine adenocarcinomas in Donryu rats due to neonatal exposure to high-dose p-t-octylphenol for different periods 査読あり
Yoshida M, Katsuda S, Tanimoto T, Asai S, Nakae D, Kurokawa Y, Taya K, Maekawa A
Carcinogenesis 23 ( 10 ) 1745 - 1750 2002年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Reproductive endocrinology in Hatano high- and low- avoidance rats during the estrous cycle 査読あり
Asai S, Ohta R, Shirota M, Sato M, Watanabe G, Taya K
Endocrine 18 ( 2 ) 161 - 166 2002年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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A comparative study of puberty, and plasma gonadotropin and testicular hormone levels in two inbred strains of Hatano rats. 査読あり
Sato M, Ohta R, Kojima K, Shirota M, Koibuchi H, Asai S, Watanabe G, Taya K
Journal of Reproduction and Development 48 ( 2 ) 111 - 119 2002年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Sato M., Ohta R., Kojima K., Shirota M., Koibuchi H., Asai S., Watanabe G., Taya K.
Journal of Reproduction and Development 48 ( 2 ) 111 - 119 2002年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Reproduction and Development
We found that two inbred strains of Hatano rats bred selectively for shuttlebox avoidance responses, had different characteristics of responses to stress and adrenal function. The characteristics of male puberty, including preputial separation, sperm number, and plasma hormone levels during development, were investigated and compared between the two strains. Body weight in high-avoidance (HAA) rats was consistently heavier than that in low-avoidance (LAA) rats. Although the absolute testicular weight was heavier in HAA rats than in LAA rats during the juvenile period, after the peripubertal period the inverse finding was observed. Preputial separation occurred about 7 days earlier in HAA rats than in LAA rats. The testicular and epididymal sperm head number in HAA rats was greater than that in LAA rats during the peripubertal period, but there were no differences after sexual maturation. A steep increment of plasma gonadotropin levels was observed in HAA rats, and the levels were higher in HAA rats than in LAA rats during the peripubertal period. Plasma testosterone levels began to rise at a younger age in HAA rats than in LAA rats. It was concluded that HAA males sexually mature earlier than LAA males, and that these apparent strain differences in puberty are primarily dependent on gonadotropin levels during sexual maturation.
DOI： 10.1262/jrd.48.111
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Effects of Hong-jing-tian (Rhodiola sachalinensis A.Bor) on mammary gland and related parameters in mice. 査読あり
Hiroshi Nagasawa, Sayaka Asai, Hideo Inatomi.
和漢医薬学雑誌 16 ( 1 ) 38 - 43 1999年4月
記述言語：日本語 掲載種別：研究論文（学術雑誌）