マドゥエスタ ハリーシャクマール (マドウエスタ ハリーシヤクマール)

MADHYASTHA HARISHKUMAR

写真a

所属

医学部 医学科 機能制御学講座循環動態生理学分野

職名

助教

外部リンク

学位 【 表示 / 非表示

  • 博士(医学) ( 2006年10月   宮崎医科大学 )

  • 博士(植物学) ( 1996年12月   サルダル パテル大学 )

 

論文 【 表示 / 非表示

  • Dual antibacterial and anti-inflammatory efficacy of a chitosan-chondroitin sulfate-based in-situ forming wound dressing 査読あり 国際共著

    Sharma S., Madhyastha H., Kirwale S.S., Sakai K., Katakia Y.T., Majumder S., Roy A.

    Carbohydrate Polymers   298   2022年12月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Carbohydrate Polymers  

    None of the currently available wound dressings exhibit combined antibacterial and anti-inflammatory activity. Using polyelectrolyte complexation (PEC) between a cationic polysaccharide chitosan (CH) and an anionic glycosaminoglycan chondroitin sulfate (CS), we have developed a unique in-situ forming scaffold (CH-CS PEC), which develops at the wound site itself to influence the function of the wound bed cells. The current study demonstrated that CH-CS PEC could induce bacterial cell death through membrane pore formation and increased ROS production. Moreover, possibly due to its unique material properties including medium-soft viscoelasticity, porosity, and surface composition, CH-CS PEC could modulate macrophage function, increasing their phagocytic ability with low TNF-α and high IL-10 production. Faster wound closure and decreased CFU count was observed in an in-vivo infected wound model, with reduced NF-κB and increased VE-cadherin expression, indicating reduced inflammation and enhanced angiogenesis. In summary, this study exhibited that CH-CS PEC has substantial antibacterial and immunomodulatory properties.

    DOI: 10.1016/j.carbpol.2022.120126

    Scopus

  • Synergistic Effect of Ocimum sanctum and Piper nigrum: An In Vitro Study on Type 2 Diabetes-Related Enzymes and MCF-7 Breast Cancer Cell line 査読あり

    Mathiyazhagan J., Madhyastha H., Nalini E., Gothandam K.M.

    Current Trends in Biotechnology and Pharmacy   16   56 - 63   2022年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Current Trends in Biotechnology and Pharmacy  

    Ocimum sanctum and Piper nigrum are medicinal plants traditionally used in Ayurveda and other ancient therapeutic systems. Till today people use these plants In one of the different aspects of their everyday life. This study revealed the combined effect of O. sanctum and P. nigrum (1:1) against diabetic-related enzymes in a cell-free system and breast cancer cell line (MCF-7). The HPLC analysis revealed that Eugenol and Piperine are the major phytocompounds in O. sanctum and P. nigrum, respectively. Digestive enzymes like a- amylase and a- glucos¡dase assays were carried out to find their antidiabetic effect. To determine the Interaction between Piperine and eugenol with enzymes involved in diabetes, the in silico molecular docking analysis was performed. The plant extracts alone and in combination, were checked for their cytotoxicity and reactive oxygen species levels in MCF-7 cells.

    DOI: 10.5530/ctbp.2022.2s.31

    Scopus

  • Chromatic intervention and biocompatibility assay for biosurfactant derived from Balanites aegyptiaca (L.) Del 査読あり 国際共著

    Panchariya V., Bhati V., Madhyastha H., Madhyastha R., Prasad J., Sharma P., Sharma P., Harish , Saini M.K., Rajput V.D., Nakajima Y., Kothari S.L., Gour V.S.

    Scientific Reports   11 ( 1 )   4186   2021年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Scientific Reports  

    Extraction of biosurfactants from plants is advantageous than from microbes. The properties and robustness of biosurfactant derived from the mesocarp of Balanites aegyptiaca have been reported. However, the dark brown property of biosurfactant and lack of knowledge of its biocompatibility limits its scope. In the present work, the decolorization protocol for this biosurfactant was optimized using hydrogen peroxide. The hemolytic potential and biocompatibility based on cell toxicity and proliferation were also investigated. This study is the first report on the decolorization and toxicity assay of this biosurfactant. For decolorization of biosurfactant, 34 full factorial design was used, and the data were subjected to ANOVA. Results indicate that 1.5% of hydrogen peroxide can decolorize the biosurfactant most efficiently at 40 °C in 70 min at pH 7. Mitochondrial reductase (MTT) and reactive oxygen species (ROS) assays on M5S mouse skin fibroblast cells revealed that decolorized biosurfactant up to 50 µg/mL for 6 h had no significant toxic effect. Hemolysis assay showed ~ 2.5% hemolysis of human RBCs, indicating the nontoxic effect of this biosurfactant. The present work established a decolorization protocol making the biosurfactant chromatically acceptable. Biocompatibility assays confirm its safer use as observed by experiments on M5S skin fibroblast cells under in vitro conditions.

    DOI: 10.1038/s41598-021-83573-7

    Scopus

    PubMed

  • Designer exosomes:smart nano-communications tools for translational medicine 招待あり 査読あり 国際共著

    Madhyastha Harishkumar, Madhyastha Radha, Nakajima Y., G.M. Muthukalainan, Ohe. K.,Shiomori K. Watanabe Nozoomi.

    Bioengienraing   2021年10月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Development of an in-situ forming, self-healing scaffold for dermal wound healing: in-vitro and in-vivo studies 査読あり 国際共著

    Sharma S., Madhyastha H., Laxmi Swetha K., Maravajjala K.S., Singh A., Madhyastha R., Nakajima Y., Roy A.

    Materials Science and Engineering C   128   112263   2021年9月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Materials Science and Engineering C  

    The importance of the extra-cellular matrix (ECM) for wound healing has been extensively researched. Understanding its importance, multiple ECM mimetic scaffolds have been developed. However, the majority of such scaffolds are prefabricated. Due to their stiffness, prefabricated scaffolds cannot come into direct contact with the basal skin cells at the wound bed, limiting their efficacy. We have developed a unique wound dressing, using chitosan (CH) and chondroitin sulfate (CS), that can form a porous scaffold (CH-CS PEC) in-situ, at the wound site, by simple mixing of the polymer solutions. As CH is positively and CS is negatively charged, mixing these two polymer solutions would lead to electrostatic cross-linking between the polymers, converting them to a porous, viscoelastic scaffold. Owing to the in-situ formation, the scaffold can come in direct contact with the cells at the wound bed, supporting their proliferation and biofunction. In the present study, we confirmed the cross-linked scaffold formation by solid-state NMR, XRD, and TGA analysis. We have demonstrated that the scaffold had a high viscoelastic property, with self-healing capability. Both keratinocyte and fibroblast cells exhibited significantly increased migration and functional markers expression when grown on this scaffold. In the rat skin-excisional wound model, treatment with the in-situ forming CH-CS PEC exhibited enhanced wound healing efficacy. Altogether, this study demonstrated that mixing CH and CS solutions lead to the spontaneous formation of a highly viscoelastic, porous scaffold, which can support epidermal and dermal cell proliferation and bio-function, with an enhanced in-vivo wound healing efficacy.

    DOI: 10.1016/j.msec.2021.112263

    Scopus

    PubMed

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書籍等出版物 【 表示 / 非表示

  • Nanotoxicology: Toxicity evaluation, risk assessment and management

    Umapathi A, Kaphle A, Navya PN, Firdose N, Monnappa S, Jain D, Srinivas SP, Madhyastha HK, Madhyastha R, Daima HK( 担当: 共著)

    CRC Press, Taylor and Francis group  2017年5月 

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    記述言語:英語 著書種別:学術書

  • Polymeric nanocarriers for vaccine delivery.

    Shakya A K and Madhyastha Harishkumar( 担当: 共著)

    In Integrating biologically inspired nanotechnology into medical practice  2016年8月 

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    記述言語:英語 著書種別:学術書

講演・口頭発表等 【 表示 / 非表示

  • Silver primed nutraceutical hybrids: cellular and hematological evaluation 招待あり 国際会議

    Madhyastha H, Madhyastha r., Nakajima Y., Maruyama M.

    BioTERM 

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    開催年月日: 2019年11月30日 - 2019年12月3日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

  • Silver primed nutraceutical hybrids: cellular and hematological evaluation 招待あり 国際会議

    Madhyastha H, Madhyastha r., Nakajima Y., Maruyama M.

    BioTERM 

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    開催年月日: 2019年11月30日 - 2019年12月3日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

  • Phycocyanin hybridized silver conjugates in wound healing-cell toxicity evaluation 招待あり 国際会議

    Madhyastha H, Madhyastha R., Nakajima Y., Maruyama M.

    New Horizon in Biotechnology 

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    開催年月日: 2019年11月20日 - 2019年11月29日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

  • Phycocyanin hybridized silver conjugates in wound healing-cell toxicity evaluation 招待あり 国際会議

    Madhyastha H, Madhyastha R., Nakajima Y., Maruyama M.

    New Horizon in Biotechnology 

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    開催年月日: 2019年11月20日 - 2019年11月29日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

  • Potential of intracellular protein and its hydrolysates from epiphytic bacteria associated with brown algae, Sargassum sp. as anticancer agents.

    Nur Asmi, Ahyar Ahmad, Hasnah Natsir, Muh. Nasrum Massi, Harishkumar Madhyastha, Radha Madhyastha, Yuichi Nakajima, Masugi Maruyama.

    西日本生理学会  (宮崎大学) 

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    開催年月日: 2019年11月1日 - 2019年11月2日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:宮崎大学  

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科研費(文科省・学振・厚労省)獲得実績 【 表示 / 非表示

  • FMR治療における病理、遺伝子発現機構からの検証に基づいた左房機能評価の意義の確立

    研究課題/領域番号:23K08239  2023年04月 - 2027年03月

    独立行政法人日本学術振興会  科学研究費補助金  基盤研究(C)

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    担当区分:研究分担者 

 

授業 【 表示 / 非表示

  • 環境と生命

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    科目区分:共通教育科目