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Faculty of Agriculture Department of Veterinary Science |
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Professor |
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IDA Takanori
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Research Areas 【 display / non-display 】
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Life Science / Veterinary medical science
Papers 【 display / non-display 】
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Ghrelin is essential for lowering blood pressure during torpor Reviewed
Matsui K., Ida T., Oishi K., Kojima M., Sato T.
Frontiers in Endocrinology 15 1487028 2024.10
Language：English Publishing type：Research paper (scientific journal) Publisher：Frontiers in Endocrinology
Introduction: Daily torpor is an active hypothermic phenomenon that is observed in some mammals and birds during fasting. A decrease in blood pressure has also been observed in torpor; however, there remains a lack of knowledge of the underlying mechanism. We have previously reported that ghrelin, an orexigenic hormone, has a hypothermic effect and is essential for the induction and maintenance of torpor. It is also known that the ghrelin secretion is enhanced during fasting and that ghrelin receptors are distributed in the cardiovascular system. Therefore, this study was conducted to test the hypothesis that ghrelin is actively involved in the regulation of blood pressure during torpor induction. Methods: Male wild-type and ghrelin gene-deficient mice were generated by homologous recombination as previously reported. Mice, 10 weeks old, were included in this study and housed five per cage. The mice were maintained on a 12-h light/dark cycle (lights on from 7:00 to 19:00) with access to food and water ad libitum. Results: The continuous measurement of blood pressure using a telemetry system showed that induction of torpor by fasting did not decrease blood pressure in ghrelin gene-deficient mice. The analysis of heart rate variability revealed that sympathetic nerve activity was predominant in ghrelin-deficient mice during fasting. Furthermore, these features were cancelled by administration of a ghrelin receptor agonist and were comparable to those in wild-type mice. Discussion: In this study, we showed that blood pressure was elevated in ghrl-/- mice and that the blood pressure rhythm was abnormal. Furthermore, we showed that the ghrelin gene deficiency does not cause sufficient blood pressure reduction upon entry into the torpor, and that the administration of the ghrelin receptor agonist, GHRP-6, causes blood pressure reduction associated with torpor. Thus, we have shown for the first time that the active role of ghrelin is essential for active blood pressure reduction associated with torpor, and that this action is mediated by the inhibition of sympathetic nerve activity by ghrelin.
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Yazawa T., Watanabe Y., Yokohama Y., Imamichi Y., Hasegawa K., Nakajima K.I., Kitano T., Ida T., Sato T., Islam M.S., Umezawa A., Takahashi S., Kato Y., Jahan S., Kawabe J.I.
Frontiers in Endocrinology 15 1480722 2024.10
Language：English Publishing type：Research paper (scientific journal) Publisher：Frontiers in Endocrinology
3β-Hydroxysteroid dehydrogenases (3β-HSDs) catalyze the oxidative conversion of delta (5)-ene-3-beta-hydroxy steroids and ketosteroids. Human 3β-HSD type 2 (HSD3B2) is predominantly expressed in gonadal and adrenal steroidogenic cells for producing all classes of active steroid hormones. Mutations in HSD3B2 gene cause a rare form of congenital adrenal hyperplasia with varying degree of salt wasting and incomplete masculinization, resulting from reduced production of corticoids and androgens. Therefore, evaluation of the HSD3B2 enzymatic activity in both pathways for each steroid hormone production is important for accurately understanding and diagnosing this disorder. Using progesterone receptor (PR)- and androgen receptor (AR)-mediated transactivation, we adapted a method that easily evaluates enzymatic activity of HSD3B2 by quantifying the conversion from substrates [pregnenolone (P5) and dehydroepiandrosterone (DHEA)] to (progesterone and androstenedione). HEK293 cells were transduced to express human HSD3B2, and incubated medium containing P5 or DHEA. Depending on the incubation time with HSD3B2-expressing cells, the culture media progressively increased luciferase activities in CV-1 cells, transfected with the PR/AR expression vector and progesterone-/androgen-responsive reporter. Culture media from human and other mammalian HSD3B1-expressing cells also increased the luciferase activities. HEK293 cells expressing various missense mutations in the HSD3B2 gene revealed the potential of this system to evaluate the relationship between the enzymatic activities of mutant proteins and patient phenotype.
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Acyl modifications in bovine, porcine, and equine ghrelins Reviewed
Ida T., Tominaga H., Iwamoto E., Kurogi A., Okura A., Shimada K., Kato J., Kuwano A., Ode H., Nagata S., Kitamura K., Yazawa T., Sato-Hashimoto M., Yasuda M., Miyazato M., Shiimura Y., Sato T., Kojima M.
Frontiers in Endocrinology 15 1411483 2024.5
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Frontiers in Endocrinology
Ghrelin is a peptide hormone with various important physiological functions. The unique feature of ghrelin is its serine 3 acyl-modification, which is essential for ghrelin activity. The major form of ghrelin is modified with n-octanoic acid (C8:0) by ghrelin O-acyltransferase. Various acyl modifications have been reported in different species. However, the underlying mechanism by which ghrelin is modified with various fatty acids remains to be elucidated. Herein, we report the purification of bovine, porcine, and equine ghrelins. The major active form of bovine ghrelin was a 27-amino acid peptide with an n-octanoyl (C8:0) modification at Ser3. The major active form of porcine and equine ghrelin was a 28-amino acid peptide. However, porcine ghrelin was modified with n-octanol (C8:0), whereas equine ghrelin was modified with n-butanol (C4:0) at Ser3. This study indicates the existence of structural divergence in ghrelin and suggests that it is necessary to measure the minor and major forms of ghrelin to fully understand its physiology.
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Jiang D, Matsuzaki M, Ida T, Kitamura K, Kato J
Hypertension research : official journal of the Japanese Society of Hypertension 47 ( 4 ) 1017 - 1023 2024.2
Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Nature
Increased blood pressure variability (BPV) was shown to be associated with cardiovascular morbidities and/or mortalities. There are various types of BPV depending on time intervals of BP measurements, ranging from beat-to-beat to visit-to-visit or year-to-year. We previously found that continuous infusion of noradrenaline (NA) for 14 days increased short-term BPV every 15 min in rats. The aims of this study were to examine (1) whether NA infusion increases very short-term beat-to-beat BPV, (2) the effects of azelnidipine and hydralazine on NA-induced BPV, and (3) whether baroreceptor reflex sensitivity (BRS) is affected by NA or NA plus those vasodilators. Nine-week-old Wistar rats infused subcutaneously with 30 μg/h NA were orally treated with or without 9.7 mg/day azelnidipine or 5.9 mg/day hydralazine over 14 days. BP levels were continuously monitored via abdominal aortic catheter with a telemetry system in an unrestrained condition. Standard deviations (SDs) were used to evaluate beat-to-beat BPV and BPV every 15 min which was obtained by averaging BP levels for 10-s segment at each time point. BRS was determined by a sequence analysis. Continuous NA infusion over 14 days increased average BP, beat-to-beat BPV, and BPV every 15 min, lowering BRS. Comparing the two vasodilators, hydralazine reduced BP elevation by NA; meanwhile, azelnidipine alleviated BPV augmentation, preserving BRS, despite a smaller BP reduction. Thus, NA infusion increased both very short- and short-term BPV concomitantly with impaired BRS, while azelnidipine had an inhibitory effect, possibly independent of BP-lowering, on those types of BPV and impairment of BRS.
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Diversity of Androgens; Comparison of Their Significance and Characteristics in Vertebrate Species Reviewed
Yazawa T., Imamichi Y., Sato T., Ida T., Umezawa A., Kitano T.
Zoological Science 41 ( 1 ) 77 - 86 2024.1
Language：English Publishing type：Research paper (scientific journal) Publisher：Zoological Science
Androgen(s) is one of the sex steroids that are involved in many physiological phenomena of vertebrate species. Although androgens were originally identified as male sex hormones, it is well known now that they are also essential in females. As in the case of other steroid hormones, androgen is produced from cholesterol through serial enzymatic reactions. Although testis is a major tissue to produce androgens in all species, androgens are also produced in ovary and adrenal (interrenal tissue). Testosterone is the most common and famous androgen. It represents a major androgen both in males and females of almost vertebrate species. In addition, testosterone is a precursorforproducingsignificantandrogenssuchas11-ketotestosterone,5α-dihydrotestosterone, 11-ketodihydrotestosterones and 15α-hydroxytestosterone in a species- or sex-dependent manner for their homeostasis. In this article, we will review the significance and characteristics of these androgens, following a description of the history of testosterone discovery and its synthetic pathways.
DOI： 10.2108/zs230064
MISC 【 display / non-display 】
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Discovery of feeding regulatory peptides and the importance of peptide discovery research Reviewed
Ida T, Matsui K, Nagata S, Nakamachi T, Shiimura Y, Sato T, Kojima M.
The Kurume Medical Journal 2025.3
Authorship：Lead author, Corresponding author Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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消化管関連ペプチドが拓く恒常性フロンティア Invited
佐藤貴弘、井田隆徳、関口俊男、中町智哉、児島将康
実験医学 37 ( 3 ) 1 - 8 2019
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
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Role of biological rhythms in the performance of physical activity Invited Reviewed
Sato T, Ida T, Kojima M
The Journal of Physical Fitness and Sports Medicine 6 ( 3 ) 125 - 134 2017.3
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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モデル生物を利用した新規摂食調節ペプチドの探索
井田隆徳
生物科学 2016.3
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：農山漁村文化協会
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Sano H., Nakamura A., Texada M., Truman J., Ishimoto H., Kamikouchi A., Nibu Y., Kume K., Ida T., Kojima M.
PLoS Genetics 11 ( 9 ) 2015.9
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution) Publisher：PLoS Genetics
Presentations 【 display / non-display 】
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動物介在活動から考えるOne Health Invited
井田隆徳
第１６６回日本獣医学会学術集会
Event date： 2023.9.5
Presentation type：Oral presentation (general)
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新規生理活性ペプチドの探索と応用 Invited
井田隆徳
第70回日本心臓病学会学術集会 2022.9.23
Event date： 2022.9.23
Language：Japanese Presentation type：Oral presentation (general)
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新しい生理活性ペプチドの発見に向けて Invited
井田隆徳
第143回宮崎大学農学部獣医学科集談会
Event date： 2025.3.14
Language：Japanese Presentation type：Oral presentation (general)
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ノルアドレナリン（NA）持続投与ラットにおけるナトリウム利尿ペプチド（NPs）の心肥大増強作用
姜丹鳳、松﨑美南、北村和雄、鶴田敏博、海北幸一、井田隆徳
第28回日本心血管内分泌代謝学会学術総会 2024.12.7
Event date： 2024.12.7
Presentation type：Oral presentation (general)
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血圧リズムに及ぼすグレリン作用の解析
佐藤貴弘、井田隆徳、松井一真、大石佳苗、児島将康
第28回日本心血管内分泌代謝学会学術総会
Event date： 2024.12.7
Presentation type：Oral presentation (general)
Awards 【 display / non-display 】
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日本獣医学会生理学・生化学分科会奨励賞
2010.9 日本獣医学会
井田隆徳
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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11th International Conference on Human-Animal Interactions.Best Poster Award
2007.10 IAHAIO
Yoshidomi Y, Ikeda Y, Satoh H, Sekino R, Ida T, Hisatomi I, Nakatsuka K, Misawa N
Award type：Award from international society, conference, symposium, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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植物の耐寒性を増強する低温センシング機構の解明
Grant number：24K01683 2024.04 - 2028.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(B)
Authorship：Coinvestigator(s)
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運動によって生体内で産生される匂い物質の器官嗅覚受容体を介した生理作用の解明
Grant number：24K22277 2024.04 - 2027.03
独立行政法人日本学術振興会 科学研究費基金 挑戦的研究（萌芽）
Authorship：Coinvestigator(s)
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急速減量による急性腎障害発症メカニズムの解明とその抑制法の基盤研究
Grant number：23K27975 2024.04 - 2027.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(B)
Authorship：Coinvestigator(s)
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摂食・エネルギー代謝を制御する新規生理活性ペプチドの発見と応用
Grant number：21K05958 2021.04 - 2025.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
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生体必須の金属補因子「鉄硫黄クラスター」の生合成機序と無細胞構築系の確立
Grant number：20H03196 2020.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
Authorship：Coinvestigator(s)
Other research activities 【 display / non-display 】
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テニュアトラック トロイカサポーター
2025.01
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食鳥処理施設での骨髄のサンプリング
2023.11
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食鳥処理施設での脳のサンプリング
2019.07
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食鳥処理施設での肝臓のサンプリング
2018.06
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食肉処理施設における膵臓のサンプリング
2017.07
Available Technology 【 display / non-display 】
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新規生理活性ペプチドの探索
新規生理活性ペプチドの機能解析
病態モデル生物の作製Related fields where technical consultation is available：ペプチド抽出
受容体発現細胞を用いたセカンドメッセンジャーアッセイMessage：宮崎大学の伝統あるペプチド研究を引き継ぎ、ペプチド研究におけるプラットフォームを目指しています。