INOUE Kengo

写真a

Affiliation

Faculty of Agriculture Department of Biochemistry and Applied Biosciences

Title

Associate Professor

External Link

Degree 【 display / non-display

  • Doctor (Agriculture) ( 2007.3   The University of Tokyo )

Research Areas 【 display / non-display

  • Life Science / Applied microbiology

 

Papers 【 display / non-display

  • Performance of stacked microbial fuel cells with Barley–Shochu waste Reviewed

    Fujimura S., Kamitori K., Kamei I., Nagamine M., Miyoshi K., Inoue K.

    Journal of Bioscience and Bioengineering   2022.5

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Journal of Bioscience and Bioengineering  

    The treatment of barley–shochu waste combined with electricity generation was examined using stacked microbial fuel cells (SMFCs). The maximum chemical oxygen demand (CODCr) removal efficiency and maximum power density were achieved at 36.7 ± 1.1% and 4.3 ± 0.2 W m⁻³ (15.7 ± 0.9 mW m−2). The acetic acid concentration in effluent increased, whereas the citric acid, ethanol and sugar concentrations decreased during the operation. Microbial community analysis of the anode cell suspension and raw barley–shochu waste revealed that Clostridiaceae, Acetobacteraceae, and Enterobacteriaceae became predominant after the operation, implying that microorganisms belonging to these families might be involved in organic waste decomposition and electricity generation in the SMFCs.

    DOI: 10.1016/j.jbiosc.2022.02.004

    Scopus

  • Unexpected genomic features of high current density-producing Geobacter sulfurreducens strain YM18 Reviewed

    Fujikawa T., Ogura Y., Ishigami K., Kawano Y., Nagamine M., Hayashi T., Inoue K.

    FEMS microbiology letters   368 ( 17 )   2021.9

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:FEMS microbiology letters  

    Geobacter sulfurreducens produces high current densities and it has been used as a model organism for extracellular electron transfer studies. Nine G. sulfurreducens strains were isolated from biofilms formed on an anode poised at -0.2 V (vs SHE) in a bioelectrochemical system in which river sediment was used as an inoculum. The maximum current density of an isolate, strain YM18 (9.29 A/m2), was higher than that of the strain PCA (5.72 A/m2), the type strain of G. sulfurreducens, and comparable to strain KN400 (8.38 A/m2), which is another high current-producing strain of G. sulfurreducens. Genomic comparison of strains PCA, KN400 and YM18 revealed that omcB, xapD, spc and ompJ, which are known to be important genes for iron reduction and current production in PCA, were not present in YM18. In the PCA and KN400 genomes, two and one region(s) encoding CRISPR/Cas systems were identified, respectively, but they were missing in the YM18 genome. These results indicate that there is genetic variation in the key components involved in extracellular electron transfer among G. sulfurreducens strains.

    DOI: 10.1093/femsle/fnab119

    Scopus

  • Complete genome sequence of high current-producing Geobacter sulfurreducens strain YM35, isolated from river sediment in Japan Reviewed

    Fujikawa T., Ogura Y., Hayashi T., Inoue K.

    Microbiology Resource Announcements   10 ( 33 )   2021.8

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Microbiology Resource Announcements  

    Here, we report the complete genome sequence of Geobacter sulfurreducens strain YM35, which was isolated from biofilms formed on an anode in a bioelectrochemical system where river sediment was used as an inoculum. The chromosome is 3,745,223bp with a G1C content of 60.9%. The chromosome contains 3,324 protein-coding genes.

    DOI: 10.1128/MRA.00539-21

    Scopus

  • Crystal structure of the ferredoxin reductase component of carbazole 1,9a-dioxygenase from Janthinobacterium sp. J3 Reviewed

    Ashikawa Y., Fujimoto Z., Inoue K., Yamane H., Nojiri H.

    Acta Crystallographica Section D: Structural Biology   77   921 - 932   2021.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Acta Crystallographica Section D: Structural Biology  

    Carbazole 1,9a-dioxygenase (CARDO), which consists of an oxygenase component and the electron-transport components ferredoxin (CARDO-F) and ferredoxin reductase (CARDO-R), is a Rieske nonheme iron oxygenase (RO). ROs are classified into five subclasses (IA, IB, IIA, IIB and III) based on their number of constituents and the nature of their redox centres. In this study, two types of crystal structure (type I and type II) were resolved of the class III CARDO-R from Janthinobacterium sp. J3 (CARDO-RJ3). Superimposition of the type I and type II structures revealed the absence of flavin adenine dinucleotide (FAD) in the type II structure along with significant conformational changes to the FAD-binding domain and the C-terminus, including movements to fill the space in which FAD had been located. Docking simulation of NADH into the FAD-bound form of CARDO-RJ3 suggested that shifts of the residues at the C-terminus caused the nicotinamide moiety to approach the N5 atom of FAD, which might facilitate electron transfer between the redox centres. Differences in domain arrangement were found compared with RO reductases from the ferredoxin-NADP reductase family, suggesting that these differences correspond to differences in the structures of their redox partners ferredoxin and terminal oxygenase. The results of docking simulations with the redox partner class III CARDO-F from Pseudomonas resinovorans CA10 suggested that complex formation suitable for efficient electron transfer is stabilized by electrostatic attraction and complementary shapes of the interacting regions.

    DOI: 10.1107/S2059798321005040

    Scopus

  • Proteolytic Maturation of the Outer Membrane c-Type Cytochrome OmcZ by a Subtilisin-Like Serine Protease Is Essential for Optimal Current Production by Geobacter sulfurreducens Reviewed International coauthorship

    Kai A., Tokuishi T., Fujikawa T., Kawano Y., Ueki T., Nagamine M., Sakakibara Y., Suiko M., Inoue K.

    Applied and environmental microbiology   87 ( 12 )   2021.5

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Applied and environmental microbiology  

    An outer membrane c-type cytochrome (OmcZ) in Geobacter sulfurreducens is essential for optimal current production in microbial fuel cells. OmcZ exists in two forms, small and large, designated OmcZS and OmcZL, respectively. However, it is still not known how these two structures are formed. A mutant with a disruption of the GSU2075 gene encoding a subtilisin-like serine protease (designated ozpA for the OmcZprotease), which is located downstream of omcZ, produced low currents at a level similar to that of the omcZ-deficient mutant strain. Biochemical analyses revealed that the ozpA mutant accumulated OmcZL and did not produce OmcZS, which is thought to be a mature form that is essential for the extracellular electron transfer to the electrode. A heterologous expression system cell lysate from an Escherichia coli strain producing OzpA cleaved OmcZL and generated OmcZS as the proteolytic product. Among the culture supernatant, loosely bound outer surface, and intracellular protein fractions from wild-type G. sulfurreducens, only the culture supernatant protein fraction showed OmcZL cleavage activity, indicating that the mature form of OmcZ, OmcZS, can be produced outside the cells. These results indicate that OzpA is an essential protease for current production via the maturation of OmcZ, and OmcZS is the key to the extracellular electron transfer to electrodes. This proteolytic maturation of OmcZ is a unique regulation among known c-type cytochromes in G. sulfurreducens. IMPORTANCE Microbial fuel cells are a promising technology for energy generation from various waste types. However, the molecular mechanisms of microbial extracellular electron transfer to the electrode need to be elucidated. G. sulfurreducens is a common key player in electricity generation in mixed-culture microbial fuel cell systems and a model microorganism for the study of extracellular electron transfer. Outer membrane c-type cytochrome OmcZ is essential for an optimal current production by G. sulfurreducens. OmcZ proteolytic cleavage occurs during maturation, but the underlying mechanism is unknown. This study identifies a subtilisin-like protease, OzpA, which plays a role in cleaving OmcZ and generating the mature form of OmcZ (OmcZS). OzpA is essential for current production and, thus, the proteolytic maturation of OmcZ. This is a novel regulation of the c-type cytochrome for G. sulfurreducens extracellular electron transfer. This study also provides new insights into the design strategy and development of microbial extracellular electron transfer for an efficient energy conversion from chemical energy to electricity.

    DOI: 10.1128/AEM.02617-20

    Scopus

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Books 【 display / non-display

  • 微生物を用いた発電および水素生産

    井上謙吾( Role: Contributor ,  Geobacter属細菌の発電機構と応用)

    シーエムシー出版  2021.11 

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    Language:Japanese Book type:Scholarly book

  • バイオエネルギー再燃

    井上謙吾( Role: Contributor ,  芋焼酎粕を用いた微生物燃料電池の開発)

    シーエムシー出版  2021.11 

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    Language:Japanese Book type:Scholarly book

  • Electron-Based Bioscience and Biotechnology

    Takashi Fujikawa, Kengo Inoue( Role: Contributor)

    Springer  2020.9 

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    Language:English Book type:Scholarly book

  • バイオマスエネルギーの技術と市場

    井上謙吾( Role: Joint author)

    シーエムシー出版  2016.10 

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    Language:Japanese Book type:Scholarly book

  • Manual of Environmental Microbiology 4th edition

    Inoue, K., Pinyakong, O., Kasuga, K., Nojiri, H.( Role: Joint author)

    ASM Press  2016.5 

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    Language:English Book type:Scholarly book

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MISC 【 display / non-display

  • 電気化学活性微生物による有機性廃棄物の有効利用 Invited Reviewed

    井上謙吾

    生物工学会誌   99 ( 12 )   623 - 626   2021.12

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    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    DOI: 10.34565/seibutsukogaku.99.12_623

  • 微生物燃料電池を用いた未利用バイオマス発電 Invited Reviewed

    井上謙吾

    日本海水学会誌   74 ( 1 )   9 - 14   2020.2

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Publisher:日本海水学会  

  • Electricity Generation Using Shochu-waste by Microbial Fuel Cells Invited Reviewed

    77 ( 4 )   2019.7

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • 嫌気性微生物による発電と汚染物質分解~マサチューセッツ大学での微生物燃料電池研究で得たもの~ Invited

    井上謙吾

    日本微生物生態学会誌   32 ( 2 )   61 - 63   2017.10

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • 微生物生態と水環境工学に関する最新研究動向 Invited

    栗栖太、伊藤司、青井議輝、久保田健吾、佐藤弘泰、金田一智規、井上謙吾、寺田昭彦

    日本水環境学会誌   39   444 - 451   2016

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

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Presentations 【 display / non-display

  • 細菌の細胞外への電子伝達に関する研究 Invited

    井上謙吾

    第19回微生物研究会  2021.11.13 

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    Event date: 2021.11.13

    Language:Japanese   Presentation type:Oral presentation (general)  

  • Electrode Reduction by Geobacter sulfurreducens Invited International conference

    井上謙吾

    International Conference on Materials and Systems for Sustainability 2021  2021.11.4 

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    Event date: 2021.11.4 - 2021.11.6

    Language:English   Presentation type:Oral presentation (general)  

  • 細菌の細胞外への電子伝達メカニズム Invited

    井上謙吾

    第12回トランスポーター研究会九州支部会  2021.8.7 

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    Event date: 2021.8.7

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 地域資源バイオマスを利用した微生物燃料電池

    井上謙吾

    日本農芸化学会大会 

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    Event date: 2021.3.18 - 2021.3.21

    Language:Japanese   Presentation type:Oral presentation (general)  

  • II型分泌系遺伝子の欠失が Geobacter sulfurreducensの発電能力へ及ぼす影響

    石神滉己,藤川昂,井上謙吾

    日本フードファクター学会・農芸化学会西日本支部会合同大会 

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    Event date: 2020.11.28 - 2020.11.29

    Language:Japanese   Presentation type:Oral presentation (general)  

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Awards 【 display / non-display

  • ハイステップ研究者

    2018.1   宮崎大学  

    井上謙吾

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    Country:Japan

  • 日本農芸化学会西日本市部会奨励賞

    2017.1   日本農芸化学会西日本支部会  

    井上謙吾

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  • ハイステップ研究者

    2014.10   宮崎大学  

    井上謙吾

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    Country:Japan

  • 極限環境生物学会奨励賞

    2012.12   極限環境生物学会  

    井上謙吾

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  • 日本環境バイオテクノロジー学会奨励賞

    2012.6   日本環境バイオテクノロジー学会  

    井上謙吾

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

Grant-in-Aid for Scientific Research 【 display / non-display

  • 微生物に秘められた驚くべき力

    Grant number:HT30267  2018.11

    日本学術振興会  科研費補助金  ひらめき☆ときめきサイエンス

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    Authorship:Principal investigator 

  • Geobacter属細菌の細胞外電子伝達ネットワークの解明

    2016.04 - 2019.03

    科学研究費補助金  基盤研究(C)

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    Authorship:Principal investigator 

    Geobacter属細菌の細胞外電子伝達ネットワークの解明

  • 鉄還元細菌のマルチヘムc型シトクロムの生産・分泌機構の解明

    2014.04 - 2016.03

    科学研究費補助金  若手研究(B)

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    Authorship:Principal investigator 

    鉄還元細菌のマルチヘムc型シトクロムの生産・分泌機構の解明

  • ヘテロ環芳香族化合物の嫌気的分解経路の解明

    2012.04 - 2014.03

    科学研究費補助金  若手研究(B)

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    Authorship:Principal investigator 

    ヘテロ環芳香族化合物の嫌気的分解経路の解明

  • 鉄還元性多環芳香族化合物分解細菌の探索

    2010.09 - 2012.04

    科学研究費補助金  若手研究(スタートアップ)

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    鉄還元性多環芳香族化合物分解細菌の探索