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Affiliation |
Faculty of Agriculture Department of Biochemistry and Applied Biosciences |
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Title |
Associate Professor |
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External Link |
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INOUE Kengo
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Degree 【 display / non-display 】
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Doctor (Agriculture) ( 2007.3 The University of Tokyo )
Research Areas 【 display / non-display 】
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Life Science / Applied microbiology
Papers 【 display / non-display 】
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Bioengineering study of electricity generation mechanisms in microbial fuel cells and their application in organic waste treatment Reviewed
Inoue Kengo
Seibutsu-kogaku Kaishi 102 ( 3 ) 109 - 115 2024.3
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：The Society for Biotechnology, Japan
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Performance of stacked microbial fuel cells with Barley–Shochu waste Reviewed
Fujimura S., Kamitori K., Kamei I., Nagamine M., Miyoshi K., Inoue K.
Journal of Bioscience and Bioengineering 2022.5
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Bioscience and Bioengineering
The treatment of barley–shochu waste combined with electricity generation was examined using stacked microbial fuel cells (SMFCs). The maximum chemical oxygen demand (CODCr) removal efficiency and maximum power density were achieved at 36.7 ± 1.1% and 4.3 ± 0.2 W m⁻³ (15.7 ± 0.9 mW m−2). The acetic acid concentration in effluent increased, whereas the citric acid, ethanol and sugar concentrations decreased during the operation. Microbial community analysis of the anode cell suspension and raw barley–shochu waste revealed that Clostridiaceae, Acetobacteraceae, and Enterobacteriaceae became predominant after the operation, implying that microorganisms belonging to these families might be involved in organic waste decomposition and electricity generation in the SMFCs.
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Unexpected genomic features of high current density-producing Geobacter sulfurreducens strain YM18 Reviewed
Fujikawa T., Ogura Y., Ishigami K., Kawano Y., Nagamine M., Hayashi T., Inoue K.
FEMS microbiology letters 368 ( 17 ) 2021.9
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：FEMS microbiology letters
Geobacter sulfurreducens produces high current densities and it has been used as a model organism for extracellular electron transfer studies. Nine G. sulfurreducens strains were isolated from biofilms formed on an anode poised at -0.2 V (vs SHE) in a bioelectrochemical system in which river sediment was used as an inoculum. The maximum current density of an isolate, strain YM18 (9.29 A/m2), was higher than that of the strain PCA (5.72 A/m2), the type strain of G. sulfurreducens, and comparable to strain KN400 (8.38 A/m2), which is another high current-producing strain of G. sulfurreducens. Genomic comparison of strains PCA, KN400 and YM18 revealed that omcB, xapD, spc and ompJ, which are known to be important genes for iron reduction and current production in PCA, were not present in YM18. In the PCA and KN400 genomes, two and one region(s) encoding CRISPR/Cas systems were identified, respectively, but they were missing in the YM18 genome. These results indicate that there is genetic variation in the key components involved in extracellular electron transfer among G. sulfurreducens strains.
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Fujikawa T., Ogura Y., Hayashi T., Inoue K.
Microbiology Resource Announcements 10 ( 33 ) 2021.8
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Microbiology Resource Announcements
Here, we report the complete genome sequence of Geobacter sulfurreducens strain YM35, which was isolated from biofilms formed on an anode in a bioelectrochemical system where river sediment was used as an inoculum. The chromosome is 3,745,223bp with a G1C content of 60.9%. The chromosome contains 3,324 protein-coding genes.
DOI： 10.1128/MRA.00539-21
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Ashikawa Y., Fujimoto Z., Inoue K., Yamane H., Nojiri H.
Acta Crystallographica Section D: Structural Biology 77 921 - 932 2021.7
Language：English Publishing type：Research paper (scientific journal) Publisher：Acta Crystallographica Section D: Structural Biology
Carbazole 1,9a-dioxygenase (CARDO), which consists of an oxygenase component and the electron-transport components ferredoxin (CARDO-F) and ferredoxin reductase (CARDO-R), is a Rieske nonheme iron oxygenase (RO). ROs are classified into five subclasses (IA, IB, IIA, IIB and III) based on their number of constituents and the nature of their redox centres. In this study, two types of crystal structure (type I and type II) were resolved of the class III CARDO-R from Janthinobacterium sp. J3 (CARDO-RJ3). Superimposition of the type I and type II structures revealed the absence of flavin adenine dinucleotide (FAD) in the type II structure along with significant conformational changes to the FAD-binding domain and the C-terminus, including movements to fill the space in which FAD had been located. Docking simulation of NADH into the FAD-bound form of CARDO-RJ3 suggested that shifts of the residues at the C-terminus caused the nicotinamide moiety to approach the N5 atom of FAD, which might facilitate electron transfer between the redox centres. Differences in domain arrangement were found compared with RO reductases from the ferredoxin-NADP reductase family, suggesting that these differences correspond to differences in the structures of their redox partners ferredoxin and terminal oxygenase. The results of docking simulations with the redox partner class III CARDO-F from Pseudomonas resinovorans CA10 suggested that complex formation suitable for efficient electron transfer is stabilized by electrostatic attraction and complementary shapes of the interacting regions.
Books 【 display / non-display 】
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微生物を活用した有用物質の製造技術
井上謙吾（ Role： Contributor , 微生物発電）
シーエムシー出版 2023.5
Language：Japanese Book type：Scholarly book
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微生物を用いた発電および水素生産
井上謙吾（ Role： Contributor , Geobacter属細菌の発電機構と応用）
シーエムシー出版 2021.11
Language：Japanese Book type：Scholarly book
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バイオエネルギー再燃
井上謙吾（ Role： Contributor , 芋焼酎粕を用いた微生物燃料電池の開発）
シーエムシー出版 2021.11
Language：Japanese Book type：Scholarly book
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Electron-Based Bioscience and Biotechnology
Takashi Fujikawa, Kengo Inoue（ Role： Contributor）
Springer 2020.9
Language：English Book type：Scholarly book
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バイオマスエネルギーの技術と市場
井上謙吾（ Role： Joint author）
シーエムシー出版 2016.10
Language：Japanese Book type：Scholarly book
MISC 【 display / non-display 】
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電気化学活性微生物による有機性廃棄物の有効利用 Invited Reviewed
井上謙吾
生物工学会誌 99 ( 12 ) 623 - 626 2021.12
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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微生物燃料電池を用いた未利用バイオマス発電 Invited Reviewed
井上謙吾
日本海水学会誌 74 ( 1 ) 9 - 14 2020.2
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：日本海水学会
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Electricity Generation Using Shochu-waste by Microbial Fuel Cells Invited Reviewed
77 ( 4 ) 2019.7
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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嫌気性微生物による発電と汚染物質分解～マサチューセッツ大学での微生物燃料電池研究で得たもの～ Invited
井上謙吾
日本微生物生態学会誌 32 ( 2 ) 61 - 63 2017.10
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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微生物生態と水環境工学に関する最新研究動向 Invited
栗栖太、伊藤司、青井議輝、久保田健吾、佐藤弘泰、金田一智規、井上謙吾、寺田昭彦
日本水環境学会誌 39 444 - 451 2016
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
Presentations 【 display / non-display 】
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未知の発電菌を求めて Invited
井上謙吾
日本科学未来館シンポジウム発電菌の研究：未来に向けて 2024.1.21
Event date： 2024.1.21
Language：Japanese Presentation type：Oral presentation (general)
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Klebsiella属細菌による嫌気性ピレン分解経路の解明
中川豊栄、七田実咲、亀井一郎、井上謙吾
第29回生物工学会九州支部福岡大会 2023.12.3
Event date： 2023.12.3
Language：Japanese Presentation type：Oral presentation (general)
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曝気と微生物燃料電池による養豚廃水の処理性能と微生物群集構造の変化
長嶺美幸、Nelly Wira Nurhadi、Mochammad Wachid、小林弘明、飯田和輝、井上謙吾
第29回生物工学会九州支部福岡大会 2023.12.3
Event date： 2023.12.3
Language：Japanese Presentation type：Oral presentation (general)
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微生物の細胞外電子伝達能力とその応用 Invited
井上謙吾
日本生物工学会九州支部会創立30周年記念講演会 2023.12.2
Event date： 2023.12.2
Language：Japanese Presentation type：Oral presentation (general)
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新規電流生成微生物の探索 Invited
井上謙吾
第36回日本微生態学会シンポジウム 2023.11.30
Event date： 2023.11.28 - 2023.11.30
Language：Japanese Presentation type：Oral presentation (general)
Awards 【 display / non-display 】
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日本生物工学会奨励賞（照井賞）
2023.9 日本生物工学会 微生物燃料電池の発電機構の解明と有機性廃棄物処理への応用に関する生物工学研究
井上謙吾
Award type：Award from Japanese society, conference, symposium, etc.
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ハイステップ研究者
2018.1 宮崎大学
井上謙吾
Country：Japan
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日本農芸化学会西日本市部会奨励賞
2017.1 日本農芸化学会西日本支部会
井上謙吾
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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ハイステップ研究者
2014.10 宮崎大学
井上謙吾
Country：Japan
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極限環境生物学会奨励賞
2012.12 極限環境生物学会
井上謙吾
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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Geobacter属細菌由来電気伝導性ナノワイヤーの機能解析
Grant number：22K05385 2022.04 - 2025.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
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微生物に秘められた驚くべき力
Grant number：HT30267 2018.11
日本学術振興会 科研費補助金 ひらめき☆ときめきサイエンス
Authorship：Principal investigator
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Geobacter属細菌の細胞外電子伝達ネットワークの解明
2016.04 - 2019.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
Geobacter属細菌の細胞外電子伝達ネットワークの解明
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鉄還元細菌のマルチヘムc型シトクロムの生産・分泌機構の解明
2014.04 - 2016.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
鉄還元細菌のマルチヘムc型シトクロムの生産・分泌機構の解明
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ヘテロ環芳香族化合物の嫌気的分解経路の解明
2012.04 - 2014.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
ヘテロ環芳香族化合物の嫌気的分解経路の解明
Available Technology 【 display / non-display 】
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微生物燃料電池の発電メカニズムの解明
微生物燃料電池による有機性廃棄物を利用した発電
環境浄化に向けた有用微生物の探索Related fields where technical consultation is available：微生物の分離・培養、嫌気性微生物、微生物分解
Message：微生物燃料電池は適用できる有機化合物の範囲が広く、含水率が高い廃棄物を含め、あらゆる有機性廃棄物を燃料化できます。嫌気性の汚染物質分解についても精力的に研究を行っております。ご質問ご相談などがある方は、お気軽にご連絡ください（kinoue@cc.miyazaki-u.ac.jp）。