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Faculty of Agriculture Region of Veterinary Science |
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Professor |
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Related SDGs |
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UEMURA Ryoko
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Research Areas 【 display / non-display 】
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Life Science / Veterinary medical science
Papers 【 display / non-display 】
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You G., Uemura R., Shimizu K., Hoshinoo K., Ueno Y., Takamatsu D., Iguchi A.
Infection Genetics and Evolution 143 105985 2026.9
Language：English Publishing type：Research paper (scientific journal) Publisher：Infection Genetics and Evolution
The genus Mannheimia comprises Gram-negative bacteria that primarily inhabit the upper respiratory tract of ruminants. Currently, 10 species are recognized within the genus Mannheimia, among which M. haemolytica has been extensively studied due to its association with bovine respiratory disease (BRD). In contrast, information on other species, particularly the four species proposed since 2021 (M. pernigra, M. bovis, M. cairinae, and M. indoligenes), remains extremely limited. In this study, we retrospectively analyzed 210 Mannheimia isolates obtained from nasal swabs of cattle in Japan between 2010 and 2020. Based on 16S rRNA gene sequence comparisons, 173 isolates showed ≥98.65% sequence similarity to their respective type strains and were classified into six species: M. haemolytica (n = 98), M. varigena (n = 30), M. pernigra (n = 21), M. indoligenes (n = 21), M. granulomatis (n = 2), and M. ruminalis (n = 1). The remaining 37 isolates showed <98.65% sequence similarity and were classified as related to four species: M. bovis (n = 19), M. indoligenes (n = 15), M. varigena (n = 2), and M. ruminalis (n = 1). A newly developed PCR assay revealed that all isolates, except for one M. ruminalis isolate, one M. ruminalis-related isolate, and four M. bovis-related isolates, carried the lktA gene encoding leukotoxin, a major virulence factor. These findings indicate that multiple Mannheimia species are widely distributed among cattle in Japan. Notably, some isolates showing <98.65% 16S rRNA gene sequence similarity to known type strains may represent novel taxa, warranting taxonomic re-evaluation and phylogenetic reassessment within Mannheimia. Moreover, because many of these isolates harbor key virulence-associated genes linked to BRD, further studies are required to clarify the pathogenic potential of each species as opportunistic pathogens and their contribution to BRD.
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Kanda T., Uemura R., Ito K., Tanaka K., Sakudo A.
Journal of Microbiological Methods 247 107562 2026.8
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Microbiological Methods
Some metabolic enzymes in Mycoplasma bovis function associated with pathogenicity, making metabolic activity indispensable for both survival and infection strategies. Quantitative evaluation of both growth and metabolic activity is essential for characterizing Mycoplasma bovis. We developed an automated image analysis system utilizing a standard flatbed scanner as a substitute for spectrophotometry. By leveraging this accessible OA equipment, we established a platform to quantify the correlation among inoculum size, growth activity, and metabolic power (MP) through pH and redox kinetics. For six strains (PG45 and wild-type S1–S5), we compared and analyzed pH-MP and redox-MP, defined by linear regression between the initial inoculum and detection threshold time, with the initial growth rate. In growth assays, asymptomatic isolates showed an initial lag phase (0−20h), whereas clinical isolates exhibited a late deceleration phase (40–60 h); however, no significant differences in the maximum bacterial titer were observed between strains. The scanner-based system demonstrated high quantitative accuracy, achieving R<sup>2</sup> values of 0.97–0.98 (PG45). Significant strain-specific differences were observed in growth and metabolism; the asymptomatic isolate S2 exhibited the lowest growth rate (0.04) as well as high pH-MP (0.21) and redox-MP (0.17). Furthermore, strain S3 exhibited metabolic bias, with pH-MP (0.17) exceeding redox-MP (0.12). By monitoring pH and redox kinetics, this system could visualize strain-specific metabolic activity. Although the current findings are based on a limited number of strains, this study establishes a robust tool for metabolic biotyping via phenotypic kinetic profiles. This platform enables high-throughput screening to prioritize strains for subsequent detailed molecular characterization, such as transcriptomics; integrating these kinetics with molecular data will facilitate the elucidation of mechanisms linking microbial physiology to actual pathogenicity.
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A highly sensitive method for detecting African swine fever virus in oral fluids from naturally infected pigs in Northern Vietnam Reviewed International coauthorship
Thi Ngan Mai, Thi Huong Giang Tran, Van Hieu Dong, Van Phan Le, Thi My Le Huynh, Tran Anh Dao Bui, Ryoko Uemura, Yasuko Yamazaki, Thi Lan Nguyen & Wataru Yamazaki
Scientific Reports 15 27855 2025.7
Language：English Publishing type：Research paper (scientific journal)
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Comprehensive serotyping of Mannheimia haemolytica by a PCR system using the diversity of capsule biosynthesis genes. Reviewed
Iguchi A, Ueno Y, Hoshinoo K, Okuno M, Uemura R, You G, Ogura Y, Takamatsu D
Scientific reports 15 ( 1 ) 11970 2025.4
Language：English Publishing type：Research paper (scientific journal)
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Biofilm characterisation of Mycoplasma bovis co-cultured with Trueperella pyogenes Reviewed
Nishi K., Gondaira S., Hirano Y., Ohashi M., Sato A., Matsuda K., Iwasaki T., Kanda T., Uemura R., Higuchi H.
Veterinary research 56 ( 1 ) 22 2025.1
Language：English Publishing type：Research paper (scientific journal) Publisher：Veterinary research
Mycoplasma pneumonia, caused by Mycoplasma bovis (Mycoplasmopsis bovis; M. bovis), is linked with severe inflammatory reactions in the lungs and can be challenging to treat with antibiotics. Biofilms play a significant role in bacterial persistence and contribute to the development of chronic lesions. A recent study has shown that polymicrobial interactions between species are an important factor in biofilm formation, yet the precise mechanism of biofilm formation in M. bovis remains unknown. By assuming multiple pathogen infections in the bovine respiratory disease complex (BRDC), this study examined the characterisation of the polymicrobial relationship between M. bovis and Trueperella pyogenes (T. pyogenes) during biofilm formation. Autopsies were performed on four Holstein calves (two chronic Mycoplasma pneumonia calves and two control calves). Bacterium-like aggregation structures (> 10 μm), which were assumed to be biofilms of M. bovis in vivo, were observed adhering to the cilia in calves with Mycoplasma pneumonia. M. bovis released an extracellular matrix to connect with neighbouring bacteria and form a mature biofilm on the plate. Biofilm formation in the co-culture of M. bovis and T. pyogenes (strain T1: 1 × 105 and 1 × 106 CFU/well) significantly increased (p < 0.05 and p < 0.01; 64.1% and 64.8% increase) compared to that in a single culture of these bacteria. Furthermore, some large aggregates (> 40 μm), composed of M. bovis and T. pyogenes, were observed. The morphological characteristics of this biofilm were similar to those observed in vivo compared to a single culture. In conclusion, the polymicrobial interaction between M. bovis and T. pyogenes induces biofilm formation, which is associated with increased resistance to antimicrobial agents, and this exacerbates the progression of chronic Mycoplasma pneumonia.
Books 【 display / non-display 】
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動物衛生学 第2版
伊東拓也 河合一洋 末吉益雄 高井信二 樋口豪紀 山口剛士 編集（ Role： Contributor , 第10-2. 肉牛の管理衛生 -1)飼養状況と飼養形態）
文永堂出版 2024.3
Language：Japanese Book type：Textbook, survey, introduction
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動物衛生学 第2版
伊東拓也 河合一洋 末吉益雄 高井信二 樋口豪紀 山口剛士 編集（ Role： Contributor , コラム 養豚場における抗菌剤使用低減への取り組み）
文永堂出版 2024.3
Language：Japanese Book type：Textbook, survey, introduction
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動物衛生学 第2版
伊東拓也 河合一洋 末吉益雄 高井信二 樋口豪紀 山口剛士 編集（ Role： Contributor , 第10-2. 肉牛の管理衛生 -2)疾病の発生状況）
文永堂出版 2024.3
Language：Japanese Book type：Textbook, survey, introduction
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獣医微生物学第5版
関崎勉、遠矢幸伸、福士秀人、堀本泰介、村瀬敏之 編集（ Role： Contributor）
文永堂出版 2024
Total pages：514 Responsible for pages：204-210 Language：Japanese Book type：Textbook, survey, introduction
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動物の衛生 第2版
（ Role： Contributor , 第4章2 栄養と代謝障害）
文永堂出版 2020.3
Language：Japanese Book type：Textbook, survey, introduction
MISC 【 display / non-display 】
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牛の常在細菌叢 呼吸器の細菌叢 Invited Reviewed
上村涼子
臨床獣医 42 ( 4 ) 30 - 34 2024.4
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
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正確な検査結果のための現場におけるサンプリング 鼻腔拭い液のサンプリング Invited
上村涼子
臨床獣医 40 ( 4 ) 26 - 29 2022.4
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：緑書房
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鼻汁などから容易に感染、マイコプラズマ性肺炎に注意 Invited
上村涼子
DAIRYMAN 68 ( 8 ) 42 - 43 2018.8
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：デーリィマン社
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ロープワークをマスターしよう⑦ 尾の保定その1 尾房の結び方 Invited
上村涼子
DAIRYMAN 68 ( 8 ) 59 - 59 2018.8
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：デーリィマン社
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ロープワークをマスターしよう⑥ 柵への係留その2 もやい結びと馬結び Invited
上村涼子
DAIRYMAN 68 ( 7 ) 65 - 65 2018.7
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：デーリィマン社
Presentations 【 display / non-display 】
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Mycoplasma bovisのフルオロキノロン系抗菌薬耐性に関する研究
小野有菜、上村涼子
第14回家畜感染症学会学術集会 2024.12.14
Event date： 2024.12.14 - 2024.12.15
Language：Japanese Presentation type：Oral presentation (general)
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豚の浮腫病由来志賀毒素産生性大腸菌のファージ感染力価測定法の確立
有賀士朗、上村涼子
令和6年度獣医学術九州地区学会 2024.11.1
Event date： 2024.11.1
Language：Japanese Presentation type：Oral presentation (general)
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Development of a simple and highly sensitive method for the concentration and detection of African swine fever virus in oral fluids and its feasibility assessment on raised pigs in Northern Vietnam International coauthorship International conference
Wataru YAMAZAKI, Ngan MAI THI, Giang TRAN THI HUONG, Hieu DONG VAN, Phan LE VAN, Le HUYNH THI MY, Dao BUI TRAN ANH , Ryoko UEMURA, Yasuko YAMAZAKI, Lan NGUYEN THI
8th World One Health Congress 2024.9
Event date： 2024.9.20 - 2024.9.23
Language：English Presentation type：Oral presentation (general)
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グルコースは豚の浮腫病由来STECの毒素産生を促進する
有賀士朗、上村涼子
九州微生物研究フォーラム 2024 2024.9.13
Event date： 2024.9.13 - 2024.9.14
Language：Japanese Presentation type：Oral presentation (general)
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Assessment the risk of Mycoplasma bovis resistance to fluoroquinolones based on MIC and MPC values
Ono Arina, Uemura Ryoko
2024.5.17
Event date： 2024.5.17 - 2024.5.18
Language：Japanese Presentation type：Oral presentation (general)
Awards 【 display / non-display 】
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令和5年度日本産業動物獣医学九州地区学会 学会長賞
2023.9 令和5年度日本産業動物獣医学九州地区学会 Mycoplasma bovis によるデキサメサゾン感作牛好中球の機能抑制
石崎絢太、末吉益雄、上村涼子
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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令和4年度日本産業動物獣医学九州地区学会連合会長賞
2022.10 令和4年度日本産業動物獣医学九州地区学会 培養基中の植物性蛋白質は豚の浮腫病由来志賀毒素産生性大腸菌の志賀毒素産生を増加させる
永田舜、末吉益雄、上村涼子
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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第163回獣医学会微生物分科会若手奨励賞
2020.9 日本獣医学会微生物分科会 牛好中球へのMycoplasma bovis感作の影響
上村悠祐, 上村涼子, 小林郁雄, 末吉益雄.
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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第162回獣医学会微生物分科会若手奨励賞
2019.9 日本獣医学会微生物分科会 Mycoplasma bovis感作に対する牛肺胞マクロファージのサイトカイン発現応答
甲斐慎二, 上村涼子, 石川真悟, 帆保誠二, 小林郁雄, 保田昌宏, 末吉益雄.
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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平成29年日本家畜臨床学会長賞
2017.11 日本家畜臨床学会
國保健浩、上村涼子 ほか
Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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牛マイコプラズマ肺炎の病態診断に関する研究
Grant number：15K18785 2015.04 - 2018.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
牛マイコプラズマ肺炎は、主にMycoplasma bovisを原因菌とする感染症で、発症すると不可逆的な乾酪壊死性肺炎の経過をたどって廃用淘汰されるなど経済的損失が大きく、近年特に問題となっている。本病の最も大きな問題点は、①健常牛からもM. bovisが分離され発症が日和見的であること、②病態把握が難しく結果的に長期加療の上で廃用となるため、治療費、飼料代、労力などの経済的損失が大きいこと、③さらにその発症牛が農場内の感染源となり続けることがあげられる。この課題解決のために、１）牛マイコプラズマ肺炎の病態進行メカニズムの解明、２）M. bovis浸潤農場での易感染個体把握システムおよび発症個体の病態診断システムの開発を行い、最終的に、M. bovis浸潤農場における牛マイコプラズマ肺炎による損耗低減化のための飼養管理技術の開発を目指す。