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農学部 獣医学科 |
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Ghrelin is essential for lowering blood pressure during torpor 査読あり
Matsui K., Ida T., Oishi K., Kojima M., Sato T.
Frontiers in Endocrinology 15 1487028 2024年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Frontiers in Endocrinology
Introduction: Daily torpor is an active hypothermic phenomenon that is observed in some mammals and birds during fasting. A decrease in blood pressure has also been observed in torpor; however, there remains a lack of knowledge of the underlying mechanism. We have previously reported that ghrelin, an orexigenic hormone, has a hypothermic effect and is essential for the induction and maintenance of torpor. It is also known that the ghrelin secretion is enhanced during fasting and that ghrelin receptors are distributed in the cardiovascular system. Therefore, this study was conducted to test the hypothesis that ghrelin is actively involved in the regulation of blood pressure during torpor induction. Methods: Male wild-type and ghrelin gene-deficient mice were generated by homologous recombination as previously reported. Mice, 10 weeks old, were included in this study and housed five per cage. The mice were maintained on a 12-h light/dark cycle (lights on from 7:00 to 19:00) with access to food and water ad libitum. Results: The continuous measurement of blood pressure using a telemetry system showed that induction of torpor by fasting did not decrease blood pressure in ghrelin gene-deficient mice. The analysis of heart rate variability revealed that sympathetic nerve activity was predominant in ghrelin-deficient mice during fasting. Furthermore, these features were cancelled by administration of a ghrelin receptor agonist and were comparable to those in wild-type mice. Discussion: In this study, we showed that blood pressure was elevated in ghrl-/- mice and that the blood pressure rhythm was abnormal. Furthermore, we showed that the ghrelin gene deficiency does not cause sufficient blood pressure reduction upon entry into the torpor, and that the administration of the ghrelin receptor agonist, GHRP-6, causes blood pressure reduction associated with torpor. Thus, we have shown for the first time that the active role of ghrelin is essential for active blood pressure reduction associated with torpor, and that this action is mediated by the inhibition of sympathetic nerve activity by ghrelin.
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Yazawa T., Watanabe Y., Yokohama Y., Imamichi Y., Hasegawa K., Nakajima K.I., Kitano T., Ida T., Sato T., Islam M.S., Umezawa A., Takahashi S., Kato Y., Jahan S., Kawabe J.I.
Frontiers in Endocrinology 15 1480722 2024年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Frontiers in Endocrinology
3β-Hydroxysteroid dehydrogenases (3β-HSDs) catalyze the oxidative conversion of delta (5)-ene-3-beta-hydroxy steroids and ketosteroids. Human 3β-HSD type 2 (HSD3B2) is predominantly expressed in gonadal and adrenal steroidogenic cells for producing all classes of active steroid hormones. Mutations in HSD3B2 gene cause a rare form of congenital adrenal hyperplasia with varying degree of salt wasting and incomplete masculinization, resulting from reduced production of corticoids and androgens. Therefore, evaluation of the HSD3B2 enzymatic activity in both pathways for each steroid hormone production is important for accurately understanding and diagnosing this disorder. Using progesterone receptor (PR)- and androgen receptor (AR)-mediated transactivation, we adapted a method that easily evaluates enzymatic activity of HSD3B2 by quantifying the conversion from substrates [pregnenolone (P5) and dehydroepiandrosterone (DHEA)] to (progesterone and androstenedione). HEK293 cells were transduced to express human HSD3B2, and incubated medium containing P5 or DHEA. Depending on the incubation time with HSD3B2-expressing cells, the culture media progressively increased luciferase activities in CV-1 cells, transfected with the PR/AR expression vector and progesterone-/androgen-responsive reporter. Culture media from human and other mammalian HSD3B1-expressing cells also increased the luciferase activities. HEK293 cells expressing various missense mutations in the HSD3B2 gene revealed the potential of this system to evaluate the relationship between the enzymatic activities of mutant proteins and patient phenotype.
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Acyl modifications in bovine, porcine, and equine ghrelins 査読あり
Ida T., Tominaga H., Iwamoto E., Kurogi A., Okura A., Shimada K., Kato J., Kuwano A., Ode H., Nagata S., Kitamura K., Yazawa T., Sato-Hashimoto M., Yasuda M., Miyazato M., Shiimura Y., Sato T., Kojima M.
Frontiers in Endocrinology 15 1411483 2024年5月
担当区分：筆頭著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Frontiers in Endocrinology
Ghrelin is a peptide hormone with various important physiological functions. The unique feature of ghrelin is its serine 3 acyl-modification, which is essential for ghrelin activity. The major form of ghrelin is modified with n-octanoic acid (C8:0) by ghrelin O-acyltransferase. Various acyl modifications have been reported in different species. However, the underlying mechanism by which ghrelin is modified with various fatty acids remains to be elucidated. Herein, we report the purification of bovine, porcine, and equine ghrelins. The major active form of bovine ghrelin was a 27-amino acid peptide with an n-octanoyl (C8:0) modification at Ser3. The major active form of porcine and equine ghrelin was a 28-amino acid peptide. However, porcine ghrelin was modified with n-octanol (C8:0), whereas equine ghrelin was modified with n-butanol (C4:0) at Ser3. This study indicates the existence of structural divergence in ghrelin and suggests that it is necessary to measure the minor and major forms of ghrelin to fully understand its physiology.
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Jiang D, Matsuzaki M, Ida T, Kitamura K, Kato J
Hypertension research : official journal of the Japanese Society of Hypertension 47 ( 4 ) 1017 - 1023 2024年2月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Springer Nature
Increased blood pressure variability (BPV) was shown to be associated with cardiovascular morbidities and/or mortalities. There are various types of BPV depending on time intervals of BP measurements, ranging from beat-to-beat to visit-to-visit or year-to-year. We previously found that continuous infusion of noradrenaline (NA) for 14 days increased short-term BPV every 15 min in rats. The aims of this study were to examine (1) whether NA infusion increases very short-term beat-to-beat BPV, (2) the effects of azelnidipine and hydralazine on NA-induced BPV, and (3) whether baroreceptor reflex sensitivity (BRS) is affected by NA or NA plus those vasodilators. Nine-week-old Wistar rats infused subcutaneously with 30 μg/h NA were orally treated with or without 9.7 mg/day azelnidipine or 5.9 mg/day hydralazine over 14 days. BP levels were continuously monitored via abdominal aortic catheter with a telemetry system in an unrestrained condition. Standard deviations (SDs) were used to evaluate beat-to-beat BPV and BPV every 15 min which was obtained by averaging BP levels for 10-s segment at each time point. BRS was determined by a sequence analysis. Continuous NA infusion over 14 days increased average BP, beat-to-beat BPV, and BPV every 15 min, lowering BRS. Comparing the two vasodilators, hydralazine reduced BP elevation by NA; meanwhile, azelnidipine alleviated BPV augmentation, preserving BRS, despite a smaller BP reduction. Thus, NA infusion increased both very short- and short-term BPV concomitantly with impaired BRS, while azelnidipine had an inhibitory effect, possibly independent of BP-lowering, on those types of BPV and impairment of BRS.
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Diversity of Androgens; Comparison of Their Significance and Characteristics in Vertebrate Species 査読あり
Yazawa T., Imamichi Y., Sato T., Ida T., Umezawa A., Kitano T.
Zoological Science 41 ( 1 ) 77 - 86 2024年1月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Zoological Science
Androgen(s) is one of the sex steroids that are involved in many physiological phenomena of vertebrate species. Although androgens were originally identified as male sex hormones, it is well known now that they are also essential in females. As in the case of other steroid hormones, androgen is produced from cholesterol through serial enzymatic reactions. Although testis is a major tissue to produce androgens in all species, androgens are also produced in ovary and adrenal (interrenal tissue). Testosterone is the most common and famous androgen. It represents a major androgen both in males and females of almost vertebrate species. In addition, testosterone is a precursorforproducingsignificantandrogenssuchas11-ketotestosterone,5α-dihydrotestosterone, 11-ketodihydrotestosterones and 15α-hydroxytestosterone in a species- or sex-dependent manner for their homeostasis. In this article, we will review the significance and characteristics of these androgens, following a description of the history of testosterone discovery and its synthetic pathways.
DOI： 10.2108/zs230064
MISC 【 表示 ／ 非表示 】
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Discovery of feeding regulatory peptides and the importance of peptide discovery research 査読あり
Ida T, Matsui K, Nagata S, Nakamachi T, Shiimura Y, Sato T, Kojima M.
The Kurume Medical Journal 2025年3月
担当区分：筆頭著者, 責任著者 記述言語：英語 掲載種別：記事・総説・解説・論説等（学術雑誌）
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消化管関連ペプチドが拓く恒常性フロンティア 招待あり
佐藤貴弘、井田隆徳、関口俊男、中町智哉、児島将康
実験医学 37 ( 3 ) 1 - 8 2019年
記述言語：日本語 掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）
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Role of biological rhythms in the performance of physical activity 招待あり 査読あり
Sato T, Ida T, Kojima M
The Journal of Physical Fitness and Sports Medicine 6 ( 3 ) 125 - 134 2017年3月
記述言語：英語 掲載種別：記事・総説・解説・論説等（学術雑誌）
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モデル生物を利用した新規摂食調節ペプチドの探索
井田隆徳
生物科学 2016年3月
記述言語：日本語 掲載種別：記事・総説・解説・論説等（学術雑誌） 出版者・発行元：農山漁村文化協会
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Sano H., Nakamura A., Texada M., Truman J., Ishimoto H., Kamikouchi A., Nibu Y., Kume K., Ida T., Kojima M.
PLoS Genetics 11 ( 9 ) 2015年9月
講演・口頭発表等 【 表示 ／ 非表示 】
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動物介在活動から考えるOne Health 招待あり
井田隆徳
第１６６回日本獣医学会学術集会
開催年月日： 2023年9月5日
会議種別：口頭発表（一般）
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新規生理活性ペプチドの探索と応用 招待あり
井田隆徳
第70回日本心臓病学会学術集会 2022年9月23日
開催年月日： 2022年9月23日
記述言語：日本語 会議種別：口頭発表（一般）
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新しい生理活性ペプチドの発見に向けて 招待あり
井田隆徳
第143回宮崎大学農学部獣医学科集談会
開催年月日： 2025年3月14日
記述言語：日本語 会議種別：口頭発表（一般）
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ノルアドレナリン（NA）持続投与ラットにおけるナトリウム利尿ペプチド（NPs）の心肥大増強作用
姜丹鳳、松﨑美南、北村和雄、鶴田敏博、海北幸一、井田隆徳
第28回日本心血管内分泌代謝学会学術総会 2024年12月7日
開催年月日： 2024年12月7日
会議種別：口頭発表（一般）
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血圧リズムに及ぼすグレリン作用の解析
佐藤貴弘、井田隆徳、松井一真、大石佳苗、児島将康
第28回日本心血管内分泌代謝学会学術総会
開催年月日： 2024年12月7日
会議種別：口頭発表（一般）
受賞 【 表示 ／ 非表示 】
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日本獣医学会生理学・生化学分科会奨励賞
2010年9月 日本獣医学会
井田隆徳
受賞区分：国内学会・会議・シンポジウム等の賞 受賞国：日本国
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11th International Conference on Human-Animal Interactions.Best Poster Award
2007年10月 IAHAIO
Yoshidomi Y, Ikeda Y, Satoh H, Sekino R, Ida T, Hisatomi I, Nakatsuka K, Misawa N
受賞区分：国際学会・会議・シンポジウム等の賞 受賞国：日本国
科研費（文科省・学振・厚労省）獲得実績 【 表示 ／ 非表示 】
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植物の耐寒性を増強する低温センシング機構の解明
研究課題/領域番号：24K01683 2024年04月 - 2028年03月
独立行政法人日本学術振興会 科学研究費基金 基盤研究(B)
担当区分：研究分担者
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運動によって生体内で産生される匂い物質の器官嗅覚受容体を介した生理作用の解明
研究課題/領域番号：24K22277 2024年04月 - 2027年03月
独立行政法人日本学術振興会 科学研究費基金 挑戦的研究（萌芽）
担当区分：研究分担者
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急速減量による急性腎障害発症メカニズムの解明とその抑制法の基盤研究
研究課題/領域番号：23K27975 2024年04月 - 2027年03月
独立行政法人日本学術振興会 科学研究費基金 基盤研究(B)
担当区分：研究分担者
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摂食・エネルギー代謝を制御する新規生理活性ペプチドの発見と応用
研究課題/領域番号：21K05958 2021年04月 - 2025年03月
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
担当区分：研究代表者
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生体必須の金属補因子「鉄硫黄クラスター」の生合成機序と無細胞構築系の確立
研究課題/領域番号：20H03196 2020年04月 - 2024年03月
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
担当区分：研究分担者
その他競争的資金獲得実績 【 表示 ／ 非表示 】
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新規生理活性ペプチドの探索
2010年01月 - 2015年03月
文部科学省 特色ある大学教育支援プログラム
担当区分：研究代表者 資金種別：競争的資金
新規生理活性ペプチドの探索
その他研究活動 【 表示 ／ 非表示 】
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テニュアトラック トロイカサポーター
2025年01月 - 現在
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食鳥処理施設での骨髄のサンプリング
2023年11月
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食鳥処理施設での脳のサンプリング
2019年07月
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食鳥処理施設での肝臓のサンプリング
2018年06月
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食肉処理施設における膵臓のサンプリング
2017年07月
研究・技術シーズ 【 表示 ／ 非表示 】
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新規生理活性ペプチドの探索
新規生理活性ペプチドの機能解析
病態モデル生物の作製技術相談に応じられる関連分野：ペプチド抽出
受容体発現細胞を用いたセカンドメッセンジャーアッセイメッセージ：宮崎大学の伝統あるペプチド研究を引き継ぎ、ペプチド研究におけるプラットフォームを目指しています。