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フロンティア科学総合研究センター 生理活性物質研究部門生理活性物質探索病態解析分野 |
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准教授 |
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論文 【 表示 ／ 非表示 】
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Insights into the Regulation of Offspring Growth by Maternally Derived Ghrelin 査読あり
Sato T, Ida T, Shimura Y, IMatsui K, Oishi K, Kojima M
Frontiers in Endocrinology 13 852636 2022年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Frontiers in Endocrinology
The regulation of fetal development by bioactive substances such as hormones and neuropeptides derived from the gestational mother is considered to be essential for the development of the fetus. On the other hand, it has been suggested that changes in the physiological state of the pregnant mother due to various factors may alter the secretion of these bioactive substances and induce metabolic changes in the offspring, such as obesity, overeating, and inflammation, thereby affecting postnatal growth and health. However, our knowledge of how gestational maternal bioactive substances modulate offspring physiology remains fragmented and lacks a systematic understanding. In this mini-review, we focus on ghrelin, which regulates growth and energy metabolism, to advance our understanding of the mechanisms by which maternally derived ghrelin regulates the growth and health of the offspring. Understanding the regulation of offspring growth by maternally-derived ghrelin is expected to clarify the fetal onset of metabolic abnormalities and lead to a better understanding of lifelong health in the next generation of offspring.
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Thermoregulatory role of ghrelin in the induction of torpor under a restricted feeding condition 査読あり
Sato T., Oishi K., Koga D., Ida T., Sakai Y., Kangawa K., Kojima M.
Scientific Reports 11 ( 1 ) 17954 2021年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Scientific Reports
Ghrelin, a circulating orexigenic hormone secreted from the stomach, stimulates appetite and food intake by activating the hypothalamic arcuate nucleus. Administration of exogenous ghrelin exerts anabolic effects, causing weight gain, increased adiposity, and decreased metabolism. Body temperature (BT), which is determined by the balance of heat production and heat loss, must be strictly regulated to maintain proper cellular function and metabolism. However, the role of ghrelin in thermoregulation remains unclear. In this study, we found that ghrelin was essential for decreasing BT when mice are placed under calorie restriction. Elevated ghrelin concentrations induced by fasting correlated with significant decreases in BT, a hibernation-like state called torpor. Ghrelin-deficient (Ghrl−/−) animals could not enter torpor. The BT of Ghrl−/− mice also remained high under restricted feeding, but the animals gradually entered precipitous hypothermia, indicating thermoregulatory impairment. These effects of ghrelin on thermoregulation were the result of suppression of sympathetic nervous system activity input to brown adipose tissue; in the absence of ghrelin, it was not possible to suppress uncoupling protein 1 (ucp1) expression and decrease BT in low-energy states. Together, these findings demonstrate that ghrelin is an essential circulating hormone involved in lowering BT.
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Analyses of molecular characteristics and enzymatic activities of ovine hsd17b3 査読あり
Islam M.S., Uwada J., Hayashi J., Kikuya K.I., Muranishi Y., Watanabe H., Yaegashi K., Hasegawa K., Ida T., Sato T., Imamichi Y., Kitano T., Miyashiro Y., Khan R.I., Takahashi S., Umezawa A., Suzuki N., Sekiguchi T., Yazawa T.
Animals 11 ( 10 ) 2021年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Animals
17β-hydroxysteroid dehydrogenase type 3 (HSD17B3) converts androstenedione (A4) into testosterone (T), which regulates sex steroid production. Because various mutations of the HSD17B3 gene cause disorder of sex differentiation (DSD) in multiple mammalian species, it is very important to reveal the molecular characteristics of this gene in various species. Here, we revealed the open reading frame of the ovine HSD17B3 gene. Enzymatic activities of ovine HSD17B3 and HSD17B1 for converting A4 to T were detected using ovine androgen receptor-mediated transactivation in reporter assays. Although HSD17B3 also converted estrone to estradiol, this activity was much weaker than those of HSD17B1. Although ovine HSD17B3 has an amino acid sequence that is conserved compared with other mammalian species, it possesses two amino acid substitutions that are consistent with the reported variants of human HSD17B3. Substitutions of these amino acids in ovine HSD17B3 for those in human did not affect the enzymatic activities. However, enzymatic activities declined upon missense mutations of the HSD17B3 gene associated with 46,XY DSD, affecting amino acids that are conserved between these two species. The present study provides basic information and tools to investigate the molecular mechanisms behind DSD not only in ovine, but also in various mammalian species.
DOI： 10.3390/ani11102876
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Kato J., Kawagoe Y., Jiang D., Ida T., Shimamoto S., Igarashi K., Kitamura K.
Peptides 142 2021年8月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Peptides
Plasma levels of the hypotensive peptides of adrenomedullin and atrial and B-type natriuretic peptides (AM, ANP, BNP) are possible biomarkers for cardiovascular diseases. Increased variability of body mass index (BMI) over a certain period of time has been reported to be associated with cardiovascular morbidity or mortality. The aim of this study is to examine clinical significance of those hypotensive peptides as biomarkers by analyzing the relationship between plasma levels of the peptides and year-by-year variability of BMI in the general population without overt cardiovascular diseases. The subjects were 427 local residents (141 males and 286 females) attending their annual health check-up, who had been examined at least 5 times over the preceding period of 10 years. They were divided into two groups of low or high variability by the median of coefficient of variation (CV) of BMI values for each gender. Plasma AM levels of those with high year-by-year variability of BMI were significantly increased, as compared to the group with low variability, in both genders; meanwhile, such a difference was not noted in plasma levels of the natriuretic peptides. No significant differences were found in the basal parameters, which could affect plasma AM level, such as age, BMI, blood pressure or serum creatinine, between two groups. In conclusion, increase in plasma AM was associated with high year-by-year variability of BMI in the general population without overt heart disease. This relationship between the two suggests that increased plasma AM level is a cardiovascular risk marker.
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Characterization of putative tachykinin peptides in Caenorhabditis elegans 査読あり
Sakai N., Ohno H., Yoshida M., Iwamoto E., Kurogi A., Jiang D., Sato T., Miyazato M., Kojima M., Kato J., Ida T.
Biochemical and Biophysical Research Communications 559 197 - 202 2021年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Biochemical and Biophysical Research Communications
Tachykinin-like peptides, such as substance P, neurokinin A, and neurokinin B, are among the earliest discovered and best-studied neuropeptide families, and research on them has contributed greatly to our understanding of the endocrine control of many physiological processes. However, there are still many orphan tachykinin receptor homologs for which cognate ligands have not yet been identified, especially in small invertebrates, such as the nematode Caenorhabditis elegans (C. elegans). We here show that the C. elegans nlp-58 gene encodes putative ligands for the orphan G protein-coupled receptor (GPCR) TKR-1, which is a worm ortholog of tachykinin receptors. We first determine, through an unbiased biochemical screen, that a peptide derived from the NLP-58 preprotein stimulates TKR-1. Three mature peptides that are predicted to be generated from NLP-58 show potent agonist activity against TKR-1. We designate these peptides as C. elegans tachykinin (CeTK)-1, −2, and −3. The CeTK peptides contain the C-terminal sequence GLR-amide, which is shared by tachykinin-like peptides in other invertebrate species. nlp-58 exhibits a strongly restricted expression pattern in several neurons, implying that CeTKs behave as neuropeptides. The discovery of CeTKs provides important information to aid our understanding of tachykinin-like peptides and their functional interaction with GPCRs.
MISC 【 表示 ／ 非表示 】
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Insights into the Regulation of Offspring Growth by Maternally Derived Ghrelin
Sato T, Ida T, Shimura Y, Matsui K, Oishi K, Kojima M
Frontiers in Endocrinology 2022年
記述言語：英語 掲載種別：記事・総説・解説・論説等（学術雑誌）
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消化管関連ペプチドが拓く恒常性フロンティア 招待あり
佐藤貴弘、井田隆徳、関口俊男、中町智哉、児島将康
実験医学 37 ( 3 ) 1 - 8 2019年
記述言語：日本語 掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）
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Role of biological rhythms in the performance of physical activity 招待あり 査読あり
Sato T, Ida T, Kojima M
The Journal of Physical Fitness and Sports Medicine 6 ( 3 ) 125 - 134 2017年3月
記述言語：英語 掲載種別：記事・総説・解説・論説等（学術雑誌）
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モデル生物を利用した新規摂食調節ペプチドの探索
井田隆徳
生物科学 2016年3月
記述言語：日本語 掲載種別：記事・総説・解説・論説等（学術雑誌） 出版者・発行元：農山漁村文化協会
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Sano H., Nakamura A., Texada M., Truman J., Ishimoto H., Kamikouchi A., Nibu Y., Kume K., Ida T., Kojima M.
PLoS Genetics 11 ( 9 ) 2015年9月
講演・口頭発表等 【 表示 ／ 非表示 】
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ルシフェラーゼアッセイによるHSD17B3酵素活性測定法の確立
矢澤隆志、佐藤貴弘、井田隆徳
第26回日本生殖内分泌学会学術集会 2022年1月8日
開催年月日： 2022年1月8日
記述言語：日本語 会議種別：口頭発表（一般）
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血中ナトリウム利尿ペプチド濃度と高血圧 発症
加藤 丈司，川越 由紀子，姜 丹鳳，松﨑 美南， 井田 隆徳，北村 和雄
第25回日本心血管内分泌学会学術総会 2021年12月10日
開催年月日： 2021年12月10日
記述言語：日本語 会議種別：口頭発表（一般）
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心血管内分泌学の未来を拓く探索研究 招待あり
井田隆徳
第25回日本心血管内分泌学会学術総会 2021年12月10日
開催年月日： 2021年12月10日
記述言語：日本語 会議種別：口頭発表（一般）
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モデル生物を用いた生理活性ペプチドの探索
井田隆徳、佐藤貴弘、児島将康
第25回日本心血管内分泌学会学術総会 2021年12月10日
開催年月日： 2021年12月10日
記述言語：日本語 会議種別：口頭発表（一般）
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線虫タキキニン様ペプチドの発見
井田隆徳
第１１回ペプチド・ホルモン研究会 2021年11月27日
開催年月日： 2021年11月27日
記述言語：日本語 会議種別：口頭発表（一般）
受賞 【 表示 ／ 非表示 】
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日本獣医学会生理学・生化学分科会奨励賞
2010年9月 日本獣医学会
井田隆徳
受賞区分：国内学会・会議・シンポジウム等の賞 受賞国：日本国
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11th International Conference on Human-Animal Interactions.Best Poster Award
2007年10月 IAHAIO
Yoshidomi Y, Ikeda Y, Satoh H, Sekino R, Ida T, Hisatomi I, Nakatsuka K, Misawa N
受賞区分：国際学会・会議・シンポジウム等の賞 受賞国：日本国
科研費（文科省・学振・厚労省）獲得実績 【 表示 ／ 非表示 】
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摂食・エネルギー代謝を制御する新規生理活性ペプチドの発見と応用
研究課題/領域番号：21K05958 2021年04月 - 2024年03月
科学研究費補助金 基盤研究C
担当区分：研究代表者
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難治性の神経因性疼痛を抑制する脳由来新規生理活性物質の構造決定と生理作用の解析
研究課題/領域番号：20K07768 2020年04月 - 2023年03月
科学研究費補助金 基盤研究(C)
担当区分：研究分担者
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新規NPY様RYamideペプチドの発見と食欲調節機構の解析
2017年04月 - 2020年03月
科学研究費補助金 基盤研究(C)
担当区分：研究代表者
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昆虫における食欲促進/減退を引き起こす生理活性ペプチドの発見
2014年04月 - 2017年03月
科学研究費補助金 基盤研究(C)
担当区分：研究代表者
昆虫における食欲促進/減退を引き起こす生理活性ペプチドの発見し解析する
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食欲・脂肪代謝調節に関係する新規生理活性ペプチドの探索
2013年04月 - 2015年03月
科学研究費補助金 新学術領域（研究領域提案型）
食欲・脂肪代謝調節に関係する新規生理活性ペプチドの探索し解析する
その他競争的資金獲得実績 【 表示 ／ 非表示 】
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新規生理活性ペプチドの探索
2010年01月 - 2015年03月
文部科学省 特色ある大学教育支援プログラム
担当区分：研究代表者 資金種別：競争的資金
新規生理活性ペプチドの探索
その他研究活動 【 表示 ／ 非表示 】
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食鳥処理施設での脳のサンプリング
2019年07月
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食鳥処理施設での肝臓のサンプリング
2018年06月
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食肉処理施設における膵臓のサンプリング
2017年07月
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食鳥処理施設での脳のサンプリング
2017年05月
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食鳥処理場における新規生理活性ペプチド、ステロイドホルモンの探索
2017年03月
食鳥処理場においてウシ、ブタ、ニワトリから様々な臓器をサンプリングし、新規生理活性ペプチド、ステロイドホルモンの探索を行う。