|
Affiliation |
Faculty of Medicine School of Medicine Department of Medical Sciences, Integrative Physiology |
|
Title |
Associate Professor |
|
External Link |
|
|
Related SDGs |
|
WAKAZONO Yoshihiko
|
|
|
Degree 【 display / non-display 】
-
博士（学術） ( 1997.9 総合研究大学院大学 )
-
修士（薬学） ( 1994.3 静岡県立大学 )
-
学士（薬学） ( 1992.3 静岡県立大学 )
Research Areas 【 display / non-display 】
-
Life Science / Neuroscience-general
Papers 【 display / non-display 】
-
Wakazono Y, Midorikawa R, Takamiya K
Neuroscience Research 198 21 - 29 2024.1
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Neuroscience Research
In the present study, we attempted to temporally and quantitatively analyze the functional contributions of Ca2+-permeable (CP) α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) during long-term potentiation (LTP) expression using electrophysiological and pharmacological approaches. In hippocampal CA1 neurons, using 1-naphthyl acetyl spermine (NASPM), a CP-AMPAR antagonist, we began by demonstrating that NASPM-sensitive components, probably including the GluA1 homomer, functionally contributed to about 15% of AMPAR-mediated EPSC amplitude in basal conditions. Then, when NASPM was treated at different time points (3–30 min) after LTP induction, it was found that LTP was almost completely impaired at 3 or 10 min but maintained at 20 or 30 min, although its potentiation was reduced. Further temporal and quantitative analysis revealed that the functional expression of CP-AMPARs began increasing approximately 20 min after LTP induction and reached more than twice the basal level at 30 min. These results suggest that CP-AMPARs in the first 3–10 min of LTP might play an important role in LTP maintenance. Moreover, their decay time was also significantly increased at 30 min, suggesting that CP-AMPARs changed not only quantitatively in LTP but also qualitatively.
-
Suppression of ciliary movements by a hypertonic stress in the newt olfactory receptor neuron Reviewed
Yoshihiko Wakazono, Takashi Sakurai, Susumu Terakawa
Am J Physiol Cell Physiol 2017.10
Authorship：Lead author Publishing type：Research paper (scientific journal)
-
Midorikawa R., Wakazono Y., Takamiya K.
Journal of Cell Science 137 ( 8 ) 2024
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Cell Science
Amyloid β (Aβ) is a central contributor to neuronal damage and cognitive impairment in Alzheimer’s disease (AD). Aβ disrupts AMPA receptor-mediated synaptic plasticity, a key factor in early AD progression. Numerous studies propose that Aβ oligomers hinder synaptic plasticity, particularly long-term potentiation (LTP), by disrupting GluA1 (encoded by GRIA1) function, although the precise mechanism remains unclear. In this study, we demonstrate that Aβ mediates the accumulation of GM1 ganglioside in lipid raft domains of cultured cells, and GluA1 exhibits preferential localization in lipid rafts via direct binding to GM1. Aβ enhances the raft localization of GluA1 by increasing GM1 in these areas. Additionally, chemical LTP stimulation induces lipid raft-dependent GluA1 internalization in Aβ-treated neurons, resulting in reduced cell surface and postsynaptic expression of GluA1. Consistent with this, disrupting lipid rafts and GluA1 localization in rafts rescues Aβ-mediated suppression of hippocampal LTP. These findings unveil a novel functional deficit in GluA1 trafficking induced by Aβ, providing new insights into the mechanism underlying AD-associated cognitive dysfunction.
DOI： 10.1242/jcs.261281
-
Monitoring the glycosylation of AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazole-propionate )-type glutamate receptors using specific antibodies reveals a novel regulatory mechanism of N-glycosylation occupancy by molecular chaperones in Mice. Reviewed
Midorikawa R, Takakura D, Morise J, Wakazono Y, Kawasaki N, Oka S, Takamiya K
Journal of neurochemistry e14964 2020.1
-
AMPA receptors in the synapse turnover by monomer diffusion. Reviewed
Morise J, Suzuki KGN, Kitagawa A, Wakazono Y, Takamiya K, Tsunoyama TA, Nemoto YL, Takematsu H, Kusumi A, Oka S
Nat Commun. 10 ( 1 ) 2019.11
Language：English Publishing type：Research paper (scientific journal)
Books 【 display / non-display 】
-
無敵のバイオテクニカルシリーズ（特別編）脳・神経研究の進めかた「基礎技術・細胞培養法」 （共著）
南木浩二、若園佳彦、池中一裕（ Role： Joint author）
羊土社 1998.5
Language：Japanese
MISC 【 display / non-display 】
-
エバネッセンス顕微鏡 （共著）
寺川 進、桜井孝司、坪井貴司、菊田敏輝、若園佳彦、山本清二
生体の科学 2003.5
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：医学書院
-
小脳顆粒細胞分化・成熟と電位依存性カリウムチャネルの発現 （共著）
中平健祐、柴田理一、若園佳彦、池中一裕
蛋白質・核酸・酵素 2000.5
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：共立出版
-
小脳顆粒細胞の成熟 （共著）
若園佳彦、池中一裕
生理学研究所年報 1996.5
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (other) Publisher：岡崎国立共同研究機構 生理学研究所
-
神経発生とカリウムチャネル （共著）
池中一裕、若園佳彦、秋田裕美、中平健祐
神経研究の進歩 1995.5
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：医学書院
Presentations 【 display / non-display 】
-
AMPA受容体のN型糖鎖修飾のシナプス機能における生理学的役割
若園佳彦、緑川良介、髙宮考悟
APPW 2025（第１３０回 日本解剖学会／第１０２回 日本生理学会／第９８回 日本薬理学会合同学会）
Event date： 2025.3.17 - 2025.3.19
Language：English Presentation type：Poster presentation
-
Physiological roles of the N-glycosylation of the AMPA receptor in synaptic function
若園佳彦、緑川良介、髙宮考悟
NEURO 2024（第４７回 日本神経科学大会／第６７回 日本神経化学会大会）
Event date： 2024.7.24 - 2024.7.27
Language：English Presentation type：Poster presentation
-
Functional roles and temporal contribution of Ca2+-permeable AMPA receptors in LTP expression
若園佳彦、緑川良介、髙宮考悟
第４６回 日本神経科学大会
Event date： 2023.8.1 - 2023.8.4
Language：English Presentation type：Poster presentation
-
長期増強におけるカルシウム透過性AMPA受容体の機能発現の経時的および定量的解析
若園佳彦、緑川良介、髙宮考悟
第１００回 日本生理学会大会
Event date： 2023.3.14 - 2023.3.16
Language：English Presentation type：Poster presentation
-
A crucial role of N-glycosylation of homomeric GluA1 AMPA receptor in LTP induction
Yoshihiko Wakazono, Ryosuke Midorikawa, Shogo Oka, Kogo Takamiya
第97回日本生理学会大会 (大分県別府市) 日本生理学会
Event date： 2020.3.17 - 2020.3.19
Language：English Presentation type：Poster presentation
Venue：大分県別府市
Grant-in-Aid for Scientific Research 【 display / non-display 】
-
グルタミン酸受容体の糖鎖修飾による脳高次機能と精神疾患への関与
Grant number：21K07483 2021.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
高宮 考悟、
Authorship：Coinvestigator(s)
-
自由行動下、細胞レベルでのｉｎ ｖｉｖｏイメージング装置の開発と場所細胞への適用
Grant number：24650209 2012.04 - 2014.03
科学研究費補助金 挑戦的萌芽研究
Authorship：Principal investigator
自由行動下、細胞レベルでのｉｎ ｖｉｖｏイメージング装置の開発と場所細胞への適用