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Faculty of Medicine School of Medicine Department of Medical Sciences, Cardiovascular Physiology |
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Assistant Professor |
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MADHYASTHA HARISHKUMAR
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Degree 【 display / non-display 】
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Ph. D ( 2006.10 Miyazaki Medical College )
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博士（植物学） ( 1996.12 サルダル パテル大学 )
Papers 【 display / non-display 】
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Dual antibacterial and anti-inflammatory efficacy of a chitosan-chondroitin sulfate-based in-situ forming wound dressing Reviewed International coauthorship
Sharma S., Madhyastha H., Kirwale S.S., Sakai K., Katakia Y.T., Majumder S., Roy A.
Carbohydrate Polymers 298 2022.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Carbohydrate Polymers
None of the currently available wound dressings exhibit combined antibacterial and anti-inflammatory activity. Using polyelectrolyte complexation (PEC) between a cationic polysaccharide chitosan (CH) and an anionic glycosaminoglycan chondroitin sulfate (CS), we have developed a unique in-situ forming scaffold (CH-CS PEC), which develops at the wound site itself to influence the function of the wound bed cells. The current study demonstrated that CH-CS PEC could induce bacterial cell death through membrane pore formation and increased ROS production. Moreover, possibly due to its unique material properties including medium-soft viscoelasticity, porosity, and surface composition, CH-CS PEC could modulate macrophage function, increasing their phagocytic ability with low TNF-α and high IL-10 production. Faster wound closure and decreased CFU count was observed in an in-vivo infected wound model, with reduced NF-κB and increased VE-cadherin expression, indicating reduced inflammation and enhanced angiogenesis. In summary, this study exhibited that CH-CS PEC has substantial antibacterial and immunomodulatory properties.
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Mathiyazhagan J., Madhyastha H., Nalini E., Gothandam K.M.
Current Trends in Biotechnology and Pharmacy 16 56 - 63 2022.6
Language：English Publishing type：Research paper (scientific journal) Publisher：Current Trends in Biotechnology and Pharmacy
Ocimum sanctum and Piper nigrum are medicinal plants traditionally used in Ayurveda and other ancient therapeutic systems. Till today people use these plants In one of the different aspects of their everyday life. This study revealed the combined effect of O. sanctum and P. nigrum (1:1) against diabetic-related enzymes in a cell-free system and breast cancer cell line (MCF-7). The HPLC analysis revealed that Eugenol and Piperine are the major phytocompounds in O. sanctum and P. nigrum, respectively. Digestive enzymes like a- amylase and a- glucos¡dase assays were carried out to find their antidiabetic effect. To determine the Interaction between Piperine and eugenol with enzymes involved in diabetes, the in silico molecular docking analysis was performed. The plant extracts alone and in combination, were checked for their cytotoxicity and reactive oxygen species levels in MCF-7 cells.
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Chromatic intervention and biocompatibility assay for biosurfactant derived from Balanites aegyptiaca (L.) Del Reviewed International coauthorship
Panchariya V., Bhati V., Madhyastha H., Madhyastha R., Prasad J., Sharma P., Sharma P., Harish , Saini M.K., Rajput V.D., Nakajima Y., Kothari S.L., Gour V.S.
Scientific Reports 11 ( 1 ) 4186 2021.12
Language：English Publishing type：Research paper (scientific journal) Publisher：Scientific Reports
Extraction of biosurfactants from plants is advantageous than from microbes. The properties and robustness of biosurfactant derived from the mesocarp of Balanites aegyptiaca have been reported. However, the dark brown property of biosurfactant and lack of knowledge of its biocompatibility limits its scope. In the present work, the decolorization protocol for this biosurfactant was optimized using hydrogen peroxide. The hemolytic potential and biocompatibility based on cell toxicity and proliferation were also investigated. This study is the first report on the decolorization and toxicity assay of this biosurfactant. For decolorization of biosurfactant, 34 full factorial design was used, and the data were subjected to ANOVA. Results indicate that 1.5% of hydrogen peroxide can decolorize the biosurfactant most efficiently at 40 °C in 70 min at pH 7. Mitochondrial reductase (MTT) and reactive oxygen species (ROS) assays on M5S mouse skin fibroblast cells revealed that decolorized biosurfactant up to 50 µg/mL for 6 h had no significant toxic effect. Hemolysis assay showed ~ 2.5% hemolysis of human RBCs, indicating the nontoxic effect of this biosurfactant. The present work established a decolorization protocol making the biosurfactant chromatically acceptable. Biocompatibility assays confirm its safer use as observed by experiments on M5S skin fibroblast cells under in vitro conditions.
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Designer exosomes:smart nano-communications tools for translational medicine Invited Reviewed International coauthorship
Madhyastha Harishkumar, Madhyastha Radha, Nakajima Y., G.M. Muthukalainan, Ohe. K.,Shiomori K. Watanabe Nozoomi.
Bioengienraing 2021.10
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Development of an in-situ forming, self-healing scaffold for dermal wound healing: in-vitro and in-vivo studies Reviewed International coauthorship
Sharma S., Madhyastha H., Laxmi Swetha K., Maravajjala K.S., Singh A., Madhyastha R., Nakajima Y., Roy A.
Materials Science and Engineering C 128 112263 2021.9
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Materials Science and Engineering C
The importance of the extra-cellular matrix (ECM) for wound healing has been extensively researched. Understanding its importance, multiple ECM mimetic scaffolds have been developed. However, the majority of such scaffolds are prefabricated. Due to their stiffness, prefabricated scaffolds cannot come into direct contact with the basal skin cells at the wound bed, limiting their efficacy. We have developed a unique wound dressing, using chitosan (CH) and chondroitin sulfate (CS), that can form a porous scaffold (CH-CS PEC) in-situ, at the wound site, by simple mixing of the polymer solutions. As CH is positively and CS is negatively charged, mixing these two polymer solutions would lead to electrostatic cross-linking between the polymers, converting them to a porous, viscoelastic scaffold. Owing to the in-situ formation, the scaffold can come in direct contact with the cells at the wound bed, supporting their proliferation and biofunction. In the present study, we confirmed the cross-linked scaffold formation by solid-state NMR, XRD, and TGA analysis. We have demonstrated that the scaffold had a high viscoelastic property, with self-healing capability. Both keratinocyte and fibroblast cells exhibited significantly increased migration and functional markers expression when grown on this scaffold. In the rat skin-excisional wound model, treatment with the in-situ forming CH-CS PEC exhibited enhanced wound healing efficacy. Altogether, this study demonstrated that mixing CH and CS solutions lead to the spontaneous formation of a highly viscoelastic, porous scaffold, which can support epidermal and dermal cell proliferation and bio-function, with an enhanced in-vivo wound healing efficacy.
Books 【 display / non-display 】
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Nanotoxicology: Toxicity evaluation, risk assessment and management
Umapathi A, Kaphle A, Navya PN, Firdose N, Monnappa S, Jain D, Srinivas SP, Madhyastha HK, Madhyastha R, Daima HK（ Role： Joint author）
CRC Press, Taylor and Francis group 2017.5
Language：English Book type：Scholarly book
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Polymeric nanocarriers for vaccine delivery.
Shakya A K and Madhyastha Harishkumar（ Role： Joint author）
In Integrating biologically inspired nanotechnology into medical practice 2016.8
Language：English Book type：Scholarly book
Presentations 【 display / non-display 】
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Silver primed nutraceutical hybrids: cellular and hematological evaluation Invited International conference
Madhyastha H, Madhyastha r., Nakajima Y., Maruyama M.
BioTERM
Event date： 2019.11.30 - 2019.12.3
Language：English Presentation type：Oral presentation (invited, special)
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Silver primed nutraceutical hybrids: cellular and hematological evaluation Invited International conference
Madhyastha H, Madhyastha r., Nakajima Y., Maruyama M.
BioTERM
Event date： 2019.11.30 - 2019.12.3
Language：English Presentation type：Oral presentation (invited, special)
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Phycocyanin hybridized silver conjugates in wound healing-cell toxicity evaluation Invited International conference
Madhyastha H, Madhyastha R., Nakajima Y., Maruyama M.
New Horizon in Biotechnology
Event date： 2019.11.20 - 2019.11.29
Language：English Presentation type：Oral presentation (invited, special)
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Phycocyanin hybridized silver conjugates in wound healing-cell toxicity evaluation Invited International conference
Madhyastha H, Madhyastha R., Nakajima Y., Maruyama M.
New Horizon in Biotechnology
Event date： 2019.11.20 - 2019.11.29
Language：English Presentation type：Oral presentation (invited, special)
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Potential of intracellular protein and its hydrolysates from epiphytic bacteria associated with brown algae, Sargassum sp. as anticancer agents.
Nur Asmi, Ahyar Ahmad, Hasnah Natsir, Muh. Nasrum Massi, Harishkumar Madhyastha, Radha Madhyastha, Yuichi Nakajima, Masugi Maruyama.
西日本生理学会 (宮崎大学)
Event date： 2019.11.1 - 2019.11.2
Language：English Presentation type：Oral presentation (general)
Venue：宮崎大学
Grant-in-Aid for Scientific Research 【 display / non-display 】
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FMR治療における病理、遺伝子発現機構からの検証に基づいた左房機能評価の意義の確立
Grant number：23K08239 2023.04 - 2027.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
Authorship：Coinvestigator(s)